Rh3DG236000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001119
Physical Location & Seq
Forward (+)
22540558 .. 22542099
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG236000.1

Sequence Viewer

Length: 738 bp
ATGGCTGGTGCCACAAATGGGATCGCAGCGCTGTCCTTTCCCGGCGTGGTTTACGTCAACCGGGGGCGTGCCAGTGGTCATTTTTTTCTAGTTTTCCTTCTCTTTGTACTTGCTGGTTGCAGCGTGATGGATCTGGATTTGCTTTTGGTTCCTGCTGGGAGGGTCGACGGAGGTGCCATCGATCCTTGGAGGTGGAGGGGGTTCCGGCTATCAGGGTCTAGCGTCGTCGATATCGGAGGCGTGGATGCCAGTGTCAGTGCTTGGGTGGGTTGCGGGGCCGCAGGTGGGCAAGGGATCGATCGTGGCTGGTTTGTTGATGGCCTGAATTCCAACATAGCCCGGCTGATGGAATCTGAAGCCTTGAGATGGCGAAGCTTCCCGATGACTAGGGCGGACATGGTCGTTGATTTGGATGATAGATGGTTGGGTTTCAAGGGTTTCAAGCCAGATTTTGCTGGTTCTGGTTTGAAGGACGGGGTGGATGATTGCAATGGTCGTACTGATGTTAGACATGGAGGTTCGCTGCAATGTCGAAGAGTTGCCGGTGTTGGGTTAGCCTGGCTAGATCAGATGCAAAATGCCACAAGTTACAAAATTAATCACGTTTTAGACTATCTGGTGAATGCGGACAAAACCGACACATCTCTTGAAAAGGTTGAAGGGATTAAAAATAGGAGCACAACAACTGAATCCAACTTCATGATCATTATGTCCTTAAATCATAACAAGATGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

245

Amino Acids

26.56

Weight (kDa)

6.41

Isoelectric Point (pI)

25.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 272
Acc36I ACCTGC 1 cut(s) 272
AccB1I GGYRCC 2 cut(s) 8, 173
AccI GTMKAC 1 cut(s) 165
AciI CCGC 4 cut(s) 273, 279, 392, 626
AclWI GGATC 4 cut(s) 29, 138, 176, 302
AcsI RAATTY 1 cut(s) 325
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 2 cut(s) 108, 499
AfeI AGCGCT 1 cut(s) 30
AfiI CCNNNNNNNGG 5 cut(s) 18, 285, 346, 366, 549
AgsI TTSAA 5 cut(s) 433, 442, 469, 650, 659
AjnI CCWGG 1 cut(s) 557
AluBI AGCT 1 cut(s) 375
AluI AGCT 1 cut(s) 375
Alw21I GWGCWC 1 cut(s) 680
AlwI GGATC 4 cut(s) 29, 138, 176, 302
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 2 cut(s) 276, 319
ApeKI GCWGC 3 cut(s) 26, 120, 523
ApoI RAATTY 1 cut(s) 325
AseI ATTAAT 1 cut(s) 597
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 276
AsuC2I CCSGG 3 cut(s) 42, 62, 340
AsuHPI GGTGA 1 cut(s) 631
BanI GGYRCC 2 cut(s) 8, 173
Bbv12I GWGCWC 1 cut(s) 680
BbvI GCAGC 3 cut(s) 38, 132, 510
BccI CCATC 6 cut(s) 121, 185, 311, 340, 360, 414
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 1 cut(s) 559
BclI TGATCA 1 cut(s) 702
BcnI CCSGG 3 cut(s) 42, 62, 340
BfaI CTAG 4 cut(s) 89, 219, 387, 563
BfoI RGCGCY 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 4 cut(s) 27, 121, 279, 524
BlsI GCNGC 4 cut(s) 28, 122, 280, 525
Bme1390I CCNGG 4 cut(s) 42, 62, 340, 559
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 5 cut(s) 10, 150, 175, 203, 277
BmrFI CCNGG 4 cut(s) 42, 62, 340, 559
BmsI GCATC 2 cut(s) 235, 561
BpuEI CTTGAG 1 cut(s) 382
BpuMI CCSGG 3 cut(s) 42, 62, 340
Bsa29I ATCGAT 2 cut(s) 180, 297
BsaJI CCNNGG 2 cut(s) 61, 185
Bsc4I CCNNNNNNNGG 5 cut(s) 18, 285, 346, 366, 549
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 2 cut(s) 72, 249
Bse3DI GCAATG 2 cut(s) 496, 533
BseBI CCWGG 1 cut(s) 559
BseCI ATCGAT 2 cut(s) 180, 297
BseDI CCNNGG 2 cut(s) 61, 185
BseGI GGATG 3 cut(s) 250, 418, 487
BseLI CCNNNNNNNGG 5 cut(s) 18, 285, 346, 366, 549
BseMI GCAATG 2 cut(s) 496, 533
BseNI ACTGG 2 cut(s) 72, 249
BseXI GCAGC 3 cut(s) 38, 132, 510
BseYI CCCAGC 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 2 cut(s) 278, 321
BshNI GGYRCC 2 cut(s) 8, 173
BshVI ATCGAT 2 cut(s) 180, 297
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 680
BsiSI CCGG 5 cut(s) 42, 61, 205, 340, 543
BslI CCNNNNNNNGG 5 cut(s) 18, 285, 346, 366, 549
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 2 cut(s) 278, 321
Bsp1286I GDGCHC 1 cut(s) 680
Bsp143I GATC 7 cut(s) 21, 130, 181, 294, 298, 565, 702
BspACI CCGC 4 cut(s) 273, 279, 392, 626
BspANI GGCC 2 cut(s) 278, 321
BspDI ATCGAT 2 cut(s) 180, 297
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 5 cut(s) 10, 150, 175, 203, 277
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 4 cut(s) 29, 138, 176, 302
BspT107I GGYRCC 2 cut(s) 8, 173
BsrDI GCAATG 2 cut(s) 496, 533
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 2 cut(s) 72, 249
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 2 cut(s) 61, 185
BssMI GATC 7 cut(s) 21, 130, 181, 294, 298, 565, 702
BssT1I CCWWGG 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 559
Bst6I CTCTTC 1 cut(s) 529
BstC8I GCNNGC 1 cut(s) 69
BstF5I GGATG 3 cut(s) 250, 418, 487
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 7 cut(s) 24, 133, 184, 297, 301, 568, 705
BstMBI GATC 7 cut(s) 21, 130, 181, 294, 298, 565, 702
BstMCI CGRYCG 1 cut(s) 301
BstNI CCWGG 1 cut(s) 559
BstSCI CCNGG 4 cut(s) 40, 60, 338, 557
BstV1I GCAGC 3 cut(s) 38, 132, 510
BstX2I RGATCY 1 cut(s) 130
BstYI RGATCY 1 cut(s) 130
Bsu15I ATCGAT 2 cut(s) 180, 297
BsuRI GGCC 2 cut(s) 278, 321
BsuTUI ATCGAT 2 cut(s) 180, 297
BtsCI GGATG 3 cut(s) 250, 418, 487
BtsIMutI CAGTG 3 cut(s) 79, 256, 262
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 69
CciI TCATGA 1 cut(s) 699
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 276
ClaI ATCGAT 2 cut(s) 180, 297
CseI GACGC 1 cut(s) 211
Csp6I GTAC 2 cut(s) 107, 498
CviAII CATG 3 cut(s) 397, 512, 700
CviQI GTAC 2 cut(s) 107, 498
DpnI GATC 7 cut(s) 23, 132, 183, 296, 300, 567, 704
DpnII GATC 7 cut(s) 21, 130, 181, 294, 298, 565, 702
Eam1104I CTCTTC 1 cut(s) 529
EarI CTCTTC 1 cut(s) 529
EciI GGCGGA 1 cut(s) 407
Eco130I CCWWGG 1 cut(s) 185
Eco32I GATATC 1 cut(s) 232
Eco47III AGCGCT 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 557
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 185
FaeI CATG 3 cut(s) 400, 515, 703
FaiI YATR 6 cut(s) 335, 398, 513, 701, 710, 723
FatI CATG 3 cut(s) 396, 511, 699
FauI CCCGC 1 cut(s) 266
FbaI TGATCA 1 cut(s) 702
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 4 cut(s) 27, 121, 279, 524
FokI GGATG 3 cut(s) 257, 425, 494
Fsp4HI GCNGC 4 cut(s) 27, 121, 279, 524
FspBI CTAG 4 cut(s) 89, 219, 387, 563
GlaI GCGC 1 cut(s) 30
GluI GCNGC 4 cut(s) 27, 121, 279, 524
GsaI CCCAGC 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 278, 321
HapII CCGG 5 cut(s) 42, 61, 205, 340, 543
HgaI GACGC 1 cut(s) 211
HhaI GCGC 1 cut(s) 31
Hin1II CATG 3 cut(s) 400, 515, 703
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 2 cut(s) 58, 166
HindII GTYRAC 2 cut(s) 58, 166
HindIII AAGCTT 1 cut(s) 373
HinfI GANTC 2 cut(s) 350, 689
HpaII CCGG 5 cut(s) 42, 61, 205, 340, 543
HphI GGTGA 1 cut(s) 631
Hpy166II GTNNAC 3 cut(s) 52, 58, 166
Hpy188I TCNGA 3 cut(s) 236, 355, 570
Hpy188III TCNNGA 4 cut(s) 134, 379, 647, 700
Hpy8I GTNNAC 3 cut(s) 52, 58, 166
Hpy99I CGWCG 3 cut(s) 170, 227, 230
HpyAV CCTTC 3 cut(s) 107, 463, 653
HpyCH4IV ACGT 2 cut(s) 54, 603
HpyCH4V TGCA 4 cut(s) 120, 489, 526, 574
HpySE526I ACGT 2 cut(s) 54, 603
Hsp92II CATG 3 cut(s) 400, 515, 703
HspAI GCGC 1 cut(s) 29
Ksp22I TGATCA 1 cut(s) 702
Kzo9I GATC 7 cut(s) 21, 130, 181, 294, 298, 565, 702
LmnI GCTCC 1 cut(s) 675
Lsp1109I GCAGC 3 cut(s) 38, 132, 510
LweI GCATC 2 cut(s) 235, 561
MaeI CTAG 4 cut(s) 89, 219, 387, 563
MaeII ACGT 2 cut(s) 54, 603
MaeIII GTNAC 1 cut(s) 587
MalI GATC 7 cut(s) 23, 132, 183, 296, 300, 567, 704
MboI GATC 7 cut(s) 21, 130, 181, 294, 298, 565, 702
MboII GAAGA 1 cut(s) 546
MflI RGATCY 1 cut(s) 130
MhlI GDGCHC 1 cut(s) 680
MluCI AATT 2 cut(s) 325, 594
MmeI TCCRAC 2 cut(s) 354, 717
MnlI CCTC 6 cut(s) 153, 164, 183, 189, 230, 509
MseI TTAA 4 cut(s) 597, 666, 716, 736
MspI CCGG 5 cut(s) 42, 61, 205, 340, 543
MspR9I CCNGG 4 cut(s) 42, 62, 340, 559
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 1 cut(s) 559
NciI CCSGG 3 cut(s) 42, 62, 340
NdeII GATC 7 cut(s) 21, 130, 181, 294, 298, 565, 702
NlaIII CATG 3 cut(s) 400, 515, 703
NlaIV GGNNCC 5 cut(s) 10, 150, 175, 203, 277
PagI TCATGA 1 cut(s) 699
PaqCI CACCTGC 1 cut(s) 272
PctI GAATGC 1 cut(s) 628
PfeI GAWTC 2 cut(s) 350, 689
PflFI GACNNNGTC 1 cut(s) 398
PkrI GCNGC 4 cut(s) 28, 122, 280, 525
Ple19I CGATCG 1 cut(s) 301
PshBI ATTAAT 1 cut(s) 597
Psp6I CCWGG 1 cut(s) 557
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 1 cut(s) 557
PspN4I GGNNCC 5 cut(s) 10, 150, 175, 203, 277
PspPI GGNCC 1 cut(s) 276
PsuI RGATCY 1 cut(s) 130
PsyI GACNNNGTC 1 cut(s) 398
PvuI CGATCG 1 cut(s) 301
RsaI GTAC 2 cut(s) 108, 499
RsaNI GTAC 2 cut(s) 107, 498
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 4 cut(s) 597, 666, 716, 736
SatI GCNGC 4 cut(s) 27, 121, 279, 524
Sau3AI GATC 7 cut(s) 21, 130, 181, 294, 298, 565, 702
Sau96I GGNCC 1 cut(s) 276
ScrFI CCNGG 4 cut(s) 42, 62, 340, 559
SduI GDGCHC 1 cut(s) 680
SetI ASST 8 cut(s) 57, 175, 194, 286, 377, 520, 606, 657
SfaNI GCATC 2 cut(s) 235, 561
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
Sse9I AATT 2 cut(s) 325, 594
SsiI CCGC 4 cut(s) 273, 279, 392, 626
SspMI CTAG 4 cut(s) 89, 219, 387, 563
StyD4I CCNGG 4 cut(s) 40, 60, 338, 557
StyI CCWWGG 1 cut(s) 185
TaiI ACGT 2 cut(s) 57, 606
TaqI TCGA 5 cut(s) 165, 180, 228, 297, 532
TasI AATT 2 cut(s) 325, 594
TatI WGTACW 1 cut(s) 106
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 2 cut(s) 350, 689
Tru1I TTAA 4 cut(s) 597, 666, 716, 736
Tru9I TTAA 4 cut(s) 597, 666, 716, 736
TscAI CASTG 3 cut(s) 79, 256, 262
TseI GCWGC 3 cut(s) 26, 120, 523
TspDTI ATGAA 1 cut(s) 688
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 3 cut(s) 79, 256, 262
Tth111I GACNNNGTC 1 cut(s) 398
VspI ATTAAT 1 cut(s) 597
XapI RAATTY 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 4 cut(s) 89, 219, 387, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.