Rroxscaffold_1G00061240

threonine-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000613
Physical Location & Seq
Reverse (-)
83433783 .. 83435588
1806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061240.1

Sequence Viewer

Length: 1002 bp
ATGCGTTTGAGAAAACCAATCAAGCTAGAGAAGGGGACAATCACTCTTGCTTTTCGATTCAAGGAAGGCATTATGATTGGGGTTGATTCTAGAATCACGCGGAAACAGACTATAGTTTCAGACAAGAAGCAGAAGTTTTTTGAAATATCATCCAACATATTTTGTACAATGATGGGGAGAGTTCATGTCTGGGAAAACATGGCCCTATATGTGCAGCAAAAGGTAAAGAGCGAAGAACGTTTTCGCTGTGCACCTGCAACAGTTTCATATGCAGCAGAAAAGGCGCTTGAACATTTAGAGTATGAATTCAGTGACTATGAATATATGGAGGATGAAGAGGATTTAATATTTGGCACTATCATCGCTGGATGGCAAAAAACAACGGGCTTGGAAGACACATACTGTGAAAGTTCAATAATACTAGGAACTGGTGCTAAATTTGCTGAGGATGTTATGGACTCGGTTAAGAACTTCTACGACATGTCTAGTGAAGATGCTGCCGACTTTGCCCTAAAAGCACTGTTTATGGCAACCTTTTATGATAAGTGGTGTGGAGGGACTCTACATGTTTATCATGTGCATGAAAATGGATATCAAGAGAAACGCAGCCTCGATGCTTTAGAAGTCTACTCGCGATATTACTACATTTACGACCTTTACACATATGAACCAAAGACGTTTTTCTTGCTGTATTCTACAGATTACCAACCCATTGCTGGCGATGATCTCATAAACCTCGGATCAGATGAAGGGCTAGTGGCAGCTCATCTTGTAGCAAAGAAGCGAGACTTTAACATCCACCGCCTCGTGTTCAGCTCTGAGAATGAGGCTTCAACAGCATACGAAGCTATTGCTAAAGTTGAGCTTAGAGGTGCATCTAGCACACTGAAAAGAGTGGCTAATGGCATTAGCCACTATGCAGCCACTAATGAGAAATCAGTCCCCTCTATTGTAGGTGCCATTGGGGCAATAATAGGCACTGATAAAACATCAGTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0001775 GO:0002376 GO:0002520 GO:0002521 GO:0003674 GO:0003824 GO:0004175 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005789 GO:0005829 GO:0005839 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010498 GO:0010499 GO:0012505 GO:0016020 GO:0016043 GO:0016579 GO:0016787 GO:0019221 GO:0019222 GO:0019538 GO:0019774 GO:0019882 GO:0019941 GO:0022607 GO:0023052 GO:0030097 GO:0030098 GO:0030154 GO:0030162 GO:0030163 GO:0030217 GO:0031597 GO:0031974 GO:0031981 GO:0031984 GO:0032501 GO:0032502 GO:0032991 GO:0034097 GO:0034340 GO:0034515 GO:0034622 GO:0036037 GO:0036211 GO:0042110 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043248 GO:0043374 GO:0043412 GO:0043632 GO:0043687 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045087 GO:0045321 GO:0045444 GO:0046631 GO:0046632 GO:0046649 GO:0048513 GO:0048534 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051246 GO:0051336 GO:0051603 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060337 GO:0065003 GO:0065007 GO:0065009 GO:0070011 GO:0070013 GO:0070646 GO:0070647 GO:0070887 GO:0071310 GO:0071345 GO:0071357 GO:0071704 GO:0071840 GO:0080090 GO:0080129 GO:0098827 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369 GO:1990111
KEGG Pathways
Metabolic & Signaling
ko02010 ko03050 ko04145 ko04612 ko05168 ko05340 map02010 map03050 map04145 map04612 map05168 map05340
Pfam Domains
Protein Families
Proteasome

Protein Analysis

333

Amino Acids

37.79

Weight (kDa)

5.34

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 10 - 193 1.1e-18 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 262
Acc36I ACCTGC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 956
AccI GTMKAC 1 cut(s) 627
AccII CGCG 2 cut(s) 100, 634
AciI CCGC 2 cut(s) 100, 802
AclI AACGTT 1 cut(s) 238
AclWI GGATC 1 cut(s) 748
AcsI RAATTY 2 cut(s) 305, 437
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 716
AflIII ACRYGT 2 cut(s) 480, 565
AgsI TTSAA 5 cut(s) 61, 143, 290, 414, 834
AluBI AGCT 5 cut(s) 25, 764, 816, 848, 865
AluI AGCT 5 cut(s) 25, 764, 816, 848, 865
Alw21I GWGCWC 1 cut(s) 253
Alw26I GTCTC 1 cut(s) 780
Alw44I GTGCAC 1 cut(s) 249
AlwI GGATC 1 cut(s) 748
AoxI GGCC 1 cut(s) 201
ApaLI GTGCAC 1 cut(s) 249
ApeKI GCWGC 6 cut(s) 214, 272, 497, 606, 761, 920
ApoI RAATTY 2 cut(s) 305, 437
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 1 cut(s) 202
BaeGI GKGCMC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 956
BauI CACGAG 1 cut(s) 806
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 253
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 6 cut(s) 226, 284, 484, 618, 773, 932
BccI CCATC 2 cut(s) 166, 363
BcgI CGANNNNNNTGC 4 cut(s) 343, 377, 833, 867
BcoDI GTCTC 1 cut(s) 780
BfaI CTAG 6 cut(s) 26, 90, 422, 486, 755, 879
BfmI CTRYAG 2 cut(s) 111, 696
BfoI RGCGCY 1 cut(s) 287
BfuAI ACCTGC 1 cut(s) 262
BglI GCCNNNNNGGC 1 cut(s) 965
BisI GCNGC 6 cut(s) 215, 273, 498, 607, 762, 921
BlsI GCNGC 6 cut(s) 216, 274, 499, 608, 763, 922
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 958
BmsI GCATC 3 cut(s) 484, 604, 884
BpiI GAAGAC 1 cut(s) 399
Bpu10I CCTNAGC 1 cut(s) 444
BsaJI CCNNGG 1 cut(s) 736
Bsc4I CCNNNNNNNGG 1 cut(s) 716
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 711
BseDI CCNNGG 1 cut(s) 736
BseGI GGATG 5 cut(s) 149, 337, 374, 454, 795
BseLI CCNNNNNNNGG 1 cut(s) 716
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 2 cut(s) 435, 810
BseNI ACTGG 1 cut(s) 433
BseSI GKGCMC 1 cut(s) 253
BseXI GCAGC 6 cut(s) 226, 284, 484, 618, 773, 932
BsgI GTGCAG 1 cut(s) 233
Bsh1236I CGCG 2 cut(s) 100, 634
BshFI GGCC 1 cut(s) 203
BshNI GGYRCC 1 cut(s) 956
BsiHKAI GWGCWC 1 cut(s) 253
BslFI GGGAC 3 cut(s) 49, 571, 926
BslI CCNNNNNNNGG 1 cut(s) 716
BsmAI GTCTC 1 cut(s) 780
BsmFI GGGAC 3 cut(s) 49, 571, 926
BsnI GGCC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 253
Bsp1407I TGTACA 1 cut(s) 164
Bsp143I GATC 2 cut(s) 724, 740
Bsp68I TCGCGA 1 cut(s) 634
BspACI CCGC 2 cut(s) 100, 802
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 2 cut(s) 436, 811
BspFNI CGCG 2 cut(s) 100, 634
BspLI GGNNCC 1 cut(s) 958
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 1 cut(s) 748
BspT107I GGYRCC 1 cut(s) 956
BsrDI GCAATG 1 cut(s) 711
BsrGI TGTACA 1 cut(s) 164
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 1 cut(s) 736
BssMI GATC 2 cut(s) 724, 740
BssSI CACGAG 1 cut(s) 806
Bst2BI CACGAG 1 cut(s) 806
Bst4CI ACNGT 3 cut(s) 262, 404, 522
Bst6I CTCTTC 1 cut(s) 330
BstAUI TGTACA 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 718
BstDEI CTNAG 3 cut(s) 444, 819, 866
BstF5I GGATG 5 cut(s) 149, 337, 374, 454, 795
BstFNI CGCG 2 cut(s) 100, 634
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 2 cut(s) 727, 743
BstMAI GTCTC 1 cut(s) 780
BstMBI GATC 2 cut(s) 724, 740
BstMWI GCNNNNNNNGC 7 cut(s) 281, 440, 506, 515, 836, 845, 965
BstNSI RCATGY 2 cut(s) 484, 569
BstSFI CTRYAG 2 cut(s) 111, 696
BstSLI GKGCMC 1 cut(s) 253
BstUI CGCG 2 cut(s) 100, 634
BstV1I GCAGC 6 cut(s) 226, 284, 484, 618, 773, 932
BstV2I GAAGAC 1 cut(s) 399
BsuRI GGCC 1 cut(s) 203
BtgZI GCGATG 2 cut(s) 346, 735
BtsCI GGATG 5 cut(s) 149, 337, 374, 454, 795
BtsIMutI CAGTG 5 cut(s) 316, 518, 884, 978, 999
BtuMI TCGCGA 1 cut(s) 634
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 1 cut(s) 718
CfoI GCGC 1 cut(s) 286
Cfr13I GGNCC 1 cut(s) 202
Csp6I GTAC 1 cut(s) 165
CviAII CATG 6 cut(s) 185, 199, 481, 566, 575, 581
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 3 cut(s) 444, 819, 866
DpnI GATC 2 cut(s) 726, 742
DpnII GATC 2 cut(s) 724, 740
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco32I GATATC 1 cut(s) 593
EcoRI GAATTC 1 cut(s) 305
EcoRV GATATC 1 cut(s) 593
FaeI CATG 6 cut(s) 188, 202, 484, 569, 578, 584
FalI AAGNNNNNCTT 4 cut(s) 773, 805, 849, 881
FaqI GGGAC 3 cut(s) 49, 571, 926
FatI CATG 6 cut(s) 184, 198, 480, 565, 574, 580
FauNDI CATATG 2 cut(s) 268, 664
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 6 cut(s) 215, 273, 498, 607, 762, 921
FokI GGATG 5 cut(s) 136, 344, 381, 461, 782
Fsp4HI GCNGC 6 cut(s) 215, 273, 498, 607, 762, 921
FspBI CTAG 6 cut(s) 26, 90, 422, 486, 755, 879
GlaI GCGC 1 cut(s) 285
GluI GCNGC 6 cut(s) 215, 273, 498, 607, 762, 921
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 1 cut(s) 203
HhaI GCGC 1 cut(s) 286
Hin1II CATG 6 cut(s) 188, 202, 484, 569, 578, 584
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HinfI GANTC 5 cut(s) 57, 86, 93, 458, 559
Hpy166II GTNNAC 2 cut(s) 251, 628
Hpy188I TCNGA 4 cut(s) 121, 740, 745, 820
Hpy188III TCNNGA 3 cut(s) 90, 596, 633
Hpy8I GTNNAC 2 cut(s) 251, 628
HpyAV CCTTC 3 cut(s) 25, 59, 743
HpyCH4III ACNGT 3 cut(s) 262, 404, 522
HpyCH4IV ACGT 2 cut(s) 238, 677
HpyCH4V TGCA 7 cut(s) 214, 251, 257, 272, 580, 875, 920
HpyF10VI GCNNNNNNNGC 7 cut(s) 281, 440, 506, 515, 836, 845, 965
HpyF3I CTNAG 3 cut(s) 444, 819, 866
HpySE526I ACGT 2 cut(s) 238, 677
Hsp92II CATG 6 cut(s) 188, 202, 484, 569, 578, 584
HspAI GCGC 1 cut(s) 284
Kzo9I GATC 2 cut(s) 724, 740
LpnPI CCDG 5 cut(s) 175, 267, 351, 414, 702
Lsp1109I GCAGC 6 cut(s) 226, 284, 484, 618, 773, 932
LweI GCATC 3 cut(s) 484, 604, 884
MaeI CTAG 6 cut(s) 26, 90, 422, 486, 755, 879
MaeII ACGT 2 cut(s) 238, 677
MaeIII GTNAC 1 cut(s) 311
MalI GATC 2 cut(s) 726, 742
MboI GATC 2 cut(s) 724, 740
MboII GAAGA 4 cut(s) 245, 347, 404, 503
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 2 cut(s) 305, 437
MlyI GAGTC 2 cut(s) 452, 553
MmeI TCCRAC 1 cut(s) 177
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 3 cut(s) 344, 465, 792
MslI CAYNNNNRTG 2 cut(s) 579, 585
MvnI CGCG 2 cut(s) 100, 634
MwoI GCNNNNNNNGC 7 cut(s) 281, 440, 506, 515, 836, 845, 965
NdeI CATATG 2 cut(s) 268, 664
NdeII GATC 2 cut(s) 724, 740
NlaIII CATG 6 cut(s) 188, 202, 484, 569, 578, 584
NlaIV GGNNCC 1 cut(s) 958
NmuCI GTSAC 1 cut(s) 311
NruI TCGCGA 1 cut(s) 634
NspI RCATGY 2 cut(s) 484, 569
PaqCI CACCTGC 1 cut(s) 262
PciI ACATGT 2 cut(s) 480, 565
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 3 cut(s) 57, 86, 93
PkrI GCNGC 6 cut(s) 216, 274, 499, 608, 763, 922
PleI GAGTC 2 cut(s) 452, 553
PpsI GAGTC 2 cut(s) 452, 553
PscI ACATGT 2 cut(s) 480, 565
Psp1406I AACGTT 1 cut(s) 238
PspN4I GGNNCC 1 cut(s) 958
PspPI GGNCC 1 cut(s) 202
RruI TCGCGA 1 cut(s) 634
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
RseI CAYNNNNRTG 2 cut(s) 579, 585
SaqAI TTAA 3 cut(s) 344, 465, 792
SatI GCNGC 6 cut(s) 215, 273, 498, 607, 762, 921
Sau3AI GATC 2 cut(s) 724, 740
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 2 cut(s) 452, 553
SduI GDGCHC 1 cut(s) 253
SfaNI GCATC 3 cut(s) 484, 604, 884
SfcI CTRYAG 2 cut(s) 111, 696
SmiMI CAYNNNNRTG 2 cut(s) 579, 585
Sse9I AATT 2 cut(s) 305, 437
SsiI CCGC 2 cut(s) 100, 802
SspI AATATT 1 cut(s) 348
SspMI CTAG 6 cut(s) 26, 90, 422, 486, 755, 879
TaaI ACNGT 3 cut(s) 262, 404, 522
TaiI ACGT 2 cut(s) 241, 680
TaqI TCGA 2 cut(s) 55, 612
TasI AATT 2 cut(s) 305, 437
TatI WGTACW 1 cut(s) 164
TfiI GAWTC 3 cut(s) 57, 86, 93
Tru1I TTAA 3 cut(s) 344, 465, 792
Tru9I TTAA 3 cut(s) 344, 465, 792
TscAI CASTG 5 cut(s) 316, 525, 891, 985, 999
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 6 cut(s) 214, 272, 497, 606, 761, 920
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 8 cut(s) 173, 255, 318, 333, 348, 597, 681, 762
TspRI CASTG 5 cut(s) 316, 525, 891, 985, 999
VneI GTGCAC 1 cut(s) 249
XapI RAATTY 2 cut(s) 305, 437
XbaI TCTAGA 1 cut(s) 89
XceI RCATGY 2 cut(s) 484, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 713
XmiI GTMKAC 1 cut(s) 627
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 6 cut(s) 26, 90, 422, 486, 755, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.