MD16G1090300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000475
Physical Location & Seq
Forward (+)
6289195 .. 6290067
873 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1090300.v1.1.491

Sequence Viewer

Length: 873 bp
ATGGAGATCCCAAGATTTTCCGAGATGAAGCTGTGGTTTCGTGACACCATTGGCGTGATCGGATCAGCCGCGAACATCCCATTTCGAAATCCCAGTTTCATAACATTCGTACTGATTACCTCCTTTCCACTATTTTGTATGACTTTAGTACCAAGATTGCCTTCTAGCCAGCCTCCTTTGATCATGGAGGAGGCGGCAATTCAGCTAAGAGTTCTTACTCACCACCCCAAAGGTCACATGATTCAACTTCTGAGAGTGTACCTTTCTGAACTTGTTAGTTTCTTCACTGCACTCACAACTATATATGCTGCTTCAACAGTCTACATTAGTAGTGATAGGTGGAAAGTGAGTCTCCGAGATCTTCTTCGAAACTCAATAACCAAAACAAGGTGGCGCGAGCCCATGTTTACATTCTTGACTGTGTCCCTCTTGTCACACATTTGTTTAAGTTCTGTTTACTATTGGCATCATCACGCGAAGCCATTGCTTTCGTCAAGCTGCTCAAACAGGTTCTTGCAGGCTATAAACATGGTGGTTCCTGTTGTGGCCTTTGATAAATGGGTGGAGTACGGCGCTTGGTGGAATCTGAGTGTTGTGGTTTCCATTTTAGAGAAGGATAACAGAGGTTTCGAAGCCTTTTCAGATGCAGCGGAGTTGAGCGAAGGAAATACAAGAAGAGGGTTCGTTTTGATGCTTTTGTATTGTGTTTGGAGCTCCCGTTTTCCGACCCTCATTGCAAAATGCACTTTTCCAAGTGTAATTGCATATGATGTTCTTGACACAAGCTTCCTGTGCTTGGGGAAGGTTATGAATTGGGTGGTTCTCACGGTTTATTACTACGATTGTAAGAACCGTCACAAAGGGTTGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

291

Amino Acids

33.37

Weight (kDa)

9.17

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000475)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23830 AT1G23840 AT1G23850
fragaria_vesca FvH4_3g33230 FvH4_4g34710 FvH4_4g34711 FvH4_4g34720 FvH4_4g34730 FvH4_4g34740 FvH4_4g34760 FvH4_4g34761 FvH4_6g20500 FvH4_6g20520
malus_domestica MD13G1090000.v1.1 MD16G1090300.v1.1 MD16G1090400.v1.1 MD16G1090500.v1.1 MD16G1090600.v1.1
prunus_persica Prupe.1G255400_v2.0.a1 Prupe.1G255600_v2.0.a1 Prupe.1G255700_v2.0.a1 Prupe.1G255800_v2.0.a1
pyrus_communis pycom16g07740 pycom16g07760
rosa_chinensis RchiOBHm_Chr3g0476381 RchiOBHm_Chr4g0444081 RchiOBHm_Chr4g0444091 RchiOBHm_Chr4g0444101
rosa_laevigata RLG00000005856 RLG00000005857 RLG00000005858 RLG00000005859 RLG00000005860 RLG00000023773 RLG00000023775
rosa_multiflora Rmu_co8429785.1_g000001 Rmu_sc0000114.1_g000007 Rmu_sc0002177.1_g000009 Rmu_sc0012638.1_g000002 Rmu_sc0012638.1_g000003
rosa_roxburghii Rroxscaffold_5G00384630 Rroxscaffold_5G00384650 Rroxscaffold_5G00384660 Rroxscaffold_5G00384670 Rroxscaffold_5G00384680 Rroxscaffold_5G00384690 Rroxscaffold_6G00405320 Rroxscaffold_6G00405350
rosa_rugosa Rorug03G0153900 Rorug03G0154000 Rorug04G0349500 Rorug04G0349500 Rorug04G0349500
rosa_samantha Rh3AG205100 Rh3BG236700 Rh3CG231200 Rh3DG231000 Rh4AG410200 Rh4AG410300 Rh4AG410400 Rh4AG410500 Rh4AG410600 Rh4BG421300 Rh4BG421400 Rh4BG421500 Rh4BG421600 Rh4BG421700 Rh4BG421800 Rh4BG421900 Rh4BG422000 Rh4CG435700 Rh4CG435800 Rh4CG435900 Rh4CG436000 Rh4CG436100 Rh4CG436200 Rh4CG436300 Rh4CG436400 Rh4DG416600 Rh4DG416800 Rh4DG416900 Rh4DG417000 Rh4DG417100 Rh4DG417200 Rh4DG417300
rosa_wichuraiana Rw3G018640 Rw4G035240 Rw4G035250 Rw4G035260 Rw4G035270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 321
AccII CGCG 3 cut(s) 71, 396, 476
AciI CCGC 3 cut(s) 69, 194, 650
AclWI GGATC 1 cut(s) 70
AfaI GTAC 4 cut(s) 111, 150, 260, 569
AfiI CCNNNNNNNGG 2 cut(s) 387, 796
AgsI TTSAA 2 cut(s) 245, 315
AjuI GAANNNNNNNTTGG 2 cut(s) 145, 177
AluBI AGCT 5 cut(s) 31, 205, 498, 714, 786
AluI AGCT 5 cut(s) 31, 205, 498, 714, 786
Alw21I GWGCWC 1 cut(s) 716
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 1 cut(s) 70
AoxI GGCC 1 cut(s) 546
ApeKI GCWGC 3 cut(s) 308, 498, 647
AspLEI GCGC 2 cut(s) 396, 575
AsuHPI GGTGA 1 cut(s) 212
AsuII TTCGAA 3 cut(s) 85, 367, 630
BaeI ACNNNNGTAYC 2 cut(s) 132, 165
BanII GRGCYC 2 cut(s) 402, 716
Bbv12I GWGCWC 1 cut(s) 716
BbvI GCAGC 3 cut(s) 295, 485, 659
BceAI ACGGC 1 cut(s) 586
BcgI CGANNNNNNTGC 2 cut(s) 466, 500
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 576
BglII AGATCT 1 cut(s) 358
BisI GCNGC 5 cut(s) 69, 195, 309, 499, 648
BlsI GCNGC 5 cut(s) 70, 196, 310, 500, 649
BmiI GGNNCC 1 cut(s) 537
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 3 cut(s) 475, 634, 681
BmuI ACTGGG 1 cut(s) 87
Bpu14I TTCGAA 3 cut(s) 85, 367, 630
BsaXI ACNNNNNCTCC 2 cut(s) 703, 733
Bsc4I CCNNNNNNNGG 2 cut(s) 387, 796
Bse1I ACTGG 1 cut(s) 93
Bse3DI GCAATG 2 cut(s) 482, 732
BseGI GGATG 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 387, 796
BseMI GCAATG 2 cut(s) 482, 732
BseMII CTCAG 2 cut(s) 242, 578
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 203
BseXI GCAGC 3 cut(s) 295, 485, 659
BsgI GTGCAG 1 cut(s) 273
Bsh1236I CGCG 3 cut(s) 71, 396, 476
BshFI GGCC 1 cut(s) 548
BsiHKAI GWGCWC 1 cut(s) 716
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 2 cut(s) 387, 796
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 409
BsnI GGCC 1 cut(s) 548
Bsp119I TTCGAA 3 cut(s) 85, 367, 630
Bsp1286I GDGCHC 2 cut(s) 402, 716
Bsp143I GATC 5 cut(s) 6, 57, 62, 180, 358
BspACI CCGC 3 cut(s) 69, 194, 650
BspANI GGCC 1 cut(s) 548
BspCNI CTCAG 2 cut(s) 243, 579
BspFNI CGCG 3 cut(s) 71, 396, 476
BspLI GGNNCC 1 cut(s) 537
BspPI GGATC 1 cut(s) 70
BspT104I TTCGAA 3 cut(s) 85, 367, 630
BsrDI GCAATG 2 cut(s) 482, 732
BsrI ACTGG 1 cut(s) 93
BssMI GATC 5 cut(s) 6, 57, 62, 180, 358
Bst4CI ACNGT 4 cut(s) 319, 421, 829, 854
Bst6I CTCTTC 1 cut(s) 670
BstBI TTCGAA 3 cut(s) 85, 367, 630
BstC8I GCNNGC 3 cut(s) 170, 398, 519
BstDEI CTNAG 3 cut(s) 206, 251, 587
BstF5I GGATG 1 cut(s) 75
BstFNI CGCG 3 cut(s) 71, 396, 476
BstH2I RGCGCY 1 cut(s) 576
BstHHI GCGC 2 cut(s) 396, 575
BstKTI GATC 5 cut(s) 9, 60, 65, 183, 361
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 5 cut(s) 6, 57, 62, 180, 358
BstMWI GCNNNNNNNGC 1 cut(s) 792
BstUI CGCG 3 cut(s) 71, 396, 476
BstV1I GCAGC 3 cut(s) 295, 485, 659
BstX2I RGATCY 2 cut(s) 6, 358
BstYI RGATCY 2 cut(s) 6, 358
BsuRI GGCC 1 cut(s) 548
BtsCI GGATG 1 cut(s) 75
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 3 cut(s) 170, 398, 519
CfoI GCGC 2 cut(s) 396, 575
Csp6I GTAC 4 cut(s) 110, 149, 259, 568
CviAII CATG 4 cut(s) 184, 238, 403, 529
CviQI GTAC 4 cut(s) 110, 149, 259, 568
DdeI CTNAG 3 cut(s) 206, 251, 587
DpnI GATC 5 cut(s) 8, 59, 64, 182, 360
DpnII GATC 5 cut(s) 6, 57, 62, 180, 358
Eam1104I CTCTTC 1 cut(s) 670
EarI CTCTTC 1 cut(s) 670
Ecl136II GAGCTC 1 cut(s) 714
Eco24I GRGCYC 2 cut(s) 402, 716
Eco53kI GAGCTC 1 cut(s) 714
EcoICRI GAGCTC 1 cut(s) 714
EcoT38I GRGCYC 2 cut(s) 402, 716
FaeI CATG 4 cut(s) 187, 241, 406, 532
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FaqI GGGAC 1 cut(s) 409
FatI CATG 4 cut(s) 183, 237, 402, 528
FauNDI CATATG 1 cut(s) 766
FbaI TGATCA 1 cut(s) 180
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 5 cut(s) 69, 195, 309, 499, 648
FokI GGATG 1 cut(s) 62
FriOI GRGCYC 2 cut(s) 402, 716
Fsp4HI GCNGC 5 cut(s) 69, 195, 309, 499, 648
FspBI CTAG 1 cut(s) 165
GlaI GCGC 2 cut(s) 395, 574
GluI GCNGC 5 cut(s) 69, 195, 309, 499, 648
HaeII RGCGCY 1 cut(s) 576
HaeIII GGCC 1 cut(s) 548
HhaI GCGC 2 cut(s) 396, 575
Hin1II CATG 4 cut(s) 187, 241, 406, 532
Hin6I GCGC 2 cut(s) 394, 573
HinP1I GCGC 2 cut(s) 394, 573
HindIII AAGCTT 1 cut(s) 784
HinfI GANTC 3 cut(s) 241, 349, 583
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 4 cut(s) 259, 322, 408, 457
Hpy188I TCNGA 8 cut(s) 22, 62, 252, 268, 356, 588, 643, 726
Hpy188III TCNNGA 3 cut(s) 41, 415, 776
Hpy8I GTNNAC 4 cut(s) 259, 322, 408, 457
HpyAV CCTTC 4 cut(s) 171, 607, 656, 796
HpyCH4III ACNGT 4 cut(s) 319, 421, 829, 854
HpyCH4V TGCA 6 cut(s) 290, 517, 647, 737, 744, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 792
HpyF3I CTNAG 3 cut(s) 206, 251, 587
Hsp92II CATG 4 cut(s) 187, 241, 406, 532
HspAI GCGC 2 cut(s) 394, 573
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 5 cut(s) 6, 57, 62, 180, 358
LmnI GCTCC 2 cut(s) 711, 719
LpnPI CCDG 6 cut(s) 106, 182, 493, 503, 552, 803
Lsp1109I GCAGC 3 cut(s) 295, 485, 659
LweI GCATC 3 cut(s) 475, 634, 681
MaeI CTAG 1 cut(s) 165
MaeIII GTNAC 4 cut(s) 41, 233, 432, 854
MalI GATC 5 cut(s) 8, 59, 64, 182, 360
MboI GATC 5 cut(s) 6, 57, 62, 180, 358
MboII GAAGA 4 cut(s) 274, 353, 356, 687
MflI RGATCY 2 cut(s) 6, 358
MhlI GDGCHC 2 cut(s) 402, 716
MluCI AATT 3 cut(s) 198, 759, 811
MlyI GAGTC 1 cut(s) 358
MmeI TCCRAC 1 cut(s) 749
MnlI CCTC 8 cut(s) 130, 181, 183, 184, 437, 617, 671, 740
MseI TTAA 1 cut(s) 446
MslI CAYNNNNRTG 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 650
MvnI CGCG 3 cut(s) 71, 396, 476
MwoI GCNNNNNNNGC 1 cut(s) 792
NdeI CATATG 1 cut(s) 766
NdeII GATC 5 cut(s) 6, 57, 62, 180, 358
NlaIII CATG 4 cut(s) 187, 241, 406, 532
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 4 cut(s) 41, 233, 432, 854
NspV TTCGAA 3 cut(s) 85, 367, 630
PfeI GAWTC 2 cut(s) 241, 583
PflFI GACNNNGTC 1 cut(s) 421
PkrI GCNGC 5 cut(s) 70, 196, 310, 500, 649
PleI GAGTC 1 cut(s) 357
PpsI GAGTC 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 716
PspN4I GGNNCC 1 cut(s) 537
PsuI RGATCY 2 cut(s) 6, 358
PsyI GACNNNGTC 1 cut(s) 421
RsaI GTAC 4 cut(s) 111, 150, 260, 569
RsaNI GTAC 4 cut(s) 110, 149, 259, 568
RseI CAYNNNNRTG 1 cut(s) 53
SacI GAGCTC 1 cut(s) 716
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 5 cut(s) 69, 195, 309, 499, 648
Sau3AI GATC 5 cut(s) 6, 57, 62, 180, 358
SchI GAGTC 1 cut(s) 358
SduI GDGCHC 2 cut(s) 402, 716
SfaNI GCATC 3 cut(s) 475, 634, 681
SfuI TTCGAA 3 cut(s) 85, 367, 630
SmiMI CAYNNNNRTG 1 cut(s) 53
Sse9I AATT 3 cut(s) 198, 759, 811
SsiI CCGC 3 cut(s) 69, 194, 650
SspMI CTAG 1 cut(s) 165
SstI GAGCTC 1 cut(s) 716
TaaI ACNGT 4 cut(s) 319, 421, 829, 854
TaqI TCGA 3 cut(s) 85, 367, 630
TasI AATT 3 cut(s) 198, 759, 811
TauI GCSGC 2 cut(s) 71, 197
TfiI GAWTC 2 cut(s) 241, 583
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 4 cut(s) 41, 233, 432, 854
TseI GCWGC 3 cut(s) 308, 498, 647
Tsp45I GTSAC 4 cut(s) 41, 233, 432, 854
TspDTI ATGAA 3 cut(s) 41, 88, 824
TspRI CASTG 1 cut(s) 292
Tth111I GACNNNGTC 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.