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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 5 cut(s) | 45, 244, 398, 517, 580 |
| AcuI | CTGAAG | 1 cut(s) | 401 |
| AcyI | GRCGYC | 1 cut(s) | 377 |
| AfaI | GTAC | 4 cut(s) | 285, 425, 546, 554 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 373 |
| AgsI | TTSAA | 5 cut(s) | 100, 143, 404, 475, 522 |
| AjnI | CCWGG | 2 cut(s) | 58, 103 |
| AluBI | AGCT | 2 cut(s) | 19, 162 |
| AluI | AGCT | 2 cut(s) | 19, 162 |
| Alw26I | GTCTC | 1 cut(s) | 473 |
| ApoI | RAATTY | 5 cut(s) | 45, 244, 398, 517, 580 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 95 |
| AsuHPI | GGTGA | 1 cut(s) | 169 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 258, 290 |
| BbsI | GAAGAC | 1 cut(s) | 273 |
| BccI | CCATC | 1 cut(s) | 367 |
| BciT130I | CCWGG | 2 cut(s) | 60, 105 |
| BcoDI | GTCTC | 1 cut(s) | 473 |
| BfaI | CTAG | 2 cut(s) | 317, 329 |
| BglII | AGATCT | 1 cut(s) | 571 |
| Bme1390I | CCNGG | 2 cut(s) | 60, 105 |
| BmrFI | CCNGG | 2 cut(s) | 60, 105 |
| BpiI | GAAGAC | 1 cut(s) | 273 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 328, 360 |
| BpmI | CTGGAG | 1 cut(s) | 465 |
| BsaHI | GRCGYC | 1 cut(s) | 377 |
| BsaJI | CCNNGG | 2 cut(s) | 59, 391 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 373 |
| Bse1I | ACTGG | 2 cut(s) | 448, 503 |
| BseBI | CCWGG | 2 cut(s) | 60, 105 |
| BseDI | CCNNGG | 2 cut(s) | 59, 391 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 373 |
| BseNI | ACTGG | 2 cut(s) | 448, 503 |
| BslFI | GGGAC | 1 cut(s) | 245 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 373 |
| BsmAI | GTCTC | 1 cut(s) | 473 |
| BsmFI | GGGAC | 1 cut(s) | 245 |
| Bsp143I | GATC | 3 cut(s) | 25, 556, 571 |
| BsrI | ACTGG | 2 cut(s) | 448, 503 |
| BssECI | CCNNGG | 2 cut(s) | 59, 391 |
| BssMI | GATC | 3 cut(s) | 25, 556, 571 |
| BssNI | GRCGYC | 1 cut(s) | 377 |
| BssT1I | CCWWGG | 1 cut(s) | 391 |
| Bst2UI | CCWGG | 2 cut(s) | 60, 105 |
| BstACI | GRCGYC | 1 cut(s) | 377 |
| BstKTI | GATC | 3 cut(s) | 28, 559, 574 |
| BstMAI | GTCTC | 1 cut(s) | 473 |
| BstMBI | GATC | 3 cut(s) | 25, 556, 571 |
| BstNI | CCWGG | 2 cut(s) | 60, 105 |
| BstSCI | CCNGG | 2 cut(s) | 58, 103 |
| BstV2I | GAAGAC | 1 cut(s) | 273 |
| BstX2I | RGATCY | 1 cut(s) | 571 |
| BstYI | RGATCY | 1 cut(s) | 571 |
| BtsIMutI | CAGTG | 1 cut(s) | 441 |
| CseI | GACGC | 1 cut(s) | 366 |
| Csp6I | GTAC | 4 cut(s) | 284, 424, 545, 553 |
| CviAII | CATG | 1 cut(s) | 299 |
| CviJI | RGCY | 7 cut(s) | 19, 162, 218, 310, 326, 366, 502 |
| CviKI_1 | RGCY | 7 cut(s) | 19, 162, 218, 310, 326, 366, 502 |
| CviQI | GTAC | 4 cut(s) | 284, 424, 545, 553 |
| DpnI | GATC | 3 cut(s) | 27, 558, 573 |
| DpnII | GATC | 3 cut(s) | 25, 556, 571 |
| Eco130I | CCWWGG | 1 cut(s) | 391 |
| Eco57I | CTGAAG | 1 cut(s) | 401 |
| EcoRII | CCWGG | 2 cut(s) | 58, 103 |
| EcoT14I | CCWWGG | 1 cut(s) | 391 |
| ErhI | CCWWGG | 1 cut(s) | 391 |
| FaeI | CATG | 1 cut(s) | 302 |
| FaiI | YATR | 6 cut(s) | 24, 33, 275, 300, 429, 534 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 186, 218, 486, 518 |
| FaqI | GGGAC | 1 cut(s) | 245 |
| FatI | CATG | 1 cut(s) | 298 |
| FspBI | CTAG | 2 cut(s) | 317, 329 |
| GsuI | CTGGAG | 1 cut(s) | 465 |
| HgaI | GACGC | 1 cut(s) | 366 |
| Hin1I | GRCGYC | 1 cut(s) | 377 |
| Hin1II | CATG | 1 cut(s) | 302 |
| HinfI | GANTC | 1 cut(s) | 313 |
| HphI | GGTGA | 1 cut(s) | 169 |
| Hpy166II | GTNNAC | 1 cut(s) | 424 |
| Hpy188I | TCNGA | 1 cut(s) | 213 |
| Hpy188III | TCNNGA | 4 cut(s) | 134, 317, 329, 347 |
| Hpy8I | GTNNAC | 1 cut(s) | 424 |
| HpyAV | CCTTC | 2 cut(s) | 134, 146 |
| HpyCH4V | TGCA | 1 cut(s) | 542 |
| Hsp92I | GRCGYC | 1 cut(s) | 377 |
| Hsp92II | CATG | 1 cut(s) | 302 |
| Kzo9I | GATC | 3 cut(s) | 25, 556, 571 |
| LmnI | GCTCC | 1 cut(s) | 167 |
| MaeI | CTAG | 2 cut(s) | 317, 329 |
| MalI | GATC | 3 cut(s) | 27, 558, 573 |
| MboI | GATC | 3 cut(s) | 25, 556, 571 |
| MboII | GAAGA | 6 cut(s) | 18, 47, 95, 161, 278, 407 |
| MflI | RGATCY | 1 cut(s) | 571 |
| MluCI | AATT | 6 cut(s) | 45, 244, 288, 398, 517, 580 |
| MnlI | CCTC | 2 cut(s) | 304, 482 |
| MroXI | GAANNNNTTC | 1 cut(s) | 95 |
| MseI | TTAA | 1 cut(s) | 504 |
| MspR9I | CCNGG | 2 cut(s) | 60, 105 |
| MvaI | CCWGG | 2 cut(s) | 60, 105 |
| NdeII | GATC | 3 cut(s) | 25, 556, 571 |
| NlaIII | CATG | 1 cut(s) | 302 |
| PdmI | GAANNNNTTC | 1 cut(s) | 95 |
| PfeI | GAWTC | 1 cut(s) | 313 |
| PfoI | TCCNGGA | 1 cut(s) | 103 |
| Psp6I | CCWGG | 2 cut(s) | 58, 103 |
| PspGI | CCWGG | 2 cut(s) | 58, 103 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 545, 577 |
| PsuI | RGATCY | 1 cut(s) | 571 |
| RsaI | GTAC | 4 cut(s) | 285, 425, 546, 554 |
| RsaNI | GTAC | 4 cut(s) | 284, 424, 545, 553 |
| SaqAI | TTAA | 1 cut(s) | 504 |
| Sau3AI | GATC | 3 cut(s) | 25, 556, 571 |
| ScrFI | CCNGG | 2 cut(s) | 60, 105 |
| SetI | ASST | 4 cut(s) | 21, 98, 164, 423 |
| Sse9I | AATT | 6 cut(s) | 45, 244, 288, 398, 517, 580 |
| SspMI | CTAG | 2 cut(s) | 317, 329 |
| StyD4I | CCNGG | 2 cut(s) | 58, 103 |
| StyI | CCWWGG | 1 cut(s) | 391 |
| TaqI | TCGA | 1 cut(s) | 81 |
| TasI | AATT | 6 cut(s) | 45, 244, 288, 398, 517, 580 |
| TatI | WGTACW | 1 cut(s) | 423 |
| TfiI | GAWTC | 1 cut(s) | 313 |
| Tru1I | TTAA | 1 cut(s) | 504 |
| Tru9I | TTAA | 1 cut(s) | 504 |
| TscAI | CASTG | 1 cut(s) | 448 |
| TspDTI | ATGAA | 4 cut(s) | 48, 104, 163, 444 |
| TspRI | CASTG | 1 cut(s) | 448 |
| XapI | RAATTY | 5 cut(s) | 45, 244, 398, 517, 580 |
| XbaI | TCTAGA | 2 cut(s) | 316, 328 |
| XmnI | GAANNNNTTC | 1 cut(s) | 95 |
| XspI | CTAG | 2 cut(s) | 317, 329 |