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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 50 |
| AcsI | RAATTY | 5 cut(s) | 95, 200, 244, 303, 398 |
| AcuI | CTGAAG | 1 cut(s) | 401 |
| AfaI | GTAC | 2 cut(s) | 285, 425 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 373 |
| AgsI | TTSAA | 4 cut(s) | 143, 404, 431, 475 |
| AjnI | CCWGG | 1 cut(s) | 262 |
| AluBI | AGCT | 5 cut(s) | 41, 162, 196, 326, 407 |
| AluI | AGCT | 5 cut(s) | 41, 162, 196, 326, 407 |
| Alw26I | GTCTC | 1 cut(s) | 473 |
| AlwI | GGATC | 1 cut(s) | 50 |
| AoxI | GGCC | 1 cut(s) | 216 |
| ApoI | RAATTY | 5 cut(s) | 95, 200, 244, 303, 398 |
| AspS9I | GGNCC | 1 cut(s) | 217 |
| AsuC2I | CCSGG | 1 cut(s) | 445 |
| AsuHPI | GGTGA | 1 cut(s) | 433 |
| BccI | CCATC | 2 cut(s) | 182, 367 |
| BciT130I | CCWGG | 1 cut(s) | 264 |
| BcnI | CCSGG | 1 cut(s) | 445 |
| BcoDI | GTCTC | 1 cut(s) | 473 |
| BfaI | CTAG | 3 cut(s) | 279, 311, 329 |
| BglII | AGATCT | 1 cut(s) | 313 |
| Bme1390I | CCNGG | 2 cut(s) | 264, 445 |
| BmgT120I | GGNCC | 1 cut(s) | 217 |
| BmiI | GGNNCC | 1 cut(s) | 219 |
| BmrFI | CCNGG | 2 cut(s) | 264, 445 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 328, 360 |
| BpmI | CTGGAG | 1 cut(s) | 148 |
| BpuMI | CCSGG | 1 cut(s) | 445 |
| BsaJI | CCNNGG | 4 cut(s) | 262, 263, 391, 444 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 373 |
| BseBI | CCWGG | 1 cut(s) | 264 |
| BseDI | CCNNGG | 4 cut(s) | 262, 263, 391, 444 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 373 |
| BseMII | CTCAG | 1 cut(s) | 33 |
| BshFI | GGCC | 1 cut(s) | 218 |
| BsiSI | CCGG | 1 cut(s) | 444 |
| BslFI | GGGAC | 2 cut(s) | 245, 281 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 373 |
| BsmAI | GTCTC | 1 cut(s) | 473 |
| BsmFI | GGGAC | 2 cut(s) | 245, 281 |
| BsnI | GGCC | 1 cut(s) | 218 |
| Bsp143I | GATC | 4 cut(s) | 25, 55, 313, 412 |
| BspANI | GGCC | 1 cut(s) | 218 |
| BspCNI | CTCAG | 1 cut(s) | 34 |
| BspLI | GGNNCC | 1 cut(s) | 219 |
| BspPI | GGATC | 1 cut(s) | 50 |
| BssECI | CCNNGG | 4 cut(s) | 262, 263, 391, 444 |
| BssMI | GATC | 4 cut(s) | 25, 55, 313, 412 |
| BssT1I | CCWWGG | 1 cut(s) | 391 |
| Bst2UI | CCWGG | 1 cut(s) | 264 |
| BstDEI | CTNAG | 1 cut(s) | 42 |
| BstKTI | GATC | 4 cut(s) | 28, 58, 316, 415 |
| BstMAI | GTCTC | 1 cut(s) | 473 |
| BstMBI | GATC | 4 cut(s) | 25, 55, 313, 412 |
| BstNI | CCWGG | 1 cut(s) | 264 |
| BstSCI | CCNGG | 2 cut(s) | 262, 443 |
| BstX2I | RGATCY | 2 cut(s) | 55, 313 |
| BstYI | RGATCY | 2 cut(s) | 55, 313 |
| BsuRI | GGCC | 1 cut(s) | 218 |
| Cfr13I | GGNCC | 1 cut(s) | 217 |
| Csp6I | GTAC | 2 cut(s) | 284, 424 |
| CviQI | GTAC | 2 cut(s) | 284, 424 |
| DdeI | CTNAG | 1 cut(s) | 42 |
| DpnI | GATC | 4 cut(s) | 27, 57, 315, 414 |
| DpnII | GATC | 4 cut(s) | 25, 55, 313, 412 |
| DraI | TTTAAA | 1 cut(s) | 100 |
| Eco130I | CCWWGG | 1 cut(s) | 391 |
| Eco57I | CTGAAG | 1 cut(s) | 401 |
| EcoO109I | RGGNCCY | 1 cut(s) | 217 |
| EcoRII | CCWGG | 1 cut(s) | 262 |
| EcoT14I | CCWWGG | 1 cut(s) | 391 |
| ErhI | CCWWGG | 1 cut(s) | 391 |
| FaiI | YATR | 4 cut(s) | 24, 33, 130, 209 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 449, 481 |
| FaqI | GGGAC | 2 cut(s) | 245, 281 |
| FspBI | CTAG | 3 cut(s) | 279, 311, 329 |
| GsuI | CTGGAG | 1 cut(s) | 148 |
| HaeIII | GGCC | 1 cut(s) | 218 |
| HapII | CCGG | 1 cut(s) | 444 |
| HindIII | AAGCTT | 1 cut(s) | 194 |
| HpaII | CCGG | 1 cut(s) | 444 |
| HphI | GGTGA | 1 cut(s) | 433 |
| Hpy188I | TCNGA | 2 cut(s) | 107, 420 |
| Hpy188III | TCNNGA | 8 cut(s) | 59, 134, 165, 182, 317, 329, 347, 410 |
| HpyF3I | CTNAG | 1 cut(s) | 42 |
| Kzo9I | GATC | 4 cut(s) | 25, 55, 313, 412 |
| LmnI | GCTCC | 2 cut(s) | 167, 412 |
| LpnPI | CCDG | 8 cut(s) | 72, 178, 249, 276, 302, 380, 423, 457 |
| MaeI | CTAG | 3 cut(s) | 279, 311, 329 |
| MalI | GATC | 4 cut(s) | 27, 57, 315, 414 |
| MboI | GATC | 4 cut(s) | 25, 55, 313, 412 |
| MboII | GAAGA | 5 cut(s) | 47, 65, 95, 161, 407 |
| MflI | RGATCY | 2 cut(s) | 55, 313 |
| MluCI | AATT | 7 cut(s) | 46, 95, 200, 244, 288, 303, 398 |
| MmeI | TCCRAC | 1 cut(s) | 318 |
| MnlI | CCTC | 2 cut(s) | 9, 482 |
| MseI | TTAA | 3 cut(s) | 6, 99, 198 |
| MspI | CCGG | 1 cut(s) | 444 |
| MspR9I | CCNGG | 2 cut(s) | 264, 445 |
| MvaI | CCWGG | 1 cut(s) | 264 |
| NciI | CCSGG | 1 cut(s) | 445 |
| NdeII | GATC | 4 cut(s) | 25, 55, 313, 412 |
| NlaIV | GGNNCC | 1 cut(s) | 219 |
| PasI | CCCWGGG | 1 cut(s) | 263 |
| Psp6I | CCWGG | 1 cut(s) | 262 |
| PspGI | CCWGG | 1 cut(s) | 262 |
| PspN4I | GGNNCC | 1 cut(s) | 219 |
| PspPI | GGNCC | 1 cut(s) | 217 |
| PsuI | RGATCY | 2 cut(s) | 55, 313 |
| RsaI | GTAC | 2 cut(s) | 285, 425 |
| RsaNI | GTAC | 2 cut(s) | 284, 424 |
| SaqAI | TTAA | 3 cut(s) | 6, 99, 198 |
| Sau3AI | GATC | 4 cut(s) | 25, 55, 313, 412 |
| Sau96I | GGNCC | 1 cut(s) | 217 |
| ScrFI | CCNGG | 2 cut(s) | 264, 445 |
| SetI | ASST | 6 cut(s) | 43, 164, 198, 280, 328, 409 |
| Sse9I | AATT | 7 cut(s) | 46, 95, 200, 244, 288, 303, 398 |
| SspMI | CTAG | 3 cut(s) | 279, 311, 329 |
| StyD4I | CCNGG | 2 cut(s) | 262, 443 |
| StyI | CCWWGG | 1 cut(s) | 391 |
| TaqI | TCGA | 2 cut(s) | 81, 181 |
| TasI | AATT | 7 cut(s) | 46, 95, 200, 244, 288, 303, 398 |
| TatI | WGTACW | 1 cut(s) | 423 |
| Tru1I | TTAA | 3 cut(s) | 6, 99, 198 |
| Tru9I | TTAA | 3 cut(s) | 6, 99, 198 |
| TspDTI | ATGAA | 2 cut(s) | 48, 104 |
| XapI | RAATTY | 5 cut(s) | 95, 200, 244, 303, 398 |
| XbaI | TCTAGA | 1 cut(s) | 328 |
| XspI | CTAG | 3 cut(s) | 279, 311, 329 |