MD13G1140100.v1.1

plant mutator transposase zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000251
Physical Location & Seq
Reverse (-)
10799293 .. 10802400
3108 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1140100.v1.1.491

Sequence Viewer

Length: 1932 bp
ATGGATAAGGATGGAAATTTATCGTACTCTGGGGGCGACGCTCATGCGATTGGCATTGATGAGAAAACACTGCTTGGTGATTTTAAGTCCGAAATAGAGGATATGTTTAATTGTAGTGCTGATACAATGTCCATCAAGTACTTCCTTCCCGGCAATAAGAAGACTCTCATTACGATCTCTAAAGACAAAGACTTGCAACGAATGGTCAATTTTCTCGGGGATACAGACACTGTCGATGTATTTGTCATGACAGAGGAAGCTGCTGTTCGAAATTTGTCCATCATGCCTGCTAGTAGGTCAAGCAGGACAACTGTATCGGAAGCAGTTGTTCCTTTTGTTGAGCCCGTTGATATGCGAGTTGATGCTGACAATACTGACAATGCTGATGGCCAAATTGATATGGAACTCCATGAAATCCCTTCGCTTCGTGTTCCTAGTTGTTCTAGTGATGATATGCATCATAAAGCTGCACAACAGTGGGAAAGCACCATCACTGGTGTGGACCAAAGGTTTAATAGTTTTAGCGAATTCCGAGAAGCATTGCATAAGTTTTCAATTGCACATGGATTTGCTTATAGGTATAAGAAAAATGACAGTCATCGTGTCACTGTCAAATGCAAAGGTCAAAATTGCCCATGGAGGATATATGCATCAAGGTTGTCTACAACCCAGTTGATTTGTATCAAGAAAATGAACACGGATCATACATGTGAAGGAGCTGCTGTGAAAGCTGGGTATCGGGCAACGAGAGGTTGGGTGGGAAGTATCATAAAGGAGAAGTTGAAAATTTCCCCAAACTACAAGCCAAAGGCTATAGCAGAGGACATTAAGCGGGAATACGGGATTCAGTTGAATTATTCTCAGGCATGGCGTGCTAAAGAGATTGCCAGGGAGCAGCTTCAAGGCTCCTATAAAGAGGCCTATAATCAGTTGCCGTATTTCTGCGAGAAGATAAAGGAAACTAATCCAGGGAGTTTTGCTTCATTTACTACTAAGGAAGACTCGAGCTTCCATCGTTTCTTTGTATCTTTCCATGCATCAATTGTGGGTTTCATAGAAGCTTGCCGCCCTCTAATTTTTCTTGATAGCGCTCCTCTGAACTCAAAATATCAAGGAGTTTTGTTGGCTGCAACAGCCGCAGATGCTGATGATGGTGTATTTCCGGTTGCATTTTCTGTTGTGGATAATGAGACTGATGAGAACTGGCACTGGTTCTTACTAGAACTGAAATTGGCAGTGGCAACATCTCAGCCGATCACATTTGTAGCAGATTTTCAGAATGGATTGAAAAATTCATTGCCCGAGGTATTTGATAAATGTTACCACTGCTATTGTTTGAGGCATCTTGCTGAAAAACTTAACAAGGACTTGAAGGGCCAATTTTCTCACGAGGCAAGACGATTCATGATCAATGATTTTTATGCGGCTGCTTATGCACCCACTCAGGAGGCTTTCCAGCGTAGTGCTGACAACATAAAAAATATTTCACCGGATGCTTACAATTGGGTCATACAAAGTGAGCCCGTGCACTGGGCTAATGCATTCTGCGGAGGAGGCAGGTACAACCACATGACATCGAACTTCGGACAGCAGTTCTACAGTTGGGTATCAGAGGCGCATGAGTTGCCAATTACGCAGATGATTGACGTACTACGAGGCAATATGATGGAAGCCTTCTATTCACGCGGGGTGGAGTCCAACCAATGGGTGACAAGATTAACACCATCTAAGGAGGAAAAGCTTCAAAAGGAAACAGAAATTGCTCGATCACTTCAAGTGTTACTCTCACATGGAAGCACATTCGAGGTTCGTGGAGAATCTGTCGACACTATTGACATCGACCATTGGGATTGCACCTGTAAGGGATGGCATCTCACTGGAACCCTTATGATTACTGCTCCAGATACTTCACAGTGGAGAGTTACCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE PB1 SWIM

Protein Analysis

644

Amino Acids

72.85

Weight (kDa)

5.83

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 4 - 73 2.4e-07 PB1 domain
DBD_Tnp_Mut PF03108 164 - 228 5.3e-25 MuDR family transposase
MULE PF10551 360 - 451 3.5e-15 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06740
fragaria_vesca FvH4_1g02210 FvH4_1g02210 FvH4_1g02210 FvH4_1g02210 FvH4_2g02692 FvH4_2g22241 FvH4_2g22241 FvH4_2g22241 FvH4_4g21561 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g02501 FvH4_6g02501
malus_domestica MD02G1020500.v1.1 MD04G1227400.v1.1 MD04G1236400.v1.1 MD05G1203600.v1.1 MD10G1190900.v1.1 MD12G1244100.v1.1 MD12G1255700.v1.1 MD13G1140100.v1.1 MD15G1396600.v1.1 MD16G1135600.v1.1
prunus_persica Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.6G346500_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G229900_v2.0.a1 Prupe.8G229900_v2.0.a1
pyrus_communis pycom02g01800 pycom02g16220 pycom04g20090 pycom04g21020 pycom04g21030 pycom05g19010 pycom10g16460 pycom12g18690 pycom12g22380 pycom12g23430 pycom13g12180 pycom15g14690 pycom15g23420 pycom15g35670 pycom16g11960
rosa_chinensis RchiOBHm_Chr2g0087301 RchiOBHm_Chr3g0448611 RchiOBHm_Chr3g0450381 RchiOBHm_Chr4g0427621 RchiOBHm_Chr6g0247111 RchiOBHm_Chr6g0289171 RchiOBHm_Chr7g0177141
rosa_laevigata RLG00000007221 RLG00000015852
rosa_multiflora Rmu_co8326617.1_g000001 Rmu_sc0003399.1_g000010
rosa_roxburghii Rroxscaffold_2G00153730 Rroxscaffold_5G00369250 Rroxscaffold_6G00426900 Rroxscaffold_6G00428110 Rroxscaffold_7G00179510 Rroxscaffold_7G00214340
rosa_rugosa Rorug01G0473800 Rorug01G0473900 Rorug01G0474000 Rorug02G0614200 Rorug02G0614300 Rorug02G0614400 Rorug02G0614500 Rorug02G0614600 Rorug02G0627200 Rorug02G0627300 Rorug02G0627400 Rorug02G0627500 Rorug05G0522400
rosa_samantha Rh2AG027500 Rh2BG026800 Rh2CG027900 Rh2DG027500 Rh3AG013600 Rh3AG027900 Rh3BG013400 Rh3BG028400 Rh3CG012500 Rh3CG027200 Rh3DG014200 Rh3DG028300 Rh4AG275400 Rh4BG281300 Rh4CG296600 Rh4DG278600 Rh6AG037400 Rh6AG037700 Rh6AG312600 Rh6BG032300 Rh6BG032600 Rh6BG319500 Rh6CG033400 Rh6CG326000 Rh6DG031100 Rh6DG311900 Rh7AG003400 Rh7CG003000 Rh7CG003100
rosa_wichuraiana Rw2G002170 Rw3G001000 Rw3G002170 Rw4G023880 Rw6G003210 Rw6G027010 Rw7G000280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1548
AccB7I CCANNNNNTGG 1 cut(s) 1704
AccI GTMKAC 2 cut(s) 662, 1824
AccII CGCG 1 cut(s) 1686
AciI CCGC 6 cut(s) 832, 1066, 1137, 1424, 1548, 1686
AclWI GGATC 1 cut(s) 708
AcoI YGGCCR 1 cut(s) 388
AcsI RAATTY 5 cut(s) 16, 271, 527, 786, 1291
AfaI GTAC 4 cut(s) 26, 140, 1562, 1650
AfeI AGCGCT 1 cut(s) 1090
AfiI CCNNNNNNNGG 2 cut(s) 1530, 1704
AflIII ACRYGT 1 cut(s) 707
AgsI TTSAA 8 cut(s) 555, 784, 853, 902, 1288, 1372, 1745, 1775
AjnI CCWGG 2 cut(s) 887, 967
AleI CACNNNNGTG 2 cut(s) 475, 497
AluBI AGCT 8 cut(s) 260, 467, 719, 731, 898, 1008, 1061, 1741
AluI AGCT 8 cut(s) 260, 467, 719, 731, 898, 1008, 1061, 1741
Alw21I GWGCWC 1 cut(s) 1530
Alw26I GTCTC 1 cut(s) 1184
Alw44I GTGCAC 1 cut(s) 1526
AlwI GGATC 1 cut(s) 708
AlwNI CAGNNNCTG 2 cut(s) 230, 1145
Ama87I CYCGRG 3 cut(s) 215, 1003, 1301
Aor51HI AGCGCT 1 cut(s) 1090
AoxI GGCC 3 cut(s) 388, 918, 1375
ApaLI GTGCAC 1 cut(s) 1526
ApeKI GCWGC 6 cut(s) 260, 467, 719, 895, 1127, 1427
ApoI RAATTY 5 cut(s) 16, 271, 527, 786, 1291
Asp700I GAANNNNTTC 1 cut(s) 1740
AspLEI GCGC 2 cut(s) 1091, 1618
AspS9I GGNCC 2 cut(s) 502, 1375
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 3 cut(s) 89, 1479, 1720
AsuII TTCGAA 1 cut(s) 268
AvaI CYCGRG 3 cut(s) 215, 1003, 1301
AvaII GGWCC 1 cut(s) 502
BaeGI GKGCMC 1 cut(s) 1530
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BalI TGGCCA 1 cut(s) 390
BanII GRGCYC 2 cut(s) 345, 1524
BauI CACGAG 1 cut(s) 1388
BbsI GAAGAC 2 cut(s) 167, 1005
Bbv12I GWGCWC 1 cut(s) 1530
BbvI GCAGC 6 cut(s) 247, 454, 706, 907, 1114, 1414
BceAI ACGGC 1 cut(s) 919
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BciT130I CCWGG 2 cut(s) 889, 969
BciVI GTATCC 1 cut(s) 214
BclI TGATCA 1 cut(s) 1407
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 1184
BfaI CTAG 4 cut(s) 291, 435, 444, 1220
BfmI CTRYAG 2 cut(s) 813, 1597
BfoI RGCGCY 1 cut(s) 1092
BfuAI ACCTGC 1 cut(s) 1548
BfuI GTATCC 1 cut(s) 214
BisI GCNGC 9 cut(s) 261, 468, 720, 896, 1066, 1128, 1137, 1425, 1428
BlsI GCNGC 9 cut(s) 262, 469, 721, 897, 1067, 1129, 1138, 1426, 1429
BmcAI AGTACT 1 cut(s) 140
Bme1390I CCNGG 3 cut(s) 150, 889, 969
Bme18I GGWCC 1 cut(s) 502
BmeT110I CYCGRG 3 cut(s) 215, 1003, 1301
BmgT120I GGNCC 2 cut(s) 502, 1375
BmiI GGNNCC 2 cut(s) 907, 1882
BmrFI CCNGG 3 cut(s) 150, 889, 969
BmrI ACTGGG 2 cut(s) 664, 1540
BmsI GCATC 8 cut(s) 352, 466, 659, 1046, 1132, 1351, 1483, 1879
BmuI ACTGGG 2 cut(s) 664, 1540
BpiI GAAGAC 2 cut(s) 167, 1005
BpmI CTGGAG 1 cut(s) 1884
Bpu14I TTCGAA 1 cut(s) 268
BpuMI CCSGG 1 cut(s) 150
BsaBI GATNNNNATC 2 cut(s) 456, 680
BsaJI CCNNGG 4 cut(s) 635, 888, 968, 1302
BsaWI WCCGGW 2 cut(s) 1162, 1489
BsaXI ACNNNNNCTCC 4 cut(s) 708, 738, 1806, 1836
Bsc4I CCNNNNNNNGG 2 cut(s) 1530, 1704
Bse1I ACTGG 6 cut(s) 499, 670, 1208, 1214, 1535, 1882
Bse3DI GCAATG 2 cut(s) 539, 1295
Bse8I GATNNNNATC 2 cut(s) 456, 680
BseBI CCWGG 2 cut(s) 889, 969
BseDI CCNNGG 4 cut(s) 635, 888, 968, 1302
BseGI GGATG 3 cut(s) 16, 1498, 1871
BseJI GATNNNNATC 2 cut(s) 456, 680
BseLI CCNNNNNNNGG 2 cut(s) 1530, 1704
BseMI GCAATG 2 cut(s) 539, 1295
BseMII CTCAG 3 cut(s) 875, 1262, 1457
BseNI ACTGG 6 cut(s) 499, 670, 1208, 1214, 1535, 1882
BseRI GAGGAG 2 cut(s) 1083, 1566
BseSI GKGCMC 1 cut(s) 1530
BseXI GCAGC 6 cut(s) 247, 454, 706, 907, 1114, 1414
BseYI CCCAGC 1 cut(s) 731
BsgI GTGCAG 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 1686
BshFI GGCC 3 cut(s) 390, 920, 1377
BsiHKAI GWGCWC 1 cut(s) 1530
BsiHKCI CYCGRG 3 cut(s) 215, 1003, 1301
BsiSI CCGG 3 cut(s) 150, 1163, 1490
BslI CCNNNNNNNGG 2 cut(s) 1530, 1704
BsmAI GTCTC 1 cut(s) 1184
BsmI GAATGC 1 cut(s) 1541
BsnI GGCC 3 cut(s) 390, 920, 1377
BsoBI CYCGRG 3 cut(s) 215, 1003, 1301
Bsp119I TTCGAA 1 cut(s) 268
Bsp1286I GDGCHC 3 cut(s) 345, 1524, 1530
Bsp143I GATC 5 cut(s) 174, 700, 1254, 1407, 1766
Bsp19I CCATGG 1 cut(s) 635
BspACI CCGC 6 cut(s) 832, 1066, 1137, 1424, 1548, 1686
BspANI GGCC 3 cut(s) 390, 920, 1377
BspCNI CTCAG 3 cut(s) 874, 1261, 1456
BspFNI CGCG 1 cut(s) 1686
BspHI TCATGA 2 cut(s) 246, 1404
BspLI GGNNCC 2 cut(s) 907, 1882
BspMI ACCTGC 1 cut(s) 1548
BspPI GGATC 1 cut(s) 708
BspT104I TTCGAA 1 cut(s) 268
BsrDI GCAATG 2 cut(s) 539, 1295
BsrI ACTGG 6 cut(s) 499, 670, 1208, 1214, 1535, 1882
BssECI CCNNGG 4 cut(s) 635, 888, 968, 1302
BssMI GATC 5 cut(s) 174, 700, 1254, 1407, 1766
BssSI CACGAG 1 cut(s) 1388
BssT1I CCWWGG 1 cut(s) 635
Bst2BI CACGAG 1 cut(s) 1388
Bst2UI CCWGG 2 cut(s) 889, 969
Bst4CI ACNGT 8 cut(s) 232, 313, 477, 596, 610, 1601, 1914, 1927
BstAPI GCANNNNNTGC 2 cut(s) 872, 1624
BstBI TTCGAA 1 cut(s) 268
BstC8I GCNNGC 3 cut(s) 288, 873, 1063
BstDEI CTNAG 5 cut(s) 861, 993, 1248, 1443, 1728
BstDSI CCRYGG 1 cut(s) 635
BstF5I GGATG 3 cut(s) 16, 1498, 1871
BstFNI CGCG 1 cut(s) 1686
BstH2I RGCGCY 1 cut(s) 1092
BstHHI GCGC 2 cut(s) 1091, 1618
BstKTI GATC 5 cut(s) 177, 703, 1257, 1410, 1769
BstMAI GTCTC 1 cut(s) 1184
BstMBI GATC 5 cut(s) 174, 700, 1254, 1407, 1766
BstMWI GCNNNNNNNGC 9 cut(s) 728, 872, 1133, 1136, 1142, 1433, 1554, 1624, 1633
BstNI CCWGG 2 cut(s) 889, 969
BstNSI RCATGY 1 cut(s) 711
BstSCI CCNGG 3 cut(s) 148, 887, 967
BstSFI CTRYAG 2 cut(s) 813, 1597
BstSLI GKGCMC 1 cut(s) 1530
BstUI CGCG 1 cut(s) 1686
BstV1I GCAGC 6 cut(s) 247, 454, 706, 907, 1114, 1414
BstV2I GAAGAC 2 cut(s) 167, 1005
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 3 cut(s) 390, 920, 1377
BtgI CCRYGG 1 cut(s) 635
BtsCI GGATG 3 cut(s) 16, 1498, 1871
BtsI GCAGTG 3 cut(s) 68, 1242, 1324
BveI ACCTGC 1 cut(s) 1548
Cac8I GCNNGC 3 cut(s) 288, 873, 1063
CaiI CAGNNNCTG 2 cut(s) 230, 1145
CciI TCATGA 2 cut(s) 246, 1404
CfoI GCGC 2 cut(s) 1091, 1618
Cfr13I GGNCC 2 cut(s) 502, 1375
CseI GACGC 1 cut(s) 47
Csp6I GTAC 4 cut(s) 25, 139, 1561, 1649
CspCI CAANNNNNGTGG 2 cut(s) 1313, 1348
CviQI GTAC 4 cut(s) 25, 139, 1561, 1649
DdeI CTNAG 5 cut(s) 861, 993, 1248, 1443, 1728
DpnI GATC 5 cut(s) 176, 702, 1256, 1409, 1768
DpnII GATC 5 cut(s) 174, 700, 1254, 1407, 1766
EaeI YGGCCR 1 cut(s) 388
Eco130I CCWWGG 1 cut(s) 635
Eco147I AGGCCT 1 cut(s) 920
Eco24I GRGCYC 2 cut(s) 345, 1524
Eco47I GGWCC 1 cut(s) 502
Eco47III AGCGCT 1 cut(s) 1090
Eco88I CYCGRG 3 cut(s) 215, 1003, 1301
EcoRI GAATTC 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 887, 967
EcoT14I CCWWGG 1 cut(s) 635
EcoT22I ATGCAT 4 cut(s) 459, 652, 1039, 1543
EcoT38I GRGCYC 2 cut(s) 345, 1524
ErhI CCWWGG 1 cut(s) 635
FauI CCCGC 2 cut(s) 825, 1679
FbaI TGATCA 1 cut(s) 1407
FblI GTMKAC 2 cut(s) 662, 1824
Fnu4HI GCNGC 9 cut(s) 261, 468, 720, 896, 1066, 1128, 1137, 1425, 1428
FokI GGATG 3 cut(s) 23, 1505, 1878
FriOI GRGCYC 2 cut(s) 345, 1524
Fsp4HI GCNGC 9 cut(s) 261, 468, 720, 896, 1066, 1128, 1137, 1425, 1428
FspBI CTAG 4 cut(s) 291, 435, 444, 1220
GlaI GCGC 2 cut(s) 1090, 1617
GluI GCNGC 9 cut(s) 261, 468, 720, 896, 1066, 1128, 1137, 1425, 1428
GsaI CCCAGC 1 cut(s) 735
GsuI CTGGAG 1 cut(s) 1884
HaeII RGCGCY 1 cut(s) 1092
HaeIII GGCC 3 cut(s) 390, 920, 1377
HapII CCGG 3 cut(s) 150, 1163, 1490
HgaI GACGC 1 cut(s) 47
HhaI GCGC 2 cut(s) 1091, 1618
Hin6I GCGC 2 cut(s) 1089, 1616
HinP1I GCGC 2 cut(s) 1089, 1616
HincII GTYRAC 1 cut(s) 1825
HindII GTYRAC 1 cut(s) 1825
HindIII AAGCTT 2 cut(s) 1059, 1739
HinfI GANTC 6 cut(s) 163, 844, 1001, 1401, 1694, 1817
HpaII CCGG 3 cut(s) 150, 1163, 1490
HphI GGTGA 3 cut(s) 89, 1479, 1720
Hpy166II GTNNAC 4 cut(s) 502, 663, 1528, 1825
Hpy188I TCNGA 7 cut(s) 91, 319, 533, 1098, 1278, 1586, 1612
Hpy188III TCNNGA 7 cut(s) 247, 685, 1082, 1388, 1405, 1445, 1901
Hpy8I GTNNAC 4 cut(s) 502, 663, 1528, 1825
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 5 cut(s) 155, 429, 707, 1366, 1684
HpyCH4III ACNGT 8 cut(s) 232, 313, 477, 596, 610, 1601, 1914, 1927
HpyCH4IV ACGT 1 cut(s) 1647
HpyF10VI GCNNNNNNNGC 9 cut(s) 728, 872, 1133, 1136, 1142, 1433, 1554, 1624, 1633
HpyF3I CTNAG 5 cut(s) 861, 993, 1248, 1443, 1728
HpySE526I ACGT 1 cut(s) 1647
HspAI GCGC 2 cut(s) 1089, 1616
Ksp22I TGATCA 1 cut(s) 1407
Kzo9I GATC 5 cut(s) 174, 700, 1254, 1407, 1766
LmnI GCTCC 5 cut(s) 716, 892, 911, 1096, 1903
Lsp1109I GCAGC 6 cut(s) 247, 454, 706, 907, 1114, 1414
LweI GCATC 8 cut(s) 352, 466, 659, 1046, 1132, 1351, 1483, 1879
MaeI CTAG 4 cut(s) 291, 435, 444, 1220
MaeII ACGT 1 cut(s) 1647
MaeIII GTNAC 5 cut(s) 604, 1319, 1708, 1779, 1921
MalI GATC 5 cut(s) 176, 702, 1256, 1409, 1768
MboI GATC 5 cut(s) 174, 700, 1254, 1407, 1766
MboII GAAGA 3 cut(s) 172, 961, 1010
MfeI CAATTG 3 cut(s) 555, 1041, 1501
MhlI GDGCHC 3 cut(s) 345, 1524, 1530
MlsI TGGCCA 1 cut(s) 390
MluNI TGGCCA 1 cut(s) 390
MlyI GAGTC 3 cut(s) 157, 995, 1703
MmeI TCCRAC 1 cut(s) 1722
Mox20I TGGCCA 1 cut(s) 390
Mph1103I ATGCAT 4 cut(s) 459, 652, 1039, 1543
MroXI GAANNNNTTC 1 cut(s) 1740
MscI TGGCCA 1 cut(s) 390
MseI TTAA 7 cut(s) 84, 108, 513, 828, 1359, 1718, 1930
MslI CAYNNNNRTG 3 cut(s) 475, 497, 708
Msp20I TGGCCA 1 cut(s) 390
MspI CCGG 3 cut(s) 150, 1163, 1490
MspR9I CCNGG 3 cut(s) 150, 889, 969
MunI CAATTG 3 cut(s) 555, 1041, 1501
Mva1269I GAATGC 1 cut(s) 1541
MvaI CCWGG 2 cut(s) 889, 969
MvnI CGCG 1 cut(s) 1686
MwoI GCNNNNNNNGC 9 cut(s) 728, 872, 1133, 1136, 1142, 1433, 1554, 1624, 1633
NciI CCSGG 1 cut(s) 150
NcoI CCATGG 1 cut(s) 635
NdeII GATC 5 cut(s) 174, 700, 1254, 1407, 1766
NlaIV GGNNCC 2 cut(s) 907, 1882
NmuCI GTSAC 2 cut(s) 604, 1708
NsiI ATGCAT 4 cut(s) 459, 652, 1039, 1543
NspI RCATGY 1 cut(s) 711
NspV TTCGAA 1 cut(s) 268
OliI CACNNNNGTG 2 cut(s) 475, 497
PaeR7I CTCGAG 1 cut(s) 1003
PagI TCATGA 2 cut(s) 246, 1404
PceI AGGCCT 1 cut(s) 920
PciI ACATGT 1 cut(s) 707
PctI GAATGC 1 cut(s) 1541
PdmI GAANNNNTTC 1 cut(s) 1740
PfeI GAWTC 3 cut(s) 844, 1401, 1817
PflFI GACNNNGTC 1 cut(s) 230
PflMI CCANNNNNTGG 1 cut(s) 1704
PkrI GCNGC 9 cut(s) 262, 469, 721, 897, 1067, 1129, 1138, 1426, 1429
PleI GAGTC 3 cut(s) 157, 995, 1702
PpsI GAGTC 3 cut(s) 157, 995, 1702
PscI ACATGT 1 cut(s) 707
Psp6I CCWGG 2 cut(s) 887, 967
PspFI CCCAGC 1 cut(s) 731
PspGI CCWGG 2 cut(s) 887, 967
PspN4I GGNNCC 2 cut(s) 907, 1882
PspPI GGNCC 2 cut(s) 502, 1375
PspXI VCTCGAGB 1 cut(s) 1003
PstNI CAGNNNCTG 2 cut(s) 230, 1145
PsyI GACNNNGTC 1 cut(s) 230
RsaI GTAC 4 cut(s) 26, 140, 1562, 1650
RsaNI GTAC 4 cut(s) 25, 139, 1561, 1649
RseI CAYNNNNRTG 3 cut(s) 475, 497, 708
SalI GTCGAC 1 cut(s) 1823
SaqAI TTAA 7 cut(s) 84, 108, 513, 828, 1359, 1718, 1930
SatI GCNGC 9 cut(s) 261, 468, 720, 896, 1066, 1128, 1137, 1425, 1428
Sau3AI GATC 5 cut(s) 174, 700, 1254, 1407, 1766
Sau96I GGNCC 2 cut(s) 502, 1375
ScaI AGTACT 1 cut(s) 140
SchI GAGTC 3 cut(s) 157, 995, 1703
ScrFI CCNGG 3 cut(s) 150, 889, 969
SduI GDGCHC 3 cut(s) 345, 1524, 1530
SfaNI GCATC 8 cut(s) 352, 466, 659, 1046, 1132, 1351, 1483, 1879
SfcI CTRYAG 2 cut(s) 813, 1597
Sfr274I CTCGAG 1 cut(s) 1003
SfuI TTCGAA 1 cut(s) 268
SinI GGWCC 1 cut(s) 502
SlaI CTCGAG 1 cut(s) 1003
SmiMI CAYNNNNRTG 3 cut(s) 475, 497, 708
SmlI CTYRAG 1 cut(s) 1003
SmoI CTYRAG 1 cut(s) 1003
SseBI AGGCCT 1 cut(s) 920
SsiI CCGC 6 cut(s) 832, 1066, 1137, 1424, 1548, 1686
SspI AATATT 1 cut(s) 1483
SspMI CTAG 4 cut(s) 291, 435, 444, 1220
StuI AGGCCT 1 cut(s) 920
StyD4I CCNGG 3 cut(s) 148, 887, 967
StyI CCWWGG 1 cut(s) 635
TaaI ACNGT 8 cut(s) 232, 313, 477, 596, 610, 1601, 1914, 1927
TaiI ACGT 1 cut(s) 1650
TaqI TCGA 8 cut(s) 234, 268, 1004, 1577, 1765, 1803, 1824, 1839
TatI WGTACW 1 cut(s) 138
TauI GCSGC 3 cut(s) 1068, 1139, 1427
TfiI GAWTC 3 cut(s) 844, 1401, 1817
Tru1I TTAA 7 cut(s) 84, 108, 513, 828, 1359, 1718, 1930
Tru9I TTAA 7 cut(s) 84, 108, 513, 828, 1359, 1718, 1930
TseFI GTSAC 2 cut(s) 604, 1708
TseI GCWGC 6 cut(s) 260, 467, 719, 895, 1127, 1427
Tsp45I GTSAC 2 cut(s) 604, 1708
TspDTI ATGAA 6 cut(s) 426, 707, 972, 1042, 1284, 1393
TspGWI ACGGA 1 cut(s) 713
Tth111I GACNNNGTC 1 cut(s) 230
Van91I CCANNNNNTGG 1 cut(s) 1704
VneI GTGCAC 1 cut(s) 1526
VpaK11BI GGWCC 1 cut(s) 502
XapI RAATTY 5 cut(s) 16, 271, 527, 786, 1291
XceI RCATGY 1 cut(s) 711
XcmI CCANNNNNNNNNTGG 1 cut(s) 496
XhoI CTCGAG 1 cut(s) 1003
XmiI GTMKAC 2 cut(s) 662, 1824
XmnI GAANNNNTTC 1 cut(s) 1740
XspI CTAG 4 cut(s) 291, 435, 444, 1220
ZrmI AGTACT 1 cut(s) 140
Zsp2I ATGCAT 4 cut(s) 459, 652, 1039, 1543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.