The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 432 |
| AccB1I | GGYRCC | 1 cut(s) | 95 |
| AccI | GTMKAC | 2 cut(s) | 417, 573 |
| AclI | AACGTT | 1 cut(s) | 751 |
| AcoI | YGGCCR | 1 cut(s) | 557 |
| AcsI | RAATTY | 3 cut(s) | 231, 425, 496 |
| AfaI | GTAC | 4 cut(s) | 5, 227, 620, 848 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 124, 125, 566 |
| AflIII | ACRYGT | 1 cut(s) | 367 |
| AgsI | TTSAA | 5 cut(s) | 134, 238, 536, 649, 808 |
| AjiI | CACGTC | 1 cut(s) | 384 |
| AluBI | AGCT | 2 cut(s) | 698, 792 |
| AluI | AGCT | 2 cut(s) | 698, 792 |
| Alw21I | GWGCWC | 1 cut(s) | 794 |
| Alw26I | GTCTC | 1 cut(s) | 389 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 555 |
| Ama87I | CYCGRG | 1 cut(s) | 392 |
| AoxI | GGCC | 1 cut(s) | 557 |
| ApeKI | GCWGC | 2 cut(s) | 583, 759 |
| ApoI | RAATTY | 3 cut(s) | 231, 425, 496 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 74, 106 |
| AseI | ATTAAT | 1 cut(s) | 837 |
| AspS9I | GGNCC | 2 cut(s) | 82, 207 |
| AsuHPI | GGTGA | 4 cut(s) | 224, 272, 536, 626 |
| AsuII | TTCGAA | 1 cut(s) | 171 |
| AvaI | CYCGRG | 1 cut(s) | 392 |
| AvaII | GGWCC | 2 cut(s) | 82, 207 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 356, 389 |
| BalI | TGGCCA | 1 cut(s) | 559 |
| BanI | GGYRCC | 1 cut(s) | 95 |
| BanII | GRGCYC | 1 cut(s) | 794 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 848 |
| BbsI | GAAGAC | 3 cut(s) | 378, 543, 804 |
| Bbv12I | GWGCWC | 1 cut(s) | 794 |
| BbvI | GCAGC | 2 cut(s) | 595, 771 |
| BccI | CCATC | 2 cut(s) | 521, 780 |
| BciVI | GTATCC | 1 cut(s) | 104 |
| BcoDI | GTCTC | 1 cut(s) | 389 |
| BfaI | CTAG | 4 cut(s) | 203, 279, 444, 590 |
| BfmI | CTRYAG | 1 cut(s) | 720 |
| BfuI | GTATCC | 1 cut(s) | 104 |
| BisI | GCNGC | 2 cut(s) | 584, 760 |
| BlsI | GCNGC | 2 cut(s) | 585, 761 |
| Bme18I | GGWCC | 2 cut(s) | 82, 207 |
| BmeT110I | CYCGRG | 1 cut(s) | 392 |
| BmgBI | CACGTC | 1 cut(s) | 384 |
| BmgT120I | GGNCC | 2 cut(s) | 82, 207 |
| BmiI | GGNNCC | 2 cut(s) | 97, 208 |
| BmsI | GCATC | 4 cut(s) | 41, 211, 439, 820 |
| BpiI | GAAGAC | 3 cut(s) | 378, 543, 804 |
| Bpu14I | TTCGAA | 1 cut(s) | 171 |
| BsaI | GGTCTC | 1 cut(s) | 389 |
| BsaJI | CCNNGG | 1 cut(s) | 736 |
| BsaWI | WCCGGW | 1 cut(s) | 209 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 124, 125, 566 |
| BseDI | CCNNGG | 1 cut(s) | 736 |
| BseGI | GGATG | 5 cut(s) | 61, 223, 543, 791, 835 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 124, 125, 566 |
| BseMII | CTCAG | 3 cut(s) | 255, 562, 807 |
| BseXI | GCAGC | 2 cut(s) | 595, 771 |
| BshFI | GGCC | 1 cut(s) | 559 |
| BshNI | GGYRCC | 1 cut(s) | 95 |
| BsiHKAI | GWGCWC | 1 cut(s) | 794 |
| BsiHKCI | CYCGRG | 1 cut(s) | 392 |
| BsiSI | CCGG | 1 cut(s) | 210 |
| BsiWI | CGTACG | 1 cut(s) | 225 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 124, 125, 566 |
| BsmAI | GTCTC | 1 cut(s) | 389 |
| BsmI | GAATGC | 1 cut(s) | 65 |
| BsnI | GGCC | 1 cut(s) | 559 |
| Bso31I | GGTCTC | 1 cut(s) | 389 |
| BsoBI | CYCGRG | 1 cut(s) | 392 |
| Bsp119I | TTCGAA | 1 cut(s) | 171 |
| Bsp1286I | GDGCHC | 1 cut(s) | 794 |
| BspANI | GGCC | 1 cut(s) | 559 |
| BspCNI | CTCAG | 3 cut(s) | 254, 561, 806 |
| BspLI | GGNNCC | 2 cut(s) | 97, 208 |
| BspQI | GCTCTTC | 1 cut(s) | 705 |
| BspT104I | TTCGAA | 1 cut(s) | 171 |
| BspT107I | GGYRCC | 1 cut(s) | 95 |
| BspTNI | GGTCTC | 1 cut(s) | 389 |
| BssECI | CCNNGG | 1 cut(s) | 736 |
| BssT1I | CCWWGG | 1 cut(s) | 736 |
| Bst4CI | ACNGT | 3 cut(s) | 8, 260, 638 |
| Bst6I | CTCTTC | 1 cut(s) | 705 |
| BstBI | TTCGAA | 1 cut(s) | 171 |
| BstC8I | GCNNGC | 1 cut(s) | 598 |
| BstDEI | CTNAG | 3 cut(s) | 241, 548, 793 |
| BstF5I | GGATG | 5 cut(s) | 61, 223, 543, 791, 835 |
| BstMAI | GTCTC | 1 cut(s) | 389 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 308, 671 |
| BstNSI | RCATGY | 2 cut(s) | 311, 371 |
| BstSFI | CTRYAG | 1 cut(s) | 720 |
| BstV1I | GCAGC | 2 cut(s) | 595, 771 |
| BstV2I | GAAGAC | 3 cut(s) | 378, 543, 804 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 92 |
| BsuI | GTATCC | 1 cut(s) | 104 |
| BsuRI | GGCC | 1 cut(s) | 559 |
| BtrI | CACGTC | 1 cut(s) | 384 |
| BtsCI | GGATG | 5 cut(s) | 61, 223, 543, 791, 835 |
| Cac8I | GCNNGC | 1 cut(s) | 598 |
| CaiI | CAGNNNCTG | 1 cut(s) | 555 |
| Cfr13I | GGNCC | 2 cut(s) | 82, 207 |
| CseI | GACGC | 1 cut(s) | 5 |
| Csp6I | GTAC | 4 cut(s) | 4, 226, 619, 847 |
| CviAII | CATG | 5 cut(s) | 274, 308, 368, 823, 843 |
| CviQI | GTAC | 4 cut(s) | 4, 226, 619, 847 |
| DdeI | CTNAG | 3 cut(s) | 241, 548, 793 |
| DraI | TTTAAA | 1 cut(s) | 748 |
| EaeI | YGGCCR | 1 cut(s) | 557 |
| Eam1104I | CTCTTC | 1 cut(s) | 705 |
| EarI | CTCTTC | 1 cut(s) | 705 |
| Ecl136II | GAGCTC | 1 cut(s) | 792 |
| Eco130I | CCWWGG | 1 cut(s) | 736 |
| Eco24I | GRGCYC | 1 cut(s) | 794 |
| Eco31I | GGTCTC | 1 cut(s) | 389 |
| Eco47I | GGWCC | 2 cut(s) | 82, 207 |
| Eco53kI | GAGCTC | 1 cut(s) | 792 |
| Eco88I | CYCGRG | 1 cut(s) | 392 |
| EcoICRI | GAGCTC | 1 cut(s) | 792 |
| EcoRI | GAATTC | 1 cut(s) | 496 |
| EcoT14I | CCWWGG | 1 cut(s) | 736 |
| EcoT22I | ATGCAT | 1 cut(s) | 275 |
| EcoT38I | GRGCYC | 1 cut(s) | 794 |
| ErhI | CCWWGG | 1 cut(s) | 736 |
| FaeI | CATG | 5 cut(s) | 277, 311, 371, 826, 846 |
| FatI | CATG | 5 cut(s) | 273, 307, 367, 822, 842 |
| FauNDI | CATATG | 1 cut(s) | 269 |
| FblI | GTMKAC | 2 cut(s) | 417, 573 |
| Fnu4HI | GCNGC | 2 cut(s) | 584, 760 |
| FokI | GGATG | 5 cut(s) | 68, 230, 530, 798, 842 |
| FriOI | GRGCYC | 1 cut(s) | 794 |
| Fsp4HI | GCNGC | 2 cut(s) | 584, 760 |
| FspBI | CTAG | 4 cut(s) | 203, 279, 444, 590 |
| GluI | GCNGC | 2 cut(s) | 584, 760 |
| HaeIII | GGCC | 1 cut(s) | 559 |
| HapII | CCGG | 1 cut(s) | 210 |
| HgaI | GACGC | 1 cut(s) | 5 |
| Hin1II | CATG | 5 cut(s) | 277, 311, 371, 826, 846 |
| HinfI | GANTC | 2 cut(s) | 552, 813 |
| HpaII | CCGG | 1 cut(s) | 210 |
| HphI | GGTGA | 4 cut(s) | 224, 272, 536, 626 |
| Hpy166II | GTNNAC | 4 cut(s) | 263, 418, 574, 658 |
| Hpy188I | TCNGA | 3 cut(s) | 21, 551, 796 |
| Hpy188III | TCNNGA | 3 cut(s) | 392, 394, 533 |
| Hpy8I | GTNNAC | 4 cut(s) | 263, 418, 574, 658 |
| Hpy99I | CGWCG | 1 cut(s) | 21 |
| HpyAV | CCTTC | 2 cut(s) | 515, 820 |
| HpyCH4III | ACNGT | 3 cut(s) | 8, 260, 638 |
| HpyCH4IV | ACGT | 5 cut(s) | 89, 286, 383, 709, 751 |
| HpyCH4V | TGCA | 7 cut(s) | 63, 273, 290, 323, 412, 452, 852 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 308, 671 |
| HpyF3I | CTNAG | 3 cut(s) | 241, 548, 793 |
| HpySE526I | ACGT | 5 cut(s) | 89, 286, 383, 709, 751 |
| Hsp92II | CATG | 5 cut(s) | 277, 311, 371, 826, 846 |
| LguI | GCTCTTC | 1 cut(s) | 705 |
| LmnI | GCTCC | 1 cut(s) | 789 |
| LpnPI | CCDG | 6 cut(s) | 195, 223, 316, 330, 541, 741 |
| Lsp1109I | GCAGC | 2 cut(s) | 595, 771 |
| LweI | GCATC | 4 cut(s) | 41, 211, 439, 820 |
| MaeI | CTAG | 4 cut(s) | 203, 279, 444, 590 |
| MaeII | ACGT | 5 cut(s) | 89, 286, 383, 709, 751 |
| MaeIII | GTNAC | 3 cut(s) | 145, 176, 818 |
| MboII | GAAGA | 5 cut(s) | 378, 548, 692, 737, 809 |
| MhlI | GDGCHC | 1 cut(s) | 794 |
| MlsI | TGGCCA | 1 cut(s) | 559 |
| MluCI | AATT | 6 cut(s) | 192, 231, 425, 496, 742, 838 |
| MluNI | TGGCCA | 1 cut(s) | 559 |
| MlyI | GAGTC | 1 cut(s) | 561 |
| MmeI | TCCRAC | 1 cut(s) | 33 |
| MnlI | CCTC | 5 cut(s) | 73, 208, 551, 557, 560 |
| Mox20I | TGGCCA | 1 cut(s) | 559 |
| Mph1103I | ATGCAT | 1 cut(s) | 275 |
| MscI | TGGCCA | 1 cut(s) | 559 |
| MseI | TTAA | 3 cut(s) | 197, 747, 837 |
| MslI | CAYNNNNRTG | 1 cut(s) | 272 |
| Msp20I | TGGCCA | 1 cut(s) | 559 |
| MspI | CCGG | 1 cut(s) | 210 |
| Mva1269I | GAATGC | 1 cut(s) | 65 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 308, 671 |
| NdeI | CATATG | 1 cut(s) | 269 |
| NlaIII | CATG | 5 cut(s) | 277, 311, 371, 826, 846 |
| NlaIV | GGNNCC | 2 cut(s) | 97, 208 |
| NmuCI | GTSAC | 1 cut(s) | 176 |
| NsiI | ATGCAT | 1 cut(s) | 275 |
| NspI | RCATGY | 2 cut(s) | 311, 371 |
| NspV | TTCGAA | 1 cut(s) | 171 |
| PaeR7I | CTCGAG | 1 cut(s) | 392 |
| PciI | ACATGT | 1 cut(s) | 367 |
| PciSI | GCTCTTC | 1 cut(s) | 705 |
| PctI | GAATGC | 1 cut(s) | 65 |
| PfeI | GAWTC | 1 cut(s) | 813 |
| Pfl23II | CGTACG | 1 cut(s) | 225 |
| PkrI | GCNGC | 2 cut(s) | 585, 761 |
| PleI | GAGTC | 1 cut(s) | 560 |
| PpsI | GAGTC | 1 cut(s) | 560 |
| PscI | ACATGT | 1 cut(s) | 367 |
| PshBI | ATTAAT | 1 cut(s) | 837 |
| PsiI | TTATAA | 1 cut(s) | 432 |
| Psp124BI | GAGCTC | 1 cut(s) | 794 |
| Psp1406I | AACGTT | 1 cut(s) | 751 |
| PspLI | CGTACG | 1 cut(s) | 225 |
| PspN4I | GGNNCC | 2 cut(s) | 97, 208 |
| PspPI | GGNCC | 2 cut(s) | 82, 207 |
| PstNI | CAGNNNCTG | 1 cut(s) | 555 |
| RsaI | GTAC | 4 cut(s) | 5, 227, 620, 848 |
| RsaNI | GTAC | 4 cut(s) | 4, 226, 619, 847 |
| RseI | CAYNNNNRTG | 1 cut(s) | 272 |
| SacI | GAGCTC | 1 cut(s) | 794 |
| SapI | GCTCTTC | 1 cut(s) | 705 |
| SaqAI | TTAA | 3 cut(s) | 197, 747, 837 |
| SatI | GCNGC | 2 cut(s) | 584, 760 |
| Sau96I | GGNCC | 2 cut(s) | 82, 207 |
| SchI | GAGTC | 1 cut(s) | 561 |
| SduI | GDGCHC | 1 cut(s) | 794 |
| SfaNI | GCATC | 4 cut(s) | 41, 211, 439, 820 |
| SfcI | CTRYAG | 1 cut(s) | 720 |
| Sfr274I | CTCGAG | 1 cut(s) | 392 |
| SfuI | TTCGAA | 1 cut(s) | 171 |
| SinI | GGWCC | 2 cut(s) | 82, 207 |
| SlaI | CTCGAG | 1 cut(s) | 392 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 272 |
| SmlI | CTYRAG | 1 cut(s) | 392 |
| SmoI | CTYRAG | 1 cut(s) | 392 |
| Sse9I | AATT | 6 cut(s) | 192, 231, 425, 496, 742, 838 |
| SspMI | CTAG | 4 cut(s) | 203, 279, 444, 590 |
| SstI | GAGCTC | 1 cut(s) | 794 |
| StyI | CCWWGG | 1 cut(s) | 736 |
| TaaI | ACNGT | 3 cut(s) | 8, 260, 638 |
| TaiI | ACGT | 5 cut(s) | 92, 289, 386, 712, 754 |
| TaqI | TCGA | 2 cut(s) | 171, 393 |
| TasI | AATT | 6 cut(s) | 192, 231, 425, 496, 742, 838 |
| TatI | WGTACW | 1 cut(s) | 3 |
| TfiI | GAWTC | 1 cut(s) | 813 |
| Tru1I | TTAA | 3 cut(s) | 197, 747, 837 |
| Tru9I | TTAA | 3 cut(s) | 197, 747, 837 |
| TseFI | GTSAC | 1 cut(s) | 176 |
| TseI | GCWGC | 2 cut(s) | 583, 759 |
| Tsp45I | GTSAC | 1 cut(s) | 176 |
| TspDTI | ATGAA | 4 cut(s) | 72, 452, 831, 839 |
| VpaK11BI | GGWCC | 2 cut(s) | 82, 207 |
| VspI | ATTAAT | 1 cut(s) | 837 |
| XapI | RAATTY | 3 cut(s) | 231, 425, 496 |
| XceI | RCATGY | 2 cut(s) | 311, 371 |
| XhoI | CTCGAG | 1 cut(s) | 392 |
| XmiI | GTMKAC | 2 cut(s) | 417, 573 |
| XspI | CTAG | 4 cut(s) | 203, 279, 444, 590 |
| Zsp2I | ATGCAT | 1 cut(s) | 275 |