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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 3 cut(s) | 100, 159, 254 |
| AcuI | CTGAAG | 1 cut(s) | 257 |
| AfaI | GTAC | 4 cut(s) | 66, 141, 281, 402 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 229 |
| AgsI | TTSAA | 3 cut(s) | 260, 287, 331 |
| AluBI | AGCT | 4 cut(s) | 18, 52, 182, 263 |
| AluI | AGCT | 4 cut(s) | 18, 52, 182, 263 |
| AoxI | GGCC | 2 cut(s) | 72, 231 |
| ApoI | RAATTY | 3 cut(s) | 100, 159, 254 |
| AspS9I | GGNCC | 1 cut(s) | 73 |
| AsuHPI | GGTGA | 1 cut(s) | 289 |
| AsuII | TTCGAA | 1 cut(s) | 377 |
| AxyI | CCTNAGG | 1 cut(s) | 78 |
| BccI | CCATC | 2 cut(s) | 38, 223 |
| BfaI | CTAG | 4 cut(s) | 135, 167, 185, 215 |
| BglII | AGATCT | 1 cut(s) | 169 |
| BmgT120I | GGNCC | 1 cut(s) | 73 |
| BmiI | GGNNCC | 1 cut(s) | 75 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 184, 216 |
| Bpu14I | TTCGAA | 1 cut(s) | 377 |
| BsaJI | CCNNGG | 1 cut(s) | 247 |
| BsaWI | WCCGGW | 1 cut(s) | 299 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 229 |
| Bse21I | CCTNAGG | 1 cut(s) | 78 |
| BseDI | CCNNGG | 1 cut(s) | 247 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 229 |
| BshFI | GGCC | 2 cut(s) | 74, 233 |
| BsiSI | CCGG | 1 cut(s) | 300 |
| BslFI | GGGAC | 1 cut(s) | 101 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 229 |
| BsmFI | GGGAC | 1 cut(s) | 101 |
| BsnI | GGCC | 2 cut(s) | 74, 233 |
| Bsp119I | TTCGAA | 1 cut(s) | 377 |
| Bsp1407I | TGTACA | 1 cut(s) | 64 |
| Bsp143I | GATC | 2 cut(s) | 169, 268 |
| BspANI | GGCC | 2 cut(s) | 74, 233 |
| BspLI | GGNNCC | 1 cut(s) | 75 |
| BspT104I | TTCGAA | 1 cut(s) | 377 |
| BsrGI | TGTACA | 1 cut(s) | 64 |
| BssECI | CCNNGG | 1 cut(s) | 247 |
| BssMI | GATC | 2 cut(s) | 169, 268 |
| BssT1I | CCWWGG | 1 cut(s) | 247 |
| BstAUI | TGTACA | 1 cut(s) | 64 |
| BstBI | TTCGAA | 1 cut(s) | 377 |
| BstDEI | CTNAG | 1 cut(s) | 78 |
| BstKTI | GATC | 2 cut(s) | 172, 271 |
| BstMBI | GATC | 2 cut(s) | 169, 268 |
| BstX2I | RGATCY | 1 cut(s) | 169 |
| BstYI | RGATCY | 1 cut(s) | 169 |
| Bsu36I | CCTNAGG | 1 cut(s) | 78 |
| BsuRI | GGCC | 2 cut(s) | 74, 233 |
| Cfr13I | GGNCC | 1 cut(s) | 73 |
| Csp6I | GTAC | 4 cut(s) | 65, 140, 280, 401 |
| CviQI | GTAC | 4 cut(s) | 65, 140, 280, 401 |
| DdeI | CTNAG | 1 cut(s) | 78 |
| DpnI | GATC | 2 cut(s) | 171, 270 |
| DpnII | GATC | 2 cut(s) | 169, 268 |
| Eco130I | CCWWGG | 1 cut(s) | 247 |
| Eco57I | CTGAAG | 1 cut(s) | 257 |
| Eco81I | CCTNAGG | 1 cut(s) | 78 |
| EcoO109I | RGGNCCY | 1 cut(s) | 73 |
| EcoT14I | CCWWGG | 1 cut(s) | 247 |
| ErhI | CCWWGG | 1 cut(s) | 247 |
| FaiI | YATR | 3 cut(s) | 156, 344, 390 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 305, 337 |
| FaqI | GGGAC | 1 cut(s) | 101 |
| FspBI | CTAG | 4 cut(s) | 135, 167, 185, 215 |
| HaeIII | GGCC | 2 cut(s) | 74, 233 |
| HapII | CCGG | 1 cut(s) | 300 |
| HpaII | CCGG | 1 cut(s) | 300 |
| HphI | GGTGA | 1 cut(s) | 289 |
| Hpy188I | TCNGA | 1 cut(s) | 276 |
| Hpy188III | TCNNGA | 4 cut(s) | 173, 185, 203, 266 |
| HpyAV | CCTTC | 1 cut(s) | 64 |
| HpyCH4V | TGCA | 1 cut(s) | 398 |
| HpyF3I | CTNAG | 1 cut(s) | 78 |
| Kzo9I | GATC | 2 cut(s) | 169, 268 |
| LmnI | GCTCC | 2 cut(s) | 23, 268 |
| LpnPI | CCDG | 6 cut(s) | 75, 132, 158, 236, 279, 313 |
| MaeI | CTAG | 4 cut(s) | 135, 167, 185, 215 |
| MaeIII | GTNAC | 1 cut(s) | 129 |
| MalI | GATC | 2 cut(s) | 171, 270 |
| MboI | GATC | 2 cut(s) | 169, 268 |
| MboII | GAAGA | 3 cut(s) | 17, 263, 374 |
| MflI | RGATCY | 1 cut(s) | 169 |
| MluCI | AATT | 4 cut(s) | 100, 144, 159, 254 |
| MmeI | TCCRAC | 1 cut(s) | 174 |
| MnlI | CCTC | 2 cut(s) | 115, 244 |
| MspI | CCGG | 1 cut(s) | 300 |
| NdeII | GATC | 2 cut(s) | 169, 268 |
| NlaIV | GGNNCC | 1 cut(s) | 75 |
| NspV | TTCGAA | 1 cut(s) | 377 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 383 |
| PspN4I | GGNNCC | 1 cut(s) | 75 |
| PspPI | GGNCC | 1 cut(s) | 73 |
| PsuI | RGATCY | 1 cut(s) | 169 |
| RsaI | GTAC | 4 cut(s) | 66, 141, 281, 402 |
| RsaNI | GTAC | 4 cut(s) | 65, 140, 280, 401 |
| Sau3AI | GATC | 2 cut(s) | 169, 268 |
| Sau96I | GGNCC | 1 cut(s) | 73 |
| SetI | ASST | 5 cut(s) | 20, 54, 136, 184, 265 |
| SfuI | TTCGAA | 1 cut(s) | 377 |
| Sse9I | AATT | 4 cut(s) | 100, 144, 159, 254 |
| SspI | AATATT | 1 cut(s) | 111 |
| SspMI | CTAG | 4 cut(s) | 135, 167, 185, 215 |
| StyI | CCWWGG | 1 cut(s) | 247 |
| TaqI | TCGA | 1 cut(s) | 377 |
| TasI | AATT | 4 cut(s) | 100, 144, 159, 254 |
| TatI | WGTACW | 2 cut(s) | 64, 279 |
| TspGWI | ACGGA | 1 cut(s) | 375 |
| XapI | RAATTY | 3 cut(s) | 100, 159, 254 |
| XbaI | TCTAGA | 1 cut(s) | 184 |
| XspI | CTAG | 4 cut(s) | 135, 167, 185, 215 |