Rmu_sc0000131.1_g000009

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000131.1
OG0000613
Physical Location & Seq
Forward (+)
28243 .. 30692
2450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000131.1_g000009.1.cds

Sequence Viewer

Length: 897 bp
atgaagttcgacttcagcagtcttcaaaccactgcgccgttgctcggcgacagcagcggtgggttttcagctgcgccgtcgttcgaggtccctgttactgatgactttgatgggtttcagaaagaggctatccagatggtgaagccggccaaggggactacaacccttgcatttattttcaaggatggtgttatggtggcggctgattctcgagctagcatgggaggatatatatcgtctcagtctgtgaagaagattatcgaaatcaatccgtacatgcttggaactatggctggtggagctgctgactgtcagttttggcataggaatttggggattaagtgcagacttcacgaattggcaaacaagcgcagaatttcagttacaggtgcatccaagctcttggccaacattctgtactcttaccgtggaatgggtctgtctgttgggaccatgatagctgggtgggatgaaacgggtcctggactttattatgtggacagtgagggaggaaggttgaaagggatgcggttttcagttggatctggttcaccatatgcttatggtgtcctggataatggtgccaaagggttaactctttcttttcctagtgttgtagagttatttgtgtacatgatatgtctacttaccgtggttgcatatgccaggtaccgctatgatatgtcagttgaagaagctgctgagttagctaggagagccatatatcatgcaacattccgtgatggagctagtggtggagttgccagtgtttaccatgtcggtccaaatgggtggaaaaagctatctggagatgatgtcggggaacttcactacacatactatcctgtaaccccggagaccgtggaacaagaaatggtggaggtaggtgcagcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

298

Amino Acids

32.13

Weight (kDa)

5.98

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 669
AccB1I GGYRCC 2 cut(s) 581, 669
AccI GTMKAC 1 cut(s) 643
AciI CCGC 4 cut(s) 57, 200, 529, 673
AclWI GGATC 1 cut(s) 550
AcoI YGGCCR 2 cut(s) 147, 405
AcsI RAATTY 2 cut(s) 328, 375
AfaI GTAC 4 cut(s) 275, 419, 632, 671
AfiI CCNNNNNNNGG 3 cut(s) 44, 152, 433
AgsI TTSAA 4 cut(s) 26, 181, 520, 692
AjnI CCWGG 3 cut(s) 481, 570, 665
AluBI AGCT 9 cut(s) 71, 215, 302, 400, 461, 698, 710, 749, 802
AluI AGCT 9 cut(s) 71, 215, 302, 400, 461, 698, 710, 749, 802
Alw26I GTCTC 2 cut(s) 243, 851
AlwI GGATC 1 cut(s) 550
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 2 cut(s) 147, 405
ApeKI GCWGC 5 cut(s) 54, 71, 302, 698, 890
ApoI RAATTY 2 cut(s) 328, 375
Asp718I GGTACC 1 cut(s) 669
AspLEI GCGC 3 cut(s) 37, 76, 372
AspS9I GGNCC 4 cut(s) 88, 450, 479, 782
AsuC2I CCSGG 1 cut(s) 854
AsuHPI GGTGA 2 cut(s) 151, 543
AsuNHI GCTAGC 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 210
AvaII GGWCC 4 cut(s) 88, 450, 479, 782
BalI TGGCCA 1 cut(s) 407
BanI GGYRCC 2 cut(s) 581, 669
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 4 cut(s) 58, 66, 289, 685
BccI CCATC 4 cut(s) 104, 130, 179, 737
BceAI ACGGC 2 cut(s) 22, 61
BciT130I CCWGG 3 cut(s) 483, 572, 667
BcnI CCSGG 1 cut(s) 854
BcoDI GTCTC 2 cut(s) 243, 851
BfaI CTAG 4 cut(s) 216, 609, 711, 750
BisI GCNGC 6 cut(s) 55, 72, 201, 303, 699, 891
BlsI GCNGC 6 cut(s) 56, 73, 202, 304, 700, 892
Bme1390I CCNGG 4 cut(s) 483, 572, 667, 854
Bme18I GGWCC 4 cut(s) 88, 450, 479, 782
BmeT110I CYCGRG 1 cut(s) 210
BmgT120I GGNCC 4 cut(s) 88, 450, 479, 782
BmiI GGNNCC 5 cut(s) 90, 451, 480, 583, 671
BmrFI CCNGG 4 cut(s) 483, 572, 667, 854
BmsI GCATC 2 cut(s) 401, 516
BmtI GCTAGC 1 cut(s) 219
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 828
BpuMI CCSGG 1 cut(s) 854
BsaBI GATNNNNATC 1 cut(s) 232
BsaI GGTCTC 1 cut(s) 851
BsaJI CCNNGG 5 cut(s) 150, 427, 651, 852, 861
BsaXI ACNNNNNCTCC 2 cut(s) 801, 831
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 152, 433
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 765
Bse8I GATNNNNATC 1 cut(s) 232
BseBI CCWGG 3 cut(s) 483, 572, 667
BseDI CCNNGG 5 cut(s) 150, 427, 651, 852, 861
BseGI GGATG 4 cut(s) 190, 392, 475, 531
BseJI GATNNNNATC 1 cut(s) 232
BseLI CCNNNNNNNGG 3 cut(s) 44, 152, 433
BseMII CTCAG 2 cut(s) 254, 693
BseNI ACTGG 1 cut(s) 765
BseXI GCAGC 4 cut(s) 58, 66, 289, 685
BseYI CCCAGC 1 cut(s) 461
BsgI GTGCAG 1 cut(s) 364
BshFI GGCC 2 cut(s) 149, 407
BshNI GGYRCC 2 cut(s) 581, 669
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 2 cut(s) 146, 854
BslFI GGGAC 3 cut(s) 74, 169, 463
BslI CCNNNNNNNGG 3 cut(s) 44, 152, 433
BsmAI GTCTC 2 cut(s) 243, 851
BsmBI CGTCTC 1 cut(s) 243
BsmFI GGGAC 3 cut(s) 74, 169, 463
BsnI GGCC 2 cut(s) 149, 407
Bso31I GGTCTC 1 cut(s) 851
BsoBI CYCGRG 1 cut(s) 210
Bsp1407I TGTACA 1 cut(s) 630
Bsp143I GATC 1 cut(s) 542
BspACI CCGC 4 cut(s) 57, 200, 529, 673
BspANI GGCC 2 cut(s) 149, 407
BspCNI CTCAG 2 cut(s) 253, 694
BspLI GGNNCC 5 cut(s) 90, 451, 480, 583, 671
BspOI GCTAGC 1 cut(s) 219
BspPI GGATC 1 cut(s) 550
BspT107I GGYRCC 2 cut(s) 581, 669
BspTNI GGTCTC 1 cut(s) 851
BsrFI RCCGGY 1 cut(s) 145
BsrGI TGTACA 1 cut(s) 630
BsrI ACTGG 1 cut(s) 765
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 5 cut(s) 150, 427, 651, 852, 861
BssMI GATC 1 cut(s) 542
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 3 cut(s) 483, 572, 667
Bst4CI ACNGT 5 cut(s) 311, 428, 503, 652, 862
BstAUI TGTACA 1 cut(s) 630
BstC8I GCNNGC 2 cut(s) 147, 217
BstDEI CTNAG 2 cut(s) 240, 702
BstDSI CCRYGG 3 cut(s) 427, 651, 861
BstF5I GGATG 4 cut(s) 190, 392, 475, 531
BstHHI GCGC 3 cut(s) 37, 76, 372
BstKTI GATC 1 cut(s) 545
BstMAI GTCTC 2 cut(s) 243, 851
BstMBI GATC 1 cut(s) 542
BstMWI GCNNNNNNNGC 4 cut(s) 54, 299, 707, 716
BstNI CCWGG 3 cut(s) 483, 572, 667
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 4 cut(s) 481, 570, 665, 852
BstV1I GCAGC 4 cut(s) 58, 66, 289, 685
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 542
BstXI CCANNNNNNTGG 2 cut(s) 403, 792
BstYI RGATCY 1 cut(s) 542
BsuRI GGCC 2 cut(s) 149, 407
BtgI CCRYGG 3 cut(s) 427, 651, 861
BtsCI GGATG 4 cut(s) 190, 392, 475, 531
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 3 cut(s) 30, 508, 772
Cac8I GCNNGC 2 cut(s) 147, 217
CfoI GCGC 3 cut(s) 37, 76, 372
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 4 cut(s) 88, 450, 479, 782
Csp6I GTAC 4 cut(s) 274, 418, 631, 670
CviAII CATG 6 cut(s) 220, 277, 454, 634, 728, 776
CviQI GTAC 4 cut(s) 274, 418, 631, 670
DdeI CTNAG 2 cut(s) 240, 702
DpnI GATC 1 cut(s) 544
DpnII GATC 1 cut(s) 542
EaeI YGGCCR 2 cut(s) 147, 405
Eco130I CCWWGG 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 851
Eco47I GGWCC 4 cut(s) 88, 450, 479, 782
Eco88I CYCGRG 1 cut(s) 210
EcoO109I RGGNCCY 2 cut(s) 88, 479
EcoRII CCWGG 3 cut(s) 481, 570, 665
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 243
FaeI CATG 6 cut(s) 223, 280, 457, 637, 731, 779
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 3 cut(s) 74, 169, 463
FatI CATG 6 cut(s) 219, 276, 453, 633, 727, 775
FauNDI CATATG 2 cut(s) 556, 661
FblI GTMKAC 1 cut(s) 643
Fnu4HI GCNGC 6 cut(s) 55, 72, 201, 303, 699, 891
FokI GGATG 4 cut(s) 197, 379, 482, 538
Fsp4HI GCNGC 6 cut(s) 55, 72, 201, 303, 699, 891
FspBI CTAG 4 cut(s) 216, 609, 711, 750
GlaI GCGC 3 cut(s) 36, 75, 371
GluI GCNGC 6 cut(s) 55, 72, 201, 303, 699, 891
GsaI CCCAGC 1 cut(s) 465
GsuI CTGGAG 1 cut(s) 828
HaeIII GGCC 2 cut(s) 149, 407
HapII CCGG 2 cut(s) 146, 854
HhaI GCGC 3 cut(s) 37, 76, 372
Hin1II CATG 6 cut(s) 223, 280, 457, 637, 731, 779
Hin6I GCGC 3 cut(s) 35, 74, 370
HinP1I GCGC 3 cut(s) 35, 74, 370
HincII GTYRAC 1 cut(s) 594
HindII GTYRAC 1 cut(s) 594
HinfI GANTC 1 cut(s) 206
HpaI GTTAAC 1 cut(s) 594
HpaII CCGG 2 cut(s) 146, 854
HphI GGTGA 2 cut(s) 151, 543
Hpy166II GTNNAC 6 cut(s) 499, 551, 594, 631, 644, 772
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 4 cut(s) 133, 210, 353, 807
Hpy8I GTNNAC 6 cut(s) 499, 551, 594, 631, 644, 772
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 1 cut(s) 507
HpyCH4III ACNGT 5 cut(s) 311, 428, 503, 652, 862
HpyCH4V TGCA 6 cut(s) 170, 345, 392, 659, 731, 890
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 299, 707, 716
HpyF3I CTNAG 2 cut(s) 240, 702
Hsp92II CATG 6 cut(s) 223, 280, 457, 637, 731, 779
HspAI GCGC 3 cut(s) 35, 74, 370
KpnI GGTACC 1 cut(s) 673
KroI GCCGGC 1 cut(s) 145
KroNI GCCGGC 1 cut(s) 147
KspAI GTTAAC 1 cut(s) 594
Kzo9I GATC 1 cut(s) 542
LmnI GCTCC 2 cut(s) 299, 746
Lsp1109I GCAGC 4 cut(s) 58, 66, 289, 685
LweI GCATC 2 cut(s) 401, 516
MaeI CTAG 4 cut(s) 216, 609, 711, 750
MaeIII GTNAC 3 cut(s) 94, 382, 847
MalI GATC 1 cut(s) 544
MboI GATC 1 cut(s) 542
MboII GAAGA 4 cut(s) 14, 262, 265, 704
MflI RGATCY 1 cut(s) 542
MlsI TGGCCA 1 cut(s) 407
MluCI AATT 3 cut(s) 328, 356, 375
MluNI TGGCCA 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 6 cut(s) 79, 118, 218, 499, 503, 874
Mox20I TGGCCA 1 cut(s) 407
MroNI GCCGGC 1 cut(s) 145
MscI TGGCCA 1 cut(s) 407
MseI TTAA 2 cut(s) 339, 593
Msp20I TGGCCA 1 cut(s) 407
MspA1I CMGCKG 2 cut(s) 57, 71
MspI CCGG 2 cut(s) 146, 854
MspR9I CCNGG 4 cut(s) 483, 572, 667, 854
MvaI CCWGG 3 cut(s) 483, 572, 667
MwoI GCNNNNNNNGC 4 cut(s) 54, 299, 707, 716
NaeI GCCGGC 1 cut(s) 147
NciI CCSGG 1 cut(s) 854
NdeI CATATG 2 cut(s) 556, 661
NdeII GATC 1 cut(s) 542
NgoMIV GCCGGC 1 cut(s) 145
NheI GCTAGC 1 cut(s) 215
NlaIII CATG 6 cut(s) 223, 280, 457, 637, 731, 779
NlaIV GGNNCC 5 cut(s) 90, 451, 480, 583, 671
NmeAIII GCCGAG 1 cut(s) 24
NspI RCATGY 1 cut(s) 280
PaeR7I CTCGAG 1 cut(s) 210
PdiI GCCGGC 1 cut(s) 147
PfeI GAWTC 1 cut(s) 206
PfoI TCCNGGA 2 cut(s) 481, 570
PkrI GCNGC 6 cut(s) 56, 73, 202, 304, 700, 892
PpuMI RGGWCCY 2 cut(s) 88, 479
Psp5II RGGWCCY 2 cut(s) 88, 479
Psp6I CCWGG 3 cut(s) 481, 570, 665
PspFI CCCAGC 1 cut(s) 461
PspGI CCWGG 3 cut(s) 481, 570, 665
PspN4I GGNNCC 5 cut(s) 90, 451, 480, 583, 671
PspPI GGNCC 4 cut(s) 88, 450, 479, 782
PspPPI RGGWCCY 2 cut(s) 88, 479
PsuI RGATCY 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 4 cut(s) 275, 419, 632, 671
RsaNI GTAC 4 cut(s) 274, 418, 631, 670
SaqAI TTAA 2 cut(s) 339, 593
SatI GCNGC 6 cut(s) 55, 72, 201, 303, 699, 891
Sau3AI GATC 1 cut(s) 542
Sau96I GGNCC 4 cut(s) 88, 450, 479, 782
ScrFI CCNGG 4 cut(s) 483, 572, 667, 854
SfaNI GCATC 2 cut(s) 401, 516
Sfr274I CTCGAG 1 cut(s) 210
SinI GGWCC 4 cut(s) 88, 450, 479, 782
SlaI CTCGAG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
Sse9I AATT 3 cut(s) 328, 356, 375
SsiI CCGC 4 cut(s) 57, 200, 529, 673
SspMI CTAG 4 cut(s) 216, 609, 711, 750
StyD4I CCNGG 4 cut(s) 481, 570, 665, 852
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 5 cut(s) 311, 428, 503, 652, 862
TaqI TCGA 4 cut(s) 9, 84, 211, 261
TaqII GACCGA 1 cut(s) 770
TasI AATT 3 cut(s) 328, 356, 375
TatI WGTACW 2 cut(s) 417, 630
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 2 cut(s) 339, 593
Tru9I TTAA 2 cut(s) 339, 593
TscAI CASTG 3 cut(s) 37, 508, 772
TseI GCWGC 5 cut(s) 54, 71, 302, 698, 890
TspDTI ATGAA 2 cut(s) 17, 486
TspGWI ACGGA 2 cut(s) 261, 728
TspRI CASTG 3 cut(s) 37, 508, 772
VpaK11BI GGWCC 4 cut(s) 88, 450, 479, 782
XapI RAATTY 2 cut(s) 328, 375
XceI RCATGY 1 cut(s) 280
XhoI CTCGAG 1 cut(s) 210
XmiI GTMKAC 1 cut(s) 643
XspI CTAG 4 cut(s) 216, 609, 711, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.