Rh5BG251900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001119
Physical Location & Seq
Forward (+)
31109269 .. 31109751
483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG251900.1

Sequence Viewer

Length: 276 bp
ATGGTCATGGCTATTGCTAGTCTGGTGGCTAAGCGTTTCTTTCTTATTGTTGCTTTTGTTTTGTTGCTTGGAGCATCGCCTTGGTGTGGGTTCCCTATGGTTGTTGTTTGGGACGGTAGTTTTGATGTTTGGGAGGCAGATCTGAGGCCGAGCTCTATCGGCGTGGCTTTGGTTTGGTCTGAATTGGATCGGCGCGACTGGGTTTTGGATTGTGGTCGTCAAACTACCGACGGTGGTGGGGATTGCACAATCGCTGTGGTTGGTGGGTGGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

9.94

Weight (kDa)

4.36

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 195
AclWI GGATC 1 cut(s) 195
AfiI CCNNNNNNNGG 1 cut(s) 86
AluBI AGCT 1 cut(s) 153
AluI AGCT 1 cut(s) 153
Alw21I GWGCWC 1 cut(s) 155
AlwI GGATC 1 cut(s) 195
AoxI GGCC 1 cut(s) 146
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AspLEI GCGC 1 cut(s) 195
BanII GRGCYC 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 155
BfaI CTAG 1 cut(s) 18
BglII AGATCT 1 cut(s) 139
BlpI GCTNAGC 1 cut(s) 30
BmiI GGNNCC 1 cut(s) 92
BmrI ACTGGG 1 cut(s) 208
BmsI GCATC 1 cut(s) 83
BmuI ACTGGG 1 cut(s) 208
Bpu1102I GCTNAGC 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 80
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 80
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 203
Bsh1236I CGCG 1 cut(s) 195
BshFI GGCC 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 155
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 125
BsnI GGCC 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 155
Bsp143I GATC 2 cut(s) 139, 187
Bsp1720I GCTNAGC 1 cut(s) 30
BspANI GGCC 1 cut(s) 148
BspCNI CTCAG 1 cut(s) 135
BspFNI CGCG 1 cut(s) 195
BspLI GGNNCC 1 cut(s) 92
BspPI GGATC 1 cut(s) 195
BsrI ACTGG 1 cut(s) 203
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 2 cut(s) 139, 187
BssT1I CCWWGG 1 cut(s) 80
Bst4CI ACNGT 2 cut(s) 116, 233
BstDEI CTNAG 2 cut(s) 30, 143
BstFNI CGCG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 2 cut(s) 142, 190
BstMBI GATC 2 cut(s) 139, 187
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstUI CGCG 1 cut(s) 195
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 1 cut(s) 148
BtgZI GCGATG 1 cut(s) 60
CfoI GCGC 1 cut(s) 195
CspCI CAANNNNNGTGG 2 cut(s) 237, 272
CviAII CATG 1 cut(s) 7
CviJI RGCY 5 cut(s) 11, 29, 148, 153, 167
CviKI_1 RGCY 5 cut(s) 11, 29, 148, 153, 167
DdeI CTNAG 2 cut(s) 30, 143
DpnI GATC 2 cut(s) 141, 189
DpnII GATC 2 cut(s) 139, 187
Ecl136II GAGCTC 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 80
Eco24I GRGCYC 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 80
EcoT38I GRGCYC 1 cut(s) 155
ErhI CCWWGG 1 cut(s) 80
FaeI CATG 1 cut(s) 10
FaiI YATR 2 cut(s) 8, 98
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 1 cut(s) 125
FatI CATG 1 cut(s) 6
FriOI GRGCYC 1 cut(s) 155
FspBI CTAG 1 cut(s) 18
GlaI GCGC 1 cut(s) 194
HaeIII GGCC 1 cut(s) 148
HhaI GCGC 1 cut(s) 195
Hin1II CATG 1 cut(s) 10
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
Hpy188I TCNGA 2 cut(s) 144, 181
Hpy99I CGWCG 1 cut(s) 233
HpyCH4III ACNGT 2 cut(s) 116, 233
HpyCH4V TGCA 1 cut(s) 246
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 2 cut(s) 30, 143
Hsp92II CATG 1 cut(s) 10
HspAI GCGC 1 cut(s) 193
Kzo9I GATC 2 cut(s) 139, 187
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 2 cut(s) 8, 184
LweI GCATC 1 cut(s) 83
MaeI CTAG 1 cut(s) 18
MalI GATC 2 cut(s) 141, 189
MboI GATC 2 cut(s) 139, 187
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 1 cut(s) 155
MluCI AATT 1 cut(s) 182
MnlI CCTC 2 cut(s) 127, 138
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 159
NdeII GATC 2 cut(s) 139, 187
NlaIII CATG 1 cut(s) 10
NlaIV GGNNCC 1 cut(s) 92
NmeAIII GCCGAG 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 92
PsuI RGATCY 1 cut(s) 139
SacI GAGCTC 1 cut(s) 155
Sau3AI GATC 2 cut(s) 139, 187
SduI GDGCHC 1 cut(s) 155
SetI ASST 1 cut(s) 155
SfaNI GCATC 1 cut(s) 83
SgeI CNNG 9 cut(s) 19, 30, 35, 80, 93, 162, 175, 206, 211
Sse9I AATT 1 cut(s) 182
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 1 cut(s) 155
StyI CCWWGG 1 cut(s) 80
TaaI ACNGT 2 cut(s) 116, 233
TasI AATT 1 cut(s) 182
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.