RLG00000036453

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001119
Physical Location & Seq
Forward (+)
80805073 .. 80805725
653 bp
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UTR
Exon/CDS
Intron
RLM00000036453

Sequence Viewer

Length: 375 bp
ATGGCCATTGCTAGCCTGGTGGCTAATCGTTTCTTTCCTCTTGTTGCTTTTGTTCTATTTCTTGGAGTGCCGTCTTGGATTGGGTTCCCTGTGGTTGCTGTCTGGGACAGCGGTTTTGATGTTTTGGTGGCTGATCTGAGGCCGAGCTCTAACGGCGTGGATCTGGTTTGGTCTGCATTGGATCTGCGAGACTGGATTTTGGATCGTGGTAGTCTGACTACCGACAGTGGTGGGGGCTACACAAACGCCGTTGATGGTGTTTGGGAACAAAAGGGGGACGCCGACGGAGGTGAGGTTGCTGTCAGCCCGGGATACAGCAAGCTGCTCGGAGGTGACGATGCAATGGAGGATGCTGCTAGGGTTTTTCCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.12

Weight (kDa)

4.05

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 111
AclWI GGATC 3 cut(s) 168, 189, 210
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 279
AjnI CCWGG 1 cut(s) 15
AluBI AGCT 2 cut(s) 147, 322
AluI AGCT 2 cut(s) 147, 322
Alw21I GWGCWC 1 cut(s) 149
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 3 cut(s) 168, 189, 210
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 2 cut(s) 3, 140
ApeKI GCWGC 2 cut(s) 322, 353
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
AsuC2I CCSGG 2 cut(s) 308, 309
AsuHPI GGTGA 2 cut(s) 302, 344
AsuNHI GCTAGC 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 307
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 149
BbvI GCAGC 2 cut(s) 309, 340
BccI CCATC 1 cut(s) 248
BceAI ACGGC 3 cut(s) 55, 169, 233
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 1 cut(s) 17
BciVI GTATCC 1 cut(s) 305
BcnI CCSGG 2 cut(s) 308, 309
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 3 cut(s) 12, 357, 373
BfuI GTATCC 1 cut(s) 305
BisI GCNGC 2 cut(s) 323, 354
BlsI GCNGC 2 cut(s) 324, 355
Bme1390I CCNGG 3 cut(s) 17, 308, 309
BmeT110I CYCGRG 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 86
BmrFI CCNGG 3 cut(s) 17, 308, 309
BmsI GCATC 2 cut(s) 328, 340
BmtI GCTAGC 1 cut(s) 15
BpuMI CCSGG 2 cut(s) 308, 309
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 1 cut(s) 307
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bse1I ACTGG 1 cut(s) 197
Bse3DI GCAATG 2 cut(s) 6, 348
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 307
BseGI GGATG 1 cut(s) 355
BseMI GCAATG 2 cut(s) 6, 348
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 197
BseXI GCAGC 2 cut(s) 309, 340
BshFI GGCC 2 cut(s) 5, 142
BsiHKAI GWGCWC 1 cut(s) 149
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 1 cut(s) 308
BslFI GGGAC 2 cut(s) 119, 290
BsmAI GTCTC 1 cut(s) 183
BsmFI GGGAC 2 cut(s) 119, 290
BsnI GGCC 2 cut(s) 5, 142
BsoBI CYCGRG 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 4 cut(s) 133, 160, 181, 202
BspACI CCGC 1 cut(s) 111
BspANI GGCC 2 cut(s) 5, 142
BspCNI CTCAG 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 86
BspOI GCTAGC 1 cut(s) 15
BspPI GGATC 3 cut(s) 168, 189, 210
BsrDI GCAATG 2 cut(s) 6, 348
BsrI ACTGG 1 cut(s) 197
BssECI CCNNGG 1 cut(s) 307
BssMI GATC 4 cut(s) 133, 160, 181, 202
BssNI GRCGYC 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 1 cut(s) 227
BstACI GRCGYC 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 13, 320
BstDEI CTNAG 1 cut(s) 137
BstF5I GGATG 1 cut(s) 355
BstKTI GATC 4 cut(s) 136, 163, 184, 205
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 4 cut(s) 133, 160, 181, 202
BstMWI GCNNNNNNNGC 1 cut(s) 153
BstNI CCWGG 1 cut(s) 17
BstSCI CCNGG 3 cut(s) 15, 306, 307
BstV1I GCAGC 2 cut(s) 309, 340
BstX2I RGATCY 2 cut(s) 160, 181
BstYI RGATCY 2 cut(s) 160, 181
BsuI GTATCC 1 cut(s) 305
BsuRI GGCC 2 cut(s) 5, 142
BtsCI GGATG 1 cut(s) 355
BtsIMutI CAGTG 1 cut(s) 232
Cac8I GCNNGC 2 cut(s) 13, 320
Cfr9I CCCGGG 1 cut(s) 307
CseI GACGC 1 cut(s) 287
CviJI RGCY 9 cut(s) 5, 15, 23, 131, 142, 147, 237, 306, 322
CviKI_1 RGCY 9 cut(s) 5, 15, 23, 131, 142, 147, 237, 306, 322
DdeI CTNAG 1 cut(s) 137
DpnI GATC 4 cut(s) 135, 162, 183, 204
DpnII GATC 4 cut(s) 133, 160, 181, 202
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 147
Eco88I CYCGRG 1 cut(s) 307
EcoICRI GAGCTC 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 149
FaqI GGGAC 2 cut(s) 119, 290
Fnu4HI GCNGC 2 cut(s) 323, 354
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 2 cut(s) 323, 354
FspBI CTAG 3 cut(s) 12, 357, 373
GluI GCNGC 2 cut(s) 323, 354
HaeIII GGCC 2 cut(s) 5, 142
HapII CCGG 1 cut(s) 308
HgaI GACGC 1 cut(s) 287
Hin1I GRCGYC 1 cut(s) 279
HpaII CCGG 1 cut(s) 308
HphI GGTGA 2 cut(s) 302, 344
Hpy188I TCNGA 3 cut(s) 138, 216, 329
Hpy99I CGWCG 1 cut(s) 287
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4V TGCA 2 cut(s) 176, 341
HpyF10VI GCNNNNNNNGC 1 cut(s) 153
HpyF3I CTNAG 1 cut(s) 137
Hsp92I GRCGYC 1 cut(s) 279
Kzo9I GATC 4 cut(s) 133, 160, 181, 202
LpnPI CCDG 7 cut(s) 2, 29, 88, 102, 149, 178, 321
Lsp1109I GCAGC 2 cut(s) 309, 340
LweI GCATC 2 cut(s) 328, 340
MaeI CTAG 3 cut(s) 12, 357, 373
MaeIII GTNAC 1 cut(s) 332
MalI GATC 4 cut(s) 135, 162, 183, 204
MboI GATC 4 cut(s) 133, 160, 181, 202
MflI RGATCY 2 cut(s) 160, 181
MhlI GDGCHC 1 cut(s) 149
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 6 cut(s) 48, 132, 281, 286, 323, 340
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 3 cut(s) 17, 308, 309
MvaI CCWGG 1 cut(s) 17
MwoI GCNNNNNNNGC 1 cut(s) 153
NciI CCSGG 2 cut(s) 308, 309
NdeII GATC 4 cut(s) 133, 160, 181, 202
NheI GCTAGC 1 cut(s) 11
NlaIV GGNNCC 1 cut(s) 86
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 332
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PkrI GCNGC 2 cut(s) 324, 355
Psp124BI GAGCTC 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 15
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 86
PsuI RGATCY 2 cut(s) 160, 181
SacI GAGCTC 1 cut(s) 149
SatI GCNGC 2 cut(s) 323, 354
Sau3AI GATC 4 cut(s) 133, 160, 181, 202
ScrFI CCNGG 3 cut(s) 17, 308, 309
SduI GDGCHC 1 cut(s) 149
SetI ASST 5 cut(s) 149, 292, 297, 324, 334
SfaNI GCATC 2 cut(s) 328, 340
SmaI CCCGGG 1 cut(s) 309
SsiI CCGC 1 cut(s) 111
SspMI CTAG 3 cut(s) 12, 357, 373
SstI GAGCTC 1 cut(s) 149
StyD4I CCNGG 3 cut(s) 15, 306, 307
TaaI ACNGT 1 cut(s) 227
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 322, 353
Tsp45I GTSAC 1 cut(s) 332
TspGWI ACGGA 1 cut(s) 300
TspMI CCCGGG 1 cut(s) 307
TspRI CASTG 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XmaI CCCGGG 1 cut(s) 307
XspI CTAG 3 cut(s) 12, 357, 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.