RLG00000029163

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001119
Physical Location & Seq
Reverse (-)
33902695 .. 33903170
476 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029163

Sequence Viewer

Length: 396 bp
ATGGCTGGTGCCGCAAATGGGATCGCAGCGCTGTCCTTTTCCGGTGTGTTTTACGTCAACCGAGGGAGTGCTAGCGGTTGTCTTTTTTTGCTTTTCCTTCTCCTTGTACTTGCTGGCTGCAGCAATGGAGGAGGCATGATGGATTTAGATATGATTTTAGTTCCTGCTGGGAGGACCGATGGAGGTGCCAGCAATCCTTGGAGTTGGGTCGCCGGTGGGCATGGGATCGATGGTGGCTGGTTAGGCAATGGCATGAACTGCAACCTAGCCCGGAAGATGGAATCTAAAGCCTTGAGACGCCCAAGCTTTTCGGCGACGGCACAAGGGCACAGTGGCGGAAGCTTCTCAACAGCAGTGCAAGCTCTCTGTGGTGGAGGCCTTGCAGTGACTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

13.04

Weight (kDa)

8.49

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 8, 185
AciI CCGC 3 cut(s) 12, 75, 336
AclWI GGATC 2 cut(s) 29, 233
AcyI GRCGYC 1 cut(s) 298
AfaI GTAC 1 cut(s) 108
AfeI AGCGCT 1 cut(s) 30
AfiI CCNNNNNNNGG 2 cut(s) 18, 277
AluBI AGCT 3 cut(s) 306, 342, 362
AluI AGCT 3 cut(s) 306, 342, 362
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 2 cut(s) 29, 233
AlwNI CAGNNNCTG 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 1 cut(s) 376
ApeKI GCWGC 3 cut(s) 26, 117, 120
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 174
AsuC2I CCSGG 1 cut(s) 271
AsuNHI GCTAGC 1 cut(s) 71
AvaII GGWCC 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 330
BanI GGYRCC 2 cut(s) 8, 185
BbvI GCAGC 3 cut(s) 38, 104, 132
BccI CCATC 4 cut(s) 133, 173, 224, 271
BceAI ACGGC 1 cut(s) 333
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BcnI CCSGG 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 2 cut(s) 72, 266
BfmI CTRYAG 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 32
BisI GCNGC 4 cut(s) 12, 27, 118, 121
BlsI GCNGC 4 cut(s) 13, 28, 119, 122
Bme1390I CCNGG 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 2 cut(s) 10, 187
BmrFI CCNGG 1 cut(s) 271
BmtI GCTAGC 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 313
BpuMI CCSGG 1 cut(s) 271
Bsa29I ATCGAT 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 298
BsaJI CCNNGG 2 cut(s) 61, 197
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 277
Bse118I RCCGGY 1 cut(s) 212
Bse3DI GCAATG 2 cut(s) 130, 253
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 61, 197
BseLI CCNNNNNNNGG 2 cut(s) 18, 277
BseMI GCAATG 2 cut(s) 130, 253
BseRI GAGGAG 1 cut(s) 144
BseSI GKGCMC 1 cut(s) 330
BseXI GCAGC 3 cut(s) 38, 104, 132
BseYI CCCAGC 1 cut(s) 167
BshFI GGCC 1 cut(s) 378
BshNI GGYRCC 2 cut(s) 8, 185
BshVI ATCGAT 1 cut(s) 228
BsiSI CCGG 3 cut(s) 42, 213, 271
BslI CCNNNNNNNGG 2 cut(s) 18, 277
BsmAI GTCTC 1 cut(s) 289
BsmBI CGTCTC 1 cut(s) 289
BsnI GGCC 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 2 cut(s) 21, 225
BspACI CCGC 3 cut(s) 12, 75, 336
BspANI GGCC 1 cut(s) 378
BspDI ATCGAT 1 cut(s) 228
BspLI GGNNCC 2 cut(s) 10, 187
BspMAI CTGCAG 1 cut(s) 122
BspOI GCTAGC 1 cut(s) 75
BspPI GGATC 2 cut(s) 29, 233
BspT107I GGYRCC 2 cut(s) 8, 185
BsrDI GCAATG 2 cut(s) 130, 253
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 2 cut(s) 61, 197
BssMI GATC 2 cut(s) 21, 225
BssNI GRCGYC 1 cut(s) 298
BssT1I CCWWGG 1 cut(s) 197
Bst4CI ACNGT 1 cut(s) 332
BstACI GRCGYC 1 cut(s) 298
BstAPI GCANNNNNTGC 2 cut(s) 258, 389
BstC8I GCNNGC 4 cut(s) 73, 115, 190, 360
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 2 cut(s) 24, 228
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 2 cut(s) 21, 225
BstMWI GCNNNNNNNGC 5 cut(s) 11, 243, 258, 359, 389
BstSCI CCNGG 1 cut(s) 269
BstSFI CTRYAG 1 cut(s) 118
BstSLI GKGCMC 1 cut(s) 330
BstV1I GCAGC 3 cut(s) 38, 104, 132
Bsu15I ATCGAT 1 cut(s) 228
BsuRI GGCC 1 cut(s) 378
BsuTUI ATCGAT 1 cut(s) 228
BtsI GCAGTG 2 cut(s) 360, 390
BtsIMutI CAGTG 3 cut(s) 337, 360, 390
Cac8I GCNNGC 4 cut(s) 73, 115, 190, 360
CaiI CAGNNNCTG 1 cut(s) 389
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 174
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 1 cut(s) 306
Csp6I GTAC 1 cut(s) 107
CviAII CATG 3 cut(s) 136, 221, 253
CviJI RGCY 9 cut(s) 5, 117, 237, 269, 290, 306, 342, 362, 378
CviKI_1 RGCY 9 cut(s) 5, 117, 237, 269, 290, 306, 342, 362, 378
CviQI GTAC 1 cut(s) 107
DpnI GATC 2 cut(s) 23, 227
DpnII GATC 2 cut(s) 21, 225
EciI GGCGGA 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 197
Eco147I AGGCCT 1 cut(s) 378
Eco47I GGWCC 1 cut(s) 174
Eco47III AGCGCT 1 cut(s) 30
EcoT14I CCWWGG 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 197
Esp3I CGTCTC 1 cut(s) 289
FaeI CATG 3 cut(s) 139, 224, 256
FaiI YATR 4 cut(s) 137, 152, 222, 254
FatI CATG 3 cut(s) 135, 220, 252
Fnu4HI GCNGC 4 cut(s) 12, 27, 118, 121
Fsp4HI GCNGC 4 cut(s) 12, 27, 118, 121
FspBI CTAG 2 cut(s) 72, 266
GlaI GCGC 1 cut(s) 30
GluI GCNGC 4 cut(s) 12, 27, 118, 121
GsaI CCCAGC 1 cut(s) 171
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 1 cut(s) 378
HapII CCGG 3 cut(s) 42, 213, 271
HgaI GACGC 1 cut(s) 306
HhaI GCGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 298
Hin1II CATG 3 cut(s) 139, 224, 256
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 2 cut(s) 304, 340
HinfI GANTC 1 cut(s) 281
HpaII CCGG 3 cut(s) 42, 213, 271
Hpy166II GTNNAC 1 cut(s) 58
Hpy8I GTNNAC 1 cut(s) 58
Hpy99I CGWCG 1 cut(s) 319
HpyAV CCTTC 1 cut(s) 107
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 4 cut(s) 120, 261, 358, 383
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 243, 258, 359, 389
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 1 cut(s) 298
Hsp92II CATG 3 cut(s) 139, 224, 256
HspAI GCGC 1 cut(s) 29
Kzo9I GATC 2 cut(s) 21, 225
LpnPI CCDG 8 cut(s) 55, 99, 153, 177, 202, 223, 226, 284
Lsp1109I GCAGC 3 cut(s) 38, 104, 132
MaeI CTAG 2 cut(s) 72, 266
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 385
MalI GATC 2 cut(s) 23, 227
MboI GATC 2 cut(s) 21, 225
MboII GAAGA 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 330
MnlI CCTC 6 cut(s) 56, 122, 125, 165, 176, 368
MspI CCGG 3 cut(s) 42, 213, 271
MspR9I CCNGG 1 cut(s) 271
MwoI GCNNNNNNNGC 5 cut(s) 11, 243, 258, 359, 389
NciI CCSGG 1 cut(s) 271
NdeII GATC 2 cut(s) 21, 225
NheI GCTAGC 1 cut(s) 71
NlaIII CATG 3 cut(s) 139, 224, 256
NlaIV GGNNCC 2 cut(s) 10, 187
NmuCI GTSAC 1 cut(s) 385
PceI AGGCCT 1 cut(s) 378
PfeI GAWTC 1 cut(s) 281
PkrI GCNGC 4 cut(s) 13, 28, 119, 122
PspFI CCCAGC 1 cut(s) 167
PspN4I GGNNCC 2 cut(s) 10, 187
PspPI GGNCC 1 cut(s) 174
PstI CTGCAG 1 cut(s) 122
PstNI CAGNNNCTG 1 cut(s) 389
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SatI GCNGC 4 cut(s) 12, 27, 118, 121
Sau3AI GATC 2 cut(s) 21, 225
Sau96I GGNCC 1 cut(s) 174
ScrFI CCNGG 1 cut(s) 271
SduI GDGCHC 1 cut(s) 330
SetI ASST 6 cut(s) 57, 187, 267, 308, 344, 364
SfcI CTRYAG 1 cut(s) 118
SgrAI CRCCGGYG 1 cut(s) 212
SinI GGWCC 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
SseBI AGGCCT 1 cut(s) 378
SsiI CCGC 3 cut(s) 12, 75, 336
SspMI CTAG 2 cut(s) 72, 266
StuI AGGCCT 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 269
StyI CCWWGG 1 cut(s) 197
TaaI ACNGT 1 cut(s) 332
TaiI ACGT 1 cut(s) 57
TaqI TCGA 1 cut(s) 228
TaqII GACCGA 1 cut(s) 191
TatI WGTACW 1 cut(s) 106
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 1 cut(s) 281
TscAI CASTG 3 cut(s) 337, 360, 390
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 3 cut(s) 26, 117, 120
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 1 cut(s) 269
TspRI CASTG 3 cut(s) 337, 360, 390
VpaK11BI GGWCC 1 cut(s) 174
XspI CTAG 2 cut(s) 72, 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.