Rh2DG378200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001119
Physical Location & Seq
Forward (+)
54291654 .. 54292070
417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG378200.1

Sequence Viewer

Length: 417 bp
ATGGCCATGGCCATTGCTACCCTTGTGGCTTATTGTTTCTTGCTTCTACTTGCTTTTGTACTGTTGCTTGGAGCGCCGCCTTGGATTTGGTTCCCAGCGGTTGTTGTCTGGGACGGCAGTTATGGTTTTCTGGTAGCTTCTCTAAGGCCGAGCTCTAACAACGTGGATCTGAATTGGTCTGTATCGAATCGATACGACTGGGTCTTGGATCAGAGTCGTCCGGCTGGCCTATTTGCGGTGGTTGGGTGTCGTCGTAGGGCGGACATGGTCGTTCTTGATTTGGATTATAGCTGTTCGGGTTTCAAGCCAAATTTTGTTGGTTCTGGTTTGAAGGACATGGTGGATGATCGCAATGTTCGCACTGATGTTAGACATGGTGGTCGCTGCAATGTCGAAGAGTTGCCAGAGTTAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

15.31

Weight (kDa)

5.22

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 378
AciI CCGC 4 cut(s) 77, 98, 236, 260
AclWI GGATC 2 cut(s) 174, 216
AcoI YGGCCR 2 cut(s) 3, 9
AcsI RAATTY 1 cut(s) 310
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 2 cut(s) 235, 410
AgsI TTSAA 2 cut(s) 304, 331
AluBI AGCT 3 cut(s) 137, 153, 291
AluI AGCT 3 cut(s) 137, 153, 291
Alw21I GWGCWC 1 cut(s) 155
AlwI GGATC 2 cut(s) 174, 216
AoxI GGCC 4 cut(s) 3, 9, 146, 226
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 1 cut(s) 310
AspLEI GCGC 1 cut(s) 76
BalI TGGCCA 2 cut(s) 5, 11
BanII GRGCYC 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 371
BceAI ACGGC 1 cut(s) 130
BfoI RGCGCY 1 cut(s) 77
BisI GCNGC 2 cut(s) 77, 385
BlsI GCNGC 2 cut(s) 78, 386
BmiI GGNNCC 1 cut(s) 92
BmrI ACTGGG 1 cut(s) 208
BmuI ACTGGG 1 cut(s) 208
Bsa29I ATCGAT 1 cut(s) 190
BsaJI CCNNGG 2 cut(s) 6, 80
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 410
Bse1I ACTGG 1 cut(s) 203
Bse3DI GCAATG 3 cut(s) 12, 358, 394
BseCI ATCGAT 1 cut(s) 190
BseDI CCNNGG 2 cut(s) 6, 80
BseGI GGATG 1 cut(s) 349
BseLI CCNNNNNNNGG 2 cut(s) 235, 410
BseMI GCAATG 3 cut(s) 12, 358, 394
BseNI ACTGG 1 cut(s) 203
BseXI GCAGC 1 cut(s) 371
BseYI CCCAGC 1 cut(s) 94
BshFI GGCC 4 cut(s) 5, 11, 148, 228
BshVI ATCGAT 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 155
BsiSI CCGG 1 cut(s) 221
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 2 cut(s) 235, 410
BsmFI GGGAC 1 cut(s) 125
BsnI GGCC 4 cut(s) 5, 11, 148, 228
Bsp1286I GDGCHC 1 cut(s) 155
Bsp143I GATC 3 cut(s) 166, 208, 346
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 4 cut(s) 77, 98, 236, 260
BspANI GGCC 4 cut(s) 5, 11, 148, 228
BspDI ATCGAT 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 92
BspPI GGATC 2 cut(s) 174, 216
BsrDI GCAATG 3 cut(s) 12, 358, 394
BsrI ACTGG 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 6, 80
BssMI GATC 3 cut(s) 166, 208, 346
BssT1I CCWWGG 2 cut(s) 6, 80
Bst4CI ACNGT 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 390
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 1 cut(s) 143
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 349
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 3 cut(s) 169, 211, 349
BstMBI GATC 3 cut(s) 166, 208, 346
BstMWI GCNNNNNNNGC 2 cut(s) 73, 357
BstV1I GCAGC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
Bsu15I ATCGAT 1 cut(s) 190
BsuRI GGCC 4 cut(s) 5, 11, 148, 228
BsuTUI ATCGAT 1 cut(s) 190
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 226
CfoI GCGC 1 cut(s) 76
ClaI ATCGAT 1 cut(s) 190
Csp6I GTAC 1 cut(s) 59
CviAII CATG 4 cut(s) 7, 265, 337, 374
CviQI GTAC 1 cut(s) 59
DdeI CTNAG 1 cut(s) 143
DpnI GATC 3 cut(s) 168, 210, 348
DpnII GATC 3 cut(s) 166, 208, 346
DrdI GACNNNNNNGTC 1 cut(s) 378
DseDI GACNNNNNNGTC 1 cut(s) 378
EaeI YGGCCR 2 cut(s) 3, 9
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
EciI GGCGGA 1 cut(s) 275
Ecl136II GAGCTC 1 cut(s) 153
Eco130I CCWWGG 2 cut(s) 6, 80
Eco24I GRGCYC 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 153
EcoT14I CCWWGG 2 cut(s) 6, 80
EcoT38I GRGCYC 1 cut(s) 155
ErhI CCWWGG 2 cut(s) 6, 80
FaeI CATG 4 cut(s) 10, 268, 340, 377
FaiI YATR 6 cut(s) 8, 123, 266, 288, 338, 375
FaqI GGGAC 1 cut(s) 125
FatI CATG 4 cut(s) 6, 264, 336, 373
Fnu4HI GCNGC 2 cut(s) 77, 385
FokI GGATG 1 cut(s) 356
FriOI GRGCYC 1 cut(s) 155
Fsp4HI GCNGC 2 cut(s) 77, 385
GlaI GCGC 1 cut(s) 75
GluI GCNGC 2 cut(s) 77, 385
GsaI CCCAGC 1 cut(s) 98
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 4 cut(s) 5, 11, 148, 228
HapII CCGG 1 cut(s) 221
HhaI GCGC 1 cut(s) 76
Hin1II CATG 4 cut(s) 10, 268, 340, 377
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 2 cut(s) 187, 214
HpaII CCGG 1 cut(s) 221
Hpy188I TCNGA 2 cut(s) 171, 213
Hpy188III TCNNGA 1 cut(s) 275
Hpy99I CGWCG 1 cut(s) 255
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 1 cut(s) 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 357
HpyF3I CTNAG 1 cut(s) 143
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 4 cut(s) 10, 268, 340, 377
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 3 cut(s) 166, 208, 346
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 7 cut(s) 94, 108, 116, 184, 210, 234, 309
Lsp1109I GCAGC 1 cut(s) 371
MaeII ACGT 1 cut(s) 162
MalI GATC 3 cut(s) 168, 210, 348
MboI GATC 3 cut(s) 166, 208, 346
MboII GAAGA 1 cut(s) 407
MflI RGATCY 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 155
MlsI TGGCCA 2 cut(s) 5, 11
MluCI AATT 2 cut(s) 172, 310
MluNI TGGCCA 2 cut(s) 5, 11
MlyI GAGTC 1 cut(s) 223
Mox20I TGGCCA 2 cut(s) 5, 11
MscI TGGCCA 2 cut(s) 5, 11
Msp20I TGGCCA 2 cut(s) 5, 11
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 73, 357
NcoI CCATGG 1 cut(s) 6
NdeII GATC 3 cut(s) 166, 208, 346
NlaIII CATG 4 cut(s) 10, 268, 340, 377
NlaIV GGNNCC 1 cut(s) 92
NmeAIII GCCGAG 1 cut(s) 174
PfeI GAWTC 1 cut(s) 187
PflFI GACNNNGTC 2 cut(s) 200, 266
PkrI GCNGC 2 cut(s) 78, 386
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 155
PspFI CCCAGC 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 92
PsuI RGATCY 1 cut(s) 166
PsyI GACNNNGTC 2 cut(s) 200, 266
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
SacI GAGCTC 1 cut(s) 155
SatI GCNGC 2 cut(s) 77, 385
Sau3AI GATC 3 cut(s) 166, 208, 346
SchI GAGTC 1 cut(s) 223
SduI GDGCHC 1 cut(s) 155
SetI ASST 5 cut(s) 139, 155, 165, 293, 415
Sse9I AATT 2 cut(s) 172, 310
SsiI CCGC 4 cut(s) 77, 98, 236, 260
SstI GAGCTC 1 cut(s) 155
StyI CCWWGG 2 cut(s) 6, 80
TaaI ACNGT 1 cut(s) 63
TaiI ACGT 1 cut(s) 165
TaqI TCGA 3 cut(s) 185, 190, 393
TasI AATT 2 cut(s) 172, 310
TatI WGTACW 1 cut(s) 58
TauI GCSGC 1 cut(s) 79
TfiI GAWTC 1 cut(s) 187
TscAI CASTG 1 cut(s) 367
TseI GCWGC 1 cut(s) 384
TspRI CASTG 1 cut(s) 367
Tth111I GACNNNGTC 2 cut(s) 200, 266
XapI RAATTY 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.