MD16G1090400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000475
Physical Location & Seq
Forward (+)
6291028 .. 6291969
942 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1090400.v1.1.491

Sequence Viewer

Length: 942 bp
ATGAAGATCCCGAGATTTTCTGAGGTGATGCAATGGATTCTCGGCACGTTTGGTATCATTGAATCTGCCCTGACAATCCCATTTCGAAATCCCAATTTCATAATCTTGACATTGATTACTTCCCTTCCACTATTTTTTCTGACCTTGATACACCAATTGCCTTATGACTTCCAGCCTAGTTTGATTGTGCACGCAATAAATCTGCGAACGAGTCTTTCTTACCACTCCAACCCTCTGATTTCTGATATTTCATGGGCTCTAGATGTAGCCATTCAAATTCTGCAAGTGTATTTTTTTACCCTCGGAAACTTCCTCATTGTACTCACAACCATCCATGCTGCCTCAACAATCTATGCTAGCAATGAGAGGTCAATGATTGGTCTGCGAAATCTTCTTCGAAGTTCAGTGATGAAAAAAAGGTGGCACGAGCGTATGCTTACATTCTTCTCTATTTCCCTTCTGTGCAATCTTTCTTCGGCTGCTGTTGTCTATTGGCATTACGCAAGGCCGTTATACCCGATAGGCTGCCCTACTAGGTTCTTCCGGCTCTTCCGGAAGATACATTGGATAGTTTACGGCCTGGCCTTTGCGATATGGTTAGATTACAGCGCACGGTGGAACTTAAGCGTTGTTGTTTCCATTTTAGAGGAGAAGGTAGGAGTATTTGAAGCCATTTCAGCCTCATCAGAGCTAACCAAAGGAAACAGACTAAGAGGCTTCTTTCTTATGCTTCTGTTTTCTGTTGCAAAGTTCAATTTGCCAGCCCTCGTTGCTCGGGAATTTACAACTATCTTTGCTTTTTATTTCCTTGACACAATCTTCATGTGCTTGGGGAATGTTATCATTTGGGAGGTTCTCACAGTTTATTACTACGATTGTAAGAACCGTCACCAAAAGGCCGCGGCCGTGACTTCTTTTGATACAGGCAATACTCTACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

314

Amino Acids

36.13

Weight (kDa)

9.56

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000475)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23830 AT1G23840 AT1G23850
fragaria_vesca FvH4_3g33230 FvH4_4g34710 FvH4_4g34711 FvH4_4g34720 FvH4_4g34730 FvH4_4g34740 FvH4_4g34760 FvH4_4g34761 FvH4_6g20500 FvH4_6g20520
malus_domestica MD13G1090000.v1.1 MD16G1090300.v1.1 MD16G1090400.v1.1 MD16G1090500.v1.1 MD16G1090600.v1.1
prunus_persica Prupe.1G255400_v2.0.a1 Prupe.1G255600_v2.0.a1 Prupe.1G255700_v2.0.a1 Prupe.1G255800_v2.0.a1
pyrus_communis pycom16g07740 pycom16g07760
rosa_chinensis RchiOBHm_Chr3g0476381 RchiOBHm_Chr4g0444081 RchiOBHm_Chr4g0444091 RchiOBHm_Chr4g0444101
rosa_laevigata RLG00000005856 RLG00000005857 RLG00000005858 RLG00000005859 RLG00000005860 RLG00000023773 RLG00000023775
rosa_multiflora Rmu_co8429785.1_g000001 Rmu_sc0000114.1_g000007 Rmu_sc0002177.1_g000009 Rmu_sc0012638.1_g000002 Rmu_sc0012638.1_g000003
rosa_roxburghii Rroxscaffold_5G00384630 Rroxscaffold_5G00384650 Rroxscaffold_5G00384660 Rroxscaffold_5G00384670 Rroxscaffold_5G00384680 Rroxscaffold_5G00384690 Rroxscaffold_6G00405320 Rroxscaffold_6G00405350
rosa_rugosa Rorug03G0153900 Rorug03G0154000 Rorug04G0349500 Rorug04G0349500 Rorug04G0349500
rosa_samantha Rh3AG205100 Rh3BG236700 Rh3CG231200 Rh3DG231000 Rh4AG410200 Rh4AG410300 Rh4AG410400 Rh4AG410500 Rh4AG410600 Rh4BG421300 Rh4BG421400 Rh4BG421500 Rh4BG421600 Rh4BG421700 Rh4BG421800 Rh4BG421900 Rh4BG422000 Rh4CG435700 Rh4CG435800 Rh4CG435900 Rh4CG436000 Rh4CG436100 Rh4CG436200 Rh4CG436300 Rh4CG436400 Rh4DG416600 Rh4DG416800 Rh4DG416900 Rh4DG417000 Rh4DG417100 Rh4DG417200 Rh4DG417300
rosa_wichuraiana Rw3G018640 Rw4G035240 Rw4G035250 Rw4G035260 Rw4G035270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 902
AccIII TCCGGA 1 cut(s) 552
AciI CCGC 2 cut(s) 900, 902
AcoI YGGCCR 1 cut(s) 903
AcsI RAATTY 2 cut(s) 276, 779
AfaI GTAC 1 cut(s) 321
AflII CTTAAG 1 cut(s) 622
AgsI TTSAA 4 cut(s) 62, 275, 668, 754
AjnI CCWGG 1 cut(s) 579
AluBI AGCT 1 cut(s) 691
AluI AGCT 1 cut(s) 691
Alw21I GWGCWC 1 cut(s) 192
Alw44I GTGCAC 1 cut(s) 188
Ama87I CYCGRG 2 cut(s) 10, 774
Aor13HI TCCGGA 1 cut(s) 552
AoxI GGCC 5 cut(s) 506, 577, 582, 897, 903
ApaLI GTGCAC 1 cut(s) 188
ApeKI GCWGC 3 cut(s) 338, 479, 525
ApoI RAATTY 2 cut(s) 276, 779
AspLEI GCGC 1 cut(s) 611
AsuHPI GGTGA 2 cut(s) 37, 881
AsuII TTCGAA 2 cut(s) 85, 397
AsuNHI GCTAGC 1 cut(s) 356
AvaI CYCGRG 2 cut(s) 10, 774
BaeGI GKGCMC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 259
BauI CACGAG 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 325, 466, 512
BccI CCATC 1 cut(s) 338
BceAI ACGGC 3 cut(s) 493, 592, 890
BciT130I CCWGG 1 cut(s) 581
BfaI CTAG 4 cut(s) 177, 260, 357, 534
BfrI CTTAAG 1 cut(s) 622
BisI GCNGC 5 cut(s) 339, 480, 526, 900, 903
BlsI GCNGC 5 cut(s) 340, 481, 527, 901, 904
Bme1390I CCNGG 1 cut(s) 581
BmeT110I CYCGRG 2 cut(s) 10, 774
BmrFI CCNGG 1 cut(s) 581
BmsI GCATC 1 cut(s) 18
BmtI GCTAGC 1 cut(s) 360
Bpu14I TTCGAA 2 cut(s) 85, 397
BsaJI CCNNGG 2 cut(s) 301, 900
BsaWI WCCGGW 1 cut(s) 552
Bse3DI GCAATG 2 cut(s) 38, 367
BseAI TCCGGA 1 cut(s) 552
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 2 cut(s) 301, 900
BseGI GGATG 1 cut(s) 330
BseMI GCAATG 2 cut(s) 38, 367
BseMII CTCAG 1 cut(s) 12
BseRI GAGGAG 1 cut(s) 662
BseSI GKGCMC 1 cut(s) 192
BseX3I CGGCCG 1 cut(s) 903
BseXI GCAGC 3 cut(s) 325, 466, 512
Bsh1236I CGCG 1 cut(s) 902
Bsh1285I CGRYCG 1 cut(s) 906
BshFI GGCC 5 cut(s) 508, 579, 584, 899, 905
BsiEI CGRYCG 1 cut(s) 906
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 2 cut(s) 10, 774
BsiSI CCGG 2 cut(s) 544, 553
BsnI GGCC 5 cut(s) 508, 579, 584, 899, 905
BsoBI CYCGRG 2 cut(s) 10, 774
Bsp119I TTCGAA 2 cut(s) 85, 397
Bsp1286I GDGCHC 2 cut(s) 192, 259
Bsp13I TCCGGA 1 cut(s) 552
Bsp143I GATC 1 cut(s) 6
BspACI CCGC 2 cut(s) 900, 902
BspANI GGCC 5 cut(s) 508, 579, 584, 899, 905
BspCNI CTCAG 1 cut(s) 13
BspEI TCCGGA 1 cut(s) 552
BspFNI CGCG 1 cut(s) 902
BspOI GCTAGC 1 cut(s) 360
BspQI GCTCTTC 1 cut(s) 554
BspT104I TTCGAA 2 cut(s) 85, 397
BspTI CTTAAG 1 cut(s) 622
BsrDI GCAATG 2 cut(s) 38, 367
BssECI CCNNGG 2 cut(s) 301, 900
BssMI GATC 1 cut(s) 6
BssSI CACGAG 1 cut(s) 425
Bst2BI CACGAG 1 cut(s) 425
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 3 cut(s) 615, 862, 887
Bst6I CTCTTC 1 cut(s) 554
BstAFI CTTAAG 1 cut(s) 622
BstBI TTCGAA 2 cut(s) 85, 397
BstC8I GCNNGC 3 cut(s) 192, 358, 762
BstDEI CTNAG 2 cut(s) 21, 710
BstDSI CCRYGG 1 cut(s) 900
BstF5I GGATG 1 cut(s) 330
BstFNI CGCG 1 cut(s) 902
BstHHI GCGC 1 cut(s) 611
BstKTI GATC 1 cut(s) 9
BstMBI GATC 1 cut(s) 6
BstMCI CGRYCG 1 cut(s) 906
BstMWI GCNNNNNNNGC 2 cut(s) 677, 770
BstNI CCWGG 1 cut(s) 581
BstSCI CCNGG 1 cut(s) 579
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 1 cut(s) 902
BstV1I GCAGC 3 cut(s) 325, 466, 512
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BstZI CGGCCG 1 cut(s) 903
BsuRI GGCC 5 cut(s) 508, 579, 584, 899, 905
BtgI CCRYGG 1 cut(s) 900
BtsCI GGATG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 411
Cac8I GCNNGC 3 cut(s) 192, 358, 762
CfoI GCGC 1 cut(s) 611
Cfr42I CCGCGG 1 cut(s) 903
Csp6I GTAC 1 cut(s) 320
CviAII CATG 3 cut(s) 252, 335, 823
CviQI GTAC 1 cut(s) 320
DdeI CTNAG 2 cut(s) 21, 710
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
EaeI YGGCCR 1 cut(s) 903
EagI CGGCCG 1 cut(s) 903
Eam1104I CTCTTC 1 cut(s) 554
EarI CTCTTC 1 cut(s) 554
EclXI CGGCCG 1 cut(s) 903
Eco24I GRGCYC 1 cut(s) 259
Eco52I CGGCCG 1 cut(s) 903
Eco88I CYCGRG 2 cut(s) 10, 774
EcoRII CCWGG 1 cut(s) 579
EcoT38I GRGCYC 1 cut(s) 259
FaeI CATG 3 cut(s) 255, 338, 826
FatI CATG 3 cut(s) 251, 334, 822
Fnu4HI GCNGC 5 cut(s) 339, 480, 526, 900, 903
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 1 cut(s) 259
Fsp4HI GCNGC 5 cut(s) 339, 480, 526, 900, 903
FspBI CTAG 4 cut(s) 177, 260, 357, 534
GlaI GCGC 1 cut(s) 610
GluI GCNGC 5 cut(s) 339, 480, 526, 900, 903
HaeIII GGCC 5 cut(s) 508, 579, 584, 899, 905
HapII CCGG 2 cut(s) 544, 553
HhaI GCGC 1 cut(s) 611
Hin1II CATG 3 cut(s) 255, 338, 826
Hin6I GCGC 1 cut(s) 609
HinP1I GCGC 1 cut(s) 609
HinfI GANTC 3 cut(s) 37, 62, 211
HpaII CCGG 2 cut(s) 544, 553
HphI GGTGA 2 cut(s) 37, 881
Hpy166II GTNNAC 2 cut(s) 190, 574
Hpy188I TCNGA 6 cut(s) 22, 141, 237, 244, 305, 688
Hpy188III TCNNGA 5 cut(s) 10, 106, 260, 553, 776
Hpy8I GTNNAC 2 cut(s) 190, 574
HpyAV CCTTC 3 cut(s) 134, 467, 646
HpyCH4III ACNGT 3 cut(s) 615, 862, 887
HpyCH4IV ACGT 2 cut(s) 47, 937
HpyCH4V TGCA 5 cut(s) 31, 190, 283, 465, 746
HpyF10VI GCNNNNNNNGC 2 cut(s) 677, 770
HpyF3I CTNAG 2 cut(s) 21, 710
HpySE526I ACGT 2 cut(s) 47, 937
Hsp92II CATG 3 cut(s) 255, 338, 826
HspAI GCGC 1 cut(s) 609
Kpn2I TCCGGA 1 cut(s) 552
KspI CCGCGG 1 cut(s) 903
Kzo9I GATC 1 cut(s) 6
LguI GCTCTTC 1 cut(s) 554
LpnPI CCDG 8 cut(s) 83, 185, 557, 566, 566, 593, 774, 909
Lsp1109I GCAGC 3 cut(s) 325, 466, 512
LweI GCATC 1 cut(s) 18
MaeI CTAG 4 cut(s) 177, 260, 357, 534
MaeII ACGT 2 cut(s) 47, 937
MaeIII GTNAC 2 cut(s) 887, 907
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 9 cut(s) 16, 383, 386, 436, 465, 532, 541, 568, 811
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 2 cut(s) 192, 259
MluCI AATT 5 cut(s) 94, 155, 276, 754, 779
MlyI GAGTC 1 cut(s) 220
MmeI TCCRAC 1 cut(s) 252
MroI TCCGGA 1 cut(s) 552
MseI TTAA 2 cut(s) 623, 940
MspA1I CMGCKG 1 cut(s) 902
MspCI CTTAAG 1 cut(s) 622
MspI CCGG 2 cut(s) 544, 553
MspR9I CCNGG 1 cut(s) 581
MunI CAATTG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 581
MvnI CGCG 1 cut(s) 902
MwoI GCNNNNNNNGC 2 cut(s) 677, 770
NdeII GATC 1 cut(s) 6
NheI GCTAGC 1 cut(s) 356
NlaIII CATG 3 cut(s) 255, 338, 826
NmeAIII GCCGAG 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 887, 907
NspV TTCGAA 2 cut(s) 85, 397
PciSI GCTCTTC 1 cut(s) 554
PfeI GAWTC 2 cut(s) 37, 62
PkrI GCNGC 5 cut(s) 340, 481, 527, 901, 904
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PsuI RGATCY 1 cut(s) 6
RsaI GTAC 1 cut(s) 321
RsaNI GTAC 1 cut(s) 320
SacII CCGCGG 1 cut(s) 903
SapI GCTCTTC 1 cut(s) 554
SaqAI TTAA 2 cut(s) 623, 940
SatI GCNGC 5 cut(s) 339, 480, 526, 900, 903
Sau3AI GATC 1 cut(s) 6
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 1 cut(s) 581
SduI GDGCHC 2 cut(s) 192, 259
SfaNI GCATC 1 cut(s) 18
Sfr303I CCGCGG 1 cut(s) 903
SfuI TTCGAA 2 cut(s) 85, 397
SgrBI CCGCGG 1 cut(s) 903
SmlI CTYRAG 1 cut(s) 622
SmoI CTYRAG 1 cut(s) 622
Sse9I AATT 5 cut(s) 94, 155, 276, 754, 779
SsiI CCGC 2 cut(s) 900, 902
SspMI CTAG 4 cut(s) 177, 260, 357, 534
StyD4I CCNGG 1 cut(s) 579
TaaI ACNGT 3 cut(s) 615, 862, 887
TaiI ACGT 2 cut(s) 50, 940
TaqI TCGA 2 cut(s) 85, 397
TasI AATT 5 cut(s) 94, 155, 276, 754, 779
TatI WGTACW 1 cut(s) 319
TauI GCSGC 2 cut(s) 902, 905
TfiI GAWTC 2 cut(s) 37, 62
Tru1I TTAA 2 cut(s) 623, 940
Tru9I TTAA 2 cut(s) 623, 940
TscAI CASTG 1 cut(s) 411
TseFI GTSAC 2 cut(s) 887, 907
TseI GCWGC 3 cut(s) 338, 479, 525
Tsp45I GTSAC 2 cut(s) 887, 907
TspDTI ATGAA 5 cut(s) 17, 88, 240, 425, 811
TspRI CASTG 1 cut(s) 411
Vha464I CTTAAG 1 cut(s) 622
VneI GTGCAC 1 cut(s) 188
XapI RAATTY 2 cut(s) 276, 779
XbaI TCTAGA 1 cut(s) 259
XspI CTAG 4 cut(s) 177, 260, 357, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.