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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 50 |
| AcsI | RAATTY | 4 cut(s) | 93, 244, 398, 518 |
| AcuI | CTGAAG | 1 cut(s) | 401 |
| AfaI | GTAC | 2 cut(s) | 285, 425 |
| AgsI | TTSAA | 3 cut(s) | 100, 143, 523 |
| AjnI | CCWGG | 1 cut(s) | 103 |
| AluBI | AGCT | 3 cut(s) | 162, 326, 366 |
| AluI | AGCT | 3 cut(s) | 162, 326, 366 |
| Alw26I | GTCTC | 1 cut(s) | 473 |
| AlwI | GGATC | 1 cut(s) | 50 |
| ApoI | RAATTY | 4 cut(s) | 93, 244, 398, 518 |
| BccI | CCATC | 1 cut(s) | 367 |
| BciT130I | CCWGG | 1 cut(s) | 105 |
| BcoDI | GTCTC | 1 cut(s) | 473 |
| BfaI | CTAG | 4 cut(s) | 180, 317, 329, 503 |
| BglII | AGATCT | 1 cut(s) | 412 |
| Bme1390I | CCNGG | 1 cut(s) | 105 |
| BmrFI | CCNGG | 1 cut(s) | 105 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 328, 360 |
| BpmI | CTGGAG | 2 cut(s) | 255, 465 |
| BsaJI | CCNNGG | 1 cut(s) | 391 |
| Bse1I | ACTGG | 2 cut(s) | 238, 448 |
| BseBI | CCWGG | 1 cut(s) | 105 |
| BseDI | CCNNGG | 1 cut(s) | 391 |
| BseNI | ACTGG | 2 cut(s) | 238, 448 |
| BslFI | GGGAC | 1 cut(s) | 245 |
| BsmAI | GTCTC | 1 cut(s) | 473 |
| BsmFI | GGGAC | 1 cut(s) | 245 |
| Bsp143I | GATC | 4 cut(s) | 8, 25, 55, 412 |
| BspPI | GGATC | 1 cut(s) | 50 |
| BsrI | ACTGG | 2 cut(s) | 238, 448 |
| BssECI | CCNNGG | 1 cut(s) | 391 |
| BssMI | GATC | 4 cut(s) | 8, 25, 55, 412 |
| BssT1I | CCWWGG | 1 cut(s) | 391 |
| Bst2UI | CCWGG | 1 cut(s) | 105 |
| Bst6I | CTCTTC | 1 cut(s) | 501 |
| BstDEI | CTNAG | 1 cut(s) | 5 |
| BstKTI | GATC | 4 cut(s) | 11, 28, 58, 415 |
| BstMAI | GTCTC | 1 cut(s) | 473 |
| BstMBI | GATC | 4 cut(s) | 8, 25, 55, 412 |
| BstNI | CCWGG | 1 cut(s) | 105 |
| BstSCI | CCNGG | 1 cut(s) | 103 |
| BstX2I | RGATCY | 2 cut(s) | 55, 412 |
| BstYI | RGATCY | 2 cut(s) | 55, 412 |
| Csp6I | GTAC | 2 cut(s) | 284, 424 |
| CviAII | CATG | 1 cut(s) | 299 |
| CviJI | RGCY | 7 cut(s) | 162, 218, 310, 326, 366, 377, 502 |
| CviKI_1 | RGCY | 7 cut(s) | 162, 218, 310, 326, 366, 377, 502 |
| CviQI | GTAC | 2 cut(s) | 284, 424 |
| DdeI | CTNAG | 1 cut(s) | 5 |
| DpnI | GATC | 4 cut(s) | 10, 27, 57, 414 |
| DpnII | GATC | 4 cut(s) | 8, 25, 55, 412 |
| DraI | TTTAAA | 2 cut(s) | 403, 475 |
| Eam1104I | CTCTTC | 1 cut(s) | 501 |
| EarI | CTCTTC | 1 cut(s) | 501 |
| Eco130I | CCWWGG | 1 cut(s) | 391 |
| Eco57I | CTGAAG | 1 cut(s) | 401 |
| EcoRII | CCWGG | 1 cut(s) | 103 |
| EcoT14I | CCWWGG | 1 cut(s) | 391 |
| ErhI | CCWWGG | 1 cut(s) | 391 |
| FaeI | CATG | 1 cut(s) | 302 |
| FaiI | YATR | 6 cut(s) | 24, 33, 275, 300, 429, 535 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 449, 481 |
| FaqI | GGGAC | 1 cut(s) | 245 |
| FatI | CATG | 1 cut(s) | 298 |
| FspBI | CTAG | 4 cut(s) | 180, 317, 329, 503 |
| GsuI | CTGGAG | 2 cut(s) | 255, 465 |
| Hin1II | CATG | 1 cut(s) | 302 |
| HincII | GTYRAC | 1 cut(s) | 361 |
| HindII | GTYRAC | 1 cut(s) | 361 |
| HinfI | GANTC | 1 cut(s) | 44 |
| Hpy166II | GTNNAC | 2 cut(s) | 361, 424 |
| Hpy188III | TCNNGA | 5 cut(s) | 59, 134, 317, 329, 347 |
| Hpy8I | GTNNAC | 2 cut(s) | 361, 424 |
| HpyAV | CCTTC | 1 cut(s) | 146 |
| HpyF3I | CTNAG | 1 cut(s) | 5 |
| Hsp92II | CATG | 1 cut(s) | 302 |
| Kzo9I | GATC | 4 cut(s) | 8, 25, 55, 412 |
| LmnI | GCTCC | 1 cut(s) | 167 |
| MaeI | CTAG | 4 cut(s) | 180, 317, 329, 503 |
| MalI | GATC | 4 cut(s) | 10, 27, 57, 414 |
| MboI | GATC | 4 cut(s) | 8, 25, 55, 412 |
| MboII | GAAGA | 6 cut(s) | 65, 95, 116, 161, 407, 518 |
| MflI | RGATCY | 2 cut(s) | 55, 412 |
| MluCI | AATT | 5 cut(s) | 93, 244, 288, 398, 518 |
| MnlI | CCTC | 2 cut(s) | 259, 482 |
| MseI | TTAA | 2 cut(s) | 402, 474 |
| MspR9I | CCNGG | 1 cut(s) | 105 |
| MvaI | CCWGG | 1 cut(s) | 105 |
| NdeII | GATC | 4 cut(s) | 8, 25, 55, 412 |
| NlaIII | CATG | 1 cut(s) | 302 |
| PfeI | GAWTC | 1 cut(s) | 44 |
| Psp6I | CCWGG | 1 cut(s) | 103 |
| PspGI | CCWGG | 1 cut(s) | 103 |
| PsuI | RGATCY | 2 cut(s) | 55, 412 |
| RsaI | GTAC | 2 cut(s) | 285, 425 |
| RsaNI | GTAC | 2 cut(s) | 284, 424 |
| SaqAI | TTAA | 2 cut(s) | 402, 474 |
| Sau3AI | GATC | 4 cut(s) | 8, 25, 55, 412 |
| ScrFI | CCNGG | 1 cut(s) | 105 |
| SetI | ASST | 4 cut(s) | 164, 328, 368, 423 |
| Sse9I | AATT | 5 cut(s) | 93, 244, 288, 398, 518 |
| SspMI | CTAG | 4 cut(s) | 180, 317, 329, 503 |
| StyD4I | CCNGG | 1 cut(s) | 103 |
| StyI | CCWWGG | 1 cut(s) | 391 |
| TaqI | TCGA | 1 cut(s) | 81 |
| TasI | AATT | 5 cut(s) | 93, 244, 288, 398, 518 |
| TatI | WGTACW | 1 cut(s) | 423 |
| TfiI | GAWTC | 1 cut(s) | 44 |
| Tru1I | TTAA | 2 cut(s) | 402, 474 |
| Tru9I | TTAA | 2 cut(s) | 402, 474 |
| TspDTI | ATGAA | 4 cut(s) | 51, 104, 163, 444 |
| TspGWI | ACGGA | 1 cut(s) | 520 |
| XapI | RAATTY | 4 cut(s) | 93, 244, 398, 518 |
| XbaI | TCTAGA | 2 cut(s) | 316, 328 |
| XspI | CTAG | 4 cut(s) | 180, 317, 329, 503 |