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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 279 |
| AcsI | RAATTY | 3 cut(s) | 100, 254, 373 |
| AcuI | CTGAAG | 1 cut(s) | 257 |
| AfaI | GTAC | 3 cut(s) | 66, 402, 408 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 229 |
| AgsI | TTSAA | 3 cut(s) | 260, 331, 378 |
| AluBI | AGCT | 2 cut(s) | 18, 182 |
| AluI | AGCT | 2 cut(s) | 18, 182 |
| Alw21I | GWGCWC | 1 cut(s) | 20 |
| Alw26I | GTCTC | 1 cut(s) | 329 |
| ApoI | RAATTY | 3 cut(s) | 100, 254, 373 |
| AsuHPI | GGTGA | 1 cut(s) | 289 |
| BanII | GRGCYC | 1 cut(s) | 20 |
| Bbv12I | GWGCWC | 1 cut(s) | 20 |
| BccI | CCATC | 1 cut(s) | 223 |
| BcoDI | GTCTC | 1 cut(s) | 329 |
| BfaI | CTAG | 4 cut(s) | 173, 185, 342, 359 |
| BglII | AGATCT | 1 cut(s) | 425 |
| BsaJI | CCNNGG | 1 cut(s) | 247 |
| BsaWI | WCCGGW | 1 cut(s) | 299 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 229 |
| Bse1I | ACTGG | 1 cut(s) | 359 |
| BseDI | CCNNGG | 1 cut(s) | 247 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 229 |
| BseMII | CTCAG | 1 cut(s) | 429 |
| BseNI | ACTGG | 1 cut(s) | 359 |
| BsiHKAI | GWGCWC | 1 cut(s) | 20 |
| BsiSI | CCGG | 1 cut(s) | 300 |
| BslFI | GGGAC | 1 cut(s) | 101 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 229 |
| BsmAI | GTCTC | 1 cut(s) | 329 |
| BsmFI | GGGAC | 1 cut(s) | 101 |
| Bsp1286I | GDGCHC | 1 cut(s) | 20 |
| Bsp1407I | TGTACA | 1 cut(s) | 64 |
| Bsp143I | GATC | 2 cut(s) | 410, 425 |
| BspCNI | CTCAG | 1 cut(s) | 430 |
| BsrGI | TGTACA | 1 cut(s) | 64 |
| BsrI | ACTGG | 1 cut(s) | 359 |
| BssECI | CCNNGG | 1 cut(s) | 247 |
| BssMI | GATC | 2 cut(s) | 410, 425 |
| BssNAI | GTATAC | 1 cut(s) | 280 |
| BssT1I | CCWWGG | 1 cut(s) | 247 |
| Bst1107I | GTATAC | 1 cut(s) | 280 |
| BstAUI | TGTACA | 1 cut(s) | 64 |
| BstDEI | CTNAG | 1 cut(s) | 438 |
| BstKTI | GATC | 2 cut(s) | 413, 428 |
| BstMAI | GTCTC | 1 cut(s) | 329 |
| BstMBI | GATC | 2 cut(s) | 410, 425 |
| BstX2I | RGATCY | 1 cut(s) | 425 |
| BstYI | RGATCY | 1 cut(s) | 425 |
| BstZ17I | GTATAC | 1 cut(s) | 280 |
| Csp6I | GTAC | 3 cut(s) | 65, 401, 407 |
| CviAII | CATG | 1 cut(s) | 155 |
| CviJI | RGCY | 7 cut(s) | 18, 74, 166, 182, 222, 233, 358 |
| CviKI_1 | RGCY | 7 cut(s) | 18, 74, 166, 182, 222, 233, 358 |
| CviQI | GTAC | 3 cut(s) | 65, 401, 407 |
| DdeI | CTNAG | 1 cut(s) | 438 |
| DpnI | GATC | 2 cut(s) | 412, 427 |
| DpnII | GATC | 2 cut(s) | 410, 425 |
| Ecl136II | GAGCTC | 1 cut(s) | 18 |
| Eco130I | CCWWGG | 1 cut(s) | 247 |
| Eco24I | GRGCYC | 1 cut(s) | 20 |
| Eco53kI | GAGCTC | 1 cut(s) | 18 |
| Eco57I | CTGAAG | 1 cut(s) | 257 |
| EcoICRI | GAGCTC | 1 cut(s) | 18 |
| EcoT14I | CCWWGG | 1 cut(s) | 247 |
| EcoT38I | GRGCYC | 1 cut(s) | 20 |
| ErhI | CCWWGG | 1 cut(s) | 247 |
| FaeI | CATG | 1 cut(s) | 158 |
| FaiI | YATR | 6 cut(s) | 35, 131, 156, 280, 285, 390 |
| FaqI | GGGAC | 1 cut(s) | 101 |
| FatI | CATG | 1 cut(s) | 154 |
| FblI | GTMKAC | 1 cut(s) | 279 |
| FriOI | GRGCYC | 1 cut(s) | 20 |
| FspBI | CTAG | 4 cut(s) | 173, 185, 342, 359 |
| HapII | CCGG | 1 cut(s) | 300 |
| Hin1II | CATG | 1 cut(s) | 158 |
| HpaII | CCGG | 1 cut(s) | 300 |
| HphI | GGTGA | 1 cut(s) | 289 |
| Hpy166II | GTNNAC | 1 cut(s) | 280 |
| Hpy188I | TCNGA | 1 cut(s) | 439 |
| Hpy188III | TCNNGA | 3 cut(s) | 173, 185, 203 |
| Hpy8I | GTNNAC | 1 cut(s) | 280 |
| HpyAV | CCTTC | 1 cut(s) | 281 |
| HpyF3I | CTNAG | 1 cut(s) | 438 |
| Hsp92II | CATG | 1 cut(s) | 158 |
| Kzo9I | GATC | 2 cut(s) | 410, 425 |
| LmnI | GCTCC | 1 cut(s) | 23 |
| LpnPI | CCDG | 9 cut(s) | 75, 132, 148, 148, 236, 260, 313, 340, 417 |
| MaeI | CTAG | 4 cut(s) | 173, 185, 342, 359 |
| MalI | GATC | 2 cut(s) | 412, 427 |
| MboI | GATC | 2 cut(s) | 410, 425 |
| MboII | GAAGA | 2 cut(s) | 17, 374 |
| MflI | RGATCY | 1 cut(s) | 425 |
| MhlI | GDGCHC | 1 cut(s) | 20 |
| MluCI | AATT | 4 cut(s) | 100, 144, 254, 373 |
| MnlI | CCTC | 2 cut(s) | 115, 338 |
| MspI | CCGG | 1 cut(s) | 300 |
| NdeII | GATC | 2 cut(s) | 410, 425 |
| NlaIII | CATG | 1 cut(s) | 158 |
| Psp124BI | GAGCTC | 1 cut(s) | 20 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 399, 431 |
| PsuI | RGATCY | 1 cut(s) | 425 |
| RsaI | GTAC | 3 cut(s) | 66, 402, 408 |
| RsaNI | GTAC | 3 cut(s) | 65, 401, 407 |
| SacI | GAGCTC | 1 cut(s) | 20 |
| Sau3AI | GATC | 2 cut(s) | 410, 425 |
| SduI | GDGCHC | 1 cut(s) | 20 |
| SetI | ASST | 5 cut(s) | 20, 184, 273, 279, 406 |
| Sse9I | AATT | 4 cut(s) | 100, 144, 254, 373 |
| SspMI | CTAG | 4 cut(s) | 173, 185, 342, 359 |
| SstI | GAGCTC | 1 cut(s) | 20 |
| StyI | CCWWGG | 1 cut(s) | 247 |
| TasI | AATT | 4 cut(s) | 100, 144, 254, 373 |
| TatI | WGTACW | 1 cut(s) | 64 |
| TspDTI | ATGAA | 3 cut(s) | 19, 138, 300 |
| TspGWI | ACGGA | 1 cut(s) | 375 |
| XapI | RAATTY | 3 cut(s) | 100, 254, 373 |
| XbaI | TCTAGA | 2 cut(s) | 172, 184 |
| XmiI | GTMKAC | 1 cut(s) | 279 |
| XspI | CTAG | 4 cut(s) | 173, 185, 342, 359 |