Rorug03G0153900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000475
Physical Location & Seq
Forward (+)
12655869 .. 12658475
2607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0153900.1

Sequence Viewer

Length: 870 bp
ATGATCAATCCCTCCTCGTCCAATCCCCAACCCATGTCCTCCGACGCCTCCGGTAAGAAGGTCCGGAAGCCTTACACCATCACCAAGTCCAGAGAGAGCTGGACCGAGGAAGAGCACGACAAGTTCCTTGAAGCTCTTCAACTGTTTGATCGTGACTGGAAGAAAATTGAAGATTTTGTGGGTTCTAAAACGGTTATTCAGATTCGAAGTCATGCGCAGAAGTACTTCTTGAAAGTTCAAAAGAATGGGACAACAGCACATGTGCCTCCTCCTCGGCCAAAGCGCAAGGCTTCTCATCCTTACCCGCAAAAAGCCTCCAAAAATGTTTTAATTCCCTTGCAAGCATCACTTGCTTATCCTTCTTCCATGAATGCCATTGCACCTGGCTATTGTCCTTGGGATGAAGCTTCGATTTTCTTAAATGCTGGATCAGACCAAATTGTGTTGTCACACGAAGAATTTATTAATCGTCAAGGAACGGAAGCTGATATTGGATCAAAGGGGGTAGCAAGAATTAGTACATGCGATCCTAGTGGTATCGCAAGCTCAAGTAGAACCCTACCTAGTTCCGAGATACCAAAGCAAGGGTCACAAGCTCCTGTCAGCCATGGTATTCCTGATTTTGCCGAAGTTTATAGCTTCATTGGAGGTGTGTTTGATCCGGACACCCAAGGCCATGCTCAGAAACTCAAGGAAATGGATCCCATTAATTTTGAAACTGTTTTGTTGTTGATGAGAAACCTCAGCATCAACTTGTCTAGCCCAGATTTTGAACCAATTAGGAAGGTCCTGGCTTCAAATGATGCCAATATGAAAACAGTAGCTGCAGGAAATATTCCCAGTTACCGGAATAACGATCTTTCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

289

Amino Acids

31.86

Weight (kDa)

8.39

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 31 - 76 1.2e-12 Myb-like DNA-binding domain
RVE6 PF24904 205 - 266 1.3e-33 REVEILLE 6-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000475)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23830 AT1G23840 AT1G23850
fragaria_vesca FvH4_3g33230 FvH4_4g34710 FvH4_4g34711 FvH4_4g34720 FvH4_4g34730 FvH4_4g34740 FvH4_4g34760 FvH4_4g34761 FvH4_6g20500 FvH4_6g20520
malus_domestica MD13G1090000.v1.1 MD16G1090300.v1.1 MD16G1090400.v1.1 MD16G1090500.v1.1 MD16G1090600.v1.1
prunus_persica Prupe.1G255400_v2.0.a1 Prupe.1G255600_v2.0.a1 Prupe.1G255700_v2.0.a1 Prupe.1G255800_v2.0.a1
pyrus_communis pycom16g07740 pycom16g07760
rosa_chinensis RchiOBHm_Chr3g0476381 RchiOBHm_Chr4g0444081 RchiOBHm_Chr4g0444091 RchiOBHm_Chr4g0444101
rosa_laevigata RLG00000005856 RLG00000005857 RLG00000005858 RLG00000005859 RLG00000005860 RLG00000023773 RLG00000023775
rosa_multiflora Rmu_co8429785.1_g000001 Rmu_sc0000114.1_g000007 Rmu_sc0002177.1_g000009 Rmu_sc0012638.1_g000002 Rmu_sc0012638.1_g000003
rosa_roxburghii Rroxscaffold_5G00384630 Rroxscaffold_5G00384650 Rroxscaffold_5G00384660 Rroxscaffold_5G00384670 Rroxscaffold_5G00384680 Rroxscaffold_5G00384690 Rroxscaffold_6G00405320 Rroxscaffold_6G00405350
rosa_rugosa Rorug03G0153900 Rorug03G0154000 Rorug04G0349500 Rorug04G0349500 Rorug04G0349500
rosa_samantha Rh3AG205100 Rh3BG236700 Rh3CG231200 Rh3DG231000 Rh4AG410200 Rh4AG410300 Rh4AG410400 Rh4AG410500 Rh4AG410600 Rh4BG421300 Rh4BG421400 Rh4BG421500 Rh4BG421600 Rh4BG421700 Rh4BG421800 Rh4BG421900 Rh4BG422000 Rh4CG435700 Rh4CG435800 Rh4CG435900 Rh4CG436000 Rh4CG436100 Rh4CG436200 Rh4CG436300 Rh4CG436400 Rh4DG416600 Rh4DG416800 Rh4DG416900 Rh4DG417000 Rh4DG417100 Rh4DG417200 Rh4DG417300
rosa_wichuraiana Rw3G018640 Rw4G035240 Rw4G035250 Rw4G035260 Rw4G035270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 216
AccIII TCCGGA 2 cut(s) 63, 661
AciI CCGC 1 cut(s) 305
AclWI GGATC 6 cut(s) 436, 502, 521, 653, 695, 708
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 1 cut(s) 458
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 224, 520
AfiI CCNNNNNNNGG 2 cut(s) 584, 846
AflIII ACRYGT 1 cut(s) 259
AgsI TTSAA 8 cut(s) 131, 140, 170, 232, 239, 716, 773, 798
AjnI CCWGG 2 cut(s) 382, 789
AjuI GAANNNNNNNTTGG 2 cut(s) 474, 506
AluBI AGCT 8 cut(s) 99, 134, 407, 485, 546, 596, 639, 824
AluI AGCT 8 cut(s) 99, 134, 407, 485, 546, 596, 639, 824
Alw21I GWGCWC 1 cut(s) 117
AlwI GGATC 6 cut(s) 436, 502, 521, 653, 695, 708
AlwNI CAGNNNCTG 1 cut(s) 824
Aor13HI TCCGGA 2 cut(s) 63, 661
AoxI GGCC 2 cut(s) 275, 673
ApeKI GCWGC 1 cut(s) 824
ApoI RAATTY 1 cut(s) 458
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
AseI ATTAAT 2 cut(s) 465, 708
Asp700I GAANNNNTTC 2 cut(s) 135, 224
AspLEI GCGC 2 cut(s) 217, 285
AspS9I GGNCC 3 cut(s) 61, 102, 787
AsuHPI GGTGA 1 cut(s) 73
AsuII TTCGAA 1 cut(s) 205
AvaII GGWCC 3 cut(s) 61, 102, 787
BamHI GGATCC 1 cut(s) 700
Bbv12I GWGCWC 1 cut(s) 117
BbvCI CCTCAGC 1 cut(s) 743
BbvI GCAGC 1 cut(s) 811
BccI CCATC 1 cut(s) 86
BciT130I CCWGG 2 cut(s) 384, 791
BclI TGATCA 1 cut(s) 3
BfaI CTAG 3 cut(s) 531, 564, 759
BfmI CTRYAG 1 cut(s) 825
BisI GCNGC 1 cut(s) 825
BlsI GCNGC 1 cut(s) 826
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 2 cut(s) 384, 791
Bme18I GGWCC 3 cut(s) 61, 102, 787
BmgT120I GGNCC 3 cut(s) 61, 102, 787
BmiI GGNNCC 1 cut(s) 702
BmrFI CCNGG 2 cut(s) 384, 791
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 3 cut(s) 353, 756, 793
BmuI ACTGGG 1 cut(s) 834
Bpu10I CCTNAGC 1 cut(s) 743
Bpu14I TTCGAA 1 cut(s) 205
BpuEI CTTGAG 2 cut(s) 532, 674
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 5 cut(s) 105, 272, 395, 607, 670
BsaWI WCCGGW 4 cut(s) 50, 63, 661, 846
Bsc4I CCNNNNNNNGG 2 cut(s) 584, 846
Bse1I ACTGG 2 cut(s) 161, 840
Bse3DI GCAATG 1 cut(s) 375
BseAI TCCGGA 2 cut(s) 63, 661
BseBI CCWGG 2 cut(s) 384, 791
BseDI CCNNGG 5 cut(s) 105, 272, 395, 607, 670
BseGI GGATG 2 cut(s) 295, 406
BseLI CCNNNNNNNGG 2 cut(s) 584, 846
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 2 cut(s) 695, 757
BseNI ACTGG 2 cut(s) 161, 840
BseRI GAGGAG 3 cut(s) 4, 258, 261
BseXI GCAGC 1 cut(s) 811
BshFI GGCC 2 cut(s) 277, 675
BsiHKAI GWGCWC 1 cut(s) 117
BsiSI CCGG 4 cut(s) 51, 64, 662, 847
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 2 cut(s) 584, 846
BsmFI GGGAC 1 cut(s) 262
BsmI GAATGC 1 cut(s) 376
BsnI GGCC 2 cut(s) 277, 675
Bsp119I TTCGAA 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 117
Bsp13I TCCGGA 2 cut(s) 63, 661
Bsp143I GATC 8 cut(s) 3, 148, 428, 494, 526, 658, 700, 856
Bsp19I CCATGG 1 cut(s) 607
BspACI CCGC 1 cut(s) 305
BspANI GGCC 2 cut(s) 277, 675
BspCNI CTCAG 2 cut(s) 694, 756
BspEI TCCGGA 2 cut(s) 63, 661
BspLI GGNNCC 1 cut(s) 702
BspMAI CTGCAG 1 cut(s) 829
BspPI GGATC 6 cut(s) 436, 502, 521, 653, 695, 708
BspQI GCTCTTC 2 cut(s) 105, 141
BspT104I TTCGAA 1 cut(s) 205
BsrDI GCAATG 1 cut(s) 375
BsrI ACTGG 2 cut(s) 161, 840
BssECI CCNNGG 5 cut(s) 105, 272, 395, 607, 670
BssMI GATC 8 cut(s) 3, 148, 428, 494, 526, 658, 700, 856
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 3 cut(s) 395, 607, 670
Bst2UI CCWGG 2 cut(s) 384, 791
Bst4CI ACNGT 4 cut(s) 144, 193, 721, 820
Bst6I CTCTTC 2 cut(s) 105, 141
BstACI GRCGYC 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 350
BstBI TTCGAA 1 cut(s) 205
BstC8I GCNNGC 2 cut(s) 342, 544
BstDEI CTNAG 2 cut(s) 681, 743
BstDSI CCRYGG 1 cut(s) 607
BstF5I GGATG 2 cut(s) 295, 406
BstHHI GCGC 2 cut(s) 217, 285
BstKTI GATC 8 cut(s) 6, 151, 431, 497, 529, 661, 703, 859
BstMBI GATC 8 cut(s) 3, 148, 428, 494, 526, 658, 700, 856
BstMWI GCNNNNNNNGC 1 cut(s) 350
BstNI CCWGG 2 cut(s) 384, 791
BstNSI RCATGY 2 cut(s) 263, 525
BstSCI CCNGG 2 cut(s) 382, 789
BstSFI CTRYAG 1 cut(s) 825
BstV1I GCAGC 1 cut(s) 811
BstX2I RGATCY 1 cut(s) 700
BstYI RGATCY 1 cut(s) 700
BsuRI GGCC 2 cut(s) 277, 675
BtgI CCRYGG 1 cut(s) 607
BtsCI GGATG 2 cut(s) 295, 406
Cac8I GCNNGC 2 cut(s) 342, 544
CaiI CAGNNNCTG 1 cut(s) 824
CfoI GCGC 2 cut(s) 217, 285
Cfr13I GGNCC 3 cut(s) 61, 102, 787
CseI GACGC 1 cut(s) 53
Csp6I GTAC 2 cut(s) 223, 519
CviAII CATG 7 cut(s) 34, 212, 260, 367, 522, 608, 677
CviQI GTAC 2 cut(s) 223, 519
DdeI CTNAG 2 cut(s) 681, 743
DpnI GATC 8 cut(s) 5, 150, 430, 496, 528, 660, 702, 858
DpnII GATC 8 cut(s) 3, 148, 428, 494, 526, 658, 700, 856
EaeI YGGCCR 1 cut(s) 275
Eam1104I CTCTTC 2 cut(s) 105, 141
EarI CTCTTC 2 cut(s) 105, 141
Eco130I CCWWGG 3 cut(s) 395, 607, 670
Eco47I GGWCC 3 cut(s) 61, 102, 787
EcoO109I RGGNCCY 1 cut(s) 787
EcoRII CCWGG 2 cut(s) 382, 789
EcoT14I CCWWGG 3 cut(s) 395, 607, 670
ErhI CCWWGG 3 cut(s) 395, 607, 670
FaeI CATG 7 cut(s) 37, 215, 263, 370, 525, 611, 680
FaiI YATR 9 cut(s) 35, 213, 261, 368, 523, 609, 636, 678, 812
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 333, 365
FaqI GGGAC 1 cut(s) 262
FatI CATG 7 cut(s) 33, 211, 259, 366, 521, 607, 676
FauI CCCGC 1 cut(s) 312
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 825
FokI GGATG 2 cut(s) 282, 413
Fsp4HI GCNGC 1 cut(s) 825
FspBI CTAG 3 cut(s) 531, 564, 759
FspI TGCGCA 1 cut(s) 216
GlaI GCGC 2 cut(s) 216, 284
GluI GCNGC 1 cut(s) 825
HaeIII GGCC 2 cut(s) 277, 675
HapII CCGG 4 cut(s) 51, 64, 662, 847
HgaI GACGC 1 cut(s) 53
HhaI GCGC 2 cut(s) 217, 285
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 7 cut(s) 37, 215, 263, 370, 525, 611, 680
Hin6I GCGC 2 cut(s) 215, 283
HinP1I GCGC 2 cut(s) 215, 283
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 1 cut(s) 202
HpaII CCGG 4 cut(s) 51, 64, 662, 847
HphI GGTGA 1 cut(s) 73
Hpy188I TCNGA 5 cut(s) 43, 201, 433, 571, 684
Hpy188III TCNNGA 6 cut(s) 64, 90, 152, 229, 617, 662
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 3 cut(s) 52, 369, 778
HpyCH4III ACNGT 4 cut(s) 144, 193, 721, 820
HpyCH4V TGCA 3 cut(s) 340, 380, 827
HpyF10VI GCNNNNNNNGC 1 cut(s) 350
HpyF3I CTNAG 2 cut(s) 681, 743
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 7 cut(s) 37, 215, 263, 370, 525, 611, 680
HspAI GCGC 2 cut(s) 215, 283
Kpn2I TCCGGA 2 cut(s) 63, 661
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 8 cut(s) 3, 148, 428, 494, 526, 658, 700, 856
LguI GCTCTTC 2 cut(s) 105, 141
LmnI GCTCC 1 cut(s) 601
Lsp1109I GCAGC 1 cut(s) 811
LweI GCATC 3 cut(s) 353, 756, 793
MaeI CTAG 3 cut(s) 531, 564, 759
MaeIII GTNAC 4 cut(s) 152, 447, 588, 842
MalI GATC 8 cut(s) 5, 150, 430, 496, 528, 660, 702, 858
MboI GATC 8 cut(s) 3, 148, 428, 494, 526, 658, 700, 856
MboII GAAGA 6 cut(s) 122, 128, 172, 182, 354, 467
MflI RGATCY 1 cut(s) 700
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 7 cut(s) 165, 330, 438, 458, 513, 709, 777
MmeI TCCRAC 1 cut(s) 66
MroI TCCGGA 2 cut(s) 63, 661
MroXI GAANNNNTTC 2 cut(s) 135, 224
MseI TTAA 4 cut(s) 329, 419, 465, 708
MspI CCGG 4 cut(s) 51, 64, 662, 847
MspR9I CCNGG 2 cut(s) 384, 791
Mva1269I GAATGC 1 cut(s) 376
MvaI CCWGG 2 cut(s) 384, 791
MwoI GCNNNNNNNGC 1 cut(s) 350
NcoI CCATGG 1 cut(s) 607
NdeII GATC 8 cut(s) 3, 148, 428, 494, 526, 658, 700, 856
NlaIII CATG 7 cut(s) 37, 215, 263, 370, 525, 611, 680
NlaIV GGNNCC 1 cut(s) 702
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 3 cut(s) 152, 447, 588
NsbI TGCGCA 1 cut(s) 216
NspI RCATGY 2 cut(s) 263, 525
NspV TTCGAA 1 cut(s) 205
PciI ACATGT 1 cut(s) 259
PciSI GCTCTTC 2 cut(s) 105, 141
PctI GAATGC 1 cut(s) 376
PdmI GAANNNNTTC 2 cut(s) 135, 224
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 1 cut(s) 826
PpuMI RGGWCCY 1 cut(s) 787
PscI ACATGT 1 cut(s) 259
PshBI ATTAAT 2 cut(s) 465, 708
Psp5II RGGWCCY 1 cut(s) 787
Psp6I CCWGG 2 cut(s) 382, 789
PspGI CCWGG 2 cut(s) 382, 789
PspN4I GGNNCC 1 cut(s) 702
PspPI GGNCC 3 cut(s) 61, 102, 787
PspPPI RGGWCCY 1 cut(s) 787
PstI CTGCAG 1 cut(s) 829
PstNI CAGNNNCTG 1 cut(s) 824
PsuI RGATCY 1 cut(s) 700
RsaI GTAC 2 cut(s) 224, 520
RsaNI GTAC 2 cut(s) 223, 519
SapI GCTCTTC 2 cut(s) 105, 141
SaqAI TTAA 4 cut(s) 329, 419, 465, 708
SatI GCNGC 1 cut(s) 825
Sau3AI GATC 8 cut(s) 3, 148, 428, 494, 526, 658, 700, 856
Sau96I GGNCC 3 cut(s) 61, 102, 787
ScaI AGTACT 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 384, 791
SduI GDGCHC 1 cut(s) 117
SfaNI GCATC 3 cut(s) 353, 756, 793
SfcI CTRYAG 1 cut(s) 825
SfuI TTCGAA 1 cut(s) 205
SinI GGWCC 3 cut(s) 61, 102, 787
SmlI CTYRAG 2 cut(s) 547, 689
SmoI CTYRAG 2 cut(s) 547, 689
Sse9I AATT 7 cut(s) 165, 330, 438, 458, 513, 709, 777
SsiI CCGC 1 cut(s) 305
SspI AATATT 1 cut(s) 835
SspMI CTAG 3 cut(s) 531, 564, 759
StyD4I CCNGG 2 cut(s) 382, 789
StyI CCWWGG 3 cut(s) 395, 607, 670
TaaI ACNGT 4 cut(s) 144, 193, 721, 820
TaqI TCGA 2 cut(s) 205, 410
TaqII GACCGA 1 cut(s) 119
TasI AATT 7 cut(s) 165, 330, 438, 458, 513, 709, 777
TatI WGTACW 2 cut(s) 222, 518
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 4 cut(s) 329, 419, 465, 708
Tru9I TTAA 4 cut(s) 329, 419, 465, 708
TseFI GTSAC 3 cut(s) 152, 447, 588
TseI GCWGC 1 cut(s) 824
Tsp45I GTSAC 3 cut(s) 152, 447, 588
TspDTI ATGAA 5 cut(s) 383, 417, 631, 827, 852
TspGWI ACGGA 1 cut(s) 494
VpaK11BI GGWCC 3 cut(s) 61, 102, 787
VspI ATTAAT 2 cut(s) 465, 708
XapI RAATTY 1 cut(s) 458
XceI RCATGY 2 cut(s) 263, 525
XmnI GAANNNNTTC 2 cut(s) 135, 224
XspI CTAG 3 cut(s) 531, 564, 759
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.