RchiOBHm_Chr4g0430701

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000613
Physical Location & Seq
Reverse (-)
55147712 .. 55150995
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39942

Sequence Viewer

Length: 807 bp
ATGAAGTTCGACTTCAGCAGTCTTCAAACCACTGCGCCGTTGCTCGGCGACAGCGGTGGGTTTTCAGCTGCGCCGTCGTTCGAGGTCCCTGTTACTGATGACTTTGATGGGTTTCAGAAAGAGGCTATCCAGATGGTGAAGCCGGCCAAGGGGACTACAACCCTTGCATTTATTTTCAAGGATGGTGTTATGGTGGCGGCTGATTCTCGAGCTAGCATGGGAGGATATATATCGTCTCAGTCTGTGAAGAAGATTATCGAAATCAATCCTTACATGCTTGGAACTATGGCTGGTGGAGCTGCTGACTGTCAGTTTTGGCATAGGAATTTGGGGATTAAGTGCAGACTTCACGAATTGGCAAACAAGCGCAGAATTTCAGTTACAGGTGCATCCAAGCTCTTGGCCAACATTCTGTACTCTTACCGTGGCATGGGTCTGTCTGTTGGGACCATGATAGCTGGGTGGGATGAAACGGGTCCTGGACTTTATTATGTGGACAGTGAGGGAGGAAGGTTGAAAGGGATGCGGTTTTCAGTTGGATCTGGTTCACCATATGCTTATGGTGTCCTGGATAATGGGTACCGCTATGATATGTCCGTTGAAGAAGCTGCTGAGTTAGCTAGGAGAGCCATATATCATGCAACATTCCGTGATGGAGCTAGTGGTGGAGTTGCCAGCGTTTACCATGTCGGTCCAAATGGGTGGAAAAAGCTATCTGGAGATGATGTCGGGGAACTTCACTACACATACTATCCTGTAACCCCAGAGACCGTGGAACAAGAAATGGTGGAGGTAGGTGCAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

268

Amino Acids

28.86

Weight (kDa)

5.76

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 50 - 230 6.3e-53 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 579
AccB1I GGYRCC 1 cut(s) 579
AciI CCGC 4 cut(s) 54, 197, 526, 583
AclWI GGATC 1 cut(s) 547
AcoI YGGCCR 2 cut(s) 144, 402
AcsI RAATTY 2 cut(s) 325, 372
AfaI GTAC 2 cut(s) 416, 581
AfiI CCNNNNNNNGG 3 cut(s) 44, 149, 430
AgsI TTSAA 4 cut(s) 26, 178, 517, 602
AjnI CCWGG 2 cut(s) 478, 567
AluBI AGCT 9 cut(s) 68, 212, 299, 397, 458, 608, 620, 659, 712
AluI AGCT 9 cut(s) 68, 212, 299, 397, 458, 608, 620, 659, 712
Alw26I GTCTC 2 cut(s) 240, 761
AlwI GGATC 1 cut(s) 547
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 2 cut(s) 144, 402
ApeKI GCWGC 4 cut(s) 68, 299, 608, 800
ApoI RAATTY 2 cut(s) 325, 372
Asp718I GGTACC 1 cut(s) 579
AspLEI GCGC 3 cut(s) 37, 73, 369
AspS9I GGNCC 4 cut(s) 85, 447, 476, 692
AsuHPI GGTGA 2 cut(s) 148, 540
AsuNHI GCTAGC 1 cut(s) 212
AvaI CYCGRG 1 cut(s) 207
AvaII GGWCC 4 cut(s) 85, 447, 476, 692
BalI TGGCCA 1 cut(s) 404
BanI GGYRCC 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 3 cut(s) 55, 286, 595
BccI CCATC 4 cut(s) 101, 127, 176, 647
BceAI ACGGC 2 cut(s) 22, 58
BciT130I CCWGG 2 cut(s) 480, 569
BcoDI GTCTC 2 cut(s) 240, 761
BfaI CTAG 3 cut(s) 213, 621, 660
BisI GCNGC 5 cut(s) 69, 198, 300, 609, 801
BlsI GCNGC 5 cut(s) 70, 199, 301, 610, 802
Bme1390I CCNGG 2 cut(s) 480, 569
Bme18I GGWCC 4 cut(s) 85, 447, 476, 692
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 4 cut(s) 85, 447, 476, 692
BmiI GGNNCC 4 cut(s) 87, 448, 477, 581
BmrFI CCNGG 2 cut(s) 480, 569
BmsI GCATC 2 cut(s) 398, 513
BmtI GCTAGC 1 cut(s) 216
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 738
BsaBI GATNNNNATC 1 cut(s) 229
BsaI GGTCTC 1 cut(s) 761
BsaJI CCNNGG 3 cut(s) 147, 424, 771
BsaXI ACNNNNNCTCC 2 cut(s) 711, 741
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 149, 430
Bse118I RCCGGY 1 cut(s) 142
Bse8I GATNNNNATC 1 cut(s) 229
BseBI CCWGG 2 cut(s) 480, 569
BseDI CCNNGG 3 cut(s) 147, 424, 771
BseGI GGATG 4 cut(s) 187, 389, 472, 528
BseJI GATNNNNATC 1 cut(s) 229
BseLI CCNNNNNNNGG 3 cut(s) 44, 149, 430
BseMII CTCAG 2 cut(s) 251, 603
BseXI GCAGC 3 cut(s) 55, 286, 595
BseYI CCCAGC 1 cut(s) 458
BsgI GTGCAG 1 cut(s) 361
BshFI GGCC 2 cut(s) 146, 404
BshNI GGYRCC 1 cut(s) 579
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 1 cut(s) 143
BslFI GGGAC 3 cut(s) 71, 166, 460
BslI CCNNNNNNNGG 3 cut(s) 44, 149, 430
BsmAI GTCTC 2 cut(s) 240, 761
BsmBI CGTCTC 1 cut(s) 240
BsmFI GGGAC 3 cut(s) 71, 166, 460
BsnI GGCC 2 cut(s) 146, 404
Bso31I GGTCTC 1 cut(s) 761
BsoBI CYCGRG 1 cut(s) 207
Bsp143I GATC 1 cut(s) 539
BspACI CCGC 4 cut(s) 54, 197, 526, 583
BspANI GGCC 2 cut(s) 146, 404
BspCNI CTCAG 2 cut(s) 250, 604
BspLI GGNNCC 4 cut(s) 87, 448, 477, 581
BspOI GCTAGC 1 cut(s) 216
BspPI GGATC 1 cut(s) 547
BspT107I GGYRCC 1 cut(s) 579
BspTNI GGTCTC 1 cut(s) 761
BsrFI RCCGGY 1 cut(s) 142
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 3 cut(s) 147, 424, 771
BssMI GATC 1 cut(s) 539
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 2 cut(s) 480, 569
Bst4CI ACNGT 4 cut(s) 308, 425, 500, 772
BstC8I GCNNGC 3 cut(s) 144, 214, 676
BstDEI CTNAG 2 cut(s) 237, 612
BstDSI CCRYGG 2 cut(s) 424, 771
BstF5I GGATG 4 cut(s) 187, 389, 472, 528
BstHHI GCGC 3 cut(s) 37, 73, 369
BstKTI GATC 1 cut(s) 542
BstMAI GTCTC 2 cut(s) 240, 761
BstMBI GATC 1 cut(s) 539
BstMWI GCNNNNNNNGC 3 cut(s) 296, 617, 626
BstNI CCWGG 2 cut(s) 480, 569
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 478, 567
BstV1I GCAGC 3 cut(s) 55, 286, 595
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 539
BstXI CCANNNNNNTGG 2 cut(s) 400, 702
BstYI RGATCY 1 cut(s) 539
BsuRI GGCC 2 cut(s) 146, 404
BtgI CCRYGG 2 cut(s) 424, 771
BtsCI GGATG 4 cut(s) 187, 389, 472, 528
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 2 cut(s) 30, 505
Cac8I GCNNGC 3 cut(s) 144, 214, 676
CfoI GCGC 3 cut(s) 37, 73, 369
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 4 cut(s) 85, 447, 476, 692
Csp6I GTAC 2 cut(s) 415, 580
CviAII CATG 6 cut(s) 217, 274, 430, 451, 638, 686
CviQI GTAC 2 cut(s) 415, 580
DdeI CTNAG 2 cut(s) 237, 612
DpnI GATC 1 cut(s) 541
DpnII GATC 1 cut(s) 539
EaeI YGGCCR 2 cut(s) 144, 402
Eco130I CCWWGG 1 cut(s) 147
Eco31I GGTCTC 1 cut(s) 761
Eco47I GGWCC 4 cut(s) 85, 447, 476, 692
Eco88I CYCGRG 1 cut(s) 207
EcoO109I RGGNCCY 2 cut(s) 85, 476
EcoRII CCWGG 2 cut(s) 478, 567
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
Esp3I CGTCTC 1 cut(s) 240
FaeI CATG 6 cut(s) 220, 277, 433, 454, 641, 689
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 3 cut(s) 71, 166, 460
FatI CATG 6 cut(s) 216, 273, 429, 450, 637, 685
FauNDI CATATG 1 cut(s) 553
Fnu4HI GCNGC 5 cut(s) 69, 198, 300, 609, 801
FokI GGATG 4 cut(s) 194, 376, 479, 535
Fsp4HI GCNGC 5 cut(s) 69, 198, 300, 609, 801
FspBI CTAG 3 cut(s) 213, 621, 660
GlaI GCGC 3 cut(s) 36, 72, 368
GluI GCNGC 5 cut(s) 69, 198, 300, 609, 801
GsaI CCCAGC 1 cut(s) 462
GsuI CTGGAG 1 cut(s) 738
HaeIII GGCC 2 cut(s) 146, 404
HapII CCGG 1 cut(s) 143
HhaI GCGC 3 cut(s) 37, 73, 369
Hin1II CATG 6 cut(s) 220, 277, 433, 454, 641, 689
Hin6I GCGC 3 cut(s) 35, 71, 367
HinP1I GCGC 3 cut(s) 35, 71, 367
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 143
HphI GGTGA 2 cut(s) 148, 540
Hpy166II GTNNAC 3 cut(s) 496, 548, 682
Hpy188I TCNGA 1 cut(s) 117
Hpy188III TCNNGA 4 cut(s) 130, 207, 350, 717
Hpy8I GTNNAC 3 cut(s) 496, 548, 682
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 504
HpyCH4III ACNGT 4 cut(s) 308, 425, 500, 772
HpyCH4V TGCA 5 cut(s) 167, 342, 389, 641, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 617, 626
HpyF3I CTNAG 2 cut(s) 237, 612
Hsp92II CATG 6 cut(s) 220, 277, 433, 454, 641, 689
HspAI GCGC 3 cut(s) 35, 71, 367
KpnI GGTACC 1 cut(s) 583
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Kzo9I GATC 1 cut(s) 539
LmnI GCTCC 2 cut(s) 296, 656
Lsp1109I GCAGC 3 cut(s) 55, 286, 595
LweI GCATC 2 cut(s) 398, 513
MaeI CTAG 3 cut(s) 213, 621, 660
MaeIII GTNAC 3 cut(s) 91, 379, 757
MalI GATC 1 cut(s) 541
MboI GATC 1 cut(s) 539
MboII GAAGA 4 cut(s) 14, 259, 262, 614
MflI RGATCY 1 cut(s) 539
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 3 cut(s) 325, 353, 372
MluNI TGGCCA 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 6 cut(s) 76, 115, 215, 496, 500, 784
Mox20I TGGCCA 1 cut(s) 404
MroNI GCCGGC 1 cut(s) 142
MscI TGGCCA 1 cut(s) 404
MseI TTAA 1 cut(s) 336
Msp20I TGGCCA 1 cut(s) 404
MspA1I CMGCKG 2 cut(s) 54, 68
MspI CCGG 1 cut(s) 143
MspR9I CCNGG 2 cut(s) 480, 569
MvaI CCWGG 2 cut(s) 480, 569
MwoI GCNNNNNNNGC 3 cut(s) 296, 617, 626
NaeI GCCGGC 1 cut(s) 144
NdeI CATATG 1 cut(s) 553
NdeII GATC 1 cut(s) 539
NgoMIV GCCGGC 1 cut(s) 142
NheI GCTAGC 1 cut(s) 212
NlaIII CATG 6 cut(s) 220, 277, 433, 454, 641, 689
NlaIV GGNNCC 4 cut(s) 87, 448, 477, 581
NmeAIII GCCGAG 1 cut(s) 24
NspI RCATGY 1 cut(s) 277
PaeR7I CTCGAG 1 cut(s) 207
PdiI GCCGGC 1 cut(s) 144
PfeI GAWTC 1 cut(s) 203
PfoI TCCNGGA 2 cut(s) 478, 567
PkrI GCNGC 5 cut(s) 70, 199, 301, 610, 802
PpuMI RGGWCCY 2 cut(s) 85, 476
Psp5II RGGWCCY 2 cut(s) 85, 476
Psp6I CCWGG 2 cut(s) 478, 567
PspFI CCCAGC 1 cut(s) 458
PspGI CCWGG 2 cut(s) 478, 567
PspN4I GGNNCC 4 cut(s) 87, 448, 477, 581
PspPI GGNCC 4 cut(s) 85, 447, 476, 692
PspPPI RGGWCCY 2 cut(s) 85, 476
PsuI RGATCY 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 2 cut(s) 416, 581
RsaNI GTAC 2 cut(s) 415, 580
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 5 cut(s) 69, 198, 300, 609, 801
Sau3AI GATC 1 cut(s) 539
Sau96I GGNCC 4 cut(s) 85, 447, 476, 692
ScrFI CCNGG 2 cut(s) 480, 569
SfaNI GCATC 2 cut(s) 398, 513
Sfr274I CTCGAG 1 cut(s) 207
SinI GGWCC 4 cut(s) 85, 447, 476, 692
SlaI CTCGAG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
Sse9I AATT 3 cut(s) 325, 353, 372
SsiI CCGC 4 cut(s) 54, 197, 526, 583
SspMI CTAG 3 cut(s) 213, 621, 660
StyD4I CCNGG 2 cut(s) 478, 567
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 4 cut(s) 308, 425, 500, 772
TaqI TCGA 4 cut(s) 9, 81, 208, 258
TaqII GACCGA 1 cut(s) 680
TasI AATT 3 cut(s) 325, 353, 372
TatI WGTACW 1 cut(s) 414
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 1 cut(s) 203
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 2 cut(s) 37, 505
TseI GCWGC 4 cut(s) 68, 299, 608, 800
TspDTI ATGAA 2 cut(s) 17, 483
TspGWI ACGGA 2 cut(s) 586, 638
TspRI CASTG 2 cut(s) 37, 505
VpaK11BI GGWCC 4 cut(s) 85, 447, 476, 692
XapI RAATTY 2 cut(s) 325, 372
XceI RCATGY 1 cut(s) 277
XhoI CTCGAG 1 cut(s) 207
XspI CTAG 3 cut(s) 213, 621, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.