Rh4CG436400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000475
Physical Location & Seq
Reverse (-)
67438970 .. 67439588
619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG436400.1

Sequence Viewer

Length: 561 bp
ATGGAGAAGAAGTCTAGGTTTCTAGGCATCTTTGGTATCATCAGAGTAGCCATAGAAATCCCTTTCATCAATCCCAGTTTCATTGCCTTCACATTTATCACATCCCTTCCTCTATTTTGCATAATGGTGTTACCAAAATTGCCATTCGAGCCTGCTAGAATCAGGGAGGCATTAGTTAAGCTAGAACAGCTTAATTCCCACTCCAACAAACTCATATCTGTTACCTCATGGCTTCTAAATGACACCATTAGAGTTGGCACGGTTCAACTTTGCAAACTAATCACCTTCCTGGCCACGGTCACTCTTATTGATTATGCTTCAACAATATATAAGGGCGGAGCTGGGAGGTCAGTGGGTCTCAGAGATCTTTTACGAAACAATTTCAGAAATAGGGGGGATAGCTCTATGTTGGCATACTTAGTCATGTCCTGGTTCTCAAAGATGTCTTTGGACGCTGTTCTCCTATATGGTACTGCAGGTCCGTTGGGTTCATTAAGAAATGAGTGGGCGCCGCCTCTACATTTCTTCACTCAGGTTGGGGTCTCTTTCGTGCACCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.87

Weight (kDa)

9.88

Isoelectric Point (pI)

34.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000475)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23830 AT1G23840 AT1G23850
fragaria_vesca FvH4_3g33230 FvH4_4g34710 FvH4_4g34711 FvH4_4g34720 FvH4_4g34730 FvH4_4g34740 FvH4_4g34760 FvH4_4g34761 FvH4_6g20500 FvH4_6g20520
malus_domestica MD13G1090000.v1.1 MD16G1090300.v1.1 MD16G1090400.v1.1 MD16G1090500.v1.1 MD16G1090600.v1.1
prunus_persica Prupe.1G255400_v2.0.a1 Prupe.1G255600_v2.0.a1 Prupe.1G255700_v2.0.a1 Prupe.1G255800_v2.0.a1
pyrus_communis pycom16g07740 pycom16g07760
rosa_chinensis RchiOBHm_Chr3g0476381 RchiOBHm_Chr4g0444081 RchiOBHm_Chr4g0444091 RchiOBHm_Chr4g0444101
rosa_laevigata RLG00000005856 RLG00000005857 RLG00000005858 RLG00000005859 RLG00000005860 RLG00000023773 RLG00000023775
rosa_multiflora Rmu_co8429785.1_g000001 Rmu_sc0000114.1_g000007 Rmu_sc0002177.1_g000009 Rmu_sc0012638.1_g000002 Rmu_sc0012638.1_g000003
rosa_roxburghii Rroxscaffold_5G00384630 Rroxscaffold_5G00384650 Rroxscaffold_5G00384660 Rroxscaffold_5G00384670 Rroxscaffold_5G00384680 Rroxscaffold_5G00384690 Rroxscaffold_6G00405320 Rroxscaffold_6G00405350
rosa_rugosa Rorug03G0153900 Rorug03G0154000 Rorug04G0349500 Rorug04G0349500 Rorug04G0349500
rosa_samantha Rh3AG205100 Rh3BG236700 Rh3CG231200 Rh3DG231000 Rh4AG410200 Rh4AG410300 Rh4AG410400 Rh4AG410500 Rh4AG410600 Rh4BG421300 Rh4BG421400 Rh4BG421500 Rh4BG421600 Rh4BG421700 Rh4BG421800 Rh4BG421900 Rh4BG422000 Rh4CG435700 Rh4CG435800 Rh4CG435900 Rh4CG436000 Rh4CG436100 Rh4CG436200 Rh4CG436300 Rh4CG436400 Rh4DG416600 Rh4DG416800 Rh4DG416900 Rh4DG417000 Rh4DG417100 Rh4DG417200 Rh4DG417300
rosa_wichuraiana Rw3G018640 Rw4G035240 Rw4G035250 Rw4G035260 Rw4G035270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 508
AciI CCGC 2 cut(s) 336, 512
AcoI YGGCCR 1 cut(s) 291
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 1 cut(s) 295
AgsI TTSAA 2 cut(s) 266, 321
AjnI CCWGG 2 cut(s) 288, 428
AluBI AGCT 4 cut(s) 181, 190, 341, 402
AluI AGCT 4 cut(s) 181, 190, 341, 402
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 2 cut(s) 362, 547
Alw44I GTGCAC 1 cut(s) 551
AoxI GGCC 1 cut(s) 291
ApaLI GTGCAC 1 cut(s) 551
AspLEI GCGC 1 cut(s) 511
AspS9I GGNCC 1 cut(s) 479
AsuHPI GGTGA 1 cut(s) 274
AvaII GGWCC 1 cut(s) 479
BaeGI GKGCMC 1 cut(s) 555
BalI TGGCCA 1 cut(s) 293
BanI GGYRCC 1 cut(s) 508
Bbv12I GWGCWC 1 cut(s) 555
BciT130I CCWGG 2 cut(s) 290, 430
BcoDI GTCTC 2 cut(s) 362, 547
BfaI CTAG 5 cut(s) 15, 23, 156, 182, 559
BfmI CTRYAG 1 cut(s) 474
BfoI RGCGCY 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 467
BglII AGATCT 1 cut(s) 364
BisI GCNGC 1 cut(s) 512
BlsI GCNGC 1 cut(s) 513
Bme1390I CCNGG 2 cut(s) 290, 430
Bme18I GGWCC 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 479
BmiI GGNNCC 1 cut(s) 510
BmrFI CCNGG 2 cut(s) 290, 430
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 1 cut(s) 36
BmuI ACTGGG 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 509
BsaI GGTCTC 2 cut(s) 362, 547
BsaJI CCNNGG 1 cut(s) 294
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse1I ACTGG 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 81
BseBI CCWGG 2 cut(s) 290, 430
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 1 cut(s) 101
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 2 cut(s) 373, 545
BseNI ACTGG 1 cut(s) 75
BseSI GKGCMC 1 cut(s) 555
BseYI CCCAGC 1 cut(s) 341
BshFI GGCC 1 cut(s) 293
BshNI GGYRCC 1 cut(s) 508
BsiHKAI GWGCWC 1 cut(s) 555
BslI CCNNNNNNNGG 1 cut(s) 295
BsmAI GTCTC 2 cut(s) 362, 547
BsnI GGCC 1 cut(s) 293
Bso31I GGTCTC 2 cut(s) 362, 547
Bsp1286I GDGCHC 1 cut(s) 555
Bsp143I GATC 1 cut(s) 364
BspACI CCGC 2 cut(s) 336, 512
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 2 cut(s) 372, 544
BspLI GGNNCC 1 cut(s) 510
BspMAI CTGCAG 1 cut(s) 478
BspMI ACCTGC 1 cut(s) 467
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 2 cut(s) 362, 547
BsrDI GCAATG 1 cut(s) 81
BsrI ACTGG 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 1 cut(s) 364
BssNI GRCGYC 1 cut(s) 509
Bst2UI CCWGG 2 cut(s) 290, 430
Bst4CI ACNGT 2 cut(s) 262, 298
BstACI GRCGYC 1 cut(s) 509
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 3 cut(s) 359, 418, 531
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 101
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 1 cut(s) 511
BstKTI GATC 1 cut(s) 367
BstMAI GTCTC 2 cut(s) 362, 547
BstMBI GATC 1 cut(s) 364
BstMWI GCNNNNNNNGC 2 cut(s) 148, 187
BstNI CCWGG 2 cut(s) 290, 430
BstSCI CCNGG 2 cut(s) 288, 428
BstSFI CTRYAG 1 cut(s) 474
BstSLI GKGCMC 1 cut(s) 555
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuRI GGCC 1 cut(s) 293
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 357
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 1 cut(s) 153
CfoI GCGC 1 cut(s) 511
Cfr13I GGNCC 1 cut(s) 479
CseI GACGC 1 cut(s) 461
Csp6I GTAC 1 cut(s) 471
CviAII CATG 2 cut(s) 228, 424
CviJI RGCY 8 cut(s) 50, 151, 181, 190, 232, 293, 341, 402
CviKI_1 RGCY 8 cut(s) 50, 151, 181, 190, 232, 293, 341, 402
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 3 cut(s) 359, 418, 531
DinI GGCGCC 1 cut(s) 510
DpnI GATC 1 cut(s) 366
DpnII GATC 1 cut(s) 364
EaeI YGGCCR 1 cut(s) 291
EciI GGCGGA 1 cut(s) 351
Eco31I GGTCTC 2 cut(s) 362, 547
Eco47I GGWCC 1 cut(s) 479
EcoRII CCWGG 2 cut(s) 288, 428
EgeI GGCGCC 1 cut(s) 510
EheI GGCGCC 1 cut(s) 510
FaeI CATG 2 cut(s) 231, 427
FatI CATG 2 cut(s) 227, 423
Fnu4HI GCNGC 1 cut(s) 512
FokI GGATG 1 cut(s) 88
Fsp4HI GCNGC 1 cut(s) 512
FspBI CTAG 5 cut(s) 15, 23, 156, 182, 559
GlaI GCGC 1 cut(s) 510
GluI GCNGC 1 cut(s) 512
GsaI CCCAGC 1 cut(s) 345
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 1 cut(s) 293
HgaI GACGC 1 cut(s) 461
HhaI GCGC 1 cut(s) 511
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 2 cut(s) 231, 427
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HinfI GANTC 1 cut(s) 159
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 3 cut(s) 44, 362, 386
Hpy8I GTNNAC 1 cut(s) 553
HpyAV CCTTC 3 cut(s) 97, 116, 295
HpyCH4III ACNGT 2 cut(s) 262, 298
HpyCH4V TGCA 4 cut(s) 120, 273, 476, 553
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 187
HpyF3I CTNAG 3 cut(s) 359, 418, 531
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 2 cut(s) 231, 427
HspAI GCGC 1 cut(s) 509
KasI GGCGCC 1 cut(s) 508
Kzo9I GATC 1 cut(s) 364
LmnI GCTCC 1 cut(s) 338
LweI GCATC 1 cut(s) 36
MaeI CTAG 5 cut(s) 15, 23, 156, 182, 559
MaeIII GTNAC 3 cut(s) 129, 220, 298
MalI GATC 1 cut(s) 366
MboI GATC 1 cut(s) 364
MboII GAAGA 2 cut(s) 19, 517
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 1 cut(s) 555
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 3 cut(s) 137, 193, 379
MluNI TGGCCA 1 cut(s) 293
Mly113I GGCGCC 1 cut(s) 509
MmeI TCCRAC 1 cut(s) 228
MnlI CCTC 5 cut(s) 120, 160, 235, 339, 525
Mox20I TGGCCA 1 cut(s) 293
MscI TGGCCA 1 cut(s) 293
MseI TTAA 3 cut(s) 177, 192, 494
MslI CAYNNNNRTG 1 cut(s) 125
Msp20I TGGCCA 1 cut(s) 293
MspR9I CCNGG 2 cut(s) 290, 430
MvaI CCWGG 2 cut(s) 290, 430
MwoI GCNNNNNNNGC 2 cut(s) 148, 187
NarI GGCGCC 1 cut(s) 509
NdeII GATC 1 cut(s) 364
NlaIII CATG 2 cut(s) 231, 427
NlaIV GGNNCC 1 cut(s) 510
NmuCI GTSAC 1 cut(s) 298
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 1 cut(s) 513
PluTI GGCGCC 1 cut(s) 512
Psp6I CCWGG 2 cut(s) 288, 428
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 288, 428
PspN4I GGNNCC 1 cut(s) 510
PspPI GGNCC 1 cut(s) 479
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 1 cut(s) 364
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 1 cut(s) 125
SaqAI TTAA 3 cut(s) 177, 192, 494
SatI GCNGC 1 cut(s) 512
Sau3AI GATC 1 cut(s) 364
Sau96I GGNCC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 290, 430
SduI GDGCHC 1 cut(s) 555
SfaNI GCATC 1 cut(s) 36
SfcI CTRYAG 1 cut(s) 474
SfoI GGCGCC 1 cut(s) 510
SinI GGWCC 1 cut(s) 479
SmiMI CAYNNNNRTG 1 cut(s) 125
Sse9I AATT 3 cut(s) 137, 193, 379
SsiI CCGC 2 cut(s) 336, 512
SspDI GGCGCC 1 cut(s) 508
SspMI CTAG 5 cut(s) 15, 23, 156, 182, 559
StyD4I CCNGG 2 cut(s) 288, 428
TaaI ACNGT 2 cut(s) 262, 298
TaqI TCGA 1 cut(s) 147
TasI AATT 3 cut(s) 137, 193, 379
TauI GCSGC 1 cut(s) 514
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 3 cut(s) 177, 192, 494
Tru9I TTAA 3 cut(s) 177, 192, 494
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 1 cut(s) 298
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 3 cut(s) 55, 70, 480
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 1 cut(s) 357
VneI GTGCAC 1 cut(s) 551
VpaK11BI GGWCC 1 cut(s) 479
XspI CTAG 5 cut(s) 15, 23, 156, 182, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.