Rh4CG296600

plant mutator transposase zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000251
Physical Location & Seq
Reverse (-)
55582195 .. 55586527
4333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG296600.1

Sequence Viewer

Length: 2490 bp
ATGCTAGCGACGAAGATGACGACTCCTCCACCCTCCCTCTACTCCTCAAACGCCTCCCATGGACTTCGGCAGCGGCGCCTCCCTCAACTACTTCGACGACAAGGACGGCGATTTCTGCTCCGTGATCGTCAAGAGCTACCGCAATCGGACCACGAGGAGATTGACCAATCAAATTCATCTCAGTCTGGCTTCAAGGCCATGGCTGCAAAGAAAGTTATAGCAATATGTCAATCCGGAGGGGACTTCGTGACGGATAAGGATGGGAGTTTATCGTACAGTGGCGGAGACGCGTATGCAGTTGACATTGATCAGCAAACTCTGCTGAGTGATTTCAAGTCTGAAATAGCGGAGATGTTTAGCTGTAGTGCTGATACAATGTCGCTCAAGTATTTCCTTCCGGGCAATAAGAAGACGCTCATTACAATCTCTAAAGATAAGGACCTACAGCGGATGGTCAATTTCCTGGGGGATTCGGTCAGTGTGGATGTCTTTGTCATCTCGGAGGAAACTGCTGCTCGAAATACATCCAACATGCCTGCTAGTAGGTCAAGCCGGACAACTGTATCTGAAGCAGTAGTTCCTGTTGCTGAGCAGCTTGGCCTTGTTGATGTGCCAGTTGATACTAGCATTGCCATTGATCAAATTGATACAAAACCTCCACATGAAATTCCTATGTGTTCTGTTTTCCCAAGTTCTTCTCATGATGATAAGCATCATAAAGCTGCACAACAGTGGGAAAACACCATCACCGGAGTGGACCAACGGTTTAATAGTTTCAGTGAATTCAGAGAAGCATTGCATAAGTATTCAATTGCGCATGGGTTTGCTTATAGATATAAGAAAAATGACAGTCATCGAGTCACAGTGAAATGCAAATCCCAAGGTTGCCCATGGAGGATATATGCATCAAGGTTGGCCACTACACAGTTAATTTGTATCAAGAAAATGAACACAGATCATACATGTGAAGGGGCTGCAGTAAAAGCTGGGTATCGGGCAACTAGAGGTTGGGTGGGAAGTATCATAAAGGAGAAATTGAAAGTTTCCCCAAACTACAAGCCAAAGGATATAGCAGATGACATCAAGCGAGAGTATGGCATTCAGTTGAACTACTCTCAGGCATGGCGAGCGAAAGAGATTGCGAGGGAGCAGCTTCAAGGTTCTTATAAAGAGGCCTATAATCAGTTGCCTTATTTTTGTGAGAAGATAAAGGAAACTAATCCAGGGAGTGTTGCTACATTTGCCACTAAGGAGGACTCGAGCTTCCATCGCTTCTTTGTATCTTTCCATGCATCAATTTCAGGTTTCCAACAAGGTTGCCGCCCTCTCCTTTTCCTTGATAGCACTCCTTTGAACTCTAAATATCAAGGAGATTTGTTGTCTGCAACAGCTGCAGATGGTGATGATGGTATCTTTCCTGTAGCATTTGCTGTCGTGGATGCTGAGACTAGTGAAAACTGGCATTGGTTCTTATTAGAACTGAAATCTGCAGTGTCAACATCTCAGTCAATTACATTTGTTGCAGATTTTCAGAATGGTTTGAAAGAGTCATTGGCTGAGGTATTTGATAAATGCTACCACTGCTTTTGTTTGAGGCATCTTGCTGAGAAACTTAACAAGGACTTGAAGGGGCAATTTTCTCATGAGGCAAGACGGTTTTTAATTAATGATTTTTATTCTGCTGCTTATGCACCTAAACTGGAGGATTTCCAGCGTAGTGCTGGGAACATAAAAAGTATTTCACCTGATGCGTACGACTGGGTTGTACAAAGTGGACCAGAGCACTGGGCTAATGCATTCTCTTTAGGAGCCAGGTACAACCACATGACTTCAAACTTTGGACAGCAATTCTATAGTTGGGTATCAGAGGCACATGAGTTACCGATTACACAGATGATTGATGTGTTACGAGGTAAGATGATGGAAACCATATATTCTCGGAGGGTGGAGTCTAACCAATGGGTGACAAGATTAACGCCATCTAAGGAGGAAAAGCTTCAACAAGAAATGGAATCTGCTCGATCACTTCAAGTGTTACTCTCACACGGTAGCACATTTGAGGTTCGTGGAGACTCTGTTGATACTGTTGACATCGACCATTGGAATTGTTCCTGTAAGGGATGGCAACTTACTGGTCTGCCTTGTTGCCATGCTATTGCTGTCTTTGAATGCATTGGTAGGAGCTCATATGATTACTGCTCCAGATACTTCACCCTCGAGAGTTACCGTTTAACATATGCAGAATCAATCAACCCTGTTCCGAATGTAGACAGACCACTAGCAATCCCAGGCGGTGAATCAAGCCAGGCAGTAGCAGGAGTTACCGTTACCCCTCCACCTACCAAACGGCCGCCAGGGCGGCCAAAGCTGAAGTCAGCCGAAACAATAGACATAATAAAACGCCAGCTCCAGTGCAGCAAGTGCAAGGGCCTTGGCCACAATAAGAAGACATGTAAAGATCCTAATGTTATTTTAGAACTTCCGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE PB1 SWIM

Protein Analysis

829

Amino Acids

92.97

Weight (kDa)

7.94

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 87 - 156 5.1e-07 PB1 domain
DBD_Tnp_Mut PF03108 248 - 313 1.9e-25 MuDR family transposase
MULE PF10551 444 - 536 1.4e-15 MULE transposase domain
SWIM PF04434 695 - 725 7e-10 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06740
fragaria_vesca FvH4_1g02210 FvH4_1g02210 FvH4_1g02210 FvH4_1g02210 FvH4_2g02692 FvH4_2g22241 FvH4_2g22241 FvH4_2g22241 FvH4_4g21561 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g02501 FvH4_6g02501
malus_domestica MD02G1020500.v1.1 MD04G1227400.v1.1 MD04G1236400.v1.1 MD05G1203600.v1.1 MD10G1190900.v1.1 MD12G1244100.v1.1 MD12G1255700.v1.1 MD13G1140100.v1.1 MD15G1396600.v1.1 MD16G1135600.v1.1
prunus_persica Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.6G346500_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G229900_v2.0.a1 Prupe.8G229900_v2.0.a1
pyrus_communis pycom02g01800 pycom02g16220 pycom04g20090 pycom04g21020 pycom04g21030 pycom05g19010 pycom10g16460 pycom12g18690 pycom12g22380 pycom12g23430 pycom13g12180 pycom15g14690 pycom15g23420 pycom15g35670 pycom16g11960
rosa_chinensis RchiOBHm_Chr2g0087301 RchiOBHm_Chr3g0448611 RchiOBHm_Chr3g0450381 RchiOBHm_Chr4g0427621 RchiOBHm_Chr6g0247111 RchiOBHm_Chr6g0289171 RchiOBHm_Chr7g0177141
rosa_laevigata RLG00000007221 RLG00000015852
rosa_multiflora Rmu_co8326617.1_g000001 Rmu_sc0003399.1_g000010
rosa_roxburghii Rroxscaffold_2G00153730 Rroxscaffold_5G00369250 Rroxscaffold_6G00426900 Rroxscaffold_6G00428110 Rroxscaffold_7G00179510 Rroxscaffold_7G00214340
rosa_rugosa Rorug01G0473800 Rorug01G0473900 Rorug01G0474000 Rorug02G0614200 Rorug02G0614300 Rorug02G0614400 Rorug02G0614500 Rorug02G0614600 Rorug02G0627200 Rorug02G0627300 Rorug02G0627400 Rorug02G0627500 Rorug05G0522400
rosa_samantha Rh2AG027500 Rh2BG026800 Rh2CG027900 Rh2DG027500 Rh3AG013600 Rh3AG027900 Rh3BG013400 Rh3BG028400 Rh3CG012500 Rh3CG027200 Rh3DG014200 Rh3DG028300 Rh4AG275400 Rh4BG281300 Rh4CG296600 Rh4DG278600 Rh6AG037400 Rh6AG037700 Rh6AG312600 Rh6BG032300 Rh6BG032600 Rh6BG319500 Rh6CG033400 Rh6CG326000 Rh6DG031100 Rh6DG311900 Rh7AG003400 Rh7CG003000 Rh7CG003100
rosa_wichuraiana Rw2G002170 Rw3G001000 Rw3G002170 Rw4G023880 Rw6G003210 Rw6G027010 Rw7G000280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1167
Acc16I TGCGCA 1 cut(s) 816
AccB1I GGYRCC 1 cut(s) 75
AccI GTMKAC 1 cut(s) 2268
AccII CGCG 1 cut(s) 290
AccIII TCCGGA 1 cut(s) 233
AciI CCGC 9 cut(s) 73, 140, 282, 347, 448, 1321, 2292, 2351, 2359
AclWI GGATC 1 cut(s) 2453
AcoI YGGCCR 4 cut(s) 915, 2348, 2360, 2434
AcsI RAATTY 3 cut(s) 172, 666, 782
AcuI CTGAAG 2 cut(s) 588, 2390
AcyI GRCGYC 1 cut(s) 76
AfaI GTAC 4 cut(s) 275, 1754, 1767, 1817
AflIII ACRYGT 3 cut(s) 288, 962, 2450
AhlI ACTAGT 1 cut(s) 1448
AjnI CCWGG 6 cut(s) 462, 1222, 1811, 2287, 2304, 2353
AjuI GAANNNNNNNTTGG 2 cut(s) 1535, 1567
AleI CACNNNNGTG 2 cut(s) 730, 752
Alw21I GWGCWC 2 cut(s) 1785, 2186
Alw26I GTCTC 3 cut(s) 279, 1439, 2064
AlwI GGATC 1 cut(s) 2453
Ama87I CYCGRG 2 cut(s) 1258, 2216
Aor13HI TCCGGA 1 cut(s) 233
AoxI GGCC 8 cut(s) 195, 598, 915, 1173, 2348, 2360, 2428, 2434
ApoI RAATTY 3 cut(s) 172, 666, 782
ArsI GACNNNNNNTTYG 2 cut(s) 2357, 2389
AseI ATTAAT 1 cut(s) 1665
Asp700I GAANNNNTTC 1 cut(s) 1995
AspLEI GCGC 2 cut(s) 78, 817
AspS9I GGNCC 5 cut(s) 148, 439, 757, 1775, 2428
AsuC2I CCSGG 2 cut(s) 399, 2484
AsuHPI GGTGA 6 cut(s) 739, 1412, 1734, 1975, 2203, 2306
AsuNHI GCTAGC 1 cut(s) 4
AvaI CYCGRG 2 cut(s) 1258, 2216
AvaII GGWCC 4 cut(s) 148, 439, 757, 1775
BaeI ACNNNNGTAYC 2 cut(s) 546, 579
BalI TGGCCA 2 cut(s) 917, 2436
BanI GGYRCC 1 cut(s) 75
BanII GRGCYC 1 cut(s) 2186
BauI CACGAG 1 cut(s) 152
BbsI GAAGAC 2 cut(s) 416, 2453
Bbv12I GWGCWC 2 cut(s) 1785, 2186
BbvCI CCTCAGC 1 cut(s) 1557
BccI CCATC 9 cut(s) 254, 445, 752, 1275, 1391, 1400, 1915, 1987, 2115
BceAI ACGGC 2 cut(s) 122, 2363
BciT130I CCWGG 6 cut(s) 464, 1224, 1813, 2289, 2306, 2355
BclI TGATCA 2 cut(s) 307, 637
BcnI CCSGG 2 cut(s) 399, 2484
BcoDI GTCTC 3 cut(s) 279, 1439, 2064
BcuI ACTAGT 1 cut(s) 1448
BfaI CTAG 6 cut(s) 5, 540, 624, 1002, 1449, 2279
BfmI CTRYAG 7 cut(s) 361, 443, 975, 1392, 1419, 1488, 1852
BfoI RGCGCY 1 cut(s) 79
BglI GCCNNNNNGGC 2 cut(s) 2356, 2359
BlpI GCTNAGC 1 cut(s) 588
Bme1390I CCNGG 8 cut(s) 399, 464, 1224, 1813, 2289, 2306, 2355, 2484
Bme18I GGWCC 4 cut(s) 148, 439, 757, 1775
BmeT110I CYCGRG 2 cut(s) 1258, 2216
BmgT120I GGNCC 5 cut(s) 148, 439, 757, 1775, 2428
BmiI GGNNCC 2 cut(s) 77, 1810
BmrFI CCNGG 8 cut(s) 399, 464, 1224, 1813, 2289, 2306, 2355, 2484
BmrI ACTGGG 2 cut(s) 1768, 1795
BmsI GCATC 6 cut(s) 721, 914, 1301, 1429, 1606, 1738
BmtI GCTAGC 1 cut(s) 8
BmuI ACTGGG 2 cut(s) 1768, 1795
BpiI GAAGAC 2 cut(s) 416, 2453
BpmI CTGGAG 3 cut(s) 1721, 2185, 2393
Bpu10I CCTNAGC 1 cut(s) 1557
Bpu1102I GCTNAGC 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 2 cut(s) 399, 2484
BsaBI GATNNNNATC 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 76
BsaWI WCCGGW 2 cut(s) 233, 749
Bse1I ACTGG 7 cut(s) 614, 1463, 1704, 1763, 1790, 2137, 2410
Bse3DI GCAATG 2 cut(s) 627, 794
Bse8I GATNNNNATC 1 cut(s) 711
BseAI TCCGGA 1 cut(s) 233
BseBI CCWGG 6 cut(s) 464, 1224, 1813, 2289, 2306, 2355
BseGI GGATG 6 cut(s) 265, 456, 490, 524, 1444, 2126
BseJI GATNNNNATC 1 cut(s) 711
BseMI GCAATG 2 cut(s) 627, 794
BseMII CTCAG 8 cut(s) 194, 314, 579, 1130, 1434, 1517, 1548, 1596
BseNI ACTGG 7 cut(s) 614, 1463, 1704, 1763, 1790, 2137, 2410
BseRI GAGGAG 3 cut(s) 15, 34, 170
BseX3I CGGCCG 1 cut(s) 2348
BseYI CCCAGC 2 cut(s) 986, 1721
BsgI GTGCAG 2 cut(s) 708, 2434
Bsh1236I CGCG 1 cut(s) 290
Bsh1285I CGRYCG 1 cut(s) 2351
BshFI GGCC 8 cut(s) 197, 600, 917, 1175, 2350, 2362, 2430, 2436
BshNI GGYRCC 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 2351
BsiHKAI GWGCWC 2 cut(s) 1785, 2186
BsiHKCI CYCGRG 2 cut(s) 1258, 2216
BsiSI CCGG 5 cut(s) 234, 398, 553, 750, 2483
BsiWI CGTACG 1 cut(s) 1752
BslFI GGGAC 1 cut(s) 254
BsmAI GTCTC 3 cut(s) 279, 1439, 2064
BsmBI CGTCTC 1 cut(s) 279
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 3 cut(s) 1098, 1796, 2174
BsnI GGCC 8 cut(s) 197, 600, 917, 1175, 2350, 2362, 2430, 2436
BsoBI CYCGRG 2 cut(s) 1258, 2216
Bsp1286I GDGCHC 2 cut(s) 1785, 2186
Bsp13I TCCGGA 1 cut(s) 233
Bsp1407I TGTACA 1 cut(s) 1765
Bsp143I GATC 6 cut(s) 124, 307, 637, 955, 2021, 2458
Bsp1720I GCTNAGC 1 cut(s) 588
Bsp19I CCATGG 3 cut(s) 58, 198, 890
BspACI CCGC 9 cut(s) 73, 140, 282, 347, 448, 1321, 2292, 2351, 2359
BspANI GGCC 8 cut(s) 197, 600, 917, 1175, 2350, 2362, 2430, 2436
BspCNI CTCAG 8 cut(s) 193, 315, 580, 1129, 1435, 1516, 1549, 1597
BspEI TCCGGA 1 cut(s) 233
BspFNI CGCG 1 cut(s) 290
BspHI TCATGA 2 cut(s) 700, 1642
BspLI GGNNCC 2 cut(s) 77, 1810
BspMAI CTGCAG 3 cut(s) 979, 1396, 1492
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 1 cut(s) 2453
BspT107I GGYRCC 1 cut(s) 75
BsrDI GCAATG 2 cut(s) 627, 794
BsrGI TGTACA 1 cut(s) 1765
BsrI ACTGG 7 cut(s) 614, 1463, 1704, 1763, 1790, 2137, 2410
BssMI GATC 6 cut(s) 124, 307, 637, 955, 2021, 2458
BssNI GRCGYC 1 cut(s) 76
BssSI CACGAG 1 cut(s) 152
BssT1I CCWWGG 5 cut(s) 58, 198, 880, 890, 2431
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 6 cut(s) 464, 1224, 1813, 2289, 2306, 2355
BstACI GRCGYC 1 cut(s) 76
BstAPI GCANNNNNTGC 3 cut(s) 319, 1391, 2421
BstAUI TGTACA 1 cut(s) 1765
BstC8I GCNNGC 4 cut(s) 6, 537, 1128, 2405
BstDSI CCRYGG 3 cut(s) 58, 198, 890
BstF5I GGATG 6 cut(s) 265, 456, 490, 524, 1444, 2126
BstFNI CGCG 1 cut(s) 290
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 2 cut(s) 78, 817
BstKTI GATC 6 cut(s) 127, 310, 640, 958, 2024, 2461
BstMAI GTCTC 3 cut(s) 279, 1439, 2064
BstMBI GATC 6 cut(s) 124, 307, 637, 955, 2021, 2458
BstMCI CGRYCG 1 cut(s) 2351
BstNI CCWGG 6 cut(s) 464, 1224, 1813, 2289, 2306, 2355
BstNSI RCATGY 3 cut(s) 535, 966, 2454
BstSCI CCNGG 8 cut(s) 397, 462, 1222, 1811, 2287, 2304, 2353, 2482
BstSFI CTRYAG 7 cut(s) 361, 443, 975, 1392, 1419, 1488, 1852
BstUI CGCG 1 cut(s) 290
BstV2I GAAGAC 2 cut(s) 416, 2453
BstX2I RGATCY 1 cut(s) 2458
BstXI CCANNNNNNTGG 1 cut(s) 1785
BstYI RGATCY 1 cut(s) 2458
BstZI CGGCCG 1 cut(s) 2348
BsuRI GGCC 8 cut(s) 197, 600, 917, 1175, 2350, 2362, 2430, 2436
BtgI CCRYGG 3 cut(s) 58, 198, 890
BtgZI GCGATG 1 cut(s) 1253
BtsCI GGATG 6 cut(s) 265, 456, 490, 524, 1444, 2126
BtsI GCAGTG 2 cut(s) 1497, 1579
BtsIMutI CAGTG 9 cut(s) 283, 484, 737, 784, 870, 1497, 1579, 1783, 2417
Cac8I GCNNGC 4 cut(s) 6, 537, 1128, 2405
CciI TCATGA 2 cut(s) 700, 1642
CfoI GCGC 2 cut(s) 78, 817
Cfr13I GGNCC 5 cut(s) 148, 439, 757, 1775, 2428
CseI GACGC 2 cut(s) 296, 421
Csp6I GTAC 4 cut(s) 274, 1753, 1766, 1816
CspCI CAANNNNNGTGG 2 cut(s) 1568, 1603
CviQI GTAC 4 cut(s) 274, 1753, 1766, 1816
DinI GGCGCC 1 cut(s) 77
DpnI GATC 6 cut(s) 126, 309, 639, 957, 2023, 2460
DpnII GATC 6 cut(s) 124, 307, 637, 955, 2021, 2458
EaeI YGGCCR 4 cut(s) 915, 2348, 2360, 2434
EagI CGGCCG 1 cut(s) 2348
EciI GGCGGA 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 2184
EclXI CGGCCG 1 cut(s) 2348
Eco130I CCWWGG 5 cut(s) 58, 198, 880, 890, 2431
Eco147I AGGCCT 1 cut(s) 1175
Eco24I GRGCYC 1 cut(s) 2186
Eco47I GGWCC 4 cut(s) 148, 439, 757, 1775
Eco52I CGGCCG 1 cut(s) 2348
Eco53kI GAGCTC 1 cut(s) 2184
Eco57I CTGAAG 2 cut(s) 588, 2390
Eco88I CYCGRG 2 cut(s) 1258, 2216
EcoICRI GAGCTC 1 cut(s) 2184
EcoO109I RGGNCCY 2 cut(s) 439, 2428
EcoRI GAATTC 1 cut(s) 782
EcoRII CCWGG 6 cut(s) 462, 1222, 1811, 2287, 2304, 2353
EcoT14I CCWWGG 5 cut(s) 58, 198, 880, 890, 2431
EcoT22I ATGCAT 4 cut(s) 907, 1294, 1798, 2174
EcoT38I GRGCYC 1 cut(s) 2186
EgeI GGCGCC 1 cut(s) 77
EheI GGCGCC 1 cut(s) 77
ErhI CCWWGG 5 cut(s) 58, 198, 880, 890, 2431
Esp3I CGTCTC 1 cut(s) 279
FaqI GGGAC 1 cut(s) 254
FauNDI CATATG 2 cut(s) 2188, 2236
FbaI TGATCA 2 cut(s) 307, 637
FblI GTMKAC 1 cut(s) 2268
FokI GGATG 6 cut(s) 272, 463, 497, 511, 1451, 2133
FriOI GRGCYC 1 cut(s) 2186
FspBI CTAG 6 cut(s) 5, 540, 624, 1002, 1449, 2279
FspI TGCGCA 1 cut(s) 816
GlaI GCGC 2 cut(s) 77, 816
GsaI CCCAGC 2 cut(s) 990, 1725
GsuI CTGGAG 3 cut(s) 1721, 2185, 2393
HaeII RGCGCY 1 cut(s) 79
HaeIII GGCC 8 cut(s) 197, 600, 917, 1175, 2350, 2362, 2430, 2436
HapII CCGG 5 cut(s) 234, 398, 553, 750, 2483
HgaI GACGC 2 cut(s) 296, 421
HhaI GCGC 2 cut(s) 78, 817
Hin1I GRCGYC 1 cut(s) 76
Hin6I GCGC 2 cut(s) 76, 815
HinP1I GCGC 2 cut(s) 76, 815
HincII GTYRAC 3 cut(s) 301, 1497, 2089
HindII GTYRAC 3 cut(s) 301, 1497, 2089
HindIII AAGCTT 1 cut(s) 1994
HpaII CCGG 5 cut(s) 234, 398, 553, 750, 2483
HphI GGTGA 6 cut(s) 739, 1412, 1734, 1975, 2203, 2306
Hpy166II GTNNAC 6 cut(s) 301, 757, 1497, 1775, 2089, 2269
Hpy188I TCNGA 9 cut(s) 148, 340, 502, 568, 788, 1533, 1867, 1941, 2262
Hpy188III TCNNGA 8 cut(s) 131, 234, 247, 701, 940, 1643, 2202, 2218
Hpy8I GTNNAC 6 cut(s) 301, 757, 1497, 1775, 2089, 2269
Hpy99I CGWCG 2 cut(s) 13, 99
HpyAV CCTTC 3 cut(s) 404, 962, 1621
Hsp92I GRCGYC 1 cut(s) 76
HspAI GCGC 2 cut(s) 76, 815
KasI GGCGCC 1 cut(s) 75
Kpn2I TCCGGA 1 cut(s) 233
Ksp22I TGATCA 2 cut(s) 307, 637
Kzo9I GATC 6 cut(s) 124, 307, 637, 955, 2021, 2458
LmnI GCTCC 6 cut(s) 123, 1147, 1808, 2181, 2204, 2412
LweI GCATC 6 cut(s) 721, 914, 1301, 1429, 1606, 1738
MaeI CTAG 6 cut(s) 5, 540, 624, 1002, 1449, 2279
MaeIII GTNAC 9 cut(s) 247, 859, 1878, 1905, 1963, 2034, 2222, 2320, 2326
MalI GATC 6 cut(s) 126, 309, 639, 957, 2023, 2460
MboI GATC 6 cut(s) 124, 307, 637, 955, 2021, 2458
MboII GAAGA 5 cut(s) 25, 421, 687, 1216, 2458
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 1 cut(s) 2458
MhlI GDGCHC 2 cut(s) 1785, 2186
MlsI TGGCCA 2 cut(s) 917, 2436
MluI ACGCGT 1 cut(s) 288
MluNI TGGCCA 2 cut(s) 917, 2436
Mly113I GGCGCC 1 cut(s) 76
MlyI GAGTC 6 cut(s) 16, 867, 1250, 1556, 1958, 2066
MmeI TCCRAC 2 cut(s) 552, 1333
Mox20I TGGCCA 2 cut(s) 917, 2436
Mph1103I ATGCAT 4 cut(s) 907, 1294, 1798, 2174
MroI TCCGGA 1 cut(s) 233
MroXI GAANNNNTTC 1 cut(s) 1995
MscI TGGCCA 2 cut(s) 917, 2436
MseI TTAA 7 cut(s) 768, 929, 1614, 1661, 1665, 1973, 2231
MslI CAYNNNNRTG 3 cut(s) 730, 752, 963
Msp20I TGGCCA 2 cut(s) 917, 2436
MspA1I CMGCKG 3 cut(s) 73, 448, 1391
MspI CCGG 5 cut(s) 234, 398, 553, 750, 2483
MspR9I CCNGG 8 cut(s) 399, 464, 1224, 1813, 2289, 2306, 2355, 2484
MunI CAATTG 1 cut(s) 810
Mva1269I GAATGC 3 cut(s) 1098, 1796, 2174
MvaI CCWGG 6 cut(s) 464, 1224, 1813, 2289, 2306, 2355
MvnI CGCG 1 cut(s) 290
NarI GGCGCC 1 cut(s) 76
NciI CCSGG 2 cut(s) 399, 2484
NcoI CCATGG 3 cut(s) 58, 198, 890
NdeI CATATG 2 cut(s) 2188, 2236
NdeII GATC 6 cut(s) 124, 307, 637, 955, 2021, 2458
NheI GCTAGC 1 cut(s) 4
NlaIV GGNNCC 2 cut(s) 77, 1810
NmuCI GTSAC 3 cut(s) 247, 859, 1963
NsbI TGCGCA 1 cut(s) 816
NsiI ATGCAT 4 cut(s) 907, 1294, 1798, 2174
NspI RCATGY 3 cut(s) 535, 966, 2454
OliI CACNNNNGTG 2 cut(s) 730, 752
PacI TTAATTAA 1 cut(s) 1665
PaeR7I CTCGAG 2 cut(s) 1258, 2216
PagI TCATGA 2 cut(s) 700, 1642
PceI AGGCCT 1 cut(s) 1175
PciI ACATGT 2 cut(s) 962, 2450
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PctI GAATGC 3 cut(s) 1098, 1796, 2174
PdmI GAANNNNTTC 1 cut(s) 1995
PfeI GAWTC 4 cut(s) 470, 2012, 2243, 2297
Pfl23II CGTACG 1 cut(s) 1752
PleI GAGTC 6 cut(s) 16, 866, 1250, 1555, 1957, 2066
PluTI GGCGCC 1 cut(s) 79
PpsI GAGTC 6 cut(s) 16, 866, 1250, 1555, 1957, 2066
PpuMI RGGWCCY 1 cut(s) 439
PscI ACATGT 2 cut(s) 962, 2450
PshBI ATTAAT 1 cut(s) 1665
PsiI TTATAA 1 cut(s) 1167
Psp124BI GAGCTC 1 cut(s) 2186
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 6 cut(s) 462, 1222, 1811, 2287, 2304, 2353
PspFI CCCAGC 2 cut(s) 986, 1721
PspGI CCWGG 6 cut(s) 462, 1222, 1811, 2287, 2304, 2353
PspLI CGTACG 1 cut(s) 1752
PspN4I GGNNCC 2 cut(s) 77, 1810
PspPI GGNCC 5 cut(s) 148, 439, 757, 1775, 2428
PspPPI RGGWCCY 1 cut(s) 439
PspXI VCTCGAGB 1 cut(s) 1258
PstI CTGCAG 3 cut(s) 979, 1396, 1492
PsuI RGATCY 1 cut(s) 2458
PvuII CAGCTG 1 cut(s) 1391
RsaI GTAC 4 cut(s) 275, 1754, 1767, 1817
RsaNI GTAC 4 cut(s) 274, 1753, 1766, 1816
RseI CAYNNNNRTG 3 cut(s) 730, 752, 963
SacI GAGCTC 1 cut(s) 2186
SaqAI TTAA 7 cut(s) 768, 929, 1614, 1661, 1665, 1973, 2231
Sau3AI GATC 6 cut(s) 124, 307, 637, 955, 2021, 2458
Sau96I GGNCC 5 cut(s) 148, 439, 757, 1775, 2428
SchI GAGTC 6 cut(s) 16, 867, 1250, 1556, 1958, 2066
ScrFI CCNGG 8 cut(s) 399, 464, 1224, 1813, 2289, 2306, 2355, 2484
SduI GDGCHC 2 cut(s) 1785, 2186
SfaNI GCATC 6 cut(s) 721, 914, 1301, 1429, 1606, 1738
SfcI CTRYAG 7 cut(s) 361, 443, 975, 1392, 1419, 1488, 1852
SfoI GGCGCC 1 cut(s) 77
Sfr274I CTCGAG 2 cut(s) 1258, 2216
SinI GGWCC 4 cut(s) 148, 439, 757, 1775
SlaI CTCGAG 2 cut(s) 1258, 2216
SmiMI CAYNNNNRTG 3 cut(s) 730, 752, 963
SmlI CTYRAG 3 cut(s) 383, 1258, 2216
SmoI CTYRAG 3 cut(s) 383, 1258, 2216
SpeI ACTAGT 1 cut(s) 1448
SseBI AGGCCT 1 cut(s) 1175
SsiI CCGC 9 cut(s) 73, 140, 282, 347, 448, 1321, 2292, 2351, 2359
SspDI GGCGCC 1 cut(s) 75
SspMI CTAG 6 cut(s) 5, 540, 624, 1002, 1449, 2279
SstI GAGCTC 1 cut(s) 2186
StuI AGGCCT 1 cut(s) 1175
StyD4I CCNGG 8 cut(s) 397, 462, 1222, 1811, 2287, 2304, 2353, 2482
StyI CCWWGG 5 cut(s) 58, 198, 880, 890, 2431
TaqI TCGA 7 cut(s) 94, 517, 856, 1259, 2020, 2094, 2217
TaqII GACCGA 1 cut(s) 463
TatI WGTACW 1 cut(s) 1765
TauI GCSGC 4 cut(s) 76, 1323, 2353, 2362
TfiI GAWTC 4 cut(s) 470, 2012, 2243, 2297
Tru1I TTAA 7 cut(s) 768, 929, 1614, 1661, 1665, 1973, 2231
Tru9I TTAA 7 cut(s) 768, 929, 1614, 1661, 1665, 1973, 2231
TscAI CASTG 9 cut(s) 283, 484, 737, 784, 870, 1497, 1586, 1790, 2417
TseFI GTSAC 3 cut(s) 247, 859, 1963
Tsp45I GTSAC 3 cut(s) 247, 859, 1963
TspDTI ATGAA 3 cut(s) 165, 678, 962
TspGWI ACGGA 2 cut(s) 110, 266
TspRI CASTG 9 cut(s) 283, 484, 737, 784, 870, 1497, 1586, 1790, 2417
VpaK11BI GGWCC 4 cut(s) 148, 439, 757, 1775
VspI ATTAAT 1 cut(s) 1665
XapI RAATTY 3 cut(s) 172, 666, 782
XceI RCATGY 3 cut(s) 535, 966, 2454
XcmI CCANNNNNNNNNTGG 2 cut(s) 751, 1718
XhoI CTCGAG 2 cut(s) 1258, 2216
XmiI GTMKAC 1 cut(s) 2268
XmnI GAANNNNTTC 1 cut(s) 1995
XspI CTAG 6 cut(s) 5, 540, 624, 1002, 1449, 2279
Zsp2I ATGCAT 4 cut(s) 907, 1294, 1798, 2174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.