MD15G1396600.v1.1

plant mutator transposase zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000251
Physical Location & Seq
Forward (+)
49621093 .. 49625407
4315 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1396600.v1.1.491

Sequence Viewer

Length: 1752 bp
ATGGAGGACCATACATTTGTTGTCGGTCAAGAGTTCCCTGATGTCAAGGCCTTTAGGAATGCAATTAAAGAAGCTGCAATTGCACAACACTTTGAGCTTCGCATCATAAAAAGTGACCTTATCCGCTACTTTGCAAAGTGTGCAACAGATGGCTGTCCATGGCGTATCCGGGCTGTGAAGCTTCCTAATGCCCCAACCTTTACAATAAGAAGCCTTGAAGGGACGCACACTTGTGGGAGAAATGCACACAATGGGCACCATCAGGCTTCCGTTGATTGGATTGTCAGTTTTATAGAAGAACGCCTGCGGGGTAACATCAATTACAAACCAAAGGATATATTGCACGACATCCATAAGCAATATGGGATTACTATACCGTATAAGCAAGCTTGGCGTGCAAAAGAAAGAGGACTTGCCGCAATTTATGGTTCTTCTGAGGAAGGGTATTGCCTTCTTCCATCATACTGCGAACAAATAAAGAAGACCAACCCTGGAAGCATTGCAGAGGTATTTACCACTGGTATAGATCACCGTTTCCACCGGTTGTTTGTGTCCTTTCATGCGTCCATTTATGGATTTCTGAATGGTTGCTTGCCTATCATTGGGCTTGGCGGAGTTCAGCTAAAAAGTAAATATCTCGGTACTTTACTCTCTGCAACTTCGTTTGATGCGGATGGTGGATTATTTCCACTTGCATATGGCGTTGTTGATGTAGAAAATGATGAAAGCTGGATGTGGTTCCTGTCAGAGTTGCATAAGGCACTAGAGATGAACAGTGAGAATATGCCGGAGCTCACATTTTTATCTGATGGACATAAGGGAATTGCAGATGCAATAAGAAGGAAGTTCCCAAATTCATTAAATGCTTTTTGCATGCGCCACTTGAGTGAAAGCATTGGAAAAGAGTTCAAGAACTCAAGGCTTGTTCATCTTCTATGGAATGCTGCATATGCCACTACCACTATTGGCTTTAAAGAAAAGATGGTTGAAATAGAGGAGGCATCTCCGGAAGCTGCAAAATGGCTACAACAATTTCATCCTTCCCGCTGGGCATTGGTTTATTTTGAAGGAACACGTTATGGCCATCTCTCATCAAATATTGAGGAGTTTAATAGATGGATTCTTGAAGCTCGTGAGCTACCCATAATCCAAGTGATTGAGACAATTCATATTAGATTGATGGCTGAGTTTGAAGAACGGCGTTTGAAGAGTAATTCGTGGGTTTCTGTACTTTCTCCGTCTGCTGAGAAGCGTATCATAGAAGCCATCAGCCATGCATCCACATATCAGGTCCTTCGGTCTGATCAAGTAGAATTTGAGGTTCTTTCACCAGATCGATCAGACATTGTGAATATTGGCACCCATTGTTGTTCCTGCCGTGATTGGCAACTATATGGGATACCATGTTCCCATGCTGTGGCTGCCCTCATCTCCTGTCAAAAAGACGTACACGCCTTTACACAGAAAGTTTTTACAGTTGCAAGTTACCGCGAAGCATATGCGCAGGAGATATACCCTATCCCAGGAAAAATTGAATGGAGAAAGACAGATGAGTCTTCTATGGATGACGATATCCAGGTTGTGCGACCTCCCAAGTTTCGTAGACAGCCAGGACGACCGGAAAAGAAACGAATTTGTGTAGAGGACCTGAATCGCGAGAAACATACTGTCCATTGTAGCCGGTGTAACCAAACTGGACATTATAAGACAACTTGCAAGGCAGAGGGTATTGAAAGTATCGAACAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

584

Amino Acids

66.66

Weight (kDa)

7.11

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBD_Tnp_Mut PF03108 4 - 69 7.4e-24 MuDR family transposase
MULE PF10551 208 - 296 4.4e-16 MULE transposase domain
SWIM PF04434 450 - 479 3.1e-11 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06740
fragaria_vesca FvH4_1g02210 FvH4_1g02210 FvH4_1g02210 FvH4_1g02210 FvH4_2g02692 FvH4_2g22241 FvH4_2g22241 FvH4_2g22241 FvH4_4g21561 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g02501 FvH4_6g02501
malus_domestica MD02G1020500.v1.1 MD04G1227400.v1.1 MD04G1236400.v1.1 MD05G1203600.v1.1 MD10G1190900.v1.1 MD12G1244100.v1.1 MD12G1255700.v1.1 MD13G1140100.v1.1 MD15G1396600.v1.1 MD16G1135600.v1.1
prunus_persica Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.6G346500_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G229900_v2.0.a1 Prupe.8G229900_v2.0.a1
pyrus_communis pycom02g01800 pycom02g16220 pycom04g20090 pycom04g21020 pycom04g21030 pycom05g19010 pycom10g16460 pycom12g18690 pycom12g22380 pycom12g23430 pycom13g12180 pycom15g14690 pycom15g23420 pycom15g35670 pycom16g11960
rosa_chinensis RchiOBHm_Chr2g0087301 RchiOBHm_Chr3g0448611 RchiOBHm_Chr3g0450381 RchiOBHm_Chr4g0427621 RchiOBHm_Chr6g0247111 RchiOBHm_Chr6g0289171 RchiOBHm_Chr7g0177141
rosa_laevigata RLG00000007221 RLG00000015852
rosa_multiflora Rmu_co8326617.1_g000001 Rmu_sc0003399.1_g000010
rosa_roxburghii Rroxscaffold_2G00153730 Rroxscaffold_5G00369250 Rroxscaffold_6G00426900 Rroxscaffold_6G00428110 Rroxscaffold_7G00179510 Rroxscaffold_7G00214340
rosa_rugosa Rorug01G0473800 Rorug01G0473900 Rorug01G0474000 Rorug02G0614200 Rorug02G0614300 Rorug02G0614400 Rorug02G0614500 Rorug02G0614600 Rorug02G0627200 Rorug02G0627300 Rorug02G0627400 Rorug02G0627500 Rorug05G0522400
rosa_samantha Rh2AG027500 Rh2BG026800 Rh2CG027900 Rh2DG027500 Rh3AG013600 Rh3AG027900 Rh3BG013400 Rh3BG028400 Rh3CG012500 Rh3CG027200 Rh3DG014200 Rh3DG028300 Rh4AG275400 Rh4BG281300 Rh4CG296600 Rh4DG278600 Rh6AG037400 Rh6AG037700 Rh6AG312600 Rh6BG032300 Rh6BG032600 Rh6BG319500 Rh6CG033400 Rh6CG326000 Rh6DG031100 Rh6DG311900 Rh7AG003400 Rh7CG003000 Rh7CG003100
rosa_wichuraiana Rw2G002170 Rw3G001000 Rw3G002170 Rw4G023880 Rw6G003210 Rw6G027010 Rw7G000280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1704
AasI GACNNNNNNGTC 1 cut(s) 1552
Acc16I TGCGCA 1 cut(s) 1503
AccB1I GGYRCC 2 cut(s) 255, 1358
AccB7I CCANNNNNTGG 1 cut(s) 1417
AccI GTMKAC 1 cut(s) 1603
AccII CGCG 2 cut(s) 1491, 1656
AccIII TCCGGA 1 cut(s) 1006
AciI CCGC 7 cut(s) 124, 307, 417, 612, 671, 1045, 1489
AcoI YGGCCR 1 cut(s) 1081
AcsI RAATTY 3 cut(s) 853, 1313, 1632
AfaI GTAC 3 cut(s) 643, 1230, 1449
AfiI CCNNNNNNNGG 4 cut(s) 276, 602, 1417, 1523
AflIII ACRYGT 1 cut(s) 1073
AgeI ACCGGT 1 cut(s) 540
AgsI TTSAA 9 cut(s) 218, 910, 989, 1067, 1127, 1193, 1207, 1535, 1733
AjnI CCWGG 4 cut(s) 490, 1522, 1575, 1609
AleI CACNNNNGTG 2 cut(s) 231, 885
AloI GAACNNNNNNTCC 2 cut(s) 1390, 1422
Alw21I GWGCWC 1 cut(s) 795
Alw26I GTCTC 1 cut(s) 1154
Aor13HI TCCGGA 1 cut(s) 1006
AoxI GGCC 2 cut(s) 48, 1081
ApeKI GCWGC 4 cut(s) 74, 944, 1013, 1421
ApoI RAATTY 3 cut(s) 853, 1313, 1632
ArsI GACNNNNNNTTYG 1 cut(s) 31
AsiGI ACCGGT 1 cut(s) 540
AspLEI GCGC 2 cut(s) 879, 1504
AspS9I GGNCC 3 cut(s) 7, 1291, 1645
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 2 cut(s) 521, 1320
AvaII GGWCC 3 cut(s) 7, 1291, 1645
BaeGI GKGCMC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 1083
BanI GGYRCC 2 cut(s) 255, 1358
BanII GRGCYC 1 cut(s) 795
BauI CACGAG 1 cut(s) 1131
BbsI GAAGAC 2 cut(s) 488, 1548
Bbv12I GWGCWC 1 cut(s) 795
BbvI GCAGC 4 cut(s) 61, 931, 1000, 1408
BceAI ACGGC 2 cut(s) 1214, 1362
BcgI CGANNNNNNTGC 2 cut(s) 1481, 1515
BciT130I CCWGG 4 cut(s) 492, 1524, 1577, 1611
BciVI GTATCC 2 cut(s) 176, 1392
BclI TGATCA 1 cut(s) 1303
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 1154
BfaI CTAG 2 cut(s) 764, 1750
BfuI GTATCC 2 cut(s) 176, 1392
BisI GCNGC 5 cut(s) 75, 417, 945, 1014, 1422
BlsI GCNGC 5 cut(s) 76, 418, 946, 1015, 1423
Bme1390I CCNGG 5 cut(s) 170, 492, 1524, 1577, 1611
Bme18I GGWCC 3 cut(s) 7, 1291, 1645
BmgT120I GGNCC 3 cut(s) 7, 1291, 1645
BmiI GGNNCC 3 cut(s) 257, 740, 1360
BmrFI CCNGG 5 cut(s) 170, 492, 1524, 1577, 1611
BmsI GCATC 5 cut(s) 111, 658, 820, 1010, 1286
BpiI GAAGAC 2 cut(s) 488, 1548
BpuEI CTTGAG 2 cut(s) 901, 904
BpuMI CCSGG 1 cut(s) 170
Bsa29I ATCGAT 1 cut(s) 1336
BsaJI CCNNGG 3 cut(s) 158, 490, 1522
BsaWI WCCGGW 3 cut(s) 540, 1006, 1618
Bsc4I CCNNNNNNNGG 4 cut(s) 276, 602, 1417, 1523
Bse118I RCCGGY 2 cut(s) 540, 1680
Bse1I ACTGG 2 cut(s) 523, 1699
Bse3DI GCAATG 1 cut(s) 498
BseAI TCCGGA 1 cut(s) 1006
BseBI CCWGG 4 cut(s) 492, 1524, 1577, 1611
BseCI ATCGAT 1 cut(s) 1336
BseDI CCNNGG 3 cut(s) 158, 490, 1522
BseGI GGATG 6 cut(s) 348, 679, 738, 1036, 1277, 1570
BseLI CCNNNNNNNGG 4 cut(s) 276, 602, 1417, 1523
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 3 cut(s) 426, 1176, 1236
BseNI ACTGG 2 cut(s) 523, 1699
BseRI GAGGAG 2 cut(s) 1010, 1118
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 4 cut(s) 61, 931, 1000, 1408
BseYI CCCAGC 1 cut(s) 1047
Bsh1236I CGCG 2 cut(s) 1491, 1656
Bsh1285I CGRYCG 1 cut(s) 1619
BshFI GGCC 2 cut(s) 50, 1083
BshNI GGYRCC 2 cut(s) 255, 1358
BshTI ACCGGT 1 cut(s) 540
BshVI ATCGAT 1 cut(s) 1336
BsiEI CGRYCG 1 cut(s) 1619
BsiHKAI GWGCWC 1 cut(s) 795
BsiSI CCGG 6 cut(s) 169, 541, 788, 1007, 1619, 1681
BslFI GGGAC 1 cut(s) 235
BslI CCNNNNNNNGG 4 cut(s) 276, 602, 1417, 1523
BsmAI GTCTC 1 cut(s) 1154
BsmFI GGGAC 1 cut(s) 235
BsmI GAATGC 2 cut(s) 64, 946
BsnI GGCC 2 cut(s) 50, 1083
Bsp1286I GDGCHC 2 cut(s) 258, 795
Bsp13I TCCGGA 1 cut(s) 1006
Bsp143I GATC 4 cut(s) 526, 1303, 1333, 1337
Bsp19I CCATGG 1 cut(s) 158
Bsp68I TCGCGA 1 cut(s) 1656
BspACI CCGC 7 cut(s) 124, 307, 417, 612, 671, 1045, 1489
BspANI GGCC 2 cut(s) 50, 1083
BspCNI CTCAG 3 cut(s) 427, 1177, 1237
BspDI ATCGAT 1 cut(s) 1336
BspEI TCCGGA 1 cut(s) 1006
BspFNI CGCG 2 cut(s) 1491, 1656
BspLI GGNNCC 3 cut(s) 257, 740, 1360
BspT107I GGYRCC 2 cut(s) 255, 1358
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 2 cut(s) 540, 1680
BsrI ACTGG 2 cut(s) 523, 1699
BssAI RCCGGY 2 cut(s) 540, 1680
BssECI CCNNGG 3 cut(s) 158, 490, 1522
BssMI GATC 4 cut(s) 526, 1303, 1333, 1337
BssSI CACGAG 1 cut(s) 1131
BssT1I CCWWGG 1 cut(s) 158
Bst2BI CACGAG 1 cut(s) 1131
Bst2UI CCWGG 4 cut(s) 492, 1524, 1577, 1611
Bst4CI ACNGT 6 cut(s) 378, 533, 776, 1477, 1669, 1746
Bst6I CTCTTC 1 cut(s) 1202
BstAPI GCANNNNNTGC 1 cut(s) 140
BstC8I GCNNGC 5 cut(s) 305, 387, 396, 593, 875
BstDEI CTNAG 3 cut(s) 435, 1185, 1245
BstDSI CCRYGG 1 cut(s) 158
BstENI CCTNNNNNAGG 1 cut(s) 1521
BstF5I GGATG 6 cut(s) 348, 679, 738, 1036, 1277, 1570
BstFNI CGCG 2 cut(s) 1491, 1656
BstHHI GCGC 2 cut(s) 879, 1504
BstKTI GATC 4 cut(s) 529, 1306, 1336, 1340
BstMAI GTCTC 1 cut(s) 1154
BstMBI GATC 4 cut(s) 526, 1303, 1333, 1337
BstMCI CGRYCG 1 cut(s) 1619
BstMWI GCNNNNNNNGC 6 cut(s) 80, 140, 391, 395, 950, 1421
BstNI CCWGG 4 cut(s) 492, 1524, 1577, 1611
BstNSI RCATGY 1 cut(s) 877
BstSCI CCNGG 5 cut(s) 168, 490, 1522, 1575, 1609
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 2 cut(s) 1491, 1656
BstV1I GCAGC 4 cut(s) 61, 931, 1000, 1408
BstV2I GAAGAC 2 cut(s) 488, 1548
Bsu15I ATCGAT 1 cut(s) 1336
BsuI GTATCC 2 cut(s) 176, 1392
BsuRI GGCC 2 cut(s) 50, 1083
BsuTUI ATCGAT 1 cut(s) 1336
BtgI CCRYGG 1 cut(s) 158
BtsCI GGATG 6 cut(s) 348, 679, 738, 1036, 1277, 1570
BtsIMutI CAGTG 2 cut(s) 516, 781
BtuMI TCGCGA 1 cut(s) 1656
Cac8I GCNNGC 5 cut(s) 305, 387, 396, 593, 875
CfoI GCGC 2 cut(s) 879, 1504
Cfr10I RCCGGY 2 cut(s) 540, 1680
Cfr13I GGNCC 3 cut(s) 7, 1291, 1645
ClaI ATCGAT 1 cut(s) 1336
CseI GACGC 2 cut(s) 232, 552
Csp6I GTAC 3 cut(s) 642, 1229, 1448
CspAI ACCGGT 1 cut(s) 540
CviAII CATG 6 cut(s) 159, 560, 874, 1274, 1404, 1412
CviQI GTAC 3 cut(s) 642, 1229, 1448
DdeI CTNAG 3 cut(s) 435, 1185, 1245
DpnI GATC 4 cut(s) 528, 1305, 1335, 1339
DpnII GATC 4 cut(s) 526, 1303, 1333, 1337
DraI TTTAAA 1 cut(s) 973
DrdI GACNNNNNNGTC 1 cut(s) 1552
DseDI GACNNNNNNGTC 1 cut(s) 1552
EaeI YGGCCR 1 cut(s) 1081
Eam1104I CTCTTC 1 cut(s) 1202
EarI CTCTTC 1 cut(s) 1202
EciI GGCGGA 1 cut(s) 627
Ecl136II GAGCTC 1 cut(s) 793
Eco130I CCWWGG 1 cut(s) 158
Eco147I AGGCCT 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 795
Eco32I GATATC 1 cut(s) 1573
Eco47I GGWCC 3 cut(s) 7, 1291, 1645
Eco53kI GAGCTC 1 cut(s) 793
EcoICRI GAGCTC 1 cut(s) 793
EcoNI CCTNNNNNAGG 1 cut(s) 1521
EcoO109I RGGNCCY 2 cut(s) 1291, 1645
EcoRII CCWGG 4 cut(s) 490, 1522, 1575, 1609
EcoRV GATATC 1 cut(s) 1573
EcoT14I CCWWGG 1 cut(s) 158
EcoT22I ATGCAT 1 cut(s) 1279
EcoT38I GRGCYC 1 cut(s) 795
ErhI CCWWGG 1 cut(s) 158
FaeI CATG 6 cut(s) 162, 563, 877, 1277, 1407, 1415
FaqI GGGAC 1 cut(s) 235
FatI CATG 6 cut(s) 158, 559, 873, 1273, 1403, 1411
FauI CCCGC 2 cut(s) 300, 1052
FauNDI CATATG 3 cut(s) 697, 949, 1498
FbaI TGATCA 1 cut(s) 1303
FblI GTMKAC 1 cut(s) 1603
Fnu4HI GCNGC 5 cut(s) 75, 417, 945, 1014, 1422
FokI GGATG 6 cut(s) 335, 686, 745, 1023, 1264, 1577
FriOI GRGCYC 1 cut(s) 795
Fsp4HI GCNGC 5 cut(s) 75, 417, 945, 1014, 1422
FspBI CTAG 2 cut(s) 764, 1750
FspI TGCGCA 1 cut(s) 1503
GlaI GCGC 2 cut(s) 878, 1503
GluI GCNGC 5 cut(s) 75, 417, 945, 1014, 1422
GsaI CCCAGC 1 cut(s) 1051
HaeIII GGCC 2 cut(s) 50, 1083
HapII CCGG 6 cut(s) 169, 541, 788, 1007, 1619, 1681
HgaI GACGC 2 cut(s) 232, 552
HhaI GCGC 2 cut(s) 879, 1504
Hin1II CATG 6 cut(s) 162, 563, 877, 1277, 1407, 1415
Hin6I GCGC 2 cut(s) 877, 1502
HinP1I GCGC 2 cut(s) 877, 1502
HindIII AAGCTT 2 cut(s) 179, 387
HinfI GANTC 3 cut(s) 1120, 1553, 1651
HpaII CCGG 6 cut(s) 169, 541, 788, 1007, 1619, 1681
HphI GGTGA 2 cut(s) 521, 1320
Hpy166II GTNNAC 2 cut(s) 1450, 1604
Hpy188I TCNGA 6 cut(s) 436, 582, 748, 808, 1303, 1342
Hpy188III TCNNGA 6 cut(s) 29, 910, 1007, 1124, 1133, 1655
Hpy8I GTNNAC 2 cut(s) 1450, 1604
HpyAV CCTTC 7 cut(s) 212, 434, 461, 834, 1050, 1061, 1304
HpyCH4III ACNGT 6 cut(s) 378, 533, 776, 1477, 1669, 1746
HpyCH4IV ACGT 2 cut(s) 1075, 1446
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 140, 391, 395, 950, 1421
HpyF3I CTNAG 3 cut(s) 435, 1185, 1245
HpySE526I ACGT 2 cut(s) 1075, 1446
Hsp92II CATG 6 cut(s) 162, 563, 877, 1277, 1407, 1415
HspAI GCGC 2 cut(s) 877, 1502
Kpn2I TCCGGA 1 cut(s) 1006
Ksp22I TGATCA 1 cut(s) 1303
Kzo9I GATC 4 cut(s) 526, 1303, 1333, 1337
LmnI GCTCC 1 cut(s) 790
Lsp1109I GCAGC 4 cut(s) 61, 931, 1000, 1408
LweI GCATC 5 cut(s) 111, 658, 820, 1010, 1286
MaeI CTAG 2 cut(s) 764, 1750
MaeII ACGT 2 cut(s) 1075, 1446
MaeIII GTNAC 4 cut(s) 113, 311, 1484, 1685
MalI GATC 4 cut(s) 528, 1305, 1335, 1339
MboI GATC 4 cut(s) 526, 1303, 1333, 1337
MboII GAAGA 8 cut(s) 308, 423, 446, 493, 923, 1205, 1219, 1548
MfeI CAATTG 1 cut(s) 78
MhlI GDGCHC 2 cut(s) 258, 795
MlsI TGGCCA 1 cut(s) 1083
MluNI TGGCCA 1 cut(s) 1083
MlyI GAGTC 1 cut(s) 1562
Mox20I TGGCCA 1 cut(s) 1083
Mph1103I ATGCAT 1 cut(s) 1279
MroI TCCGGA 1 cut(s) 1006
MscI TGGCCA 1 cut(s) 1083
MseI TTAA 4 cut(s) 66, 860, 972, 1110
MslI CAYNNNNRTG 2 cut(s) 231, 885
Msp20I TGGCCA 1 cut(s) 1083
MspA1I CMGCKG 1 cut(s) 1047
MspI CCGG 6 cut(s) 169, 541, 788, 1007, 1619, 1681
MspR9I CCNGG 5 cut(s) 170, 492, 1524, 1577, 1611
MunI CAATTG 1 cut(s) 78
Mva1269I GAATGC 2 cut(s) 64, 946
MvaI CCWGG 4 cut(s) 492, 1524, 1577, 1611
MvnI CGCG 2 cut(s) 1491, 1656
MwoI GCNNNNNNNGC 6 cut(s) 80, 140, 391, 395, 950, 1421
NciI CCSGG 1 cut(s) 170
NcoI CCATGG 1 cut(s) 158
NdeI CATATG 3 cut(s) 697, 949, 1498
NdeII GATC 4 cut(s) 526, 1303, 1333, 1337
NlaIII CATG 6 cut(s) 162, 563, 877, 1277, 1407, 1415
NlaIV GGNNCC 3 cut(s) 257, 740, 1360
NmuCI GTSAC 1 cut(s) 113
NruI TCGCGA 1 cut(s) 1656
NsbI TGCGCA 1 cut(s) 1503
NsiI ATGCAT 1 cut(s) 1279
NspI RCATGY 1 cut(s) 877
OliI CACNNNNGTG 2 cut(s) 231, 885
PaeI GCATGC 1 cut(s) 877
PceI AGGCCT 1 cut(s) 50
PctI GAATGC 2 cut(s) 64, 946
PfeI GAWTC 2 cut(s) 1120, 1651
PflMI CCANNNNNTGG 1 cut(s) 1417
PinAI ACCGGT 1 cut(s) 540
PkrI GCNGC 5 cut(s) 76, 418, 946, 1015, 1423
PleI GAGTC 1 cut(s) 1561
PpsI GAGTC 1 cut(s) 1561
PpuMI RGGWCCY 2 cut(s) 1291, 1645
PsiI TTATAA 1 cut(s) 1704
Psp124BI GAGCTC 1 cut(s) 795
Psp5II RGGWCCY 2 cut(s) 1291, 1645
Psp6I CCWGG 4 cut(s) 490, 1522, 1575, 1609
PspFI CCCAGC 1 cut(s) 1047
PspGI CCWGG 4 cut(s) 490, 1522, 1575, 1609
PspN4I GGNNCC 3 cut(s) 257, 740, 1360
PspPI GGNCC 3 cut(s) 7, 1291, 1645
PspPPI RGGWCCY 2 cut(s) 1291, 1645
RruI TCGCGA 1 cut(s) 1656
RsaI GTAC 3 cut(s) 643, 1230, 1449
RsaNI GTAC 3 cut(s) 642, 1229, 1448
RseI CAYNNNNRTG 2 cut(s) 231, 885
SacI GAGCTC 1 cut(s) 795
SaqAI TTAA 4 cut(s) 66, 860, 972, 1110
SatI GCNGC 5 cut(s) 75, 417, 945, 1014, 1422
Sau3AI GATC 4 cut(s) 526, 1303, 1333, 1337
Sau96I GGNCC 3 cut(s) 7, 1291, 1645
SchI GAGTC 1 cut(s) 1562
ScrFI CCNGG 5 cut(s) 170, 492, 1524, 1577, 1611
SduI GDGCHC 2 cut(s) 258, 795
SfaNI GCATC 5 cut(s) 111, 658, 820, 1010, 1286
SinI GGWCC 3 cut(s) 7, 1291, 1645
SmiMI CAYNNNNRTG 2 cut(s) 231, 885
SmlI CTYRAG 2 cut(s) 883, 916
SmoI CTYRAG 2 cut(s) 883, 916
SphI GCATGC 1 cut(s) 877
SseBI AGGCCT 1 cut(s) 50
SsiI CCGC 7 cut(s) 124, 307, 417, 612, 671, 1045, 1489
SspI AATATT 2 cut(s) 1099, 1354
SspMI CTAG 2 cut(s) 764, 1750
SstI GAGCTC 1 cut(s) 795
StuI AGGCCT 1 cut(s) 50
StyD4I CCNGG 5 cut(s) 168, 490, 1522, 1575, 1609
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 6 cut(s) 378, 533, 776, 1477, 1669, 1746
TaiI ACGT 2 cut(s) 1078, 1449
TaqI TCGA 2 cut(s) 1336, 1740
TaqII GACCGA 2 cut(s) 14, 1287
TatI WGTACW 1 cut(s) 1228
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 2 cut(s) 1120, 1651
Tru1I TTAA 4 cut(s) 66, 860, 972, 1110
Tru9I TTAA 4 cut(s) 66, 860, 972, 1110
TscAI CASTG 2 cut(s) 523, 781
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 4 cut(s) 74, 944, 1013, 1421
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 7 cut(s) 548, 738, 785, 846, 917, 1025, 1157
TspGWI ACGGA 2 cut(s) 259, 1227
TspRI CASTG 2 cut(s) 523, 781
Van91I CCANNNNNTGG 1 cut(s) 1417
VpaK11BI GGWCC 3 cut(s) 7, 1291, 1645
XagI CCTNNNNNAGG 1 cut(s) 1521
XapI RAATTY 3 cut(s) 853, 1313, 1632
XceI RCATGY 1 cut(s) 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 359
XmiI GTMKAC 1 cut(s) 1603
XspI CTAG 2 cut(s) 764, 1750
Zsp2I ATGCAT 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.