Rroxscaffold_6G00429850

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000613
Physical Location & Seq
Forward (+)
50052009 .. 50052945
937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00429850.1

Sequence Viewer

Length: 585 bp
ATGATCTATAATAACTTTGGTGTAGTCGTCATCAATGATTCAATGGGCTCACTTTTGTTTTCGTTTTCGTTTTCTTCTGGGCCCCAATCGACAGTTGAGTTTGTGATACATGCGTATAAAACCACGTCATATCTCGAGACCTACAATGCTTTGCAAGTCTACCCCAAATTTTATAACCTTATGCCTAGAGGTGCAGATGACGTTCATACCGGAACATTTTTCGTGCTTTACCCTACGAATTCCTATTCCAAACTTGTGTGGGAACTGGGAAGGCCGACGGGAGATATAGACTTACAGCAACACCTAGAAGCAAGCCATTTTGTAGCAAAGAAGTACGATTTCTACTTTCACCGTTTCGTGTTCAACCAAGTAAACAAGGCTATACAAGCATACAATACTATCACTACCATAGCTCCTCCCCTACGTCATAACGCTATAGAAGATTATTTCCAAGGCAATTTTAAACGTTCTGGCAGCCAAAAGAAGCCCGTTTATATTGGATGGAGCTCAGAAGACTTGTTGAAACGAATCTGTAACATGAAGGATGCTATTAATTCATGGTATTGCAAGTATTTGACCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

22.51

Weight (kDa)

8.72

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
AccI GTMKAC 1 cut(s) 159
AclI AACGTT 1 cut(s) 466
AcsI RAATTY 2 cut(s) 167, 238
AfaI GTAC 1 cut(s) 335
AgsI TTSAA 3 cut(s) 42, 364, 523
AjiI CACGTC 1 cut(s) 126
AluBI AGCT 2 cut(s) 413, 507
AluI AGCT 2 cut(s) 413, 507
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 2 cut(s) 80, 272
ApaI GGGCCC 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 474
ApoI RAATTY 2 cut(s) 167, 238
AseI ATTAAT 1 cut(s) 552
AspS9I GGNCC 2 cut(s) 80, 81
AsuHPI GGTGA 1 cut(s) 341
AvaI CYCGRG 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 84
BaeI ACNNNNGTAYC 2 cut(s) 98, 131
BanII GRGCYC 3 cut(s) 50, 84, 509
BarI GAAGNNNNNNTAC 1 cut(s) 563
BbsI GAAGAC 1 cut(s) 519
Bbv12I GWGCWC 1 cut(s) 509
BbvI GCAGC 1 cut(s) 486
BccI CCATC 1 cut(s) 495
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 186, 305
BfmI CTRYAG 1 cut(s) 435
BisI GCNGC 1 cut(s) 475
BlsI GCNGC 1 cut(s) 476
BmeT110I CYCGRG 1 cut(s) 134
BmgBI CACGTC 1 cut(s) 126
BmgT120I GGNCC 2 cut(s) 80, 81
BmiI GGNNCC 2 cut(s) 82, 83
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 535
BmuI ACTGGG 1 cut(s) 275
BpiI GAAGAC 1 cut(s) 519
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 209
BsaXI ACNNNNNCTCC 2 cut(s) 397, 427
Bse1I ACTGG 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 2 cut(s) 506, 550
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 1 cut(s) 270
BseRI GAGGAG 1 cut(s) 405
BseSI GKGCMC 1 cut(s) 84
BseXI GCAGC 1 cut(s) 486
BsgI GTGCAG 1 cut(s) 213
BshFI GGCC 2 cut(s) 82, 274
BsiHKAI GWGCWC 1 cut(s) 509
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 1 cut(s) 210
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 2 cut(s) 82, 274
Bso31I GGTCTC 1 cut(s) 131
BsoBI CYCGRG 1 cut(s) 134
Bsp120I GGGCCC 1 cut(s) 80
Bsp1286I GDGCHC 3 cut(s) 50, 84, 509
Bsp143I GATC 1 cut(s) 3
BspANI GGCC 2 cut(s) 82, 274
BspCNI CTCAG 1 cut(s) 521
BspLI GGNNCC 2 cut(s) 82, 83
BspTNI GGTCTC 1 cut(s) 131
BsrI ACTGG 1 cut(s) 270
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 1 cut(s) 3
BssT1I CCWWGG 1 cut(s) 451
Bst4CI ACNGT 2 cut(s) 94, 353
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 1 cut(s) 508
BstF5I GGATG 2 cut(s) 506, 550
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNSI RCATGY 1 cut(s) 113
BstSFI CTRYAG 1 cut(s) 435
BstSLI GKGCMC 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 486
BstV2I GAAGAC 1 cut(s) 519
BsuRI GGCC 2 cut(s) 82, 274
BtrI CACGTC 1 cut(s) 126
BtsCI GGATG 2 cut(s) 506, 550
Cac8I GCNNGC 1 cut(s) 313
Cfr13I GGNCC 2 cut(s) 80, 81
Csp6I GTAC 1 cut(s) 334
CviAII CATG 3 cut(s) 110, 538, 558
CviJI RGCY 9 cut(s) 48, 82, 274, 315, 380, 413, 477, 487, 507
CviKI_1 RGCY 9 cut(s) 48, 82, 274, 315, 380, 413, 477, 487, 507
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 1 cut(s) 508
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
DraI TTTAAA 1 cut(s) 463
Ecl136II GAGCTC 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 3 cut(s) 50, 84, 509
Eco31I GGTCTC 1 cut(s) 131
Eco53kI GAGCTC 1 cut(s) 507
Eco88I CYCGRG 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 507
EcoO109I RGGNCCY 1 cut(s) 81
EcoRI GAATTC 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 3 cut(s) 50, 84, 509
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 3 cut(s) 113, 541, 561
FatI CATG 3 cut(s) 109, 537, 557
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 475
FokI GGATG 2 cut(s) 513, 557
FriOI GRGCYC 3 cut(s) 50, 84, 509
Fsp4HI GCNGC 1 cut(s) 475
FspBI CTAG 2 cut(s) 186, 305
GluI GCNGC 1 cut(s) 475
HaeIII GGCC 2 cut(s) 82, 274
HapII CCGG 1 cut(s) 210
Hin1II CATG 3 cut(s) 113, 541, 561
HinfI GANTC 2 cut(s) 38, 528
HpaII CCGG 1 cut(s) 210
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 2 cut(s) 160, 373
Hpy188I TCNGA 1 cut(s) 511
Hpy188III TCNNGA 2 cut(s) 134, 136
Hpy8I GTNNAC 2 cut(s) 160, 373
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 2 cut(s) 264, 535
HpyCH4III ACNGT 2 cut(s) 94, 353
HpyCH4IV ACGT 4 cut(s) 125, 201, 424, 466
HpyCH4V TGCA 3 cut(s) 154, 194, 567
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 1 cut(s) 508
HpySE526I ACGT 4 cut(s) 125, 201, 424, 466
Hsp92II CATG 3 cut(s) 113, 541, 561
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 2 cut(s) 418, 504
LpnPI CCDG 4 cut(s) 63, 223, 251, 456
Lsp1109I GCAGC 1 cut(s) 486
LweI GCATC 1 cut(s) 535
MaeI CTAG 2 cut(s) 186, 305
MaeII ACGT 4 cut(s) 125, 201, 424, 466
MaeIII GTNAC 1 cut(s) 533
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 3 cut(s) 66, 452, 524
MhlI GDGCHC 3 cut(s) 50, 84, 509
MluCI AATT 4 cut(s) 167, 238, 457, 553
MnlI CCTC 2 cut(s) 182, 426
MseI TTAA 2 cut(s) 462, 552
MspI CCGG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 386
NdeII GATC 1 cut(s) 3
NlaIII CATG 3 cut(s) 113, 541, 561
NlaIV GGNNCC 2 cut(s) 82, 83
NspI RCATGY 1 cut(s) 113
PaeR7I CTCGAG 1 cut(s) 134
PfeI GAWTC 2 cut(s) 38, 528
PkrI GCNGC 1 cut(s) 476
PshBI ATTAAT 1 cut(s) 552
PsiI TTATAA 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 509
Psp1406I AACGTT 1 cut(s) 466
PspN4I GGNNCC 2 cut(s) 82, 83
PspOMI GGGCCC 1 cut(s) 80
PspPI GGNCC 2 cut(s) 80, 81
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SacI GAGCTC 1 cut(s) 509
SaqAI TTAA 2 cut(s) 462, 552
SatI GCNGC 1 cut(s) 475
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 2 cut(s) 80, 81
SduI GDGCHC 3 cut(s) 50, 84, 509
SfaNI GCATC 1 cut(s) 535
SfcI CTRYAG 1 cut(s) 435
Sfr274I CTCGAG 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 4 cut(s) 167, 238, 457, 553
SspMI CTAG 2 cut(s) 186, 305
SstI GAGCTC 1 cut(s) 509
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 2 cut(s) 94, 353
TaiI ACGT 4 cut(s) 128, 204, 427, 469
TaqI TCGA 2 cut(s) 89, 135
TasI AATT 4 cut(s) 167, 238, 457, 553
TfiI GAWTC 2 cut(s) 38, 528
Tru1I TTAA 2 cut(s) 462, 552
Tru9I TTAA 2 cut(s) 462, 552
TseI GCWGC 1 cut(s) 474
TspDTI ATGAA 3 cut(s) 194, 546, 554
VspI ATTAAT 1 cut(s) 552
XapI RAATTY 2 cut(s) 167, 238
XceI RCATGY 1 cut(s) 113
XhoI CTCGAG 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 2 cut(s) 186, 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.