The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 104 |
| AccII | CGCG | 1 cut(s) | 96 |
| AciI | CCGC | 2 cut(s) | 435, 535 |
| AclWI | GGATC | 2 cut(s) | 415, 428 |
| AcsI | RAATTY | 4 cut(s) | 86, 472, 506, 704 |
| AfaI | GTAC | 1 cut(s) | 614 |
| AfeI | AGCGCT | 1 cut(s) | 150 |
| AgsI | TTSAA | 3 cut(s) | 188, 386, 554 |
| AluBI | AGCT | 4 cut(s) | 50, 325, 350, 687 |
| AluI | AGCT | 4 cut(s) | 50, 325, 350, 687 |
| Alw21I | GWGCWC | 1 cut(s) | 689 |
| AlwI | GGATC | 2 cut(s) | 415, 428 |
| Aor51HI | AGCGCT | 1 cut(s) | 150 |
| ApeKI | GCWGC | 2 cut(s) | 221, 322 |
| ApoI | RAATTY | 4 cut(s) | 86, 472, 506, 704 |
| AseI | ATTAAT | 1 cut(s) | 303 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 624 |
| AspLEI | GCGC | 1 cut(s) | 151 |
| AspS9I | GGNCC | 1 cut(s) | 80 |
| AsuC2I | CCSGG | 1 cut(s) | 418 |
| AsuHPI | GGTGA | 1 cut(s) | 527 |
| AsuII | TTCGAA | 1 cut(s) | 598 |
| AvaII | GGWCC | 1 cut(s) | 80 |
| BamHI | GGATCC | 1 cut(s) | 420 |
| BanII | GRGCYC | 1 cut(s) | 689 |
| Bbv12I | GWGCWC | 1 cut(s) | 689 |
| BbvI | GCAGC | 2 cut(s) | 208, 334 |
| BccI | CCATC | 4 cut(s) | 57, 309, 495, 733 |
| BcnI | CCSGG | 1 cut(s) | 418 |
| BfaI | CTAG | 2 cut(s) | 152, 617 |
| BfoI | RGCGCY | 1 cut(s) | 152 |
| BisI | GCNGC | 2 cut(s) | 222, 323 |
| BlsI | GCNGC | 2 cut(s) | 223, 324 |
| Bme1390I | CCNGG | 1 cut(s) | 418 |
| Bme18I | GGWCC | 1 cut(s) | 80 |
| BmgT120I | GGNCC | 1 cut(s) | 80 |
| BmiI | GGNNCC | 2 cut(s) | 81, 422 |
| BmrFI | CCNGG | 1 cut(s) | 418 |
| BmsI | GCATC | 4 cut(s) | 58, 208, 403, 491 |
| Bpu14I | TTCGAA | 1 cut(s) | 598 |
| BpuEI | CTTGAG | 1 cut(s) | 673 |
| BpuMI | CCSGG | 1 cut(s) | 418 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 571, 601 |
| BseGI | GGATG | 6 cut(s) | 31, 68, 73, 320, 418, 482 |
| BseRI | GAGGAG | 1 cut(s) | 579 |
| BseXI | GCAGC | 2 cut(s) | 208, 334 |
| Bsh1236I | CGCG | 1 cut(s) | 96 |
| BsiHKAI | GWGCWC | 1 cut(s) | 689 |
| BsiSI | CCGG | 1 cut(s) | 417 |
| BsmI | GAATGC | 1 cut(s) | 343 |
| Bsp119I | TTCGAA | 1 cut(s) | 598 |
| Bsp1286I | GDGCHC | 1 cut(s) | 689 |
| Bsp143I | GATC | 2 cut(s) | 420, 631 |
| BspACI | CCGC | 2 cut(s) | 435, 535 |
| BspFNI | CGCG | 1 cut(s) | 96 |
| BspHI | TCATGA | 1 cut(s) | 333 |
| BspLI | GGNNCC | 2 cut(s) | 81, 422 |
| BspPI | GGATC | 2 cut(s) | 415, 428 |
| BspQI | GCTCTTC | 1 cut(s) | 180 |
| BspT104I | TTCGAA | 1 cut(s) | 598 |
| BssMI | GATC | 2 cut(s) | 420, 631 |
| Bst4CI | ACNGT | 3 cut(s) | 377, 624, 670 |
| Bst6I | CTCTTC | 1 cut(s) | 180 |
| BstBI | TTCGAA | 1 cut(s) | 598 |
| BstF5I | GGATG | 6 cut(s) | 31, 68, 73, 320, 418, 482 |
| BstFNI | CGCG | 1 cut(s) | 96 |
| BstH2I | RGCGCY | 1 cut(s) | 152 |
| BstHHI | GCGC | 1 cut(s) | 151 |
| BstKTI | GATC | 2 cut(s) | 423, 634 |
| BstMBI | GATC | 2 cut(s) | 420, 631 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 148, 230, 347, 569 |
| BstNSI | RCATGY | 1 cut(s) | 470 |
| BstSCI | CCNGG | 1 cut(s) | 416 |
| BstUI | CGCG | 1 cut(s) | 96 |
| BstV1I | GCAGC | 2 cut(s) | 208, 334 |
| BstX2I | RGATCY | 1 cut(s) | 420 |
| BstYI | RGATCY | 1 cut(s) | 420 |
| BtgZI | GCGATG | 1 cut(s) | 77 |
| BtsCI | GGATG | 6 cut(s) | 31, 68, 73, 320, 418, 482 |
| BtsIMutI | CAGTG | 1 cut(s) | 210 |
| CciI | TCATGA | 1 cut(s) | 333 |
| CfoI | GCGC | 1 cut(s) | 151 |
| Cfr13I | GGNCC | 1 cut(s) | 80 |
| Csp6I | GTAC | 1 cut(s) | 613 |
| CviAII | CATG | 4 cut(s) | 170, 334, 467, 593 |
| CviJI | RGCY | 6 cut(s) | 50, 325, 350, 463, 497, 687 |
| CviKI_1 | RGCY | 6 cut(s) | 50, 325, 350, 463, 497, 687 |
| CviQI | GTAC | 1 cut(s) | 613 |
| DpnI | GATC | 2 cut(s) | 422, 633 |
| DpnII | GATC | 2 cut(s) | 420, 631 |
| DraI | TTTAAA | 1 cut(s) | 238 |
| Eam1104I | CTCTTC | 1 cut(s) | 180 |
| EarI | CTCTTC | 1 cut(s) | 180 |
| Ecl136II | GAGCTC | 1 cut(s) | 687 |
| Eco24I | GRGCYC | 1 cut(s) | 689 |
| Eco47I | GGWCC | 1 cut(s) | 80 |
| Eco47III | AGCGCT | 1 cut(s) | 150 |
| Eco53kI | GAGCTC | 1 cut(s) | 687 |
| EcoICRI | GAGCTC | 1 cut(s) | 687 |
| EcoO109I | RGGNCCY | 1 cut(s) | 80 |
| EcoT38I | GRGCYC | 1 cut(s) | 689 |
| FaeI | CATG | 4 cut(s) | 173, 337, 470, 596 |
| FatI | CATG | 4 cut(s) | 169, 333, 466, 592 |
| FauNDI | CATATG | 1 cut(s) | 574 |
| Fnu4HI | GCNGC | 2 cut(s) | 222, 323 |
| FokI | GGATG | 6 cut(s) | 38, 75, 80, 327, 425, 469 |
| FriOI | GRGCYC | 1 cut(s) | 689 |
| Fsp4HI | GCNGC | 2 cut(s) | 222, 323 |
| FspBI | CTAG | 2 cut(s) | 152, 617 |
| GlaI | GCGC | 1 cut(s) | 150 |
| GluI | GCNGC | 2 cut(s) | 222, 323 |
| HaeII | RGCGCY | 1 cut(s) | 152 |
| HapII | CCGG | 1 cut(s) | 417 |
| HhaI | GCGC | 1 cut(s) | 151 |
| Hin1II | CATG | 4 cut(s) | 173, 337, 470, 596 |
| Hin6I | GCGC | 1 cut(s) | 149 |
| HinP1I | GCGC | 1 cut(s) | 149 |
| HindIII | AAGCTT | 1 cut(s) | 348 |
| HinfI | GANTC | 2 cut(s) | 308, 655 |
| HpaII | CCGG | 1 cut(s) | 417 |
| HphI | GGTGA | 1 cut(s) | 527 |
| Hpy166II | GTNNAC | 1 cut(s) | 210 |
| Hpy188I | TCNGA | 1 cut(s) | 15 |
| Hpy188III | TCNNGA | 5 cut(s) | 23, 83, 312, 334, 629 |
| Hpy8I | GTNNAC | 1 cut(s) | 210 |
| HpyAV | CCTTC | 1 cut(s) | 380 |
| HpyCH4III | ACNGT | 3 cut(s) | 377, 624, 670 |
| HpyCH4V | TGCA | 2 cut(s) | 254, 470 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 148, 230, 347, 569 |
| Hsp92II | CATG | 4 cut(s) | 173, 337, 470, 596 |
| HspAI | GCGC | 1 cut(s) | 149 |
| Kzo9I | GATC | 2 cut(s) | 420, 631 |
| LguI | GCTCTTC | 1 cut(s) | 180 |
| LmnI | GCTCC | 3 cut(s) | 47, 330, 684 |
| LpnPI | CCDG | 8 cut(s) | 8, 96, 297, 342, 430, 473, 642, 686 |
| Lsp1109I | GCAGC | 2 cut(s) | 208, 334 |
| LweI | GCATC | 4 cut(s) | 58, 208, 403, 491 |
| MaeI | CTAG | 2 cut(s) | 152, 617 |
| MaeIII | GTNAC | 1 cut(s) | 664 |
| MalI | GATC | 2 cut(s) | 422, 633 |
| MboI | GATC | 2 cut(s) | 420, 631 |
| MboII | GAAGA | 2 cut(s) | 167, 566 |
| MflI | RGATCY | 1 cut(s) | 420 |
| MhlI | GDGCHC | 1 cut(s) | 689 |
| MluCI | AATT | 6 cut(s) | 73, 86, 472, 506, 679, 704 |
| MmeI | TCCRAC | 2 cut(s) | 40, 448 |
| MnlI | CCTC | 5 cut(s) | 125, 506, 509, 600, 619 |
| MroXI | GAANNNNTTC | 1 cut(s) | 624 |
| MseI | TTAA | 4 cut(s) | 237, 249, 303, 751 |
| MslI | CAYNNNNRTG | 2 cut(s) | 276, 303 |
| MspI | CCGG | 1 cut(s) | 417 |
| MspR9I | CCNGG | 1 cut(s) | 418 |
| Mva1269I | GAATGC | 1 cut(s) | 343 |
| MvnI | CGCG | 1 cut(s) | 96 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 148, 230, 347, 569 |
| NciI | CCSGG | 1 cut(s) | 418 |
| NdeI | CATATG | 1 cut(s) | 574 |
| NdeII | GATC | 2 cut(s) | 420, 631 |
| NlaIII | CATG | 4 cut(s) | 173, 337, 470, 596 |
| NlaIV | GGNNCC | 2 cut(s) | 81, 422 |
| NspI | RCATGY | 1 cut(s) | 470 |
| NspV | TTCGAA | 1 cut(s) | 598 |
| PagI | TCATGA | 1 cut(s) | 333 |
| PciSI | GCTCTTC | 1 cut(s) | 180 |
| PctI | GAATGC | 1 cut(s) | 343 |
| PdmI | GAANNNNTTC | 1 cut(s) | 624 |
| PfeI | GAWTC | 2 cut(s) | 308, 655 |
| PkrI | GCNGC | 2 cut(s) | 223, 324 |
| PpuMI | RGGWCCY | 1 cut(s) | 80 |
| PshBI | ATTAAT | 1 cut(s) | 303 |
| PsiI | TTATAA | 1 cut(s) | 104 |
| Psp124BI | GAGCTC | 1 cut(s) | 689 |
| Psp5II | RGGWCCY | 1 cut(s) | 80 |
| PspN4I | GGNNCC | 2 cut(s) | 81, 422 |
| PspPI | GGNCC | 1 cut(s) | 80 |
| PspPPI | RGGWCCY | 1 cut(s) | 80 |
| PsuI | RGATCY | 1 cut(s) | 420 |
| RsaI | GTAC | 1 cut(s) | 614 |
| RsaNI | GTAC | 1 cut(s) | 613 |
| RseI | CAYNNNNRTG | 2 cut(s) | 276, 303 |
| SacI | GAGCTC | 1 cut(s) | 689 |
| SapI | GCTCTTC | 1 cut(s) | 180 |
| SaqAI | TTAA | 4 cut(s) | 237, 249, 303, 751 |
| SatI | GCNGC | 2 cut(s) | 222, 323 |
| Sau3AI | GATC | 2 cut(s) | 420, 631 |
| Sau96I | GGNCC | 1 cut(s) | 80 |
| ScrFI | CCNGG | 1 cut(s) | 418 |
| SduI | GDGCHC | 1 cut(s) | 689 |
| SfaNI | GCATC | 4 cut(s) | 58, 208, 403, 491 |
| SfuI | TTCGAA | 1 cut(s) | 598 |
| SinI | GGWCC | 1 cut(s) | 80 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 276, 303 |
| SmlI | CTYRAG | 1 cut(s) | 688 |
| SmoI | CTYRAG | 1 cut(s) | 688 |
| Sse9I | AATT | 6 cut(s) | 73, 86, 472, 506, 679, 704 |
| SsiI | CCGC | 2 cut(s) | 435, 535 |
| SspMI | CTAG | 2 cut(s) | 152, 617 |
| SstI | GAGCTC | 1 cut(s) | 689 |
| StyD4I | CCNGG | 1 cut(s) | 416 |
| TaaI | ACNGT | 3 cut(s) | 377, 624, 670 |
| TaqI | TCGA | 3 cut(s) | 195, 546, 598 |
| TasI | AATT | 6 cut(s) | 73, 86, 472, 506, 679, 704 |
| TfiI | GAWTC | 2 cut(s) | 308, 655 |
| Tru1I | TTAA | 4 cut(s) | 237, 249, 303, 751 |
| Tru9I | TTAA | 4 cut(s) | 237, 249, 303, 751 |
| TscAI | CASTG | 1 cut(s) | 217 |
| TseI | GCWGC | 2 cut(s) | 221, 322 |
| TspDTI | ATGAA | 6 cut(s) | 80, 321, 350, 573, 591, 732 |
| TspGWI | ACGGA | 1 cut(s) | 403 |
| TspRI | CASTG | 1 cut(s) | 217 |
| VpaK11BI | GGWCC | 1 cut(s) | 80 |
| VspI | ATTAAT | 1 cut(s) | 303 |
| XapI | RAATTY | 4 cut(s) | 86, 472, 506, 704 |
| XceI | RCATGY | 1 cut(s) | 470 |
| XmnI | GAANNNNTTC | 1 cut(s) | 624 |
| XspI | CTAG | 2 cut(s) | 152, 617 |