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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 4 cut(s) | 6, 157, 311, 430 |
| AcuI | CTGAAG | 1 cut(s) | 314 |
| AfaI | GTAC | 3 cut(s) | 198, 338, 459 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 286 |
| AgsI | TTSAA | 3 cut(s) | 13, 56, 435 |
| AjnI | CCWGG | 1 cut(s) | 16 |
| AluBI | AGCT | 2 cut(s) | 75, 239 |
| AluI | AGCT | 2 cut(s) | 75, 239 |
| Alw26I | GTCTC | 1 cut(s) | 386 |
| AoxI | GGCC | 1 cut(s) | 288 |
| ApoI | RAATTY | 4 cut(s) | 6, 157, 311, 430 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 8 |
| AsuHPI | GGTGA | 1 cut(s) | 346 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 171, 203 |
| BbsI | GAAGAC | 1 cut(s) | 186 |
| BccI | CCATC | 2 cut(s) | 95, 280 |
| BciT130I | CCWGG | 1 cut(s) | 18 |
| BclI | TGATCA | 1 cut(s) | 468 |
| BcoDI | GTCTC | 1 cut(s) | 386 |
| BfaI | CTAG | 2 cut(s) | 230, 242 |
| BglII | AGATCT | 2 cut(s) | 325, 483 |
| Bme1390I | CCNGG | 1 cut(s) | 18 |
| BmrFI | CCNGG | 1 cut(s) | 18 |
| BpiI | GAAGAC | 1 cut(s) | 186 |
| BsaJI | CCNNGG | 1 cut(s) | 304 |
| BsaWI | WCCGGW | 1 cut(s) | 356 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 286 |
| Bse1I | ACTGG | 1 cut(s) | 416 |
| BseBI | CCWGG | 1 cut(s) | 18 |
| BseDI | CCNNGG | 1 cut(s) | 304 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 286 |
| BseNI | ACTGG | 1 cut(s) | 416 |
| BshFI | GGCC | 1 cut(s) | 290 |
| BsiSI | CCGG | 1 cut(s) | 357 |
| BslFI | GGGAC | 1 cut(s) | 158 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 286 |
| BsmAI | GTCTC | 1 cut(s) | 386 |
| BsmFI | GGGAC | 1 cut(s) | 158 |
| BsnI | GGCC | 1 cut(s) | 290 |
| Bsp143I | GATC | 3 cut(s) | 325, 468, 483 |
| BspANI | GGCC | 1 cut(s) | 290 |
| BsrI | ACTGG | 1 cut(s) | 416 |
| BssECI | CCNNGG | 1 cut(s) | 304 |
| BssMI | GATC | 3 cut(s) | 325, 468, 483 |
| BssT1I | CCWWGG | 1 cut(s) | 304 |
| Bst2UI | CCWGG | 1 cut(s) | 18 |
| BstKTI | GATC | 3 cut(s) | 328, 471, 486 |
| BstMAI | GTCTC | 1 cut(s) | 386 |
| BstMBI | GATC | 3 cut(s) | 325, 468, 483 |
| BstNI | CCWGG | 1 cut(s) | 18 |
| BstSCI | CCNGG | 1 cut(s) | 16 |
| BstV2I | GAAGAC | 1 cut(s) | 186 |
| BstX2I | RGATCY | 2 cut(s) | 325, 483 |
| BstYI | RGATCY | 2 cut(s) | 325, 483 |
| BsuRI | GGCC | 1 cut(s) | 290 |
| Csp6I | GTAC | 3 cut(s) | 197, 337, 458 |
| CviAII | CATG | 1 cut(s) | 212 |
| CviJI | RGCY | 6 cut(s) | 75, 131, 239, 279, 290, 415 |
| CviKI_1 | RGCY | 6 cut(s) | 75, 131, 239, 279, 290, 415 |
| CviQI | GTAC | 3 cut(s) | 197, 337, 458 |
| DpnI | GATC | 3 cut(s) | 327, 470, 485 |
| DpnII | GATC | 3 cut(s) | 325, 468, 483 |
| DraI | TTTAAA | 2 cut(s) | 316, 388 |
| Eco130I | CCWWGG | 1 cut(s) | 304 |
| Eco57I | CTGAAG | 1 cut(s) | 314 |
| EcoRII | CCWGG | 1 cut(s) | 16 |
| EcoT14I | CCWWGG | 1 cut(s) | 304 |
| ErhI | CCWWGG | 1 cut(s) | 304 |
| FaeI | CATG | 1 cut(s) | 215 |
| FaiI | YATR | 5 cut(s) | 188, 213, 342, 447, 467 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 362, 394, 399, 431 |
| FaqI | GGGAC | 1 cut(s) | 158 |
| FatI | CATG | 1 cut(s) | 211 |
| FbaI | TGATCA | 1 cut(s) | 468 |
| FspBI | CTAG | 2 cut(s) | 230, 242 |
| HaeIII | GGCC | 1 cut(s) | 290 |
| HapII | CCGG | 1 cut(s) | 357 |
| Hin1II | CATG | 1 cut(s) | 215 |
| HpaII | CCGG | 1 cut(s) | 357 |
| HphI | GGTGA | 1 cut(s) | 346 |
| Hpy166II | GTNNAC | 1 cut(s) | 337 |
| Hpy188III | TCNNGA | 4 cut(s) | 47, 230, 242, 260 |
| Hpy8I | GTNNAC | 1 cut(s) | 337 |
| HpyAV | CCTTC | 2 cut(s) | 47, 59 |
| HpyCH4V | TGCA | 1 cut(s) | 455 |
| Hsp92II | CATG | 1 cut(s) | 215 |
| Ksp22I | TGATCA | 1 cut(s) | 468 |
| Kzo9I | GATC | 3 cut(s) | 325, 468, 483 |
| LmnI | GCTCC | 1 cut(s) | 80 |
| MaeI | CTAG | 2 cut(s) | 230, 242 |
| MalI | GATC | 3 cut(s) | 327, 470, 485 |
| MboI | GATC | 3 cut(s) | 325, 468, 483 |
| MboII | GAAGA | 3 cut(s) | 74, 191, 320 |
| MflI | RGATCY | 2 cut(s) | 325, 483 |
| MluCI | AATT | 6 cut(s) | 6, 157, 201, 311, 430, 472 |
| MnlI | CCTC | 3 cut(s) | 142, 217, 301 |
| MroXI | GAANNNNTTC | 1 cut(s) | 8 |
| MseI | TTAA | 4 cut(s) | 224, 299, 315, 387 |
| MspI | CCGG | 1 cut(s) | 357 |
| MspR9I | CCNGG | 1 cut(s) | 18 |
| MvaI | CCWGG | 1 cut(s) | 18 |
| NdeII | GATC | 3 cut(s) | 325, 468, 483 |
| NlaIII | CATG | 1 cut(s) | 215 |
| PdmI | GAANNNNTTC | 1 cut(s) | 8 |
| Psp6I | CCWGG | 1 cut(s) | 16 |
| PspGI | CCWGG | 1 cut(s) | 16 |
| PsuI | RGATCY | 2 cut(s) | 325, 483 |
| RsaI | GTAC | 3 cut(s) | 198, 338, 459 |
| RsaNI | GTAC | 3 cut(s) | 197, 337, 458 |
| SaqAI | TTAA | 4 cut(s) | 224, 299, 315, 387 |
| Sau3AI | GATC | 3 cut(s) | 325, 468, 483 |
| ScrFI | CCNGG | 1 cut(s) | 18 |
| SetI | ASST | 3 cut(s) | 77, 241, 336 |
| Sse9I | AATT | 6 cut(s) | 6, 157, 201, 311, 430, 472 |
| SspMI | CTAG | 2 cut(s) | 230, 242 |
| StyD4I | CCNGG | 1 cut(s) | 16 |
| StyI | CCWWGG | 1 cut(s) | 304 |
| TasI | AATT | 6 cut(s) | 6, 157, 201, 311, 430, 472 |
| TatI | WGTACW | 1 cut(s) | 336 |
| Tru1I | TTAA | 4 cut(s) | 224, 299, 315, 387 |
| Tru9I | TTAA | 4 cut(s) | 224, 299, 315, 387 |
| TspDTI | ATGAA | 2 cut(s) | 17, 357 |
| XapI | RAATTY | 4 cut(s) | 6, 157, 311, 430 |
| XbaI | TCTAGA | 2 cut(s) | 229, 241 |
| XmnI | GAANNNNTTC | 1 cut(s) | 8 |
| XspI | CTAG | 2 cut(s) | 230, 242 |