AT1G13060

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000613
Physical Location & Seq
Forward (+)
4452190 .. 4454900
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G13060.3

Sequence Viewer

Length: 825 bp
ATGAAGCTTGATACTAGTGGGTTCGAGACTTCCATGCCTATGATTGGATTTGGCTCGAGCAGTGATATGCTTGATGAGCTTTCTTCTGTACCCTCGTTTGATCTACCCCGTACTAAAGAGTTTGATGGATTTCAGAAAAAAGCTAAAGACATGTTGAAGCATGCAAAAGGAACAACCACTCTCGCTTTTATCTTCAAAGGTGGTGTTATGGTCGCTGCTGATTCTCGGGCTAGCATGGGAGGATATATCTCCTCACAATCTGTGAAGAAGATTATTGAAATCAATCCTTATATGCTCGGTACAATGGCTGGAGGAGCTGCTGATTGCCAATTCTGGCACAGAAATCTTGGAATTAAGTGCCGTCTACATGAGCTGGCAAACAAGAGGAGAATCTCTGTTTCCGGAGCTTCGAAACTTCTTGCCAACATGCTCTATTCATACCGTGGAATGGGACTTTCTGTTGGTACAATGATTGCTGGATGGGACGAAACTGGTCCCGGACTATACTATGTCGACAACGAAGGAGGACGGCTCAAGGGAGACAGGTTTTCAGTCGGTTCTGGTTCACCATATGCTTACGGTGTGCTGGACAGCGGGTACAAATATGATATGTCAGTTGAAGAAGCTTCCGAGTTAGCAAGGAGATCAATCTACCATGCGACATTCCGTGATGGAGCCAGTGGTGGAGTTGCTAGCGTGTACCACGTTGGTCCCGAAGGATGGACGAAACTATCAGGAGATGATGTTGGGGAGCTACACTACCATTACTACCCCGTGGCACCAGCTACCGCAGAACAAGTCATGGAGGAAGCAACAGCCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

274

Amino Acids

29.67

Weight (kDa)

6.0

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 108 - 260 2.3e-40 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 778
AccI GTMKAC 2 cut(s) 364, 513
AccIII TCCGGA 1 cut(s) 401
AciI CCGC 2 cut(s) 594, 789
AfaI GTAC 6 cut(s) 90, 112, 301, 466, 599, 701
AfiI CCNNNNNNNGG 3 cut(s) 44, 448, 720
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 157, 196, 278, 620
AhlI ACTAGT 1 cut(s) 14
AluBI AGCT 9 cut(s) 7, 79, 143, 317, 373, 407, 626, 754, 785
AluI AGCT 9 cut(s) 7, 79, 143, 317, 373, 407, 626, 754, 785
Alw26I GTCTC 2 cut(s) 20, 534
Ama87I CYCGRG 2 cut(s) 55, 225
Aor13HI TCCGGA 1 cut(s) 401
ApeKI GCWGC 2 cut(s) 215, 317
AspS9I GGNCC 2 cut(s) 494, 710
AsuC2I CCSGG 1 cut(s) 498
AsuHPI GGTGA 1 cut(s) 558
AsuII TTCGAA 1 cut(s) 410
AsuNHI GCTAGC 2 cut(s) 230, 692
AvaI CYCGRG 2 cut(s) 55, 225
AvaII GGWCC 2 cut(s) 494, 710
BanI GGYRCC 1 cut(s) 778
BbvI GCAGC 2 cut(s) 202, 304
BccI CCATC 4 cut(s) 119, 474, 665, 714
BceAI ACGGC 2 cut(s) 345, 545
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 2 cut(s) 20, 534
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 3 cut(s) 15, 231, 693
BisI GCNGC 2 cut(s) 216, 318
BlsI GCNGC 2 cut(s) 217, 319
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 2 cut(s) 494, 710
BmeT110I CYCGRG 2 cut(s) 55, 225
BmgT120I GGNCC 2 cut(s) 494, 710
BmiI GGNNCC 4 cut(s) 496, 676, 712, 780
BmrFI CCNGG 1 cut(s) 498
BmtI GCTAGC 2 cut(s) 234, 696
BplI GAGNNNNNCTC 2 cut(s) 516, 548
BpmI CTGGAG 1 cut(s) 330
Bpu14I TTCGAA 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 1 cut(s) 498
BsaJI CCNNGG 2 cut(s) 442, 774
BsaWI WCCGGW 1 cut(s) 401
BsaXI ACNNNNNCTCC 2 cut(s) 729, 759
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 448, 720
Bse1I ACTGG 2 cut(s) 496, 678
BseAI TCCGGA 1 cut(s) 401
BseDI CCNNGG 2 cut(s) 442, 774
BseGI GGATG 2 cut(s) 485, 725
BseLI CCNNNNNNNGG 3 cut(s) 44, 448, 720
BseNI ACTGG 2 cut(s) 496, 678
BseRI GAGGAG 3 cut(s) 241, 327, 400
BseXI GCAGC 2 cut(s) 202, 304
BshNI GGYRCC 1 cut(s) 778
BsiHKCI CYCGRG 2 cut(s) 55, 225
BsiSI CCGG 2 cut(s) 402, 498
BslFI GGGAC 4 cut(s) 465, 480, 497, 696
BslI CCNNNNNNNGG 3 cut(s) 44, 448, 720
BsmAI GTCTC 2 cut(s) 20, 534
BsmFI GGGAC 4 cut(s) 465, 480, 497, 696
BsoBI CYCGRG 2 cut(s) 55, 225
Bsp119I TTCGAA 1 cut(s) 410
Bsp13I TCCGGA 1 cut(s) 401
Bsp143I GATC 2 cut(s) 100, 644
BspACI CCGC 2 cut(s) 594, 789
BspEI TCCGGA 1 cut(s) 401
BspLI GGNNCC 4 cut(s) 496, 676, 712, 780
BspOI GCTAGC 2 cut(s) 234, 696
BspT104I TTCGAA 1 cut(s) 410
BspT107I GGYRCC 1 cut(s) 778
BsrI ACTGG 2 cut(s) 496, 678
BssECI CCNNGG 2 cut(s) 442, 774
BssMI GATC 2 cut(s) 100, 644
Bst4CI ACNGT 2 cut(s) 443, 581
BstBI TTCGAA 1 cut(s) 410
BstC8I GCNNGC 4 cut(s) 162, 232, 375, 694
BstDSI CCRYGG 2 cut(s) 442, 774
BstF5I GGATG 2 cut(s) 485, 725
BstKTI GATC 2 cut(s) 103, 647
BstMAI GTCTC 2 cut(s) 20, 534
BstMBI GATC 2 cut(s) 100, 644
BstMWI GCNNNNNNNGC 2 cut(s) 76, 314
BstNSI RCATGY 3 cut(s) 154, 164, 430
BstSCI CCNGG 1 cut(s) 496
BstV1I GCAGC 2 cut(s) 202, 304
BtgI CCRYGG 2 cut(s) 442, 774
BtsCI GGATG 2 cut(s) 485, 725
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 2 cut(s) 67, 685
Cac8I GCNNGC 4 cut(s) 162, 232, 375, 694
Cfr13I GGNCC 2 cut(s) 494, 710
Csp6I GTAC 6 cut(s) 89, 111, 300, 465, 598, 700
CviAII CATG 8 cut(s) 34, 151, 161, 235, 368, 427, 656, 802
CviQI GTAC 6 cut(s) 89, 111, 300, 465, 598, 700
DpnI GATC 2 cut(s) 102, 646
DpnII GATC 2 cut(s) 100, 644
Eco47I GGWCC 2 cut(s) 494, 710
Eco88I CYCGRG 2 cut(s) 55, 225
FaeI CATG 8 cut(s) 37, 154, 164, 238, 371, 430, 659, 805
FaqI GGGAC 4 cut(s) 465, 480, 497, 696
FatI CATG 8 cut(s) 33, 150, 160, 234, 367, 426, 655, 801
FauI CCCGC 1 cut(s) 587
FauNDI CATATG 1 cut(s) 571
FblI GTMKAC 2 cut(s) 364, 513
Fnu4HI GCNGC 2 cut(s) 216, 318
FokI GGATG 2 cut(s) 492, 732
Fsp4HI GCNGC 2 cut(s) 216, 318
FspBI CTAG 3 cut(s) 15, 231, 693
GluI GCNGC 2 cut(s) 216, 318
GsuI CTGGAG 1 cut(s) 330
HapII CCGG 2 cut(s) 402, 498
Hin1II CATG 8 cut(s) 37, 154, 164, 238, 371, 430, 659, 805
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 2 cut(s) 5, 624
HinfI GANTC 2 cut(s) 221, 390
HpaII CCGG 2 cut(s) 402, 498
HphI GGTGA 1 cut(s) 558
Hpy166II GTNNAC 4 cut(s) 365, 514, 566, 700
Hpy188I TCNGA 2 cut(s) 135, 631
Hpy188III TCNNGA 4 cut(s) 25, 402, 713, 735
Hpy8I GTNNAC 4 cut(s) 365, 514, 566, 700
HpyAV CCTTC 2 cut(s) 515, 710
HpyCH4III ACNGT 2 cut(s) 443, 581
HpyCH4IV ACGT 1 cut(s) 705
HpyCH4V TGCA 1 cut(s) 164
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 314
HpySE526I ACGT 1 cut(s) 705
Hsp92II CATG 8 cut(s) 37, 154, 164, 238, 371, 430, 659, 805
Kpn2I TCCGGA 1 cut(s) 401
Kzo9I GATC 2 cut(s) 100, 644
LmnI GCTCC 4 cut(s) 314, 404, 674, 751
Lsp1109I GCAGC 2 cut(s) 202, 304
MaeI CTAG 3 cut(s) 15, 231, 693
MaeII ACGT 1 cut(s) 705
MalI GATC 2 cut(s) 102, 646
MboI GATC 2 cut(s) 100, 644
MboII GAAGA 5 cut(s) 75, 184, 277, 280, 632
MluCI AATT 2 cut(s) 329, 351
MnlI CCTC 7 cut(s) 103, 233, 262, 305, 378, 518, 799
MroI TCCGGA 1 cut(s) 401
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 594
MspI CCGG 2 cut(s) 402, 498
MspR9I CCNGG 1 cut(s) 498
MwoI GCNNNNNNNGC 2 cut(s) 76, 314
NciI CCSGG 1 cut(s) 498
NdeI CATATG 1 cut(s) 571
NdeII GATC 2 cut(s) 100, 644
NheI GCTAGC 2 cut(s) 230, 692
NlaIII CATG 8 cut(s) 37, 154, 164, 238, 371, 430, 659, 805
NlaIV GGNNCC 4 cut(s) 496, 676, 712, 780
NspI RCATGY 3 cut(s) 154, 164, 430
NspV TTCGAA 1 cut(s) 410
PaeI GCATGC 1 cut(s) 164
PaeR7I CTCGAG 1 cut(s) 55
PciI ACATGT 1 cut(s) 150
PcsI WCGNNNNNNNCGW 1 cut(s) 711
PfeI GAWTC 2 cut(s) 221, 390
PfoI TCCNGGA 1 cut(s) 496
PkrI GCNGC 2 cut(s) 217, 319
PscI ACATGT 1 cut(s) 150
PspN4I GGNNCC 4 cut(s) 496, 676, 712, 780
PspPI GGNCC 2 cut(s) 494, 710
PspXI VCTCGAGB 1 cut(s) 55
RsaI GTAC 6 cut(s) 90, 112, 301, 466, 599, 701
RsaNI GTAC 6 cut(s) 89, 111, 300, 465, 598, 700
RseI CAYNNNNRTG 1 cut(s) 38
SalI GTCGAC 1 cut(s) 512
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 2 cut(s) 216, 318
Sau3AI GATC 2 cut(s) 100, 644
Sau96I GGNCC 2 cut(s) 494, 710
ScrFI CCNGG 1 cut(s) 498
Sfr274I CTCGAG 1 cut(s) 55
SfuI TTCGAA 1 cut(s) 410
SinI GGWCC 2 cut(s) 494, 710
SlaI CTCGAG 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 55, 533
SmoI CTYRAG 2 cut(s) 55, 533
SpeI ACTAGT 1 cut(s) 14
SphI GCATGC 1 cut(s) 164
Sse9I AATT 2 cut(s) 329, 351
SsiI CCGC 2 cut(s) 594, 789
SspMI CTAG 3 cut(s) 15, 231, 693
StyD4I CCNGG 1 cut(s) 496
TaaI ACNGT 2 cut(s) 443, 581
TaiI ACGT 1 cut(s) 708
TaqI TCGA 4 cut(s) 24, 56, 410, 513
TasI AATT 2 cut(s) 329, 351
TfiI GAWTC 2 cut(s) 221, 390
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 2 cut(s) 67, 685
TseI GCWGC 2 cut(s) 215, 317
TspDTI ATGAA 2 cut(s) 17, 426
TspGWI ACGGA 1 cut(s) 656
TspRI CASTG 2 cut(s) 67, 685
VpaK11BI GGWCC 2 cut(s) 494, 710
XceI RCATGY 3 cut(s) 154, 164, 430
XhoI CTCGAG 1 cut(s) 55
XmiI GTMKAC 2 cut(s) 364, 513
XspI CTAG 3 cut(s) 15, 231, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.