Rh5AG157100

Mitochondrial inner membrane protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000640
Physical Location & Seq
Forward (+)
16616952 .. 16620067
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG157100.1

Sequence Viewer

Length: 432 bp
ATGGTGAAGTTTATGCTGGAGCATCTGCAGAAATCTGGGTGTGGGATATTAGACGGGTCTTGCTGGGCCGTTCATTGCGAGAAGAAGATTGCCGGAGTATACACCCGCCGCGGATGGATAATGGTGTGTAGTAATCACATGAACATGCAAGATGAGGTCAACCAAATTCGTGCTGGCCATCTTAGTGGTGATTGCCACTGTAAACGTGAATTTTTTCTGTGCCAAGAAGATTCGAGAATTGTGAACAAATTCCAGGCAGAAACAGGGTCTCCATACATAGCAGCCCACATTTTCAGGATTGAAGGAGCGGTTGCTGCTCATAATTTTGCTCCTGTGTTCATAATCTACAATGCTGGAACTGATATTCTAGATGGCGATCCTTTGGGCAGTCATCCATGGAGGAGAAGTGGAAGTTGCAGATATCATGCCTAG

Protein Analysis

143

Amino Acids

16.08

Weight (kDa)

7.64

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M76 PF09768 1 - 55 8.7e-07 Peptidase M76 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000640)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0331021 RchiOBHm_Chr1g0335951 RchiOBHm_Chr1g0338001 RchiOBHm_Chr6g0276241 RchiOBHm_Chr6g0297731 RchiOBHm_Chr7g0188211 RchiOBHm_Chr7g0217921 RchiOBHm_Chr7g0238211
rosa_laevigata RLG00000007457 RLG00000015447 RLG00000019185 RLG00000032916 RLG00000034538
rosa_multiflora Rmu_sc0000923.1_g000008 Rmu_sc0002931.1_g000001 Rmu_sc0006850.1_g000014
rosa_roxburghii Rroxscaffold_1G00021360 Rroxscaffold_1G00022610 Rroxscaffold_1G00022620 Rroxscaffold_1G00025730 Rroxscaffold_1G00029440 Rroxscaffold_1G00029610 Rroxscaffold_1G00032940 Rroxscaffold_1G00034000 Rroxscaffold_2G00087130 Rroxscaffold_2G00093110 Rroxscaffold_2G00097280 Rroxscaffold_2G00121440 Rroxscaffold_3G00225890 Rroxscaffold_3G00238130 Rroxscaffold_3G00241910 Rroxscaffold_3G00267280 Rroxscaffold_4G00295970 Rroxscaffold_4G00299040 Rroxscaffold_4G00303830 Rroxscaffold_4G00305880 Rroxscaffold_5G00335820 Rroxscaffold_5G00343740 Rroxscaffold_5G00346800 Rroxscaffold_5G00348110 Rroxscaffold_5G00371220 Rroxscaffold_5G00383470 Rroxscaffold_5G00387630 Rroxscaffold_6G00388100 Rroxscaffold_6G00395960 Rroxscaffold_6G00397500 Rroxscaffold_6G00405550 Rroxscaffold_7G00195020 Rroxscaffold_7G00217460
rosa_rugosa Rorug02G0515900 Rorug06G0038100.1
rosa_samantha Rh1CG171700 Rh2AG250300 Rh2AG481200 Rh2CG103900 Rh2CG467500 Rh2DG121800 Rh2DG258400 Rh3AG000900 Rh3CG000600 Rh3DG000800 Rh4DG164800 Rh5AG157100 Rh5AG476500 Rh5AG519300 Rh5DG192000 Rh5DG243900 Rh6CG399800 Rh6DG207500 Rh6DG458300 Rh6DG458400 Rh7AG314100 Rh7AG466900 Rh7DG095400
rosa_wichuraiana Rw5G004310 Rw5G049380 Rw6G000590 Rw6G025650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 99
AccII CGCG 1 cut(s) 111
AciI CCGC 4 cut(s) 106, 109, 111, 308
AclWI GGATC 1 cut(s) 371
AcoI YGGCCR 1 cut(s) 175
AcsI RAATTY 3 cut(s) 165, 209, 248
AgsI TTSAA 1 cut(s) 302
AjnI CCWGG 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 273
AlwI GGATC 1 cut(s) 371
AoxI GGCC 2 cut(s) 66, 175
ApeKI GCWGC 2 cut(s) 281, 314
ApoI RAATTY 3 cut(s) 165, 209, 248
Asp700I GAANNNNTTC 2 cut(s) 213, 248
AspS9I GGNCC 1 cut(s) 66
AsuHPI GGTGA 2 cut(s) 16, 200
BalI TGGCCA 1 cut(s) 177
BbvI GCAGC 2 cut(s) 293, 301
BccI CCATC 3 cut(s) 108, 186, 365
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 254
BcoDI GTCTC 1 cut(s) 273
BfaI CTAG 2 cut(s) 368, 430
BfmI CTRYAG 1 cut(s) 26
BisI GCNGC 3 cut(s) 109, 282, 315
BlsI GCNGC 3 cut(s) 110, 283, 316
Bme1390I CCNGG 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 66
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 1 cut(s) 31
BpmI CTGGAG 1 cut(s) 38
BsaBI GATNNNNATC 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 273
BsaJI CCNNGG 2 cut(s) 109, 395
BsaXI ACNNNNNCTCC 4 cut(s) 253, 283, 391, 421
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 375
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 2 cut(s) 109, 395
BseGI GGATG 2 cut(s) 119, 391
BseJI GATNNNNATC 1 cut(s) 375
BseMI GCAATG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 415
BseXI GCAGC 2 cut(s) 293, 301
BseYI CCCAGC 1 cut(s) 63
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 68, 177
BsiSI CCGG 1 cut(s) 93
BsmAI GTCTC 1 cut(s) 273
BsnI GGCC 2 cut(s) 68, 177
Bso31I GGTCTC 1 cut(s) 273
Bsp143I GATC 1 cut(s) 376
Bsp19I CCATGG 1 cut(s) 395
BspACI CCGC 4 cut(s) 106, 109, 111, 308
BspANI GGCC 2 cut(s) 68, 177
BspFNI CGCG 1 cut(s) 111
BspMAI CTGCAG 1 cut(s) 30
BspPI GGATC 1 cut(s) 371
BspTNI GGTCTC 1 cut(s) 273
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 73
BssECI CCNNGG 2 cut(s) 109, 395
BssMI GATC 1 cut(s) 376
BssNAI GTATAC 1 cut(s) 100
BssT1I CCWWGG 1 cut(s) 395
Bst1107I GTATAC 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 200
BstC8I GCNNGC 1 cut(s) 175
BstDEI CTNAG 1 cut(s) 182
BstDSI CCRYGG 2 cut(s) 109, 395
BstF5I GGATG 2 cut(s) 119, 391
BstFNI CGCG 1 cut(s) 111
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 1 cut(s) 273
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNI CCWGG 1 cut(s) 254
BstNSI RCATGY 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 252
BstSFI CTRYAG 1 cut(s) 26
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 2 cut(s) 293, 301
BstXI CCANNNNNNTGG 1 cut(s) 185
BstZ17I GTATAC 1 cut(s) 100
BsuRI GGCC 2 cut(s) 68, 177
BtgI CCRYGG 2 cut(s) 109, 395
BtsCI GGATG 2 cut(s) 119, 391
BtsIMutI CAGTG 1 cut(s) 196
Cac8I GCNNGC 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 66
Cfr42I CCGCGG 1 cut(s) 112
CviAII CATG 4 cut(s) 139, 145, 396, 425
CviJI RGCY 3 cut(s) 68, 177, 284
CviKI_1 RGCY 3 cut(s) 68, 177, 284
DdeI CTNAG 1 cut(s) 182
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
EaeI YGGCCR 1 cut(s) 175
Eco130I CCWWGG 1 cut(s) 395
Eco31I GGTCTC 1 cut(s) 273
Eco32I GATATC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 252
EcoRV GATATC 1 cut(s) 422
EcoT14I CCWWGG 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 395
FaeI CATG 4 cut(s) 142, 148, 399, 428
FatI CATG 4 cut(s) 138, 144, 395, 424
FauI CCCGC 1 cut(s) 113
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 3 cut(s) 109, 282, 315
FokI GGATG 2 cut(s) 126, 378
Fsp4HI GCNGC 3 cut(s) 109, 282, 315
FspBI CTAG 2 cut(s) 368, 430
GluI GCNGC 3 cut(s) 109, 282, 315
GsaI CCCAGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 2 cut(s) 68, 177
HapII CCGG 1 cut(s) 93
Hin1II CATG 4 cut(s) 142, 148, 399, 428
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 1 cut(s) 230
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 16, 200
Hpy166II GTNNAC 4 cut(s) 100, 160, 203, 244
Hpy188III TCNNGA 3 cut(s) 234, 295, 368
Hpy8I GTNNAC 4 cut(s) 100, 160, 203, 244
HpyAV CCTTC 1 cut(s) 296
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 3 cut(s) 28, 148, 417
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 1 cut(s) 182
HpySE526I ACGT 1 cut(s) 205
Hsp92II CATG 4 cut(s) 142, 148, 399, 428
KspI CCGCGG 1 cut(s) 112
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 3 cut(s) 19, 305, 334
Lsp1109I GCAGC 2 cut(s) 293, 301
LweI GCATC 1 cut(s) 31
MaeI CTAG 2 cut(s) 368, 430
MaeII ACGT 1 cut(s) 205
MalI GATC 1 cut(s) 378
MbiI CCGCTC 1 cut(s) 308
MboI GATC 1 cut(s) 376
MboII GAAGA 3 cut(s) 94, 97, 239
MlsI TGGCCA 1 cut(s) 177
MluCI AATT 5 cut(s) 165, 209, 237, 248, 322
MluNI TGGCCA 1 cut(s) 177
MnlI CCTC 2 cut(s) 148, 393
Mox20I TGGCCA 1 cut(s) 177
MroXI GAANNNNTTC 2 cut(s) 213, 248
MscI TGGCCA 1 cut(s) 177
MslI CAYNNNNRTG 2 cut(s) 143, 183
Msp20I TGGCCA 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 254
MvaI CCWGG 1 cut(s) 254
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 314
NcoI CCATGG 1 cut(s) 395
NdeII GATC 1 cut(s) 376
NlaIII CATG 4 cut(s) 142, 148, 399, 428
NspI RCATGY 1 cut(s) 148
PdmI GAANNNNTTC 2 cut(s) 213, 248
PfeI GAWTC 1 cut(s) 230
PkrI GCNGC 3 cut(s) 110, 283, 316
Psp6I CCWGG 1 cut(s) 252
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 1 cut(s) 252
PspPI GGNCC 1 cut(s) 66
PstI CTGCAG 1 cut(s) 30
RseI CAYNNNNRTG 2 cut(s) 143, 183
SacII CCGCGG 1 cut(s) 112
SatI GCNGC 3 cut(s) 109, 282, 315
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 254
SetI ASST 2 cut(s) 159, 208
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 1 cut(s) 26
Sfr303I CCGCGG 1 cut(s) 112
SgrBI CCGCGG 1 cut(s) 112
SmiMI CAYNNNNRTG 2 cut(s) 143, 183
Sse9I AATT 5 cut(s) 165, 209, 237, 248, 322
SsiI CCGC 4 cut(s) 106, 109, 111, 308
SspMI CTAG 2 cut(s) 368, 430
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 1 cut(s) 395
TaaI ACNGT 1 cut(s) 200
TaiI ACGT 1 cut(s) 208
TaqI TCGA 1 cut(s) 233
TasI AATT 5 cut(s) 165, 209, 237, 248, 322
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 1 cut(s) 230
TscAI CASTG 1 cut(s) 203
TseI GCWGC 2 cut(s) 281, 314
TspDTI ATGAA 3 cut(s) 62, 155, 328
TspRI CASTG 1 cut(s) 203
XapI RAATTY 3 cut(s) 165, 209, 248
XbaI TCTAGA 1 cut(s) 367
XceI RCATGY 1 cut(s) 148
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 99
XmnI GAANNNNTTC 2 cut(s) 213, 248
XspI CTAG 2 cut(s) 368, 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.