pycom13g10110

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000613
Physical Location & Seq
Forward (+)
6771761 .. 6773976
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g10110.3

Sequence Viewer

Length: 810 bp
ATGAAGCTTGACACTACCGGTCTCGAAACACACGCGCCGTTGTGCCGCGAGAGCAGTGCGTTTCTCGACGGCGTTTCGGCTCCTCCGTCGTTCGAGGTTCCTACTACCAATGATTTTGATGGATTTCAGAAGGAAGCTATCCAGATGGTGAAGCCGGCCAAGGGGACAACCACGCTTGCGTTCATCTTCAAGGATGGCGTCATGGTGGCGGCCGATTCTCGGGCCAGCATGGGCGGCTATATCTCATCACAATCTGTGAAGAAGATTATTGAAATCAATCCTTACATGCTTGGTACAATGGCTGGAGGAGCTGCTGACTGCCAGTTTTGGCACAGAAATCTGGGGATCAAGTGTCGGCTGCATGAGTTGGCAAATAAGCGGAGGATTTCAGTTACTGGAGCATCAAAGCTGCTGGCAAACATTCTTTACTCTTATCGTGGTATGGGTCTCTCAGTTGGGACCATGATTGCAGGCTGGGACGAAACGGGTCCTGGACTTTACTATGTTGACAGTGAAGGGGGAAGATTGAAGGGGATGCGGTTTTCTGTTGGGTCTGGCTCTCCATATGCTTATGGTGTTCTGGATAATGGGTACCGATATGATATGTCTGTAGAAGAAGCTGCCGAGTTGGCTAGAAGAGCAATATATCATGCAACATTCCGCGATGGAGCCAGCGGCGGTGTTGCTAGCGTCTACCACGTGGGACCAAACGGATGGAAGAAGTTATCCGGAGACGATGTCGGGGAGCTTCACTACAGCTACTACCCTGCAGCGGCAGTCTCTGTTGAACAAGAAATGGTTGACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

270

Amino Acids

29.0

Weight (kDa)

6.08

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 807
Acc65I GGTACC 1 cut(s) 591
AccB1I GGYRCC 1 cut(s) 591
AccI GTMKAC 1 cut(s) 693
AccII CGCG 3 cut(s) 35, 48, 663
AccIII TCCGGA 1 cut(s) 728
AciI CCGC 9 cut(s) 46, 209, 234, 379, 538, 661, 675, 678, 773
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 2 cut(s) 156, 210
AcvI CACGTG 1 cut(s) 700
AcyI GRCGYC 2 cut(s) 198, 804
AfaI GTAC 2 cut(s) 295, 593
AfiI CCNNNNNNNGG 3 cut(s) 161, 219, 772
AgeI ACCGGT 1 cut(s) 17
AgsI TTSAA 4 cut(s) 190, 272, 529, 788
AjnI CCWGG 1 cut(s) 490
AluBI AGCT 7 cut(s) 7, 137, 311, 409, 620, 748, 759
AluI AGCT 7 cut(s) 7, 137, 311, 409, 620, 748, 759
Alw26I GTCTC 4 cut(s) 26, 452, 726, 784
AlwI GGATC 1 cut(s) 353
AlwNI CAGNNNCTG 2 cut(s) 395, 782
Ama87I CYCGRG 1 cut(s) 219
Aor13HI TCCGGA 1 cut(s) 728
AoxI GGCC 3 cut(s) 156, 210, 222
ApeKI GCWGC 5 cut(s) 311, 358, 409, 620, 770
AsiGI ACCGGT 1 cut(s) 17
Asp718I GGTACC 1 cut(s) 591
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 4 cut(s) 222, 459, 488, 704
AsuHPI GGTGA 1 cut(s) 160
AsuNHI GCTAGC 1 cut(s) 686
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 3 cut(s) 459, 488, 704
BanI GGYRCC 1 cut(s) 591
BbrPI CACGTG 1 cut(s) 700
BbvI GCAGC 5 cut(s) 298, 345, 396, 607, 782
BccI CCATC 5 cut(s) 113, 139, 188, 659, 708
BceAI ACGGC 2 cut(s) 22, 85
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 1 cut(s) 492
BcoDI GTCTC 4 cut(s) 26, 452, 726, 784
BfaI CTAG 2 cut(s) 633, 687
BfmI CTRYAG 3 cut(s) 609, 754, 768
BglI GCCNNNNNGGC 1 cut(s) 629
Bme1390I CCNGG 1 cut(s) 492
Bme18I GGWCC 3 cut(s) 459, 488, 704
BmeT110I CYCGRG 1 cut(s) 219
BmgT120I GGNCC 4 cut(s) 222, 459, 488, 704
BmiI GGNNCC 7 cut(s) 81, 99, 460, 489, 593, 670, 705
BmrFI CCNGG 1 cut(s) 492
BmsI GCATC 2 cut(s) 410, 525
BmtI GCTAGC 1 cut(s) 690
BpmI CTGGAG 2 cut(s) 324, 417
BsaAI YACGTR 1 cut(s) 700
BsaHI GRCGYC 2 cut(s) 198, 804
BsaI GGTCTC 2 cut(s) 26, 452
BsaJI CCNNGG 1 cut(s) 159
BsaWI WCCGGW 2 cut(s) 17, 728
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 219, 772
Bse118I RCCGGY 2 cut(s) 17, 154
Bse1I ACTGG 2 cut(s) 322, 400
BseAI TCCGGA 1 cut(s) 728
BseBI CCWGG 1 cut(s) 492
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 3 cut(s) 199, 540, 719
BseLI CCNNNNNNNGG 3 cut(s) 161, 219, 772
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 2 cut(s) 322, 400
BseRI GAGGAG 2 cut(s) 72, 321
BseX3I CGGCCG 1 cut(s) 210
BseXI GCAGC 5 cut(s) 298, 345, 396, 607, 782
BseYI CCCAGC 1 cut(s) 474
Bsh1236I CGCG 3 cut(s) 35, 48, 663
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 3 cut(s) 158, 212, 224
BshNI GGYRCC 1 cut(s) 591
BshTI ACCGGT 1 cut(s) 17
BsiEI CGRYCG 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 3 cut(s) 18, 155, 729
BslFI GGGAC 4 cut(s) 178, 472, 491, 717
BslI CCNNNNNNNGG 3 cut(s) 161, 219, 772
BsmAI GTCTC 4 cut(s) 26, 452, 726, 784
BsmBI CGTCTC 1 cut(s) 726
BsmFI GGGAC 4 cut(s) 178, 472, 491, 717
BsnI GGCC 3 cut(s) 158, 212, 224
Bso31I GGTCTC 2 cut(s) 26, 452
BsoBI CYCGRG 1 cut(s) 219
Bsp13I TCCGGA 1 cut(s) 728
Bsp143I GATC 1 cut(s) 345
BspACI CCGC 9 cut(s) 46, 209, 234, 379, 538, 661, 675, 678, 773
BspANI GGCC 3 cut(s) 158, 212, 224
BspCNI CTCAG 1 cut(s) 464
BspEI TCCGGA 1 cut(s) 728
BspFNI CGCG 3 cut(s) 35, 48, 663
BspLI GGNNCC 7 cut(s) 81, 99, 460, 489, 593, 670, 705
BspMAI CTGCAG 1 cut(s) 772
BspOI GCTAGC 1 cut(s) 690
BspPI GGATC 1 cut(s) 353
BspQI GCTCTTC 1 cut(s) 631
BspT107I GGYRCC 1 cut(s) 591
BspTNI GGTCTC 2 cut(s) 26, 452
BsrFI RCCGGY 2 cut(s) 17, 154
BsrI ACTGG 2 cut(s) 322, 400
BssAI RCCGGY 2 cut(s) 17, 154
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 1 cut(s) 345
BssNI GRCGYC 2 cut(s) 198, 804
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 1 cut(s) 512
Bst6I CTCTTC 1 cut(s) 631
BstACI GRCGYC 2 cut(s) 198, 804
BstBAI YACGTR 1 cut(s) 700
BstC8I GCNNGC 7 cut(s) 156, 177, 226, 414, 472, 673, 688
BstDEI CTNAG 1 cut(s) 451
BstF5I GGATG 3 cut(s) 199, 540, 719
BstFNI CGCG 3 cut(s) 35, 48, 663
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 1 cut(s) 348
BstMAI GTCTC 4 cut(s) 26, 452, 726, 784
BstMBI GATC 1 cut(s) 345
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 5 cut(s) 51, 234, 308, 629, 638
BstNI CCWGG 1 cut(s) 492
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 1 cut(s) 490
BstSFI CTRYAG 3 cut(s) 609, 754, 768
BstUI CGCG 3 cut(s) 35, 48, 663
BstV1I GCAGC 5 cut(s) 298, 345, 396, 607, 782
BstXI CCANNNNNNTGG 1 cut(s) 714
BstZI CGGCCG 1 cut(s) 210
BsuRI GGCC 3 cut(s) 158, 212, 224
BtgZI GCGATG 1 cut(s) 678
BtsCI GGATG 3 cut(s) 199, 540, 719
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 61, 517
Cac8I GCNNGC 7 cut(s) 156, 177, 226, 414, 472, 673, 688
CaiI CAGNNNCTG 2 cut(s) 395, 782
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 2 cut(s) 17, 154
Cfr13I GGNCC 4 cut(s) 222, 459, 488, 704
CseI GACGC 2 cut(s) 187, 679
Csp6I GTAC 2 cut(s) 294, 592
CspAI ACCGGT 1 cut(s) 17
CviAII CATG 6 cut(s) 202, 229, 286, 362, 463, 650
CviQI GTAC 2 cut(s) 294, 592
DdeI CTNAG 1 cut(s) 451
DpnI GATC 1 cut(s) 347
DpnII GATC 1 cut(s) 345
EaeI YGGCCR 2 cut(s) 156, 210
EagI CGGCCG 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 631
EarI CTCTTC 1 cut(s) 631
EclXI CGGCCG 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 159
Eco31I GGTCTC 2 cut(s) 26, 452
Eco47I GGWCC 3 cut(s) 459, 488, 704
Eco52I CGGCCG 1 cut(s) 210
Eco72I CACGTG 1 cut(s) 700
Eco88I CYCGRG 1 cut(s) 219
EcoO109I RGGNCCY 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 726
FaeI CATG 6 cut(s) 205, 232, 289, 365, 466, 653
FaqI GGGAC 4 cut(s) 178, 472, 491, 717
FatI CATG 6 cut(s) 201, 228, 285, 361, 462, 649
FauNDI CATATG 1 cut(s) 565
FblI GTMKAC 1 cut(s) 693
FokI GGATG 3 cut(s) 206, 547, 726
FspBI CTAG 2 cut(s) 633, 687
GlaI GCGC 1 cut(s) 36
GsaI CCCAGC 1 cut(s) 478
GsuI CTGGAG 2 cut(s) 324, 417
HaeIII GGCC 3 cut(s) 158, 212, 224
HapII CCGG 3 cut(s) 18, 155, 729
HgaI GACGC 2 cut(s) 187, 679
HhaI GCGC 1 cut(s) 37
Hin1I GRCGYC 2 cut(s) 198, 804
Hin1II CATG 6 cut(s) 205, 232, 289, 365, 466, 653
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 2 cut(s) 508, 802
HindII GTYRAC 2 cut(s) 508, 802
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 1 cut(s) 215
HpaII CCGG 3 cut(s) 18, 155, 729
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 3 cut(s) 508, 694, 802
Hpy188I TCNGA 2 cut(s) 129, 809
Hpy188III TCNNGA 5 cut(s) 23, 65, 142, 581, 729
Hpy8I GTNNAC 3 cut(s) 508, 694, 802
Hpy99I CGWCG 2 cut(s) 71, 91
HpyAV CCTTC 3 cut(s) 124, 509, 523
HpyCH4III ACNGT 1 cut(s) 512
HpyCH4IV ACGT 2 cut(s) 699, 804
HpyCH4V TGCA 4 cut(s) 361, 470, 653, 770
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 234, 308, 629, 638
HpyF3I CTNAG 1 cut(s) 451
HpySE526I ACGT 2 cut(s) 699, 804
Hsp92I GRCGYC 2 cut(s) 198, 804
Hsp92II CATG 6 cut(s) 205, 232, 289, 365, 466, 653
HspAI GCGC 1 cut(s) 35
Kpn2I TCCGGA 1 cut(s) 728
KpnI GGTACC 1 cut(s) 595
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Kzo9I GATC 1 cut(s) 345
LguI GCTCTTC 1 cut(s) 631
LmnI GCTCC 5 cut(s) 85, 308, 398, 668, 745
Lsp1109I GCAGC 5 cut(s) 298, 345, 396, 607, 782
LweI GCATC 2 cut(s) 410, 525
MaeI CTAG 2 cut(s) 633, 687
MaeII ACGT 2 cut(s) 699, 804
MaeIII GTNAC 1 cut(s) 391
MalI GATC 1 cut(s) 347
MboI GATC 1 cut(s) 345
MboII GAAGA 7 cut(s) 178, 271, 274, 534, 626, 648, 730
MnlI CCTC 4 cut(s) 88, 93, 299, 375
MroI TCCGGA 1 cut(s) 728
MroNI GCCGGC 1 cut(s) 154
MspA1I CMGCKG 2 cut(s) 675, 773
MspI CCGG 3 cut(s) 18, 155, 729
MspR9I CCNGG 1 cut(s) 492
MvaI CCWGG 1 cut(s) 492
MvnI CGCG 3 cut(s) 35, 48, 663
MwoI GCNNNNNNNGC 5 cut(s) 51, 234, 308, 629, 638
NaeI GCCGGC 1 cut(s) 156
NdeI CATATG 1 cut(s) 565
NdeII GATC 1 cut(s) 345
NgoMIV GCCGGC 1 cut(s) 154
NheI GCTAGC 1 cut(s) 686
NlaIII CATG 6 cut(s) 205, 232, 289, 365, 466, 653
NlaIV GGNNCC 7 cut(s) 81, 99, 460, 489, 593, 670, 705
NmeAIII GCCGAG 1 cut(s) 649
NspI RCATGY 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 631
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 1 cut(s) 215
PflFI GACNNNGTC 1 cut(s) 737
PfoI TCCNGGA 1 cut(s) 490
PinAI ACCGGT 1 cut(s) 17
PmaCI CACGTG 1 cut(s) 700
PmlI CACGTG 1 cut(s) 700
Ppu21I YACGTR 1 cut(s) 700
PpuMI RGGWCCY 1 cut(s) 488
Psp5II RGGWCCY 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 490
PspCI CACGTG 1 cut(s) 700
PspFI CCCAGC 1 cut(s) 474
PspGI CCWGG 1 cut(s) 490
PspN4I GGNNCC 7 cut(s) 81, 99, 460, 489, 593, 670, 705
PspPI GGNCC 4 cut(s) 222, 459, 488, 704
PspPPI RGGWCCY 1 cut(s) 488
PstI CTGCAG 1 cut(s) 772
PstNI CAGNNNCTG 2 cut(s) 395, 782
PsyI GACNNNGTC 1 cut(s) 737
RsaI GTAC 2 cut(s) 295, 593
RsaNI GTAC 2 cut(s) 294, 592
SapI GCTCTTC 1 cut(s) 631
Sau3AI GATC 1 cut(s) 345
Sau96I GGNCC 4 cut(s) 222, 459, 488, 704
ScrFI CCNGG 1 cut(s) 492
SfaNI GCATC 2 cut(s) 410, 525
SfcI CTRYAG 3 cut(s) 609, 754, 768
SinI GGWCC 3 cut(s) 459, 488, 704
SsiI CCGC 9 cut(s) 46, 209, 234, 379, 538, 661, 675, 678, 773
SspMI CTAG 2 cut(s) 633, 687
StyD4I CCNGG 1 cut(s) 490
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 512
TaiI ACGT 2 cut(s) 702, 807
TaqI TCGA 3 cut(s) 24, 66, 93
TauI GCSGC 5 cut(s) 48, 212, 237, 678, 776
TfiI GAWTC 1 cut(s) 215
TscAI CASTG 2 cut(s) 61, 517
TseI GCWGC 5 cut(s) 311, 358, 409, 620, 770
TspDTI ATGAA 2 cut(s) 17, 172
TspGWI ACGGA 2 cut(s) 75, 726
TspRI CASTG 2 cut(s) 61, 517
Tth111I GACNNNGTC 1 cut(s) 737
VpaK11BI GGWCC 3 cut(s) 459, 488, 704
XceI RCATGY 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 693
XspI CTAG 2 cut(s) 633, 687
ZraI GACGTC 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.