Rmu_sc0000974.1_g000020

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000974.1
OG0000976
Physical Location & Seq
Forward (+)
95154 .. 96320
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000974.1_g000020.1.cds

Sequence Viewer

Length: 642 bp
atgcctccagtaaaaaagaagactaatgggttcaaaccattagaaaaaccagacacaaatcgtgttccaaacatgatttttctggaaccatctactggtcagactagtatccggttaacagacttccaagctagtctgttggaaacactagaagtatccttatgcgatcaagtggcgtatatcaactacttccccaacttcaccaccagcttgaaagatgcagctcactgtctcagactgatagtcaagacggatgggatatccatgaaggaagatatgcaagaacttgcaataaatgaggtaacttttcctcttgaatggaaattatccggtccaaaaaagcagccggaaagagcaaaagcaaagatctacgagagtagaagaactggagaaatcagcctcaacttcgacaatcaccggaaatgtgatgtctgtcctgagaacctcaggataaacagcagtcttctgagaagaagctacagcactagagaagctagtgtcagtggaacaaaaccccctattgaagagccaatatcagaagaagatctggaagctgatctgaacagaccagtccatatgctcagagctgagaggctctatgatctttatgaagaagccgagaattttgaaaatcctgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

213

Amino Acids

24.45

Weight (kDa)

5.33

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000976)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24112 FvH4_4g10003
rosa_chinensis RchiOBHm_Chr3g0487531 RchiOBHm_Chr5g0034781 RchiOBHm_Chr6g0277631
rosa_laevigata RLG00000021184
rosa_multiflora Rmu_sc0000144.1_g000006 Rmu_sc0000161.1_g000054 Rmu_sc0000383.1_g000008 Rmu_sc0000420.1_g000038 Rmu_sc0000429.1_g000090 Rmu_sc0000429.1_g000091 Rmu_sc0000453.1_g000018 Rmu_sc0000458.1_g000020 Rmu_sc0000483.1_g000003 Rmu_sc0000531.1_g000007 Rmu_sc0000595.1_g000055 Rmu_sc0000768.1_g000022 Rmu_sc0000855.1_g000012 Rmu_sc0000974.1_g000020 Rmu_sc0001018.1_g000020 Rmu_sc0001265.1_g000003 Rmu_sc0001407.1_g000004 Rmu_sc0001694.1_g000007 Rmu_sc0001785.1_g000004 Rmu_sc0001847.1_g000011 Rmu_sc0002148.1_g000003 Rmu_sc0002316.1_g000089 Rmu_sc0002501.1_g000027 Rmu_sc0002521.1_g000031 Rmu_sc0002845.1_g000028 Rmu_sc0003315.1_g000022 Rmu_sc0003371.1_g000015 Rmu_sc0003386.1_g000006 Rmu_sc0003415.1_g000012 Rmu_sc0003467.1_g000010 Rmu_sc0003583.1_g000002 Rmu_sc0003832.1_g000032 Rmu_sc0004094.1_g000040 Rmu_sc0004286.1_g000014 Rmu_sc0004344.1_g000014 Rmu_sc0004798.1_g000005 Rmu_sc0004846.1_g000001 Rmu_sc0005362.1_g000001 Rmu_sc0005481.1_g000002 Rmu_sc0006498.1_g000005 Rmu_sc0006968.1_g000011 Rmu_sc0007369.1_g000004 Rmu_sc0007727.1_g000016 Rmu_sc0008681.1_g000002 Rmu_sc0010278.1_g000009 Rmu_sc0011015.1_g000002 Rmu_sc0011611.1_g000004 Rmu_sc0012216.1_g000005 Rmu_sc0013333.1_g000001 Rmu_sc0016916.1_g000001 Rmu_sc0024220.1_g000001 Rmu_sc0036248.1_g000001 Rmu_sc0037776.1_g000001 Rmu_ssc0000052.1_g000014
rosa_samantha Rh4CG090000
rosa_wichuraiana Rw2G035730 Rw5G039910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AcsI RAATTY 1 cut(s) 622
AfiI CCNNNNNNNGG 1 cut(s) 95
AgsI TTSAA 5 cut(s) 34, 214, 317, 524, 629
AhdI GACNNNNNGTC 1 cut(s) 242
AhlI ACTAGT 1 cut(s) 104
AluBI AGCT 7 cut(s) 131, 210, 224, 477, 494, 554, 587
AluI AGCT 7 cut(s) 131, 210, 224, 477, 494, 554, 587
Alw26I GTCTC 1 cut(s) 236
ApeKI GCWGC 2 cut(s) 221, 343
ApoI RAATTY 1 cut(s) 622
AspS9I GGNCC 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 193, 407
AvaII GGWCC 1 cut(s) 332
AxyI CCTNAGG 1 cut(s) 446
BbsI GAAGAC 2 cut(s) 26, 455
BbvI GCAGC 2 cut(s) 233, 355
BccI CCATC 2 cut(s) 97, 248
BciVI GTATCC 2 cut(s) 119, 166
BcoDI GTCTC 1 cut(s) 236
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 5 cut(s) 105, 132, 149, 486, 495
BfmI CTRYAG 1 cut(s) 478
BfuI GTATCC 2 cut(s) 119, 166
BglII AGATCT 2 cut(s) 366, 544
BisI GCNGC 2 cut(s) 222, 344
BlsI GCNGC 2 cut(s) 223, 345
Bme18I GGWCC 1 cut(s) 332
BmeRI GACNNNNNGTC 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 1 cut(s) 87
BmsI GCATC 1 cut(s) 208
BpiI GAAGAC 2 cut(s) 26, 455
BpmI CTGGAG 1 cut(s) 408
BsaWI WCCGGW 3 cut(s) 111, 329, 417
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse1I ACTGG 4 cut(s) 8, 100, 391, 569
Bse21I CCTNAGG 1 cut(s) 446
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMII CTCAG 6 cut(s) 247, 429, 458, 460, 579, 595
BseNI ACTGG 4 cut(s) 8, 100, 391, 569
BseXI GCAGC 2 cut(s) 233, 355
BsiSI CCGG 4 cut(s) 112, 330, 347, 418
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 1 cut(s) 236
Bsp143I GATC 5 cut(s) 166, 366, 544, 556, 601
BspCNI CTCAG 6 cut(s) 246, 430, 459, 459, 580, 594
BspLI GGNNCC 1 cut(s) 87
BspQI GCTCTTC 1 cut(s) 519
BsrI ACTGG 4 cut(s) 8, 100, 391, 569
BssMI GATC 5 cut(s) 166, 366, 544, 556, 601
Bst4CI ACNGT 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 519
BstDEI CTNAG 6 cut(s) 233, 438, 446, 467, 581, 588
BstF5I GGATG 1 cut(s) 259
BstKTI GATC 5 cut(s) 169, 369, 547, 559, 604
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 5 cut(s) 166, 366, 544, 556, 601
BstSFI CTRYAG 1 cut(s) 478
BstV1I GCAGC 2 cut(s) 233, 355
BstV2I GAAGAC 2 cut(s) 26, 455
BstX2I RGATCY 2 cut(s) 366, 544
BstYI RGATCY 2 cut(s) 366, 544
Bsu36I CCTNAGG 1 cut(s) 446
BsuI GTATCC 2 cut(s) 119, 166
BtsCI GGATG 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 226, 508
Cfr13I GGNCC 1 cut(s) 332
CviAII CATG 2 cut(s) 73, 265
DdeI CTNAG 6 cut(s) 233, 438, 446, 467, 581, 588
DpnI GATC 5 cut(s) 168, 368, 546, 558, 603
DpnII GATC 5 cut(s) 166, 366, 544, 556, 601
DriI GACNNNNNGTC 1 cut(s) 242
Eam1104I CTCTTC 1 cut(s) 519
Eam1105I GACNNNNNGTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 519
Eco32I GATATC 1 cut(s) 261
Eco47I GGWCC 1 cut(s) 332
Eco81I CCTNAGG 1 cut(s) 446
EcoRV GATATC 1 cut(s) 261
FaeI CATG 2 cut(s) 76, 268
FaiI YATR 9 cut(s) 74, 163, 180, 266, 278, 576, 578, 600, 609
FatI CATG 2 cut(s) 72, 264
FauNDI CATATG 1 cut(s) 576
Fnu4HI GCNGC 2 cut(s) 222, 344
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 2 cut(s) 222, 344
FspBI CTAG 5 cut(s) 105, 132, 149, 486, 495
GluI GCNGC 2 cut(s) 222, 344
GsuI CTGGAG 1 cut(s) 408
HapII CCGG 4 cut(s) 112, 330, 347, 418
Hin1II CATG 2 cut(s) 76, 268
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HpaI GTTAAC 1 cut(s) 117
HpaII CCGG 4 cut(s) 112, 330, 347, 418
HphI GGTGA 2 cut(s) 193, 407
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 6 cut(s) 102, 236, 468, 538, 561, 584
Hpy188III TCNNGA 7 cut(s) 83, 247, 314, 437, 448, 548, 635
Hpy8I GTNNAC 1 cut(s) 117
HpyAV CCTTC 1 cut(s) 262
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4V TGCA 3 cut(s) 221, 280, 290
HpyF3I CTNAG 6 cut(s) 233, 438, 446, 467, 581, 588
Hsp92II CATG 2 cut(s) 76, 268
KspAI GTTAAC 1 cut(s) 117
Kzo9I GATC 5 cut(s) 166, 366, 544, 556, 601
LguI GCTCTTC 1 cut(s) 519
Lsp1109I GCAGC 2 cut(s) 233, 355
LweI GCATC 1 cut(s) 208
MaeI CTAG 5 cut(s) 105, 132, 149, 486, 495
MaeIII GTNAC 1 cut(s) 301
MalI GATC 5 cut(s) 168, 368, 546, 558, 603
MboI GATC 5 cut(s) 166, 366, 544, 556, 601
MboII GAAGA 9 cut(s) 31, 284, 393, 455, 483, 536, 551, 554, 623
MflI RGATCY 2 cut(s) 366, 544
MluCI AATT 2 cut(s) 323, 622
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 6 cut(s) 15, 292, 321, 410, 455, 585
MseI TTAA 1 cut(s) 116
MspI CCGG 4 cut(s) 112, 330, 347, 418
NdeI CATATG 1 cut(s) 576
NdeII GATC 5 cut(s) 166, 366, 544, 556, 601
NlaIII CATG 2 cut(s) 76, 268
NlaIV GGNNCC 1 cut(s) 87
PciSI GCTCTTC 1 cut(s) 519
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 2 cut(s) 223, 345
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 1 cut(s) 332
PsuI RGATCY 2 cut(s) 366, 544
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 2 cut(s) 222, 344
Sau3AI GATC 5 cut(s) 166, 366, 544, 556, 601
Sau96I GGNCC 1 cut(s) 332
SetI ASST 9 cut(s) 133, 212, 226, 303, 447, 479, 496, 556, 589
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 1 cut(s) 478
SinI GGWCC 1 cut(s) 332
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 2 cut(s) 323, 622
SspMI CTAG 5 cut(s) 105, 132, 149, 486, 495
TaaI ACNGT 1 cut(s) 230
TaqI TCGA 1 cut(s) 408
TasI AATT 2 cut(s) 323, 622
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 2 cut(s) 233, 508
TseI GCWGC 2 cut(s) 221, 343
TspDTI ATGAA 2 cut(s) 281, 624
TspGWI ACGGA 1 cut(s) 266
TspRI CASTG 2 cut(s) 233, 508
Van91I CCANNNNNTGG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 1 cut(s) 622
XspI CTAG 5 cut(s) 105, 132, 149, 486, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.