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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 216 |
| AcoI | YGGCCR | 1 cut(s) | 516 |
| AcsI | RAATTY | 2 cut(s) | 100, 254 |
| AcuI | CTGAAG | 1 cut(s) | 311 |
| AfaI | GTAC | 4 cut(s) | 141, 281, 335, 456 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 229 |
| AgsI | TTSAA | 5 cut(s) | 260, 275, 287, 341, 385 |
| AluBI | AGCT | 5 cut(s) | 18, 182, 263, 317, 395 |
| AluI | AGCT | 5 cut(s) | 18, 182, 263, 317, 395 |
| Alw26I | GTCTC | 1 cut(s) | 383 |
| AoxI | GGCC | 1 cut(s) | 516 |
| ApoI | RAATTY | 2 cut(s) | 100, 254 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 345 |
| AsuC2I | CCSGG | 1 cut(s) | 301 |
| AsuHPI | GGTGA | 2 cut(s) | 289, 343 |
| AsuII | TTCGAA | 1 cut(s) | 431 |
| BccI | CCATC | 1 cut(s) | 223 |
| BceAI | ACGGC | 1 cut(s) | 531 |
| BclI | TGATCA | 1 cut(s) | 466 |
| BcnI | CCSGG | 1 cut(s) | 301 |
| BcoDI | GTCTC | 1 cut(s) | 383 |
| BfaI | CTAG | 4 cut(s) | 167, 173, 185, 396 |
| BglII | AGATCT | 1 cut(s) | 481 |
| Bme1390I | CCNGG | 1 cut(s) | 301 |
| BmrFI | CCNGG | 1 cut(s) | 301 |
| BmsI | GCATC | 1 cut(s) | 242 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 184, 216 |
| Bpu14I | TTCGAA | 1 cut(s) | 431 |
| BpuMI | CCSGG | 1 cut(s) | 301 |
| BsaJI | CCNNGG | 2 cut(s) | 247, 300 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 42, 72 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 229 |
| BseDI | CCNNGG | 2 cut(s) | 247, 300 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 229 |
| BshFI | GGCC | 1 cut(s) | 518 |
| BsiSI | CCGG | 1 cut(s) | 300 |
| BslFI | GGGAC | 1 cut(s) | 101 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 229 |
| BsmAI | GTCTC | 1 cut(s) | 383 |
| BsmFI | GGGAC | 1 cut(s) | 101 |
| BsnI | GGCC | 1 cut(s) | 518 |
| Bsp119I | TTCGAA | 1 cut(s) | 431 |
| Bsp143I | GATC | 4 cut(s) | 268, 322, 466, 481 |
| BspANI | GGCC | 1 cut(s) | 518 |
| BspT104I | TTCGAA | 1 cut(s) | 431 |
| BssECI | CCNNGG | 2 cut(s) | 247, 300 |
| BssMI | GATC | 4 cut(s) | 268, 322, 466, 481 |
| BssT1I | CCWWGG | 1 cut(s) | 247 |
| Bst4CI | ACNGT | 2 cut(s) | 158, 454 |
| BstBI | TTCGAA | 1 cut(s) | 431 |
| BstKTI | GATC | 4 cut(s) | 271, 325, 469, 484 |
| BstMAI | GTCTC | 1 cut(s) | 383 |
| BstMBI | GATC | 4 cut(s) | 268, 322, 466, 481 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 314 |
| BstSCI | CCNGG | 1 cut(s) | 299 |
| BstX2I | RGATCY | 1 cut(s) | 481 |
| BstYI | RGATCY | 1 cut(s) | 481 |
| BsuRI | GGCC | 1 cut(s) | 518 |
| Csp6I | GTAC | 4 cut(s) | 140, 280, 334, 455 |
| CviQI | GTAC | 4 cut(s) | 140, 280, 334, 455 |
| DpnI | GATC | 4 cut(s) | 270, 324, 468, 483 |
| DpnII | GATC | 4 cut(s) | 268, 322, 466, 481 |
| EaeI | YGGCCR | 1 cut(s) | 516 |
| Eco130I | CCWWGG | 1 cut(s) | 247 |
| Eco57I | CTGAAG | 1 cut(s) | 311 |
| EcoT14I | CCWWGG | 1 cut(s) | 247 |
| ErhI | CCWWGG | 1 cut(s) | 247 |
| FaiI | YATR | 3 cut(s) | 444, 465, 494 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 359, 391 |
| FaqI | GGGAC | 1 cut(s) | 101 |
| FbaI | TGATCA | 1 cut(s) | 466 |
| FblI | GTMKAC | 1 cut(s) | 216 |
| FspBI | CTAG | 4 cut(s) | 167, 173, 185, 396 |
| HaeIII | GGCC | 1 cut(s) | 518 |
| HapII | CCGG | 1 cut(s) | 300 |
| HpaII | CCGG | 1 cut(s) | 300 |
| HphI | GGTGA | 2 cut(s) | 289, 343 |
| Hpy166II | GTNNAC | 1 cut(s) | 217 |
| Hpy188I | TCNGA | 1 cut(s) | 330 |
| Hpy188III | TCNNGA | 4 cut(s) | 185, 203, 266, 320 |
| Hpy8I | GTNNAC | 1 cut(s) | 217 |
| HpyCH4III | ACNGT | 2 cut(s) | 158, 454 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 314 |
| Ksp22I | TGATCA | 1 cut(s) | 466 |
| Kzo9I | GATC | 4 cut(s) | 268, 322, 466, 481 |
| LmnI | GCTCC | 3 cut(s) | 23, 268, 322 |
| LpnPI | CCDG | 9 cut(s) | 132, 148, 236, 279, 313, 333, 367, 398, 473 |
| LweI | GCATC | 1 cut(s) | 242 |
| MaeI | CTAG | 4 cut(s) | 167, 173, 185, 396 |
| MalI | GATC | 4 cut(s) | 270, 324, 468, 483 |
| MboI | GATC | 4 cut(s) | 268, 322, 466, 481 |
| MboII | GAAGA | 4 cut(s) | 17, 263, 317, 428 |
| MflI | RGATCY | 1 cut(s) | 481 |
| MluCI | AATT | 4 cut(s) | 100, 144, 151, 254 |
| MnlI | CCTC | 2 cut(s) | 115, 392 |
| MroXI | GAANNNNTTC | 1 cut(s) | 345 |
| MspI | CCGG | 1 cut(s) | 300 |
| MspR9I | CCNGG | 1 cut(s) | 301 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 314 |
| NciI | CCSGG | 1 cut(s) | 301 |
| NdeII | GATC | 4 cut(s) | 268, 322, 466, 481 |
| NspV | TTCGAA | 1 cut(s) | 431 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 437 |
| PdmI | GAANNNNTTC | 1 cut(s) | 345 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 455, 487 |
| PsuI | RGATCY | 1 cut(s) | 481 |
| RsaI | GTAC | 4 cut(s) | 141, 281, 335, 456 |
| RsaNI | GTAC | 4 cut(s) | 140, 280, 334, 455 |
| Sau3AI | GATC | 4 cut(s) | 268, 322, 466, 481 |
| ScrFI | CCNGG | 1 cut(s) | 301 |
| SetI | ASST | 6 cut(s) | 20, 174, 184, 265, 319, 397 |
| SfaNI | GCATC | 1 cut(s) | 242 |
| SfuI | TTCGAA | 1 cut(s) | 431 |
| Sse9I | AATT | 4 cut(s) | 100, 144, 151, 254 |
| SspMI | CTAG | 4 cut(s) | 167, 173, 185, 396 |
| StyD4I | CCNGG | 1 cut(s) | 299 |
| StyI | CCWWGG | 1 cut(s) | 247 |
| TaaI | ACNGT | 2 cut(s) | 158, 454 |
| TaqI | TCGA | 1 cut(s) | 431 |
| TasI | AATT | 4 cut(s) | 100, 144, 151, 254 |
| TatI | WGTACW | 2 cut(s) | 279, 333 |
| TspDTI | ATGAA | 1 cut(s) | 19 |
| TspGWI | ACGGA | 1 cut(s) | 429 |
| XapI | RAATTY | 2 cut(s) | 100, 254 |
| XbaI | TCTAGA | 1 cut(s) | 184 |
| XmiI | GTMKAC | 1 cut(s) | 216 |
| XmnI | GAANNNNTTC | 1 cut(s) | 345 |
| XspI | CTAG | 4 cut(s) | 167, 173, 185, 396 |