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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 33 |
| AarI | CACCTGC | 1 cut(s) | 409 |
| Acc36I | ACCTGC | 1 cut(s) | 409 |
| AclWI | GGATC | 1 cut(s) | 20 |
| AcsI | RAATTY | 4 cut(s) | 95, 101, 244, 398 |
| AfaI | GTAC | 2 cut(s) | 425, 461 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 373 |
| AgsI | TTSAA | 4 cut(s) | 100, 143, 404, 475 |
| AluBI | AGCT | 4 cut(s) | 11, 19, 41, 162 |
| AluI | AGCT | 4 cut(s) | 11, 19, 41, 162 |
| Alw26I | GTCTC | 1 cut(s) | 487 |
| AlwI | GGATC | 1 cut(s) | 20 |
| ApoI | RAATTY | 4 cut(s) | 95, 101, 244, 398 |
| AseI | ATTAAT | 1 cut(s) | 77 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 95 |
| BbsI | GAAGAC | 1 cut(s) | 273 |
| BccI | CCATC | 2 cut(s) | 146, 367 |
| BcoDI | GTCTC | 1 cut(s) | 487 |
| BfaI | CTAG | 2 cut(s) | 317, 329 |
| BfuAI | ACCTGC | 1 cut(s) | 409 |
| BmcAI | AGTACT | 1 cut(s) | 461 |
| BmsI | GCATC | 1 cut(s) | 312 |
| BoxI | GACNNNNGTC | 1 cut(s) | 324 |
| BpiI | GAAGAC | 1 cut(s) | 273 |
| BpmI | CTGGAG | 2 cut(s) | 148, 465 |
| BsaJI | CCNNGG | 2 cut(s) | 222, 391 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 373 |
| Bse1I | ACTGG | 2 cut(s) | 448, 501 |
| BseDI | CCNNGG | 2 cut(s) | 222, 391 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 373 |
| BseMII | CTCAG | 2 cut(s) | 19, 33 |
| BseNI | ACTGG | 2 cut(s) | 448, 501 |
| BsgI | GTGCAG | 1 cut(s) | 366 |
| BslFI | GGGAC | 2 cut(s) | 245, 269 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 373 |
| BsmAI | GTCTC | 1 cut(s) | 487 |
| BsmFI | GGGAC | 2 cut(s) | 245, 269 |
| Bsp143I | GATC | 1 cut(s) | 25 |
| BspCNI | CTCAG | 3 cut(s) | 4, 18, 34 |
| BspMI | ACCTGC | 1 cut(s) | 409 |
| BspPI | GGATC | 1 cut(s) | 20 |
| BsrI | ACTGG | 2 cut(s) | 448, 501 |
| BssECI | CCNNGG | 2 cut(s) | 222, 391 |
| BssMI | GATC | 1 cut(s) | 25 |
| BssT1I | CCWWGG | 1 cut(s) | 391 |
| Bst4CI | ACNGT | 1 cut(s) | 172 |
| Bst6I | CTCTTC | 1 cut(s) | 485 |
| BstDEI | CTNAG | 3 cut(s) | 5, 12, 42 |
| BstDSI | CCRYGG | 1 cut(s) | 222 |
| BstKTI | GATC | 1 cut(s) | 28 |
| BstMAI | GTCTC | 1 cut(s) | 487 |
| BstMBI | GATC | 1 cut(s) | 25 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 324 |
| BstV2I | GAAGAC | 1 cut(s) | 273 |
| BtgI | CCRYGG | 1 cut(s) | 222 |
| BveI | ACCTGC | 1 cut(s) | 409 |
| Csp6I | GTAC | 2 cut(s) | 424, 460 |
| CviJI | RGCY | 8 cut(s) | 11, 19, 41, 162, 218, 366, 377, 500 |
| CviKI_1 | RGCY | 8 cut(s) | 11, 19, 41, 162, 218, 366, 377, 500 |
| CviQI | GTAC | 2 cut(s) | 424, 460 |
| DdeI | CTNAG | 3 cut(s) | 5, 12, 42 |
| DpnI | GATC | 1 cut(s) | 27 |
| DpnII | GATC | 1 cut(s) | 25 |
| Eam1104I | CTCTTC | 1 cut(s) | 485 |
| EarI | CTCTTC | 1 cut(s) | 485 |
| Eco130I | CCWWGG | 1 cut(s) | 391 |
| EcoRI | GAATTC | 1 cut(s) | 95 |
| EcoT14I | CCWWGG | 1 cut(s) | 391 |
| ErhI | CCWWGG | 1 cut(s) | 391 |
| FaiI | YATR | 6 cut(s) | 24, 33, 132, 176, 300, 429 |
| FaqI | GGGAC | 2 cut(s) | 245, 269 |
| FspBI | CTAG | 2 cut(s) | 317, 329 |
| GsuI | CTGGAG | 2 cut(s) | 148, 465 |
| HinfI | GANTC | 2 cut(s) | 282, 325 |
| Hpy166II | GTNNAC | 1 cut(s) | 424 |
| Hpy188I | TCNGA | 3 cut(s) | 8, 107, 213 |
| Hpy188III | TCNNGA | 4 cut(s) | 59, 165, 317, 329 |
| Hpy8I | GTNNAC | 1 cut(s) | 424 |
| HpyCH4III | ACNGT | 1 cut(s) | 172 |
| HpyCH4V | TGCA | 2 cut(s) | 383, 418 |
| HpyF3I | CTNAG | 3 cut(s) | 5, 12, 42 |
| Kzo9I | GATC | 1 cut(s) | 25 |
| LmnI | GCTCC | 1 cut(s) | 167 |
| LweI | GCATC | 1 cut(s) | 312 |
| MaeI | CTAG | 2 cut(s) | 317, 329 |
| MalI | GATC | 1 cut(s) | 27 |
| MboI | GATC | 1 cut(s) | 25 |
| MboII | GAAGA | 5 cut(s) | 95, 146, 161, 278, 502 |
| MluCI | AATT | 6 cut(s) | 46, 74, 95, 101, 244, 398 |
| MlyI | GAGTC | 2 cut(s) | 291, 334 |
| MnlI | CCTC | 1 cut(s) | 480 |
| MroXI | GAANNNNTTC | 1 cut(s) | 95 |
| MseI | TTAA | 2 cut(s) | 77, 311 |
| NdeII | GATC | 1 cut(s) | 25 |
| PaqCI | CACCTGC | 1 cut(s) | 409 |
| PdmI | GAANNNNTTC | 1 cut(s) | 95 |
| PleI | GAGTC | 2 cut(s) | 290, 333 |
| PpsI | GAGTC | 2 cut(s) | 290, 333 |
| PshAI | GACNNNNGTC | 1 cut(s) | 324 |
| PshBI | ATTAAT | 1 cut(s) | 77 |
| PsiI | TTATAA | 1 cut(s) | 33 |
| RsaI | GTAC | 2 cut(s) | 425, 461 |
| RsaNI | GTAC | 2 cut(s) | 424, 460 |
| SaqAI | TTAA | 2 cut(s) | 77, 311 |
| Sau3AI | GATC | 1 cut(s) | 25 |
| ScaI | AGTACT | 1 cut(s) | 461 |
| SchI | GAGTC | 2 cut(s) | 291, 334 |
| SetI | ASST | 6 cut(s) | 13, 21, 43, 164, 423, 468 |
| SfaNI | GCATC | 1 cut(s) | 312 |
| Sse9I | AATT | 6 cut(s) | 46, 74, 95, 101, 244, 398 |
| SspMI | CTAG | 2 cut(s) | 317, 329 |
| StyI | CCWWGG | 1 cut(s) | 391 |
| TaaI | ACNGT | 1 cut(s) | 172 |
| TaqI | TCGA | 1 cut(s) | 81 |
| TasI | AATT | 6 cut(s) | 46, 74, 95, 101, 244, 398 |
| TatI | WGTACW | 2 cut(s) | 423, 459 |
| Tru1I | TTAA | 2 cut(s) | 77, 311 |
| Tru9I | TTAA | 2 cut(s) | 77, 311 |
| TspDTI | ATGAA | 3 cut(s) | 104, 147, 444 |
| VspI | ATTAAT | 1 cut(s) | 77 |
| XapI | RAATTY | 4 cut(s) | 95, 101, 244, 398 |
| XbaI | TCTAGA | 2 cut(s) | 316, 328 |
| XmnI | GAANNNNTTC | 1 cut(s) | 95 |
| XspI | CTAG | 2 cut(s) | 317, 329 |
| ZrmI | AGTACT | 1 cut(s) | 461 |