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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 409 |
| AccI | GTMKAC | 2 cut(s) | 360, 423 |
| AcsI | RAATTY | 4 cut(s) | 95, 101, 244, 398 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 373 |
| AgsI | TTSAA | 3 cut(s) | 100, 143, 404 |
| AluBI | AGCT | 3 cut(s) | 11, 19, 41 |
| AluI | AGCT | 3 cut(s) | 11, 19, 41 |
| AoxI | GGCC | 1 cut(s) | 375 |
| ApoI | RAATTY | 4 cut(s) | 95, 101, 244, 398 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 95 |
| AspS9I | GGNCC | 1 cut(s) | 162 |
| AvaII | GGWCC | 1 cut(s) | 162 |
| BccI | CCATC | 1 cut(s) | 367 |
| BfaI | CTAG | 3 cut(s) | 279, 317, 329 |
| BfuAI | ACCTGC | 1 cut(s) | 409 |
| BglII | AGATCT | 1 cut(s) | 313 |
| Bme18I | GGWCC | 1 cut(s) | 162 |
| BmgT120I | GGNCC | 1 cut(s) | 162 |
| BmsI | GCATC | 1 cut(s) | 312 |
| BoxI | GACNNNNGTC | 1 cut(s) | 324 |
| Bsa29I | ATCGAT | 1 cut(s) | 64 |
| BsaJI | CCNNGG | 2 cut(s) | 222, 391 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 373 |
| Bse1I | ACTGG | 1 cut(s) | 448 |
| BseCI | ATCGAT | 1 cut(s) | 64 |
| BseDI | CCNNGG | 2 cut(s) | 222, 391 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 373 |
| BseMII | CTCAG | 1 cut(s) | 33 |
| BseNI | ACTGG | 1 cut(s) | 448 |
| BsgI | GTGCAG | 1 cut(s) | 366 |
| BshFI | GGCC | 1 cut(s) | 377 |
| BshVI | ATCGAT | 1 cut(s) | 64 |
| BslFI | GGGAC | 1 cut(s) | 245 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 373 |
| BsmFI | GGGAC | 1 cut(s) | 245 |
| BsnI | GGCC | 1 cut(s) | 377 |
| Bsp143I | GATC | 4 cut(s) | 25, 61, 288, 313 |
| BspANI | GGCC | 1 cut(s) | 377 |
| BspCNI | CTCAG | 2 cut(s) | 4, 34 |
| BspDI | ATCGAT | 1 cut(s) | 64 |
| BspMI | ACCTGC | 1 cut(s) | 409 |
| BsrI | ACTGG | 1 cut(s) | 448 |
| BssECI | CCNNGG | 2 cut(s) | 222, 391 |
| BssMI | GATC | 4 cut(s) | 25, 61, 288, 313 |
| BssNAI | GTATAC | 1 cut(s) | 424 |
| BssT1I | CCWWGG | 2 cut(s) | 222, 391 |
| Bst1107I | GTATAC | 1 cut(s) | 424 |
| BstDEI | CTNAG | 2 cut(s) | 12, 42 |
| BstKTI | GATC | 4 cut(s) | 28, 64, 291, 316 |
| BstMBI | GATC | 4 cut(s) | 25, 61, 288, 313 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 324 |
| BstX2I | RGATCY | 1 cut(s) | 313 |
| BstYI | RGATCY | 1 cut(s) | 313 |
| BstZ17I | GTATAC | 1 cut(s) | 424 |
| Bsu15I | ATCGAT | 1 cut(s) | 64 |
| BsuRI | GGCC | 1 cut(s) | 377 |
| BsuTUI | ATCGAT | 1 cut(s) | 64 |
| BveI | ACCTGC | 1 cut(s) | 409 |
| Cfr13I | GGNCC | 1 cut(s) | 162 |
| ClaI | ATCGAT | 1 cut(s) | 64 |
| CviJI | RGCY | 6 cut(s) | 11, 19, 41, 218, 366, 377 |
| CviKI_1 | RGCY | 6 cut(s) | 11, 19, 41, 218, 366, 377 |
| DdeI | CTNAG | 2 cut(s) | 12, 42 |
| DpnI | GATC | 4 cut(s) | 27, 63, 290, 315 |
| DpnII | GATC | 4 cut(s) | 25, 61, 288, 313 |
| Eco130I | CCWWGG | 2 cut(s) | 222, 391 |
| Eco47I | GGWCC | 1 cut(s) | 162 |
| EcoRI | GAATTC | 1 cut(s) | 95 |
| EcoT14I | CCWWGG | 2 cut(s) | 222, 391 |
| ErhI | CCWWGG | 2 cut(s) | 222, 391 |
| FaiI | YATR | 5 cut(s) | 24, 33, 300, 424, 429 |
| FaqI | GGGAC | 1 cut(s) | 245 |
| FblI | GTMKAC | 2 cut(s) | 360, 423 |
| FspBI | CTAG | 3 cut(s) | 279, 317, 329 |
| HaeIII | GGCC | 1 cut(s) | 377 |
| HinfI | GANTC | 1 cut(s) | 325 |
| Hpy166II | GTNNAC | 2 cut(s) | 361, 424 |
| Hpy188I | TCNGA | 4 cut(s) | 8, 107, 213, 288 |
| Hpy188III | TCNNGA | 6 cut(s) | 59, 134, 165, 292, 317, 329 |
| Hpy8I | GTNNAC | 2 cut(s) | 361, 424 |
| HpyAV | CCTTC | 1 cut(s) | 146 |
| HpyCH4V | TGCA | 2 cut(s) | 383, 418 |
| HpyF3I | CTNAG | 2 cut(s) | 12, 42 |
| Kzo9I | GATC | 4 cut(s) | 25, 61, 288, 313 |
| LpnPI | CCDG | 8 cut(s) | 72, 178, 276, 292, 380, 404, 423, 429 |
| LweI | GCATC | 1 cut(s) | 312 |
| MaeI | CTAG | 3 cut(s) | 279, 317, 329 |
| MalI | GATC | 4 cut(s) | 27, 63, 290, 315 |
| MboI | GATC | 4 cut(s) | 25, 61, 288, 313 |
| MboII | GAAGA | 3 cut(s) | 47, 95, 161 |
| MflI | RGATCY | 1 cut(s) | 313 |
| MluCI | AATT | 5 cut(s) | 46, 95, 101, 244, 398 |
| MlyI | GAGTC | 1 cut(s) | 334 |
| MnlI | CCTC | 2 cut(s) | 259, 388 |
| MroXI | GAANNNNTTC | 1 cut(s) | 95 |
| MseI | TTAA | 1 cut(s) | 68 |
| NdeII | GATC | 4 cut(s) | 25, 61, 288, 313 |
| PdmI | GAANNNNTTC | 1 cut(s) | 95 |
| PleI | GAGTC | 1 cut(s) | 333 |
| PpsI | GAGTC | 1 cut(s) | 333 |
| PshAI | GACNNNNGTC | 1 cut(s) | 324 |
| PspPI | GGNCC | 1 cut(s) | 162 |
| PsuI | RGATCY | 1 cut(s) | 313 |
| SaqAI | TTAA | 1 cut(s) | 68 |
| Sau3AI | GATC | 4 cut(s) | 25, 61, 288, 313 |
| Sau96I | GGNCC | 1 cut(s) | 162 |
| SchI | GAGTC | 1 cut(s) | 334 |
| SetI | ASST | 5 cut(s) | 13, 21, 43, 164, 423 |
| SfaNI | GCATC | 1 cut(s) | 312 |
| SinI | GGWCC | 1 cut(s) | 162 |
| Sse9I | AATT | 5 cut(s) | 46, 95, 101, 244, 398 |
| SspMI | CTAG | 3 cut(s) | 279, 317, 329 |
| StyI | CCWWGG | 2 cut(s) | 222, 391 |
| TaqI | TCGA | 2 cut(s) | 64, 81 |
| TasI | AATT | 5 cut(s) | 46, 95, 101, 244, 398 |
| Tru1I | TTAA | 1 cut(s) | 68 |
| Tru9I | TTAA | 1 cut(s) | 68 |
| TspDTI | ATGAA | 4 cut(s) | 48, 104, 163, 444 |
| VpaK11BI | GGWCC | 1 cut(s) | 162 |
| XapI | RAATTY | 4 cut(s) | 95, 101, 244, 398 |
| XbaI | TCTAGA | 2 cut(s) | 316, 328 |
| XmiI | GTMKAC | 2 cut(s) | 360, 423 |
| XmnI | GAANNNNTTC | 1 cut(s) | 95 |
| XspI | CTAG | 3 cut(s) | 279, 317, 329 |