Rmu_sc0000161.1_g000054

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000161.1
OG0000976
Physical Location & Seq
Forward (+)
222798 .. 223926
1129 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
atgaaaaatatactgaaggacaagaaagtgcctacagtaaaaaagaaagctaatggattcaaaccattagaatggccagacaagaatcctattctaaacatgatttttctggatccttcaactagttctactagtatccggtatgaaaatccgaaagaaactcgagatgtcaaaatgataagcatcttcgggaagaagaaaggagttcaactcctaaatgctgaagaattcgaatacaatgaaatcgagcatgagctgggaactctagaagcatctctgtgcaatcaagtggcatatttcaactgcttccccaacttctcaaccagcttgaaggatgcagcccactgtctccgactgagagtcaagacagatggcatatcaatgaaaaatgatatgcaagaattggcgatagtatacagaatatactataaactgatgagtaccacagtagagccaaagacacggatctccaatatccctggtcttactactggattcctcaccagccagaagaaccattcacaacagattcacaaggttacttggaatgaagtaatttttcctattgaatggaaattatctggtccgaagctaccagcagagagtgcaaaagcaaaaatttatgaaagtagaaagactggagaaatcagtcaaaattttgacaatcacagaaaaagtgatgtcggtcctgagaatctcaggataaacaggaatcttcttagaagaagctacagcactagaaaagctagtgtcagtggtacaaaacccactattgaagatcctattactgaagaagaactagaagctgatctaaacagaccagtctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

275

Amino Acids

31.48

Weight (kDa)

9.29

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MP PF01107 84 - 149 1.8e-06 Viral movement protein (MP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000976)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24112 FvH4_4g10003
rosa_chinensis RchiOBHm_Chr3g0487531 RchiOBHm_Chr5g0034781 RchiOBHm_Chr6g0277631
rosa_laevigata RLG00000021184
rosa_multiflora Rmu_sc0000144.1_g000006 Rmu_sc0000161.1_g000054 Rmu_sc0000383.1_g000008 Rmu_sc0000420.1_g000038 Rmu_sc0000429.1_g000090 Rmu_sc0000429.1_g000091 Rmu_sc0000453.1_g000018 Rmu_sc0000458.1_g000020 Rmu_sc0000483.1_g000003 Rmu_sc0000531.1_g000007 Rmu_sc0000595.1_g000055 Rmu_sc0000768.1_g000022 Rmu_sc0000855.1_g000012 Rmu_sc0000974.1_g000020 Rmu_sc0001018.1_g000020 Rmu_sc0001265.1_g000003 Rmu_sc0001407.1_g000004 Rmu_sc0001694.1_g000007 Rmu_sc0001785.1_g000004 Rmu_sc0001847.1_g000011 Rmu_sc0002148.1_g000003 Rmu_sc0002316.1_g000089 Rmu_sc0002501.1_g000027 Rmu_sc0002521.1_g000031 Rmu_sc0002845.1_g000028 Rmu_sc0003315.1_g000022 Rmu_sc0003371.1_g000015 Rmu_sc0003386.1_g000006 Rmu_sc0003415.1_g000012 Rmu_sc0003467.1_g000010 Rmu_sc0003583.1_g000002 Rmu_sc0003832.1_g000032 Rmu_sc0004094.1_g000040 Rmu_sc0004286.1_g000014 Rmu_sc0004344.1_g000014 Rmu_sc0004798.1_g000005 Rmu_sc0004846.1_g000001 Rmu_sc0005362.1_g000001 Rmu_sc0005481.1_g000002 Rmu_sc0006498.1_g000005 Rmu_sc0006968.1_g000011 Rmu_sc0007369.1_g000004 Rmu_sc0007727.1_g000016 Rmu_sc0008681.1_g000002 Rmu_sc0010278.1_g000009 Rmu_sc0011015.1_g000002 Rmu_sc0011611.1_g000004 Rmu_sc0012216.1_g000005 Rmu_sc0013333.1_g000001 Rmu_sc0016916.1_g000001 Rmu_sc0024220.1_g000001 Rmu_sc0036248.1_g000001 Rmu_sc0037776.1_g000001 Rmu_ssc0000052.1_g000014
rosa_samantha Rh4CG090000
rosa_wichuraiana Rw2G035730 Rw5G039910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 414
AclWI GGATC 4 cut(s) 107, 120, 473, 773
AcoI YGGCCR 1 cut(s) 74
AcsI RAATTY 3 cut(s) 227, 618, 655
AcuI CTGAAG 3 cut(s) 35, 243, 810
AfaI GTAC 2 cut(s) 442, 760
AgsI TTSAA 7 cut(s) 61, 120, 209, 301, 331, 569, 776
AhdI GACNNNNNGTC 1 cut(s) 359
AhlI ACTAGT 2 cut(s) 122, 131
AjnI CCWGG 1 cut(s) 478
AluBI AGCT 7 cut(s) 50, 256, 327, 592, 729, 746, 806
AluI AGCT 7 cut(s) 50, 256, 327, 592, 729, 746, 806
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 4 cut(s) 107, 120, 473, 773
Ama87I CYCGRG 1 cut(s) 162
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 3 cut(s) 227, 618, 655
AspS9I GGNCC 2 cut(s) 584, 686
AsuHPI GGTGA 1 cut(s) 493
AsuII TTCGAA 1 cut(s) 231
AvaI CYCGRG 1 cut(s) 162
AvaII GGWCC 2 cut(s) 584, 686
BalI TGGCCA 1 cut(s) 76
BamHI GGATCC 1 cut(s) 112
BbvI GCAGC 1 cut(s) 350
BccI CCATC 1 cut(s) 365
BciT130I CCWGG 1 cut(s) 480
BciVI GTATCC 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 353
BcuI ACTAGT 2 cut(s) 122, 131
BfaI CTAG 7 cut(s) 123, 132, 266, 738, 747, 800, 826
BfmI CTRYAG 2 cut(s) 33, 730
BfuI GTATCC 1 cut(s) 146
BisI GCNGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 340
Bme1390I CCNGG 1 cut(s) 480
Bme18I GGWCC 2 cut(s) 584, 686
BmeRI GACNNNNNGTC 1 cut(s) 359
BmeT110I CYCGRG 1 cut(s) 162
BmgT120I GGNCC 2 cut(s) 584, 686
BmiI GGNNCC 1 cut(s) 114
BmrFI CCNGG 1 cut(s) 480
BmsI GCATC 3 cut(s) 192, 281, 325
BplI GAGNNNNNCTC 2 cut(s) 195, 227
BpmI CTGGAG 1 cut(s) 660
Bpu14I TTCGAA 1 cut(s) 231
BsaBI GATNNNNATC 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 478
BsaWI WCCGGW 1 cut(s) 138
Bse1I ACTGG 3 cut(s) 496, 643, 821
Bse8I GATNNNNATC 1 cut(s) 182
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 1 cut(s) 340
BseJI GATNNNNATC 1 cut(s) 182
BseMII CTCAG 3 cut(s) 347, 681, 712
BseNI ACTGG 3 cut(s) 496, 643, 821
BseXI GCAGC 1 cut(s) 350
BseYI CCCAGC 1 cut(s) 256
BshFI GGCC 1 cut(s) 76
BsiHKCI CYCGRG 1 cut(s) 162
BsiSI CCGG 1 cut(s) 139
BsmAI GTCTC 1 cut(s) 353
BsnI GGCC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 162
Bsp119I TTCGAA 1 cut(s) 231
Bsp143I GATC 4 cut(s) 112, 465, 778, 808
BspANI GGCC 1 cut(s) 76
BspCNI CTCAG 3 cut(s) 348, 682, 711
BspLI GGNNCC 1 cut(s) 114
BspPI GGATC 4 cut(s) 107, 120, 473, 773
BspT104I TTCGAA 1 cut(s) 231
BsrI ACTGG 3 cut(s) 496, 643, 821
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 4 cut(s) 112, 465, 778, 808
BssNAI GTATAC 1 cut(s) 415
Bst1107I GTATAC 1 cut(s) 415
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 3 cut(s) 37, 347, 448
BstAPI GCANNNNNTGC 1 cut(s) 605
BstBI TTCGAA 1 cut(s) 231
BstDEI CTNAG 4 cut(s) 356, 690, 698, 719
BstF5I GGATG 1 cut(s) 340
BstKTI GATC 4 cut(s) 115, 468, 781, 811
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 4 cut(s) 112, 465, 778, 808
BstMWI GCNNNNNNNGC 1 cut(s) 605
BstNI CCWGG 1 cut(s) 480
BstSCI CCNGG 1 cut(s) 478
BstSFI CTRYAG 2 cut(s) 33, 730
BstV1I GCAGC 1 cut(s) 350
BstX2I RGATCY 3 cut(s) 112, 465, 778
BstXI CCANNNNNNTGG 1 cut(s) 72
BstYI RGATCY 3 cut(s) 112, 465, 778
BstZ17I GTATAC 1 cut(s) 415
BsuI GTATCC 1 cut(s) 146
BsuRI GGCC 1 cut(s) 76
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 2 cut(s) 343, 760
Cfr13I GGNCC 2 cut(s) 584, 686
Csp6I GTAC 2 cut(s) 441, 759
CviAII CATG 2 cut(s) 100, 251
CviQI GTAC 2 cut(s) 441, 759
DdeI CTNAG 4 cut(s) 356, 690, 698, 719
DpnI GATC 4 cut(s) 114, 467, 780, 810
DpnII GATC 4 cut(s) 112, 465, 778, 808
DriI GACNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 1 cut(s) 74
Eam1105I GACNNNNNGTC 1 cut(s) 359
Eco47I GGWCC 2 cut(s) 584, 686
Eco57I CTGAAG 3 cut(s) 35, 243, 810
Eco88I CYCGRG 1 cut(s) 162
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 478
FaeI CATG 2 cut(s) 103, 254
FatI CATG 2 cut(s) 99, 250
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 1 cut(s) 339
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 339
FspBI CTAG 7 cut(s) 123, 132, 266, 738, 747, 800, 826
GluI GCNGC 1 cut(s) 339
GsaI CCCAGC 1 cut(s) 260
GsuI CTGGAG 1 cut(s) 660
HaeIII GGCC 1 cut(s) 76
HapII CCGG 1 cut(s) 139
Hin1II CATG 2 cut(s) 103, 254
HinfI GANTC 7 cut(s) 57, 85, 360, 495, 529, 694, 712
HpaII CCGG 1 cut(s) 139
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 3 cut(s) 153, 353, 588
Hpy188III TCNNGA 7 cut(s) 110, 164, 190, 266, 364, 689, 700
Hpy8I GTNNAC 1 cut(s) 415
HpyAV CCTTC 3 cut(s) 10, 126, 325
HpyCH4III ACNGT 3 cut(s) 37, 347, 448
HpyCH4V TGCA 4 cut(s) 282, 338, 397, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 605
HpyF3I CTNAG 4 cut(s) 356, 690, 698, 719
Hsp92II CATG 2 cut(s) 103, 254
Kzo9I GATC 4 cut(s) 112, 465, 778, 808
Lsp1109I GCAGC 1 cut(s) 350
LweI GCATC 3 cut(s) 192, 281, 325
MaeI CTAG 7 cut(s) 123, 132, 266, 738, 747, 800, 826
MaeIII GTNAC 1 cut(s) 538
MalI GATC 4 cut(s) 114, 467, 780, 810
MboI GATC 4 cut(s) 112, 465, 778, 808
MflI RGATCY 3 cut(s) 112, 465, 778
MlsI TGGCCA 1 cut(s) 76
MluCI AATT 6 cut(s) 227, 401, 555, 575, 618, 655
MluNI TGGCCA 1 cut(s) 76
MlyI GAGTC 1 cut(s) 369
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 1 cut(s) 509
Mox20I TGGCCA 1 cut(s) 76
MscI TGGCCA 1 cut(s) 76
MslI CAYNNNNRTG 3 cut(s) 70, 277, 380
Msp20I TGGCCA 1 cut(s) 76
MspI CCGG 1 cut(s) 139
MspR9I CCNGG 1 cut(s) 480
MvaI CCWGG 1 cut(s) 480
MwoI GCNNNNNNNGC 1 cut(s) 605
NdeII GATC 4 cut(s) 112, 465, 778, 808
NlaIII CATG 2 cut(s) 103, 254
NlaIV GGNNCC 1 cut(s) 114
NspV TTCGAA 1 cut(s) 231
PaeR7I CTCGAG 1 cut(s) 162
PfeI GAWTC 6 cut(s) 57, 85, 495, 529, 694, 712
PkrI GCNGC 1 cut(s) 340
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 478
PspFI CCCAGC 1 cut(s) 256
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 114
PspPI GGNCC 2 cut(s) 584, 686
PsuI RGATCY 3 cut(s) 112, 465, 778
RsaI GTAC 2 cut(s) 442, 760
RsaNI GTAC 2 cut(s) 441, 759
RseI CAYNNNNRTG 3 cut(s) 70, 277, 380
SatI GCNGC 1 cut(s) 339
Sau3AI GATC 4 cut(s) 112, 465, 778, 808
Sau96I GGNCC 2 cut(s) 584, 686
SchI GAGTC 1 cut(s) 369
ScrFI CCNGG 1 cut(s) 480
SetI ASST 8 cut(s) 52, 258, 329, 540, 594, 731, 748, 808
SfaNI GCATC 3 cut(s) 192, 281, 325
SfcI CTRYAG 2 cut(s) 33, 730
Sfr274I CTCGAG 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 2 cut(s) 584, 686
SlaI CTCGAG 1 cut(s) 162
SmiMI CAYNNNNRTG 3 cut(s) 70, 277, 380
SmlI CTYRAG 1 cut(s) 162
SmoI CTYRAG 1 cut(s) 162
SpeI ACTAGT 2 cut(s) 122, 131
Sse9I AATT 6 cut(s) 227, 401, 555, 575, 618, 655
SspMI CTAG 7 cut(s) 123, 132, 266, 738, 747, 800, 826
StyD4I CCNGG 1 cut(s) 478
TaaI ACNGT 3 cut(s) 37, 347, 448
TaqI TCGA 3 cut(s) 163, 231, 246
TaqII GACCGA 1 cut(s) 674
TasI AATT 6 cut(s) 227, 401, 555, 575, 618, 655
TfiI GAWTC 6 cut(s) 57, 85, 495, 529, 694, 712
TscAI CASTG 2 cut(s) 350, 760
TseI GCWGC 1 cut(s) 338
TspDTI ATGAA 6 cut(s) 17, 159, 255, 398, 564, 639
TspGWI ACGGA 1 cut(s) 478
TspRI CASTG 2 cut(s) 350, 760
VpaK11BI GGWCC 2 cut(s) 584, 686
XapI RAATTY 3 cut(s) 227, 618, 655
XbaI TCTAGA 1 cut(s) 265
XhoI CTCGAG 1 cut(s) 162
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 7 cut(s) 123, 132, 266, 738, 747, 800, 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.