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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 139 |
| AcsI | RAATTY | 1 cut(s) | 37 |
| AfaI | GTAC | 1 cut(s) | 459 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 139 |
| AgsI | TTSAA | 1 cut(s) | 316 |
| AhlI | ACTAGT | 2 cut(s) | 377, 386 |
| AluBI | AGCT | 3 cut(s) | 202, 305, 463 |
| AluI | AGCT | 3 cut(s) | 202, 305, 463 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 76 |
| ApoI | RAATTY | 1 cut(s) | 37 |
| AspLEI | GCGC | 1 cut(s) | 283 |
| AsuC2I | CCSGG | 1 cut(s) | 407 |
| BciVI | GTATCC | 1 cut(s) | 401 |
| BcnI | CCSGG | 1 cut(s) | 407 |
| BcuI | ACTAGT | 2 cut(s) | 377, 386 |
| BfaI | CTAG | 6 cut(s) | 83, 93, 110, 332, 378, 387 |
| BfmI | CTRYAG | 1 cut(s) | 285 |
| BfoI | RGCGCY | 1 cut(s) | 284 |
| BfuI | GTATCC | 1 cut(s) | 401 |
| Bme1390I | CCNGG | 1 cut(s) | 407 |
| BmrFI | CCNGG | 1 cut(s) | 407 |
| BmsI | GCATC | 1 cut(s) | 444 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 447, 479 |
| BpuMI | CCSGG | 1 cut(s) | 407 |
| BsaBI | GATNNNNATC | 1 cut(s) | 339 |
| BsaWI | WCCGGW | 1 cut(s) | 393 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 139 |
| Bse8I | GATNNNNATC | 1 cut(s) | 339 |
| BseJI | GATNNNNATC | 1 cut(s) | 339 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 139 |
| BseRI | GAGGAG | 1 cut(s) | 43 |
| BsiSI | CCGG | 2 cut(s) | 394, 407 |
| BslFI | GGGAC | 1 cut(s) | 19 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 139 |
| BsmFI | GGGAC | 1 cut(s) | 19 |
| Bst4CI | ACNGT | 2 cut(s) | 208, 289 |
| BstH2I | RGCGCY | 1 cut(s) | 284 |
| BstHHI | GCGC | 1 cut(s) | 283 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 241 |
| BstSCI | CCNGG | 1 cut(s) | 405 |
| BstSFI | CTRYAG | 1 cut(s) | 285 |
| BsuI | GTATCC | 1 cut(s) | 401 |
| CaiI | CAGNNNCTG | 1 cut(s) | 76 |
| CfoI | GCGC | 1 cut(s) | 283 |
| Csp6I | GTAC | 1 cut(s) | 458 |
| CviAII | CATG | 1 cut(s) | 355 |
| CviJI | RGCY | 3 cut(s) | 202, 305, 463 |
| CviKI_1 | RGCY | 3 cut(s) | 202, 305, 463 |
| CviQI | GTAC | 1 cut(s) | 458 |
| Eco32I | GATATC | 1 cut(s) | 421 |
| EcoRV | GATATC | 1 cut(s) | 421 |
| FaeI | CATG | 1 cut(s) | 358 |
| FaiI | YATR | 7 cut(s) | 128, 168, 254, 263, 356, 384, 399 |
| FaqI | GGGAC | 1 cut(s) | 19 |
| FatI | CATG | 1 cut(s) | 354 |
| FspBI | CTAG | 6 cut(s) | 83, 93, 110, 332, 378, 387 |
| GlaI | GCGC | 1 cut(s) | 282 |
| HaeII | RGCGCY | 1 cut(s) | 284 |
| HapII | CCGG | 2 cut(s) | 394, 407 |
| HhaI | GCGC | 1 cut(s) | 283 |
| Hin1II | CATG | 1 cut(s) | 358 |
| Hin6I | GCGC | 1 cut(s) | 281 |
| HinP1I | GCGC | 1 cut(s) | 281 |
| HinfI | GANTC | 4 cut(s) | 73, 248, 312, 340 |
| HpaII | CCGG | 2 cut(s) | 394, 407 |
| Hpy188I | TCNGA | 4 cut(s) | 16, 58, 72, 443 |
| Hpy188III | TCNNGA | 3 cut(s) | 93, 110, 424 |
| HpyCH4III | ACNGT | 2 cut(s) | 208, 289 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 241 |
| Hsp92II | CATG | 1 cut(s) | 358 |
| HspAI | GCGC | 1 cut(s) | 281 |
| LmnI | GCTCC | 1 cut(s) | 468 |
| LpnPI | CCDG | 3 cut(s) | 230, 407, 420 |
| LweI | GCATC | 1 cut(s) | 444 |
| MaeI | CTAG | 6 cut(s) | 83, 93, 110, 332, 378, 387 |
| MaeIII | GTNAC | 2 cut(s) | 78, 117 |
| MboII | GAAGA | 3 cut(s) | 203, 206, 430 |
| MfeI | CAATTG | 1 cut(s) | 137 |
| MluCI | AATT | 5 cut(s) | 17, 32, 37, 86, 137 |
| MlyI | GAGTC | 1 cut(s) | 82 |
| MnlI | CCTC | 1 cut(s) | 64 |
| MseI | TTAA | 3 cut(s) | 35, 47, 431 |
| MspI | CCGG | 2 cut(s) | 394, 407 |
| MspR9I | CCNGG | 1 cut(s) | 407 |
| MunI | CAATTG | 1 cut(s) | 137 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 241 |
| NciI | CCSGG | 1 cut(s) | 407 |
| NlaIII | CATG | 1 cut(s) | 358 |
| PfeI | GAWTC | 3 cut(s) | 248, 312, 340 |
| PflFI | GACNNNGTC | 1 cut(s) | 10 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 139 |
| PleI | GAGTC | 1 cut(s) | 81 |
| PpsI | GAGTC | 1 cut(s) | 81 |
| PstNI | CAGNNNCTG | 1 cut(s) | 76 |
| PsyI | GACNNNGTC | 1 cut(s) | 10 |
| RsaI | GTAC | 1 cut(s) | 459 |
| RsaNI | GTAC | 1 cut(s) | 458 |
| SaqAI | TTAA | 3 cut(s) | 35, 47, 431 |
| SchI | GAGTC | 1 cut(s) | 82 |
| ScrFI | CCNGG | 1 cut(s) | 407 |
| SetI | ASST | 4 cut(s) | 124, 204, 307, 465 |
| SfaNI | GCATC | 1 cut(s) | 444 |
| SfcI | CTRYAG | 1 cut(s) | 285 |
| SpeI | ACTAGT | 2 cut(s) | 377, 386 |
| Sse9I | AATT | 5 cut(s) | 17, 32, 37, 86, 137 |
| SspMI | CTAG | 6 cut(s) | 83, 93, 110, 332, 378, 387 |
| StyD4I | CCNGG | 1 cut(s) | 405 |
| TaaI | ACNGT | 2 cut(s) | 208, 289 |
| TasI | AATT | 5 cut(s) | 17, 32, 37, 86, 137 |
| TatI | WGTACW | 1 cut(s) | 457 |
| TfiI | GAWTC | 3 cut(s) | 248, 312, 340 |
| Tru1I | TTAA | 3 cut(s) | 35, 47, 431 |
| Tru9I | TTAA | 3 cut(s) | 35, 47, 431 |
| TspDTI | ATGAA | 3 cut(s) | 243, 269, 414 |
| TspGWI | ACGGA | 1 cut(s) | 203 |
| Tth111I | GACNNNGTC | 1 cut(s) | 10 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 139 |
| XapI | RAATTY | 1 cut(s) | 37 |
| XbaI | TCTAGA | 2 cut(s) | 92, 109 |
| XspI | CTAG | 6 cut(s) | 83, 93, 110, 332, 378, 387 |