RLG00000031094

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000613
Physical Location & Seq
Forward (+)
1963933 .. 1965678
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031094

Sequence Viewer

Length: 1173 bp
ATGGGTTGCAAGGTTTCAAAACATTCTTCGAACTTGTCGCTCAAGGGGACAACCGCTCTTGCTTTTTTCCACAAAGAGGGCATTGCAGCTGCAGTTGACTCCAGGGCTTCGAACGAAGATGATGAGCTTGAAAAAGAGATTGATATTGTAGACGACGAAGTCAAGATGCATCAAATATCTTCTAAAATAATTTCCTTACTTGTGGGGCGACGTAGTGACTGTCGACTGTTGGGCATTCATATACAAAAGAAGTTTCCGCGCTGGGATGAATATGCTGGCAATGAACCTAGAGTTTCAGATGTAGCAGAAACAATTTATAAGTATCTAAATGGTCTTCGTGGCGAGAATAAAAGCTTTGGAATCCTCATCGCGGGATGGGATGCAGAGGGTCACGATCAAATTTATTGTCTGTTTATAAGAGATGATGTTGTTGTGAAACAAAAAGTAACTTCCTCATCATTTGGAGTACTAGGATCTGGGCGTCTCTATGCTGCTACTAGTCTTAAAAGTGAGAACCGGTGTGCCAACTACGACATGTCCACCGAGGATGGTACTAAGTTGGCCCTAGAAGCTTTATTTAATGCAACCGTCCATGATAGGTGCAGTGGTGGACTTTTGCGAGTTTTTCACGTGGATAAATTTGGATGGCGCCCCGAAATTGTCCAAGATCCTTTAGAAGCCTACTCAATATATTACAAACATTATGAAGAAGAGAAAACGCTTTTCTTAGTTGTTTATTCTCCTTCAAAAATCCCCCCTATTCCTGCCCAGAAACTTATACGACACTTCAGGCAGAAAGGTATTACGGTAACAGCAGCCCATTGTGTAGCCCATCGTGTATTGGAGGAGCAGCATTGTGTAGCCAATGGTGTATTGGAGAAGCAGCGGTTATCCTTCCACCGCCTTATATTCAACTCTAGAGTTGAGGCTTCAAATGCATATGCACTCACAGCTGCTCATGGTGAAACTGATTCTCAACTTGTAGAAAAGTTTCCAAATCAATTATTGTTGAAAACTGCGTTTCAAAAAAAATTGTTGTTGAAAACTGTGGAGCCAAGTGTTGAACTCTATTTGAATCGGAGCTCAAAAGATTTGCTCGAGTTCATACGCACCCAATTTGATGGATATTTGTCAACAGAGATGACACAAATGACCGGAGCACTCGGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

391

Amino Acids

43.91

Weight (kDa)

6.93

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 15 - 209 1.9e-16 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 318, 416
AasI GACNNNNNNGTC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 648
AccBSI CCGCTC 1 cut(s) 56
AccI GTMKAC 2 cut(s) 150, 223
AccII CGCG 2 cut(s) 259, 371
AciI CCGC 5 cut(s) 54, 257, 371, 886, 901
AclWI GGATC 2 cut(s) 481, 662
AcsI RAATTY 2 cut(s) 399, 638
AcuI CTGAAG 1 cut(s) 772
AcvI CACGTG 1 cut(s) 631
AcyI GRCGYC 2 cut(s) 481, 649
AfaI GTAC 2 cut(s) 468, 553
AfiI CCNNNNNNNGG 2 cut(s) 76, 370
AflIII ACRYGT 1 cut(s) 534
AgeI ACCGGT 1 cut(s) 516
AhlI ACTAGT 1 cut(s) 497
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 6 cut(s) 89, 127, 354, 572, 953, 1083
AluI AGCT 6 cut(s) 89, 127, 354, 572, 953, 1083
Alw21I GWGCWC 2 cut(s) 1085, 1162
Alw26I GTCTC 1 cut(s) 488
AlwI GGATC 2 cut(s) 481, 662
Ama87I CYCGRG 1 cut(s) 1097
AoxI GGCC 2 cut(s) 561, 1165
ApeKI GCWGC 7 cut(s) 86, 89, 491, 815, 850, 883, 953
ApoI RAATTY 2 cut(s) 399, 638
ArsI GACNNNNNNTTYG 2 cut(s) 391, 423
AsiGI ACCGGT 1 cut(s) 516
Asp700I GAANNNNTTC 1 cut(s) 990
AspLEI GCGC 2 cut(s) 261, 651
AspS9I GGNCC 1 cut(s) 562
AsuHPI GGTGA 1 cut(s) 974
AsuII TTCGAA 2 cut(s) 29, 110
AvaI CYCGRG 1 cut(s) 1097
BanI GGYRCC 1 cut(s) 648
BanII GRGCYC 1 cut(s) 1085
BbrPI CACGTG 1 cut(s) 631
BbsI GAAGAC 1 cut(s) 326
Bbv12I GWGCWC 2 cut(s) 1085, 1162
BbvI GCAGC 7 cut(s) 76, 98, 478, 827, 862, 895, 940
BccI CCATC 5 cut(s) 369, 542, 639, 840, 1115
BciT130I CCWGG 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 488
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 5 cut(s) 288, 470, 498, 566, 918
BfmI CTRYAG 1 cut(s) 90
BfoI RGCGCY 1 cut(s) 652
BisI GCNGC 7 cut(s) 87, 90, 492, 816, 851, 884, 954
BlsI GCNGC 7 cut(s) 88, 91, 493, 817, 852, 885, 955
BmcAI AGTACT 1 cut(s) 468
Bme1390I CCNGG 1 cut(s) 103
BmeT110I CYCGRG 1 cut(s) 1097
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 2 cut(s) 650, 1053
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 3 cut(s) 156, 178, 370
BpiI GAAGAC 1 cut(s) 326
BpmI CTGGAG 1 cut(s) 85
Bpu14I TTCGAA 2 cut(s) 29, 110
BpuEI CTTGAG 1 cut(s) 26
BsaAI YACGTR 1 cut(s) 631
BsaHI GRCGYC 2 cut(s) 481, 649
BsaJI CCNNGG 2 cut(s) 102, 543
BsaWI WCCGGW 2 cut(s) 516, 1154
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 370
Bse118I RCCGGY 1 cut(s) 516
Bse3DI GCAATG 2 cut(s) 81, 286
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 2 cut(s) 102, 543
BseGI GGATG 5 cut(s) 271, 380, 385, 553, 650
BseLI CCNNNNNNNGG 2 cut(s) 76, 370
BseMI GCAATG 2 cut(s) 81, 286
BseRI GAGGAG 1 cut(s) 860
BseXI GCAGC 7 cut(s) 76, 98, 478, 827, 862, 895, 940
BseYI CCCAGC 1 cut(s) 261
BsgI GTGCAG 1 cut(s) 622
Bsh1236I CGCG 2 cut(s) 259, 371
BshFI GGCC 2 cut(s) 563, 1167
BshNI GGYRCC 1 cut(s) 648
BshTI ACCGGT 1 cut(s) 516
BsiHKAI GWGCWC 2 cut(s) 1085, 1162
BsiHKCI CYCGRG 1 cut(s) 1097
BsiSI CCGG 2 cut(s) 517, 1155
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 2 cut(s) 76, 370
BsmAI GTCTC 1 cut(s) 488
BsmBI CGTCTC 1 cut(s) 488
BsmFI GGGAC 1 cut(s) 61
BsmI GAATGC 1 cut(s) 234
BsnI GGCC 2 cut(s) 563, 1167
BsoBI CYCGRG 1 cut(s) 1097
Bsp119I TTCGAA 2 cut(s) 29, 110
Bsp1286I GDGCHC 2 cut(s) 1085, 1162
Bsp143I GATC 3 cut(s) 394, 473, 667
BspACI CCGC 5 cut(s) 54, 257, 371, 886, 901
BspANI GGCC 2 cut(s) 563, 1167
BspFNI CGCG 2 cut(s) 259, 371
BspLI GGNNCC 2 cut(s) 650, 1053
BspMAI CTGCAG 1 cut(s) 94
BspPI GGATC 2 cut(s) 481, 662
BspT104I TTCGAA 2 cut(s) 29, 110
BspT107I GGYRCC 1 cut(s) 648
BsrBI CCGCTC 1 cut(s) 56
BsrDI GCAATG 2 cut(s) 81, 286
BsrFI RCCGGY 1 cut(s) 516
BssAI RCCGGY 1 cut(s) 516
BssECI CCNNGG 2 cut(s) 102, 543
BssMI GATC 3 cut(s) 394, 473, 667
BssNI GRCGYC 2 cut(s) 481, 649
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 5 cut(s) 221, 228, 589, 808, 1048
Bst6I CTCTTC 1 cut(s) 705
BstACI GRCGYC 2 cut(s) 481, 649
BstBAI YACGTR 1 cut(s) 631
BstBI TTCGAA 2 cut(s) 29, 110
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 2 cut(s) 555, 727
BstF5I GGATG 5 cut(s) 271, 380, 385, 553, 650
BstFNI CGCG 2 cut(s) 259, 371
BstH2I RGCGCY 1 cut(s) 652
BstHHI GCGC 2 cut(s) 261, 651
BstKTI GATC 3 cut(s) 397, 476, 670
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 3 cut(s) 394, 473, 667
BstMWI GCNNNNNNNGC 3 cut(s) 569, 935, 950
BstNI CCWGG 1 cut(s) 103
BstNSI RCATGY 1 cut(s) 538
BstSCI CCNGG 1 cut(s) 101
BstSFI CTRYAG 1 cut(s) 90
BstUI CGCG 2 cut(s) 259, 371
BstV1I GCAGC 7 cut(s) 76, 98, 478, 827, 862, 895, 940
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 2 cut(s) 473, 667
BstXI CCANNNNNNTGG 1 cut(s) 1121
BstYI RGATCY 2 cut(s) 473, 667
BsuRI GGCC 2 cut(s) 563, 1167
BtgZI GCGATG 1 cut(s) 352
BtsCI GGATG 5 cut(s) 271, 380, 385, 553, 650
BtsI GCAGTG 1 cut(s) 610
BtsIMutI CAGTG 1 cut(s) 610
Cac8I GCNNGC 1 cut(s) 277
CfoI GCGC 2 cut(s) 261, 651
Cfr10I RCCGGY 1 cut(s) 516
Cfr13I GGNCC 1 cut(s) 562
CseI GACGC 1 cut(s) 470
Csp6I GTAC 2 cut(s) 467, 552
CspAI ACCGGT 1 cut(s) 516
CviAII CATG 3 cut(s) 535, 593, 959
CviQI GTAC 2 cut(s) 467, 552
DdeI CTNAG 2 cut(s) 555, 727
DinI GGCGCC 1 cut(s) 650
DpnI GATC 3 cut(s) 396, 475, 669
DpnII GATC 3 cut(s) 394, 473, 667
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
Eam1104I CTCTTC 1 cut(s) 705
EarI CTCTTC 1 cut(s) 705
Ecl136II GAGCTC 1 cut(s) 1083
Eco24I GRGCYC 1 cut(s) 1085
Eco53kI GAGCTC 1 cut(s) 1083
Eco57I CTGAAG 1 cut(s) 772
Eco72I CACGTG 1 cut(s) 631
Eco88I CYCGRG 1 cut(s) 1097
EcoICRI GAGCTC 1 cut(s) 1083
EcoRII CCWGG 1 cut(s) 101
EcoT22I ATGCAT 2 cut(s) 171, 940
EcoT38I GRGCYC 1 cut(s) 1085
EgeI GGCGCC 1 cut(s) 650
EheI GGCGCC 1 cut(s) 650
Esp3I CGTCTC 1 cut(s) 488
FaeI CATG 3 cut(s) 538, 596, 962
FaqI GGGAC 1 cut(s) 61
FatI CATG 3 cut(s) 534, 592, 958
FauI CCCGC 1 cut(s) 364
FauNDI CATATG 1 cut(s) 940
FblI GTMKAC 2 cut(s) 150, 223
Fnu4HI GCNGC 7 cut(s) 87, 90, 492, 816, 851, 884, 954
FokI GGATG 5 cut(s) 278, 387, 392, 560, 657
FriOI GRGCYC 1 cut(s) 1085
Fsp4HI GCNGC 7 cut(s) 87, 90, 492, 816, 851, 884, 954
FspBI CTAG 5 cut(s) 288, 470, 498, 566, 918
GlaI GCGC 2 cut(s) 260, 650
GluI GCNGC 7 cut(s) 87, 90, 492, 816, 851, 884, 954
GsaI CCCAGC 1 cut(s) 265
GsuI CTGGAG 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 652
HaeIII GGCC 2 cut(s) 563, 1167
HapII CCGG 2 cut(s) 517, 1155
HgaI GACGC 1 cut(s) 470
HhaI GCGC 2 cut(s) 261, 651
Hin1I GRCGYC 2 cut(s) 481, 649
Hin1II CATG 3 cut(s) 538, 596, 962
Hin6I GCGC 2 cut(s) 259, 649
HinP1I GCGC 2 cut(s) 259, 649
HincII GTYRAC 3 cut(s) 97, 224, 1134
HindII GTYRAC 3 cut(s) 97, 224, 1134
HindIII AAGCTT 2 cut(s) 352, 570
HinfI GANTC 4 cut(s) 98, 360, 971, 1075
HpaII CCGG 2 cut(s) 517, 1155
HphI GGTGA 1 cut(s) 974
Hpy166II GTNNAC 6 cut(s) 97, 151, 224, 540, 611, 1134
Hpy188I TCNGA 2 cut(s) 298, 1080
Hpy188III TCNNGA 3 cut(s) 163, 392, 918
Hpy8I GTNNAC 6 cut(s) 97, 151, 224, 540, 611, 1134
Hpy99I CGWCG 2 cut(s) 158, 213
HpyAV CCTTC 2 cut(s) 753, 904
HpyCH4III ACNGT 5 cut(s) 221, 228, 589, 808, 1048
HpyCH4IV ACGT 2 cut(s) 211, 630
HpyCH4V TGCA 9 cut(s) 9, 86, 92, 169, 383, 584, 603, 938, 944
HpyF10VI GCNNNNNNNGC 3 cut(s) 569, 935, 950
HpyF3I CTNAG 2 cut(s) 555, 727
HpySE526I ACGT 2 cut(s) 211, 630
Hsp92I GRCGYC 2 cut(s) 481, 649
Hsp92II CATG 3 cut(s) 538, 596, 962
HspAI GCGC 2 cut(s) 259, 649
KasI GGCGCC 1 cut(s) 648
Kzo9I GATC 3 cut(s) 394, 473, 667
LmnI GCTCC 4 cut(s) 847, 1051, 1080, 1157
Lsp1109I GCAGC 7 cut(s) 76, 98, 478, 827, 862, 895, 940
LweI GCATC 3 cut(s) 156, 178, 370
MaeI CTAG 5 cut(s) 288, 470, 498, 566, 918
MaeII ACGT 2 cut(s) 211, 630
MaeIII GTNAC 4 cut(s) 215, 389, 445, 808
MalI GATC 3 cut(s) 396, 475, 669
MbiI CCGCTC 1 cut(s) 56
MboI GATC 3 cut(s) 394, 473, 667
MboII GAAGA 6 cut(s) 18, 128, 171, 326, 719, 722
MflI RGATCY 2 cut(s) 473, 667
MhlI GDGCHC 2 cut(s) 1085, 1162
MluCI AATT 8 cut(s) 189, 312, 399, 638, 657, 1001, 1031, 1115
Mly113I GGCGCC 1 cut(s) 649
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 7 cut(s) 70, 374, 379, 463, 538, 838, 919
Mph1103I ATGCAT 2 cut(s) 171, 940
MroXI GAANNNNTTC 1 cut(s) 990
MseI TTAA 2 cut(s) 504, 579
MspA1I CMGCKG 3 cut(s) 89, 886, 953
MspI CCGG 2 cut(s) 517, 1155
MspR9I CCNGG 1 cut(s) 103
Mva1269I GAATGC 1 cut(s) 234
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 2 cut(s) 259, 371
MwoI GCNNNNNNNGC 3 cut(s) 569, 935, 950
NarI GGCGCC 1 cut(s) 649
NdeI CATATG 1 cut(s) 940
NdeII GATC 3 cut(s) 394, 473, 667
NlaIII CATG 3 cut(s) 538, 596, 962
NlaIV GGNNCC 2 cut(s) 650, 1053
NmeAIII GCCGAG 1 cut(s) 1143
NmuCI GTSAC 2 cut(s) 215, 389
NsiI ATGCAT 2 cut(s) 171, 940
NspI RCATGY 1 cut(s) 538
NspV TTCGAA 2 cut(s) 29, 110
PaeR7I CTCGAG 1 cut(s) 1097
PciI ACATGT 1 cut(s) 534
PctI GAATGC 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 3 cut(s) 360, 971, 1075
PflFI GACNNNGTC 1 cut(s) 158
PinAI ACCGGT 1 cut(s) 516
PkrI GCNGC 7 cut(s) 88, 91, 493, 817, 852, 885, 955
PleI GAGTC 1 cut(s) 92
PluTI GGCGCC 1 cut(s) 652
PmaCI CACGTG 1 cut(s) 631
PmlI CACGTG 1 cut(s) 631
PpsI GAGTC 1 cut(s) 92
Ppu21I YACGTR 1 cut(s) 631
PscI ACATGT 1 cut(s) 534
PsiI TTATAA 2 cut(s) 318, 416
Psp124BI GAGCTC 1 cut(s) 1085
Psp6I CCWGG 1 cut(s) 101
PspCI CACGTG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 261
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 650, 1053
PspPI GGNCC 1 cut(s) 562
PspXI VCTCGAGB 1 cut(s) 1097
PstI CTGCAG 1 cut(s) 94
PsuI RGATCY 2 cut(s) 473, 667
PsyI GACNNNGTC 1 cut(s) 158
PvuII CAGCTG 2 cut(s) 89, 953
RsaI GTAC 2 cut(s) 468, 553
RsaNI GTAC 2 cut(s) 467, 552
SacI GAGCTC 1 cut(s) 1085
SalI GTCGAC 1 cut(s) 222
SaqAI TTAA 2 cut(s) 504, 579
SatI GCNGC 7 cut(s) 87, 90, 492, 816, 851, 884, 954
Sau3AI GATC 3 cut(s) 394, 473, 667
Sau96I GGNCC 1 cut(s) 562
ScaI AGTACT 1 cut(s) 468
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 103
SduI GDGCHC 2 cut(s) 1085, 1162
SfaNI GCATC 3 cut(s) 156, 178, 370
SfcI CTRYAG 1 cut(s) 90
SfoI GGCGCC 1 cut(s) 650
Sfr274I CTCGAG 1 cut(s) 1097
SfuI TTCGAA 2 cut(s) 29, 110
SlaI CTCGAG 1 cut(s) 1097
SmlI CTYRAG 2 cut(s) 41, 1097
SmoI CTYRAG 2 cut(s) 41, 1097
SpeI ACTAGT 1 cut(s) 497
Sse9I AATT 8 cut(s) 189, 312, 399, 638, 657, 1001, 1031, 1115
SsiI CCGC 5 cut(s) 54, 257, 371, 886, 901
SspDI GGCGCC 1 cut(s) 648
SspMI CTAG 5 cut(s) 288, 470, 498, 566, 918
SstI GAGCTC 1 cut(s) 1085
StyD4I CCNGG 1 cut(s) 101
TaaI ACNGT 5 cut(s) 221, 228, 589, 808, 1048
TaiI ACGT 2 cut(s) 214, 633
TaqI TCGA 4 cut(s) 29, 110, 223, 1098
TasI AATT 8 cut(s) 189, 312, 399, 638, 657, 1001, 1031, 1115
TatI WGTACW 1 cut(s) 466
TfiI GAWTC 3 cut(s) 360, 971, 1075
Tru1I TTAA 2 cut(s) 504, 579
Tru9I TTAA 2 cut(s) 504, 579
TscAI CASTG 1 cut(s) 610
TseFI GTSAC 2 cut(s) 215, 389
TseI GCWGC 7 cut(s) 86, 89, 491, 815, 850, 883, 953
Tsp45I GTSAC 2 cut(s) 215, 389
TspDTI ATGAA 5 cut(s) 227, 282, 297, 720, 1093
TspRI CASTG 1 cut(s) 610
Tth111I GACNNNGTC 1 cut(s) 158
XapI RAATTY 2 cut(s) 399, 638
XbaI TCTAGA 1 cut(s) 917
XceI RCATGY 1 cut(s) 538
XcmI CCANNNNNNNNNTGG 1 cut(s) 871
XhoI CTCGAG 1 cut(s) 1097
XmiI GTMKAC 2 cut(s) 150, 223
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 5 cut(s) 288, 470, 498, 566, 918
ZrmI AGTACT 1 cut(s) 468
Zsp2I ATGCAT 2 cut(s) 171, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.