RLG00000019185

Mitochondrial inner membrane protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000640
Physical Location & Seq
Reverse (-)
47953526 .. 47955293
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019185

Sequence Viewer

Length: 690 bp
ATGATATTGAAAAAGAAAACCACGCGCAAGTGCCTCTTTCTGTCTTCGACCTTCAAGCCATGGCGCAATCATGGATCAGTTCCACAACTAATCGTCATCAACCATGTGTGCCTTCATCTCCGTCTTCCATCTTCCTCACCTCCTACTTCTTCGATTGCCAAGAAATCTGATCGTTGCCGGACTGCGATGTACTCCCGAATGCCAGATTCAATGTTCGCCGCCGCCGATGGATTCCTGAAGACTTCGCTTTTCTGCTGGAGCATCTGGAGAAATCGATTTTGGATCTACTCCGACGGTGAAGTTTCTGCTGGAGCATCAGTCTTAATCGAGTTTGGATCTGCTCCGATGGTGAAGTTTCTGCTGGAGCATTTGGAGAAATCTGGGTGTGGGATATTAGACGGGTCTTGCTGGGCCGTTCATTGCGAGAAGAAGATTGCCGGAGTATACACCCGCCGCGGATGGATAATGGTGTGTAGTAATCACATGAACATGCAAGATGAGGTCAACCAAGTAGTGATACATGAGCTAATTCATGCTTTTGATGATTGTCGGGCTGCTCCGATGGTGAACTGGGCTAATTGCCTTCATCATGCTTGTAGCGAGATTCGTGCTGGCCATCTTAGTGGTGATTGCCACTGTAAACGTGAATTTTTGTTGTGCCAAGAAGATTCGAGAATTGTGAACAAGTGA

Protein Analysis

230

Amino Acids

25.95

Weight (kDa)

8.86

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M76 PF09768 113 - 218 1.8e-32 Peptidase M76 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000640)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0331021 RchiOBHm_Chr1g0335951 RchiOBHm_Chr1g0338001 RchiOBHm_Chr6g0276241 RchiOBHm_Chr6g0297731 RchiOBHm_Chr7g0188211 RchiOBHm_Chr7g0217921 RchiOBHm_Chr7g0238211
rosa_laevigata RLG00000007457 RLG00000015447 RLG00000019185 RLG00000032916 RLG00000034538
rosa_multiflora Rmu_sc0000923.1_g000008 Rmu_sc0002931.1_g000001 Rmu_sc0006850.1_g000014
rosa_roxburghii Rroxscaffold_1G00021360 Rroxscaffold_1G00022610 Rroxscaffold_1G00022620 Rroxscaffold_1G00025730 Rroxscaffold_1G00029440 Rroxscaffold_1G00029610 Rroxscaffold_1G00032940 Rroxscaffold_1G00034000 Rroxscaffold_2G00087130 Rroxscaffold_2G00093110 Rroxscaffold_2G00097280 Rroxscaffold_2G00121440 Rroxscaffold_3G00225890 Rroxscaffold_3G00238130 Rroxscaffold_3G00241910 Rroxscaffold_3G00267280 Rroxscaffold_4G00295970 Rroxscaffold_4G00299040 Rroxscaffold_4G00303830 Rroxscaffold_4G00305880 Rroxscaffold_5G00335820 Rroxscaffold_5G00343740 Rroxscaffold_5G00346800 Rroxscaffold_5G00348110 Rroxscaffold_5G00371220 Rroxscaffold_5G00383470 Rroxscaffold_5G00387630 Rroxscaffold_6G00388100 Rroxscaffold_6G00395960 Rroxscaffold_6G00397500 Rroxscaffold_6G00405550 Rroxscaffold_7G00195020 Rroxscaffold_7G00217460
rosa_rugosa Rorug02G0515900 Rorug06G0038100.1
rosa_samantha Rh1CG171700 Rh2AG250300 Rh2AG481200 Rh2CG103900 Rh2CG467500 Rh2DG121800 Rh2DG258400 Rh3AG000900 Rh3CG000600 Rh3DG000800 Rh4DG164800 Rh5AG157100 Rh5AG476500 Rh5AG519300 Rh5DG192000 Rh5DG243900 Rh6CG399800 Rh6DG207500 Rh6DG458300 Rh6DG458400 Rh7AG314100 Rh7AG466900 Rh7DG095400
rosa_wichuraiana Rw5G004310 Rw5G049380 Rw6G000590 Rw6G025650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 444
AccII CGCG 2 cut(s) 25, 456
AciI CCGC 5 cut(s) 219, 222, 451, 454, 456
AclWI GGATC 3 cut(s) 82, 290, 343
AcoI YGGCCR 1 cut(s) 613
AcsI RAATTY 1 cut(s) 647
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 1 cut(s) 191
AgsI TTSAA 3 cut(s) 10, 55, 210
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 1 cut(s) 526
AluI AGCT 1 cut(s) 526
AlwI GGATC 3 cut(s) 82, 290, 343
AoxI GGCC 2 cut(s) 411, 613
ApeKI GCWGC 1 cut(s) 554
ApoI RAATTY 1 cut(s) 647
AspLEI GCGC 2 cut(s) 27, 66
AspS9I GGNCC 1 cut(s) 411
AsuHPI GGTGA 5 cut(s) 129, 308, 361, 577, 638
BalI TGGCCA 1 cut(s) 615
BbsI GAAGAC 3 cut(s) 36, 116, 245
BbvI GCAGC 1 cut(s) 541
BccI CCATC 6 cut(s) 136, 221, 340, 453, 556, 624
BceAI ACGGC 1 cut(s) 398
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BisI GCNGC 4 cut(s) 219, 222, 454, 555
BlsI GCNGC 4 cut(s) 220, 223, 455, 556
BmgT120I GGNCC 1 cut(s) 411
BmrI ACTGGG 1 cut(s) 580
BmsI GCATC 2 cut(s) 270, 323
BmuI ACTGGG 1 cut(s) 580
BpiI GAAGAC 3 cut(s) 36, 116, 245
BpmI CTGGAG 4 cut(s) 277, 286, 330, 383
Bsa29I ATCGAT 1 cut(s) 274
BsaJI CCNNGG 2 cut(s) 59, 454
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bse1I ACTGG 1 cut(s) 575
Bse3DI GCAATG 1 cut(s) 418
BseCI ATCGAT 1 cut(s) 274
BseDI CCNNGG 2 cut(s) 59, 454
BseGI GGATG 1 cut(s) 464
BseMI GCAATG 1 cut(s) 418
BseNI ACTGG 1 cut(s) 575
BseXI GCAGC 1 cut(s) 541
BseYI CCCAGC 1 cut(s) 408
Bsh1236I CGCG 2 cut(s) 25, 456
BshFI GGCC 2 cut(s) 413, 615
BshVI ATCGAT 1 cut(s) 274
BsiSI CCGG 2 cut(s) 178, 438
BsmI GAATGC 1 cut(s) 204
BsnI GGCC 2 cut(s) 413, 615
Bsp143I GATC 4 cut(s) 74, 169, 282, 335
Bsp19I CCATGG 1 cut(s) 59
BspACI CCGC 5 cut(s) 219, 222, 451, 454, 456
BspANI GGCC 2 cut(s) 413, 615
BspDI ATCGAT 1 cut(s) 274
BspFNI CGCG 2 cut(s) 25, 456
BspPI GGATC 3 cut(s) 82, 290, 343
BsrDI GCAATG 1 cut(s) 418
BsrI ACTGG 1 cut(s) 575
BssECI CCNNGG 2 cut(s) 59, 454
BssMI GATC 4 cut(s) 74, 169, 282, 335
BssNAI GTATAC 1 cut(s) 445
BssT1I CCWWGG 1 cut(s) 59
Bst1107I GTATAC 1 cut(s) 445
Bst4CI ACNGT 2 cut(s) 296, 638
BstC8I GCNNGC 1 cut(s) 613
BstDEI CTNAG 1 cut(s) 620
BstDSI CCRYGG 2 cut(s) 59, 454
BstF5I GGATG 1 cut(s) 464
BstFNI CGCG 2 cut(s) 25, 456
BstHHI GCGC 2 cut(s) 27, 66
BstKTI GATC 4 cut(s) 77, 172, 285, 338
BstMBI GATC 4 cut(s) 74, 169, 282, 335
BstNSI RCATGY 1 cut(s) 493
BstUI CGCG 2 cut(s) 25, 456
BstV1I GCAGC 1 cut(s) 541
BstV2I GAAGAC 3 cut(s) 36, 116, 245
BstX2I RGATCY 2 cut(s) 282, 335
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 2 cut(s) 282, 335
BstZ17I GTATAC 1 cut(s) 445
Bsu15I ATCGAT 1 cut(s) 274
BsuRI GGCC 2 cut(s) 413, 615
BsuTUI ATCGAT 1 cut(s) 274
BtgI CCRYGG 2 cut(s) 59, 454
BtgZI GCGATG 1 cut(s) 200
BtsCI GGATG 1 cut(s) 464
BtsIMutI CAGTG 1 cut(s) 634
Cac8I GCNNGC 1 cut(s) 613
CfoI GCGC 2 cut(s) 27, 66
Cfr13I GGNCC 1 cut(s) 411
Cfr42I CCGCGG 1 cut(s) 457
ClaI ATCGAT 1 cut(s) 274
Csp6I GTAC 1 cut(s) 190
CviAII CATG 8 cut(s) 60, 71, 104, 484, 490, 521, 533, 590
CviJI RGCY 6 cut(s) 58, 413, 526, 554, 575, 615
CviKI_1 RGCY 6 cut(s) 58, 413, 526, 554, 575, 615
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 1 cut(s) 620
DpnI GATC 4 cut(s) 76, 171, 284, 337
DpnII GATC 4 cut(s) 74, 169, 282, 335
EaeI YGGCCR 1 cut(s) 613
Eco130I CCWWGG 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 257
EcoT14I CCWWGG 1 cut(s) 59
ErhI CCWWGG 1 cut(s) 59
FaeI CATG 8 cut(s) 63, 74, 107, 487, 493, 524, 536, 593
FaiI YATR 9 cut(s) 61, 72, 105, 445, 485, 491, 522, 534, 591
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FatI CATG 8 cut(s) 59, 70, 103, 483, 489, 520, 532, 589
FauI CCCGC 1 cut(s) 458
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 4 cut(s) 219, 222, 454, 555
FokI GGATG 1 cut(s) 471
Fsp4HI GCNGC 4 cut(s) 219, 222, 454, 555
GlaI GCGC 2 cut(s) 26, 65
GluI GCNGC 4 cut(s) 219, 222, 454, 555
GsaI CCCAGC 1 cut(s) 412
GsuI CTGGAG 4 cut(s) 277, 286, 330, 383
HaeIII GGCC 2 cut(s) 413, 615
HapII CCGG 2 cut(s) 178, 438
HhaI GCGC 2 cut(s) 27, 66
Hin1II CATG 8 cut(s) 63, 74, 107, 487, 493, 524, 536, 593
Hin6I GCGC 2 cut(s) 25, 64
HinP1I GCGC 2 cut(s) 25, 64
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 4 cut(s) 206, 231, 604, 668
HpaII CCGG 2 cut(s) 178, 438
HphI GGTGA 5 cut(s) 129, 308, 361, 577, 638
Hpy166II GTNNAC 5 cut(s) 445, 505, 568, 641, 682
Hpy188I TCNGA 4 cut(s) 169, 292, 345, 561
Hpy188III TCNNGA 4 cut(s) 195, 235, 265, 672
Hpy8I GTNNAC 5 cut(s) 445, 505, 568, 641, 682
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 3 cut(s) 61, 122, 593
HpyCH4III ACNGT 2 cut(s) 296, 638
HpyCH4IV ACGT 1 cut(s) 643
HpyCH4V TGCA 1 cut(s) 493
HpyF3I CTNAG 1 cut(s) 620
HpySE526I ACGT 1 cut(s) 643
Hsp92II CATG 8 cut(s) 63, 74, 107, 487, 493, 524, 536, 593
HspAI GCGC 2 cut(s) 25, 64
KspI CCGCGG 1 cut(s) 457
Kzo9I GATC 4 cut(s) 74, 169, 282, 335
LmnI GCTCC 5 cut(s) 258, 311, 346, 364, 562
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 2 cut(s) 270, 323
MaeII ACGT 1 cut(s) 643
MalI GATC 4 cut(s) 76, 171, 284, 337
MboI GATC 4 cut(s) 74, 169, 282, 335
MboII GAAGA 8 cut(s) 36, 116, 123, 141, 250, 439, 442, 677
MflI RGATCY 2 cut(s) 282, 335
MlsI TGGCCA 1 cut(s) 615
MluCI AATT 4 cut(s) 528, 577, 647, 675
MluNI TGGCCA 1 cut(s) 615
MmeI TCCRAC 1 cut(s) 315
MnlI CCTC 4 cut(s) 44, 145, 150, 493
Mox20I TGGCCA 1 cut(s) 615
MscI TGGCCA 1 cut(s) 615
MseI TTAA 1 cut(s) 323
MslI CAYNNNNRTG 2 cut(s) 488, 621
Msp20I TGGCCA 1 cut(s) 615
MspA1I CMGCKG 1 cut(s) 456
MspI CCGG 2 cut(s) 178, 438
Mva1269I GAATGC 1 cut(s) 204
MvnI CGCG 2 cut(s) 25, 456
NcoI CCATGG 1 cut(s) 59
NdeII GATC 4 cut(s) 74, 169, 282, 335
NlaIII CATG 8 cut(s) 63, 74, 107, 487, 493, 524, 536, 593
NspI RCATGY 1 cut(s) 493
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 204
PfeI GAWTC 4 cut(s) 206, 231, 604, 668
PkrI GCNGC 4 cut(s) 220, 223, 455, 556
PspFI CCCAGC 1 cut(s) 408
PspPI GGNCC 1 cut(s) 411
PsuI RGATCY 2 cut(s) 282, 335
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
RseI CAYNNNNRTG 2 cut(s) 488, 621
SacII CCGCGG 1 cut(s) 457
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 4 cut(s) 219, 222, 454, 555
Sau3AI GATC 4 cut(s) 74, 169, 282, 335
Sau96I GGNCC 1 cut(s) 411
SetI ASST 5 cut(s) 53, 142, 504, 528, 646
SfaNI GCATC 2 cut(s) 270, 323
Sfr303I CCGCGG 1 cut(s) 457
SgrBI CCGCGG 1 cut(s) 457
SmiMI CAYNNNNRTG 2 cut(s) 488, 621
Sse9I AATT 4 cut(s) 528, 577, 647, 675
SsiI CCGC 5 cut(s) 219, 222, 451, 454, 456
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 2 cut(s) 296, 638
TaiI ACGT 1 cut(s) 646
TaqI TCGA 5 cut(s) 47, 152, 274, 327, 671
TasI AATT 4 cut(s) 528, 577, 647, 675
TatI WGTACW 1 cut(s) 189
TauI GCSGC 3 cut(s) 221, 224, 456
TfiI GAWTC 4 cut(s) 206, 231, 604, 668
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TscAI CASTG 1 cut(s) 641
TseI GCWGC 1 cut(s) 554
TspDTI ATGAA 5 cut(s) 104, 407, 500, 521, 575
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 1 cut(s) 641
XapI RAATTY 1 cut(s) 647
XceI RCATGY 1 cut(s) 493
XmiI GTMKAC 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.