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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 409 |
| Acc36I | ACCTGC | 1 cut(s) | 409 |
| AccI | GTMKAC | 1 cut(s) | 360 |
| AcsI | RAATTY | 3 cut(s) | 95, 244, 398 |
| AfaI | GTAC | 2 cut(s) | 425, 461 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 82, 373 |
| AgsI | TTSAA | 4 cut(s) | 100, 143, 404, 475 |
| AleI | CACNNNNGTG | 1 cut(s) | 446 |
| AluBI | AGCT | 4 cut(s) | 11, 19, 41, 162 |
| AluI | AGCT | 4 cut(s) | 11, 19, 41, 162 |
| Alw26I | GTCTC | 1 cut(s) | 489 |
| Ama87I | CYCGRG | 1 cut(s) | 263 |
| AoxI | GGCC | 2 cut(s) | 375, 500 |
| ApoI | RAATTY | 3 cut(s) | 95, 244, 398 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 95 |
| AspS9I | GGNCC | 1 cut(s) | 501 |
| AsuHPI | GGTGA | 2 cut(s) | 283, 433 |
| AvaI | CYCGRG | 1 cut(s) | 263 |
| BccI | CCATC | 1 cut(s) | 367 |
| BcoDI | GTCTC | 1 cut(s) | 489 |
| BfaI | CTAG | 2 cut(s) | 317, 329 |
| BfuAI | ACCTGC | 1 cut(s) | 409 |
| BmcAI | AGTACT | 1 cut(s) | 461 |
| BmeT110I | CYCGRG | 1 cut(s) | 263 |
| BmgT120I | GGNCC | 1 cut(s) | 501 |
| BmrI | ACTGGG | 1 cut(s) | 200 |
| BmuI | ACTGGG | 1 cut(s) | 200 |
| BoxI | GACNNNNGTC | 1 cut(s) | 324 |
| BpmI | CTGGAG | 1 cut(s) | 148 |
| BsaJI | CCNNGG | 4 cut(s) | 222, 264, 391, 520 |
| BsaWI | WCCGGW | 1 cut(s) | 443 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 82, 373 |
| Bse1I | ACTGG | 2 cut(s) | 206, 503 |
| BseDI | CCNNGG | 4 cut(s) | 222, 264, 391, 520 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 82, 373 |
| BseMII | CTCAG | 2 cut(s) | 19, 33 |
| BseNI | ACTGG | 2 cut(s) | 206, 503 |
| BsgI | GTGCAG | 1 cut(s) | 366 |
| BshFI | GGCC | 2 cut(s) | 377, 502 |
| BsiHKCI | CYCGRG | 1 cut(s) | 263 |
| BsiSI | CCGG | 1 cut(s) | 444 |
| BslFI | GGGAC | 1 cut(s) | 245 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 82, 373 |
| BsmAI | GTCTC | 1 cut(s) | 489 |
| BsmFI | GGGAC | 1 cut(s) | 245 |
| BsnI | GGCC | 2 cut(s) | 377, 502 |
| BsoBI | CYCGRG | 1 cut(s) | 263 |
| Bsp143I | GATC | 1 cut(s) | 25 |
| BspANI | GGCC | 2 cut(s) | 377, 502 |
| BspCNI | CTCAG | 3 cut(s) | 4, 18, 34 |
| BspMI | ACCTGC | 1 cut(s) | 409 |
| BsrI | ACTGG | 2 cut(s) | 206, 503 |
| BssECI | CCNNGG | 4 cut(s) | 222, 264, 391, 520 |
| BssMI | GATC | 1 cut(s) | 25 |
| BssT1I | CCWWGG | 1 cut(s) | 391 |
| Bst6I | CTCTTC | 1 cut(s) | 487 |
| BstDEI | CTNAG | 3 cut(s) | 5, 12, 42 |
| BstDSI | CCRYGG | 2 cut(s) | 222, 520 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 80 |
| BstKTI | GATC | 1 cut(s) | 28 |
| BstMAI | GTCTC | 1 cut(s) | 489 |
| BstMBI | GATC | 1 cut(s) | 25 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 324 |
| BsuRI | GGCC | 2 cut(s) | 377, 502 |
| BtgI | CCRYGG | 2 cut(s) | 222, 520 |
| BveI | ACCTGC | 1 cut(s) | 409 |
| Cfr13I | GGNCC | 1 cut(s) | 501 |
| Csp6I | GTAC | 2 cut(s) | 424, 460 |
| CviQI | GTAC | 2 cut(s) | 424, 460 |
| DdeI | CTNAG | 3 cut(s) | 5, 12, 42 |
| DpnI | GATC | 1 cut(s) | 27 |
| DpnII | GATC | 1 cut(s) | 25 |
| Eam1104I | CTCTTC | 1 cut(s) | 487 |
| EarI | CTCTTC | 1 cut(s) | 487 |
| Eco130I | CCWWGG | 1 cut(s) | 391 |
| Eco88I | CYCGRG | 1 cut(s) | 263 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 80 |
| EcoRI | GAATTC | 1 cut(s) | 95 |
| EcoT14I | CCWWGG | 1 cut(s) | 391 |
| ErhI | CCWWGG | 1 cut(s) | 391 |
| FaiI | YATR | 4 cut(s) | 24, 176, 300, 429 |
| FaqI | GGGAC | 1 cut(s) | 245 |
| FblI | GTMKAC | 1 cut(s) | 360 |
| FspBI | CTAG | 2 cut(s) | 317, 329 |
| GsuI | CTGGAG | 1 cut(s) | 148 |
| HaeIII | GGCC | 2 cut(s) | 377, 502 |
| HapII | CCGG | 1 cut(s) | 444 |
| HinfI | GANTC | 4 cut(s) | 255, 303, 313, 325 |
| HpaII | CCGG | 1 cut(s) | 444 |
| HphI | GGTGA | 2 cut(s) | 283, 433 |
| Hpy166II | GTNNAC | 2 cut(s) | 361, 424 |
| Hpy188I | TCNGA | 3 cut(s) | 8, 213, 511 |
| Hpy188III | TCNNGA | 4 cut(s) | 59, 165, 317, 329 |
| Hpy8I | GTNNAC | 2 cut(s) | 361, 424 |
| HpyAV | CCTTC | 2 cut(s) | 146, 481 |
| HpyCH4V | TGCA | 3 cut(s) | 105, 383, 418 |
| HpyF3I | CTNAG | 3 cut(s) | 5, 12, 42 |
| Kzo9I | GATC | 1 cut(s) | 25 |
| LmnI | GCTCC | 1 cut(s) | 167 |
| MaeI | CTAG | 2 cut(s) | 317, 329 |
| MalI | GATC | 1 cut(s) | 27 |
| MboI | GATC | 1 cut(s) | 25 |
| MboII | GAAGA | 2 cut(s) | 161, 504 |
| MluCI | AATT | 5 cut(s) | 46, 95, 244, 398, 505 |
| MlyI | GAGTC | 1 cut(s) | 334 |
| MnlI | CCTC | 5 cut(s) | 76, 149, 259, 388, 482 |
| MroXI | GAANNNNTTC | 1 cut(s) | 95 |
| MslI | CAYNNNNRTG | 1 cut(s) | 446 |
| MspI | CCGG | 1 cut(s) | 444 |
| NdeII | GATC | 1 cut(s) | 25 |
| OliI | CACNNNNGTG | 1 cut(s) | 446 |
| PaqCI | CACCTGC | 1 cut(s) | 409 |
| PdmI | GAANNNNTTC | 1 cut(s) | 95 |
| PfeI | GAWTC | 3 cut(s) | 255, 303, 313 |
| PleI | GAGTC | 1 cut(s) | 333 |
| PpsI | GAGTC | 1 cut(s) | 333 |
| PshAI | GACNNNNGTC | 1 cut(s) | 324 |
| PspPI | GGNCC | 1 cut(s) | 501 |
| RsaI | GTAC | 2 cut(s) | 425, 461 |
| RsaNI | GTAC | 2 cut(s) | 424, 460 |
| RseI | CAYNNNNRTG | 1 cut(s) | 446 |
| Sau3AI | GATC | 1 cut(s) | 25 |
| Sau96I | GGNCC | 1 cut(s) | 501 |
| ScaI | AGTACT | 1 cut(s) | 461 |
| SchI | GAGTC | 1 cut(s) | 334 |
| SetI | ASST | 7 cut(s) | 13, 21, 43, 78, 164, 423, 468 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 446 |
| Sse9I | AATT | 5 cut(s) | 46, 95, 244, 398, 505 |
| SspMI | CTAG | 2 cut(s) | 317, 329 |
| StyI | CCWWGG | 1 cut(s) | 391 |
| TaqI | TCGA | 1 cut(s) | 81 |
| TasI | AATT | 5 cut(s) | 46, 95, 244, 398, 505 |
| TatI | WGTACW | 2 cut(s) | 423, 459 |
| TfiI | GAWTC | 3 cut(s) | 255, 303, 313 |
| TspDTI | ATGAA | 4 cut(s) | 104, 163, 212, 444 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 80 |
| XapI | RAATTY | 3 cut(s) | 95, 244, 398 |
| XbaI | TCTAGA | 2 cut(s) | 316, 328 |
| XmiI | GTMKAC | 1 cut(s) | 360 |
| XmnI | GAANNNNTTC | 1 cut(s) | 95 |
| XspI | CTAG | 2 cut(s) | 317, 329 |
| ZrmI | AGTACT | 1 cut(s) | 461 |