No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 1 cut(s) | 333 |
| AcsI | RAATTY | 4 cut(s) | 125, 237, 244, 360 |
| AcuI | CTGAAG | 1 cut(s) | 128 |
| AfaI | GTAC | 4 cut(s) | 152, 188, 273, 281 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 219 |
| AgsI | TTSAA | 4 cut(s) | 131, 202, 242, 249 |
| AluBI | AGCT | 3 cut(s) | 53, 71, 179 |
| AluI | AGCT | 3 cut(s) | 53, 71, 179 |
| Alw26I | GTCTC | 1 cut(s) | 200 |
| AoxI | GGCC | 1 cut(s) | 333 |
| ApoI | RAATTY | 4 cut(s) | 125, 237, 244, 360 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 237 |
| BbsI | GAAGAC | 1 cut(s) | 326 |
| BceAI | ACGGC | 1 cut(s) | 348 |
| BclI | TGATCA | 1 cut(s) | 76 |
| BcoDI | GTCTC | 1 cut(s) | 200 |
| BfaI | CTAG | 4 cut(s) | 6, 44, 56, 230 |
| BglII | AGATCT | 2 cut(s) | 139, 298 |
| BmcAI | AGTACT | 1 cut(s) | 188 |
| BmsI | GCATC | 1 cut(s) | 39 |
| BpiI | GAAGAC | 1 cut(s) | 326 |
| BpmI | CTGGAG | 1 cut(s) | 192 |
| BsaJI | CCNNGG | 1 cut(s) | 118 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 219 |
| Bse1I | ACTGG | 2 cut(s) | 175, 230 |
| BseDI | CCNNGG | 1 cut(s) | 118 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 219 |
| BseMII | CTCAG | 1 cut(s) | 302 |
| BseNI | ACTGG | 2 cut(s) | 175, 230 |
| BshFI | GGCC | 1 cut(s) | 335 |
| BslFI | GGGAC | 1 cut(s) | 236 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 219 |
| BsmAI | GTCTC | 1 cut(s) | 200 |
| BsmFI | GGGAC | 1 cut(s) | 236 |
| BsnI | GGCC | 1 cut(s) | 335 |
| Bsp1407I | TGTACA | 1 cut(s) | 279 |
| Bsp143I | GATC | 3 cut(s) | 76, 139, 298 |
| BspANI | GGCC | 1 cut(s) | 335 |
| BspCNI | CTCAG | 1 cut(s) | 303 |
| BsrGI | TGTACA | 1 cut(s) | 279 |
| BsrI | ACTGG | 2 cut(s) | 175, 230 |
| BssECI | CCNNGG | 1 cut(s) | 118 |
| BssMI | GATC | 3 cut(s) | 76, 139, 298 |
| BssT1I | CCWWGG | 1 cut(s) | 118 |
| BstAUI | TGTACA | 1 cut(s) | 279 |
| BstDEI | CTNAG | 1 cut(s) | 311 |
| BstKTI | GATC | 3 cut(s) | 79, 142, 301 |
| BstMAI | GTCTC | 1 cut(s) | 200 |
| BstMBI | GATC | 3 cut(s) | 76, 139, 298 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 10 |
| BstNSI | RCATGY | 1 cut(s) | 373 |
| BstV2I | GAAGAC | 1 cut(s) | 326 |
| BstX2I | RGATCY | 2 cut(s) | 139, 298 |
| BstYI | RGATCY | 2 cut(s) | 139, 298 |
| BsuRI | GGCC | 1 cut(s) | 335 |
| BtsIMutI | CAGTG | 1 cut(s) | 168 |
| Csp6I | GTAC | 4 cut(s) | 151, 187, 272, 280 |
| CviAII | CATG | 2 cut(s) | 370, 374 |
| CviJI | RGCY | 7 cut(s) | 37, 53, 71, 93, 179, 229, 335 |
| CviKI_1 | RGCY | 7 cut(s) | 37, 53, 71, 93, 179, 229, 335 |
| CviQI | GTAC | 4 cut(s) | 151, 187, 272, 280 |
| DdeI | CTNAG | 1 cut(s) | 311 |
| DpnI | GATC | 3 cut(s) | 78, 141, 300 |
| DpnII | GATC | 3 cut(s) | 76, 139, 298 |
| EaeI | YGGCCR | 1 cut(s) | 333 |
| Eco130I | CCWWGG | 1 cut(s) | 118 |
| Eco57I | CTGAAG | 1 cut(s) | 128 |
| EcoT14I | CCWWGG | 1 cut(s) | 118 |
| EcoT22I | ATGCAT | 1 cut(s) | 375 |
| ErhI | CCWWGG | 1 cut(s) | 118 |
| FaeI | CATG | 2 cut(s) | 373, 377 |
| FaiI | YATR | 7 cut(s) | 20, 27, 156, 261, 351, 371, 375 |
| FaqI | GGGAC | 1 cut(s) | 236 |
| FatI | CATG | 2 cut(s) | 369, 373 |
| FbaI | TGATCA | 1 cut(s) | 76 |
| FspBI | CTAG | 4 cut(s) | 6, 44, 56, 230 |
| GsuI | CTGGAG | 1 cut(s) | 192 |
| HaeIII | GGCC | 1 cut(s) | 335 |
| Hin1II | CATG | 2 cut(s) | 373, 377 |
| Hpy166II | GTNNAC | 1 cut(s) | 151 |
| Hpy188I | TCNGA | 1 cut(s) | 312 |
| Hpy188III | TCNNGA | 2 cut(s) | 44, 56 |
| Hpy8I | GTNNAC | 1 cut(s) | 151 |
| HpyAV | CCTTC | 1 cut(s) | 213 |
| HpyCH4V | TGCA | 1 cut(s) | 373 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 10 |
| HpyF3I | CTNAG | 1 cut(s) | 311 |
| Hsp92II | CATG | 2 cut(s) | 373, 377 |
| Ksp22I | TGATCA | 1 cut(s) | 76 |
| Kzo9I | GATC | 3 cut(s) | 76, 139, 298 |
| LpnPI | CCDG | 8 cut(s) | 19, 107, 131, 150, 156, 211, 226, 290 |
| LweI | GCATC | 1 cut(s) | 39 |
| MaeI | CTAG | 4 cut(s) | 6, 44, 56, 230 |
| MalI | GATC | 3 cut(s) | 78, 141, 300 |
| MboI | GATC | 3 cut(s) | 76, 139, 298 |
| MboII | GAAGA | 4 cut(s) | 134, 245, 308, 331 |
| MflI | RGATCY | 2 cut(s) | 139, 298 |
| MluCI | AATT | 5 cut(s) | 15, 125, 237, 244, 360 |
| MnlI | CCTC | 2 cut(s) | 115, 209 |
| Mph1103I | ATGCAT | 1 cut(s) | 375 |
| MroXI | GAANNNNTTC | 1 cut(s) | 237 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 10 |
| NdeII | GATC | 3 cut(s) | 76, 139, 298 |
| NlaIII | CATG | 2 cut(s) | 373, 377 |
| NsiI | ATGCAT | 1 cut(s) | 375 |
| NspI | RCATGY | 1 cut(s) | 373 |
| PdmI | GAANNNNTTC | 1 cut(s) | 237 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 272, 304 |
| PsuI | RGATCY | 2 cut(s) | 139, 298 |
| RsaI | GTAC | 4 cut(s) | 152, 188, 273, 281 |
| RsaNI | GTAC | 4 cut(s) | 151, 187, 272, 280 |
| Sau3AI | GATC | 3 cut(s) | 76, 139, 298 |
| ScaI | AGTACT | 1 cut(s) | 188 |
| SetI | ASST | 4 cut(s) | 55, 73, 150, 181 |
| SfaNI | GCATC | 1 cut(s) | 39 |
| Sse9I | AATT | 5 cut(s) | 15, 125, 237, 244, 360 |
| SspMI | CTAG | 4 cut(s) | 6, 44, 56, 230 |
| StyI | CCWWGG | 1 cut(s) | 118 |
| TasI | AATT | 5 cut(s) | 15, 125, 237, 244, 360 |
| TatI | WGTACW | 3 cut(s) | 150, 186, 279 |
| TscAI | CASTG | 1 cut(s) | 175 |
| TspDTI | ATGAA | 2 cut(s) | 35, 171 |
| TspGWI | ACGGA | 1 cut(s) | 246 |
| TspRI | CASTG | 1 cut(s) | 175 |
| XapI | RAATTY | 4 cut(s) | 125, 237, 244, 360 |
| XbaI | TCTAGA | 2 cut(s) | 43, 55 |
| XceI | RCATGY | 1 cut(s) | 373 |
| XmnI | GAANNNNTTC | 1 cut(s) | 237 |
| XspI | CTAG | 4 cut(s) | 6, 44, 56, 230 |
| ZrmI | AGTACT | 1 cut(s) | 188 |
| Zsp2I | ATGCAT | 1 cut(s) | 375 |