Rh1AG154100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001119
Physical Location & Seq
Forward (+)
28799287 .. 28811918
12632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG154100.1

Sequence Viewer

Length: 471 bp
ATGCAAAGGACAGATCAGTCGAGCCCAGAAGTTTCGAGGCTTCCAACCCGAGCCGGCGACCTGAGAATGTGGTCCCAACGTATGTTCGCCGTCCGGCCATCGCACTGGTTTTTTTTCTCTTCGTCCTGCCGTCGCGGTCATTCTATTGGCCTTGGTTTAATCGTTAGTCGACCTCAGAGAGAGAGAATCGAAGGTCAAATATTGAATAGGGAATTGAATTTGAATTCTTGCATCGCTAACGGTTGCCCCATTGAATCAGCTGTGTTTCGTACTGAAGTGCAGCATGAAGAAATTTTGCATTTGAGGGACATTCCCAGAACTTTGTTGTTCATCCACTTGGAGAAGTATCTTGTATGCTGCGTTGGAAGAGGCATGATGGATCCGGATATGCTTTTCGTTCCTGCTGGGAGGATTGACGGAGGTGCCATTGATAGCTTGGAGGTGGAGAGGGTTCCGGCTATCAGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

17.77

Weight (kDa)

8.4

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 422
AccI GTMKAC 1 cut(s) 169
AccII CGCG 1 cut(s) 135
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 1 cut(s) 135
AclWI GGATC 2 cut(s) 374, 387
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 3 cut(s) 217, 223, 291
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 271
AgsI TTSAA 4 cut(s) 205, 217, 223, 254
AluBI AGCT 2 cut(s) 260, 435
AluI AGCT 2 cut(s) 260, 435
AlwI GGATC 2 cut(s) 374, 387
Ama87I CYCGRG 1 cut(s) 48
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 3 cut(s) 95, 148, 465
ApeKI GCWGC 2 cut(s) 280, 357
ApoI RAATTY 3 cut(s) 217, 223, 291
AspS9I GGNCC 2 cut(s) 72, 465
AvaI CYCGRG 1 cut(s) 48
AvaII GGWCC 1 cut(s) 72
BamHI GGATCC 1 cut(s) 379
BanI GGYRCC 1 cut(s) 422
BanII GRGCYC 1 cut(s) 26
BbvI GCAGC 2 cut(s) 292, 344
BccI CCATC 2 cut(s) 106, 370
BceAI ACGGC 2 cut(s) 74, 114
BfaI CTAG 1 cut(s) 469
BisI GCNGC 2 cut(s) 281, 358
BlsI GCNGC 2 cut(s) 282, 359
Bme18I GGWCC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 48
BmgT120I GGNCC 2 cut(s) 72, 465
BmiI GGNNCC 4 cut(s) 74, 381, 424, 453
BmsI GCATC 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 151
BsaWI WCCGGW 1 cut(s) 382
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 110
BseAI TCCGGA 1 cut(s) 382
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 1 cut(s) 330
BseMII CTCAG 2 cut(s) 53, 188
BseNI ACTGG 1 cut(s) 110
BseXI GCAGC 2 cut(s) 292, 344
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 1 cut(s) 299
Bsh1236I CGCG 1 cut(s) 135
BshFI GGCC 3 cut(s) 97, 150, 467
BshNI GGYRCC 1 cut(s) 422
BsiHKCI CYCGRG 1 cut(s) 48
BsiSI CCGG 4 cut(s) 54, 94, 383, 455
BslFI GGGAC 2 cut(s) 58, 320
BsmFI GGGAC 2 cut(s) 58, 320
BsnI GGCC 3 cut(s) 97, 150, 467
BsoBI CYCGRG 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 26
Bsp13I TCCGGA 1 cut(s) 382
Bsp143I GATC 2 cut(s) 13, 379
BspACI CCGC 1 cut(s) 135
BspANI GGCC 3 cut(s) 97, 150, 467
BspCNI CTCAG 2 cut(s) 54, 187
BspEI TCCGGA 1 cut(s) 382
BspFNI CGCG 1 cut(s) 135
BspLI GGNNCC 4 cut(s) 74, 381, 424, 453
BspPI GGATC 2 cut(s) 374, 387
BspT107I GGYRCC 1 cut(s) 422
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 2 cut(s) 13, 379
BssT1I CCWWGG 1 cut(s) 151
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 2 cut(s) 124, 361
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 2 cut(s) 62, 174
BstF5I GGATG 1 cut(s) 330
BstFNI CGCG 1 cut(s) 135
BstKTI GATC 2 cut(s) 16, 382
BstMBI GATC 2 cut(s) 13, 379
BstMWI GCNNNNNNNGC 1 cut(s) 464
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 2 cut(s) 292, 344
BstX2I RGATCY 1 cut(s) 379
BstXI CCANNNNNNTGG 1 cut(s) 105
BstYI RGATCY 1 cut(s) 379
BsuRI GGCC 3 cut(s) 97, 150, 467
BtgZI GCGATG 2 cut(s) 84, 217
BtsCI GGATG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 103
Cac8I GCNNGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 72, 465
Csp6I GTAC 1 cut(s) 270
CviAII CATG 2 cut(s) 284, 373
CviJI RGCY 9 cut(s) 24, 40, 53, 97, 150, 260, 435, 458, 467
CviKI_1 RGCY 9 cut(s) 24, 40, 53, 97, 150, 260, 435, 458, 467
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 2 cut(s) 62, 174
DpnI GATC 2 cut(s) 15, 381
DpnII GATC 2 cut(s) 13, 379
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 2 cut(s) 124, 361
EarI CTCTTC 2 cut(s) 124, 361
Eco130I CCWWGG 1 cut(s) 151
Eco24I GRGCYC 1 cut(s) 26
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 48
EcoO109I RGGNCCY 1 cut(s) 465
EcoRI GAATTC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 151
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 2 cut(s) 287, 376
FaiI YATR 5 cut(s) 83, 285, 355, 374, 389
FaqI GGGAC 2 cut(s) 58, 320
FatI CATG 2 cut(s) 283, 372
FblI GTMKAC 1 cut(s) 169
Fnu4HI GCNGC 2 cut(s) 281, 358
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 2 cut(s) 281, 358
FspBI CTAG 1 cut(s) 469
GluI GCNGC 2 cut(s) 281, 358
GsaI CCCAGC 1 cut(s) 408
HaeIII GGCC 3 cut(s) 97, 150, 467
HapII CCGG 4 cut(s) 54, 94, 383, 455
Hin1II CATG 2 cut(s) 287, 376
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HinfI GANTC 2 cut(s) 186, 254
HpaII CCGG 4 cut(s) 54, 94, 383, 455
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 1 cut(s) 177
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 1 cut(s) 170
Hpy99I CGWCG 1 cut(s) 135
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 4 cut(s) 4, 231, 280, 298
HpyF10VI GCNNNNNNNGC 1 cut(s) 464
HpyF3I CTNAG 2 cut(s) 62, 174
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 2 cut(s) 287, 376
Kpn2I TCCGGA 1 cut(s) 382
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 2 cut(s) 13, 379
Lsp1109I GCAGC 2 cut(s) 292, 344
LweI GCATC 1 cut(s) 240
MaeI CTAG 1 cut(s) 469
MaeII ACGT 1 cut(s) 79
MalI GATC 2 cut(s) 15, 381
MboI GATC 2 cut(s) 13, 379
MboII GAAGA 3 cut(s) 111, 299, 378
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 26
MluCI AATT 4 cut(s) 212, 217, 223, 291
MmeI TCCRAC 2 cut(s) 68, 343
MnlI CCTC 8 cut(s) 30, 183, 297, 362, 402, 413, 433, 441
MroI TCCGGA 1 cut(s) 382
MroNI GCCGGC 1 cut(s) 53
MseI TTAA 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 4 cut(s) 54, 94, 383, 455
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 1 cut(s) 464
NaeI GCCGGC 1 cut(s) 55
NdeII GATC 2 cut(s) 13, 379
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 2 cut(s) 287, 376
NlaIV GGNNCC 4 cut(s) 74, 381, 424, 453
PdiI GCCGGC 1 cut(s) 55
PfeI GAWTC 2 cut(s) 186, 254
PkrI GCNGC 2 cut(s) 282, 359
PspFI CCCAGC 1 cut(s) 404
PspN4I GGNNCC 4 cut(s) 74, 381, 424, 453
PspPI GGNCC 2 cut(s) 72, 465
PsuI RGATCY 1 cut(s) 379
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
SalI GTCGAC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 158
SatI GCNGC 2 cut(s) 281, 358
Sau3AI GATC 2 cut(s) 13, 379
Sau96I GGNCC 2 cut(s) 72, 465
SduI GDGCHC 1 cut(s) 26
SetI ASST 8 cut(s) 63, 82, 175, 196, 262, 424, 437, 444
SfaNI GCATC 1 cut(s) 240
SinI GGWCC 1 cut(s) 72
Sse9I AATT 4 cut(s) 212, 217, 223, 291
SsiI CCGC 1 cut(s) 135
SspI AATATT 1 cut(s) 201
SspMI CTAG 1 cut(s) 469
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 1 cut(s) 82
TaqI TCGA 4 cut(s) 20, 35, 169, 189
TasI AATT 4 cut(s) 212, 217, 223, 291
TfiI GAWTC 2 cut(s) 186, 254
Tru1I TTAA 1 cut(s) 158
Tru9I TTAA 1 cut(s) 158
TscAI CASTG 1 cut(s) 110
TseI GCWGC 2 cut(s) 280, 357
TspDTI ATGAA 2 cut(s) 300, 319
TspGWI ACGGA 1 cut(s) 432
TspRI CASTG 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 3 cut(s) 217, 223, 291
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 169
XspI CTAG 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.