pycom10g01980

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000613
Physical Location & Seq
Reverse (-)
2096692 .. 2098615
1924 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g01980.5

Sequence Viewer

Length: 666 bp
ATGGTGAAGCCTGCCAAGGGGACAACCACGCTTGCATTCATCTTCAAGGATGGAGTCATGGTTGCTGCCGATTCTCGAGCTTCAATGGGAGGATATATCTCGTCACAATCTGTGAAGAAGATTATTGAAATCAATCCGTACATGCTTGGTACAATGGCGGGAGGAGCTGCTGACTGCCAGTTTTGGCACAGAAATTTGGGGATTAAGTGTCGGCTTCATGAGTTAGCAAATAAGCGCAGAATTTCTGTTACTGGAGCATCAAAGCTGTTGGCAAATATTTTGTACTCTTACCGCGGAATGGGTCTCTCTGTTGGGACCATGATTGCTGGCTGGGACGAAACAGGTCCTGGACTGTATTATGTTGACAGTGAGGGAGGCCGTTTGAAAGGAATGAGGTTTTCTGTTGGTTCGGGCTCGCCTTACGCTTATGGTGTTCTGGATAATGGGTACCGGTATGACATGTCAGTCGAAGAAGCTGCTGAGTTGGCTAGAAGAGCTATCTATCATGCAACATTCCGCGATGGGGCCAGTGGGGGAGTGGCAAGCGTCTATCACGTGGGGCCAAACGGATGGAAGAAGCTGTCCGGAGACGATGTTGGGGAACTTCACTACCACTATTACCCTGTAGTGCCAGGCTCTGTTGAACAAGAAATGGTTGAAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

222

Amino Acids

23.93

Weight (kDa)

8.41

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 464
Acc65I GGTACC 1 cut(s) 447
AccB1I GGYRCC 1 cut(s) 447
AccII CGCG 2 cut(s) 294, 519
AccIII TCCGGA 1 cut(s) 584
AciI CCGC 4 cut(s) 158, 292, 294, 517
AcsI RAATTY 2 cut(s) 193, 240
AcvI CACGTG 1 cut(s) 556
AfaI GTAC 4 cut(s) 140, 151, 284, 449
AfiI CCNNNNNNNGG 3 cut(s) 17, 298, 523
AflIII ACRYGT 1 cut(s) 459
AgeI ACCGGT 1 cut(s) 450
AgsI TTSAA 6 cut(s) 46, 84, 128, 385, 644, 659
AjnI CCWGG 2 cut(s) 346, 631
AluBI AGCT 6 cut(s) 80, 167, 265, 476, 497, 580
AluI AGCT 6 cut(s) 80, 167, 265, 476, 497, 580
Alw26I GTCTC 2 cut(s) 308, 582
AlwNI CAGNNNCTG 2 cut(s) 347, 638
Ama87I CYCGRG 1 cut(s) 75
Aor13HI TCCGGA 1 cut(s) 584
AoxI GGCC 3 cut(s) 376, 525, 560
ApeKI GCWGC 3 cut(s) 65, 167, 476
ApoI RAATTY 2 cut(s) 193, 240
AsiGI ACCGGT 1 cut(s) 450
Asp718I GGTACC 1 cut(s) 447
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 4 cut(s) 315, 344, 525, 560
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 75
AvaII GGWCC 2 cut(s) 315, 344
BanI GGYRCC 1 cut(s) 447
BanII GRGCYC 1 cut(s) 416
BbrPI CACGTG 1 cut(s) 556
BbvI GCAGC 3 cut(s) 52, 154, 463
BccI CCATC 3 cut(s) 44, 515, 564
BceAI ACGGC 1 cut(s) 363
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BciT130I CCWGG 2 cut(s) 348, 633
BcoDI GTCTC 2 cut(s) 308, 582
BfaI CTAG 1 cut(s) 489
BfmI CTRYAG 1 cut(s) 624
BisI GCNGC 3 cut(s) 66, 168, 477
BlsI GCNGC 3 cut(s) 67, 169, 478
Bme1390I CCNGG 2 cut(s) 348, 633
Bme18I GGWCC 2 cut(s) 315, 344
BmeT110I CYCGRG 1 cut(s) 75
BmgT120I GGNCC 4 cut(s) 315, 344, 525, 560
BmiI GGNNCC 4 cut(s) 316, 449, 526, 561
BmrFI CCNGG 2 cut(s) 348, 633
BmsI GCATC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 556
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 2 cut(s) 15, 292
BsaWI WCCGGW 2 cut(s) 450, 584
BsaXI ACNNNNNCTCC 4 cut(s) 45, 75, 579, 609
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 298, 523
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 3 cut(s) 178, 256, 528
BseAI TCCGGA 1 cut(s) 584
BseBI CCWGG 2 cut(s) 348, 633
BseDI CCNNGG 2 cut(s) 15, 292
BseGI GGATG 2 cut(s) 55, 575
BseLI CCNNNNNNNGG 3 cut(s) 17, 298, 523
BseMII CTCAG 1 cut(s) 471
BseNI ACTGG 3 cut(s) 178, 256, 528
BseRI GAGGAG 1 cut(s) 177
BseXI GCAGC 3 cut(s) 52, 154, 463
BseYI CCCAGC 1 cut(s) 330
Bsh1236I CGCG 2 cut(s) 294, 519
BshFI GGCC 3 cut(s) 378, 527, 562
BshNI GGYRCC 1 cut(s) 447
BshTI ACCGGT 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 75
BsiSI CCGG 2 cut(s) 451, 585
BslFI GGGAC 3 cut(s) 34, 328, 347
BslI CCNNNNNNNGG 3 cut(s) 17, 298, 523
BsmAI GTCTC 2 cut(s) 308, 582
BsmBI CGTCTC 1 cut(s) 582
BsmFI GGGAC 3 cut(s) 34, 328, 347
BsmI GAATGC 1 cut(s) 35
BsnI GGCC 3 cut(s) 378, 527, 562
Bso31I GGTCTC 1 cut(s) 308
BsoBI CYCGRG 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 416
Bsp13I TCCGGA 1 cut(s) 584
BspACI CCGC 4 cut(s) 158, 292, 294, 517
BspANI GGCC 3 cut(s) 378, 527, 562
BspCNI CTCAG 1 cut(s) 472
BspEI TCCGGA 1 cut(s) 584
BspFNI CGCG 2 cut(s) 294, 519
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 4 cut(s) 316, 449, 526, 561
BspQI GCTCTTC 1 cut(s) 487
BspT107I GGYRCC 1 cut(s) 447
BspTNI GGTCTC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 450
BsrI ACTGG 3 cut(s) 178, 256, 528
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 2 cut(s) 15, 292
BssT1I CCWWGG 1 cut(s) 15
Bst2UI CCWGG 2 cut(s) 348, 633
Bst4CI ACNGT 2 cut(s) 354, 368
Bst6I CTCTTC 1 cut(s) 487
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 5 cut(s) 12, 33, 328, 416, 544
BstDEI CTNAG 1 cut(s) 480
BstDSI CCRYGG 1 cut(s) 292
BstF5I GGATG 2 cut(s) 55, 575
BstFNI CGCG 2 cut(s) 294, 519
BstHHI GCGC 1 cut(s) 237
BstMAI GTCTC 2 cut(s) 308, 582
BstMWI GCNNNNNNNGC 3 cut(s) 164, 485, 494
BstNI CCWGG 2 cut(s) 348, 633
BstNSI RCATGY 2 cut(s) 145, 463
BstSCI CCNGG 2 cut(s) 346, 631
BstSFI CTRYAG 1 cut(s) 624
BstUI CGCG 2 cut(s) 294, 519
BstV1I GCAGC 3 cut(s) 52, 154, 463
BstXI CCANNNNNNTGG 1 cut(s) 570
BsuRI GGCC 3 cut(s) 378, 527, 562
BtgI CCRYGG 1 cut(s) 292
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 2 cut(s) 55, 575
BtsIMutI CAGTG 2 cut(s) 373, 535
Cac8I GCNNGC 5 cut(s) 12, 33, 328, 416, 544
CaiI CAGNNNCTG 2 cut(s) 347, 638
CciI TCATGA 1 cut(s) 217
CfoI GCGC 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 4 cut(s) 315, 344, 525, 560
Cfr42I CCGCGG 1 cut(s) 295
CseI GACGC 1 cut(s) 535
Csp6I GTAC 4 cut(s) 139, 150, 283, 448
CspAI ACCGGT 1 cut(s) 450
CviAII CATG 6 cut(s) 58, 142, 218, 319, 460, 506
CviQI GTAC 4 cut(s) 139, 150, 283, 448
DdeI CTNAG 1 cut(s) 480
DrdI GACNNNNNNGTC 1 cut(s) 464
DseDI GACNNNNNNGTC 1 cut(s) 464
Eam1104I CTCTTC 1 cut(s) 487
EarI CTCTTC 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 15
Eco24I GRGCYC 1 cut(s) 416
Eco31I GGTCTC 1 cut(s) 308
Eco47I GGWCC 2 cut(s) 315, 344
Eco72I CACGTG 1 cut(s) 556
Eco88I CYCGRG 1 cut(s) 75
EcoO109I RGGNCCY 1 cut(s) 344
EcoRII CCWGG 2 cut(s) 346, 631
EcoT14I CCWWGG 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 416
ErhI CCWWGG 1 cut(s) 15
Esp3I CGTCTC 1 cut(s) 582
FaeI CATG 6 cut(s) 61, 145, 221, 322, 463, 509
FaqI GGGAC 3 cut(s) 34, 328, 347
FatI CATG 6 cut(s) 57, 141, 217, 318, 459, 505
FauI CCCGC 1 cut(s) 151
Fnu4HI GCNGC 3 cut(s) 66, 168, 477
FokI GGATG 2 cut(s) 62, 582
FriOI GRGCYC 1 cut(s) 416
Fsp4HI GCNGC 3 cut(s) 66, 168, 477
FspBI CTAG 1 cut(s) 489
GlaI GCGC 1 cut(s) 236
GluI GCNGC 3 cut(s) 66, 168, 477
GsaI CCCAGC 1 cut(s) 334
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 3 cut(s) 378, 527, 562
HapII CCGG 2 cut(s) 451, 585
HgaI GACGC 1 cut(s) 535
HhaI GCGC 1 cut(s) 237
Hin1II CATG 6 cut(s) 61, 145, 221, 322, 463, 509
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 2 cut(s) 54, 71
HpaII CCGG 2 cut(s) 451, 585
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 1 cut(s) 665
Hpy188III TCNNGA 4 cut(s) 75, 218, 437, 585
Hpy8I GTNNAC 1 cut(s) 364
HpyCH4III ACNGT 2 cut(s) 354, 368
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 2 cut(s) 35, 509
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 485, 494
HpyF3I CTNAG 1 cut(s) 480
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 6 cut(s) 61, 145, 221, 322, 463, 509
HspAI GCGC 1 cut(s) 235
Kpn2I TCCGGA 1 cut(s) 584
KpnI GGTACC 1 cut(s) 451
KspI CCGCGG 1 cut(s) 295
LguI GCTCTTC 1 cut(s) 487
LmnI GCTCC 2 cut(s) 164, 254
Lsp1109I GCAGC 3 cut(s) 52, 154, 463
LweI GCATC 1 cut(s) 266
MaeI CTAG 1 cut(s) 489
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 2 cut(s) 102, 247
MboII GAAGA 6 cut(s) 34, 127, 130, 482, 504, 586
MhlI GDGCHC 1 cut(s) 416
MluCI AATT 2 cut(s) 193, 240
MlyI GAGTC 1 cut(s) 63
MnlI CCTC 5 cut(s) 83, 155, 364, 368, 387
MroI TCCGGA 1 cut(s) 584
MseI TTAA 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 2 cut(s) 451, 585
MspR9I CCNGG 2 cut(s) 348, 633
Mva1269I GAATGC 1 cut(s) 35
MvaI CCWGG 2 cut(s) 348, 633
MvnI CGCG 2 cut(s) 294, 519
MwoI GCNNNNNNNGC 3 cut(s) 164, 485, 494
NlaIII CATG 6 cut(s) 61, 145, 221, 322, 463, 509
NlaIV GGNNCC 4 cut(s) 316, 449, 526, 561
NmuCI GTSAC 1 cut(s) 102
NspI RCATGY 2 cut(s) 145, 463
PaeR7I CTCGAG 1 cut(s) 75
PagI TCATGA 1 cut(s) 217
PciI ACATGT 1 cut(s) 459
PciSI GCTCTTC 1 cut(s) 487
PctI GAATGC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 71
PfoI TCCNGGA 1 cut(s) 346
PinAI ACCGGT 1 cut(s) 450
PkrI GCNGC 3 cut(s) 67, 169, 478
PleI GAGTC 1 cut(s) 62
PmaCI CACGTG 1 cut(s) 556
PmlI CACGTG 1 cut(s) 556
PpsI GAGTC 1 cut(s) 62
Ppu21I YACGTR 1 cut(s) 556
PpuMI RGGWCCY 1 cut(s) 344
PscI ACATGT 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 344
Psp6I CCWGG 2 cut(s) 346, 631
PspCI CACGTG 1 cut(s) 556
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 2 cut(s) 346, 631
PspN4I GGNNCC 4 cut(s) 316, 449, 526, 561
PspPI GGNCC 4 cut(s) 315, 344, 525, 560
PspPPI RGGWCCY 1 cut(s) 344
PstNI CAGNNNCTG 2 cut(s) 347, 638
RsaI GTAC 4 cut(s) 140, 151, 284, 449
RsaNI GTAC 4 cut(s) 139, 150, 283, 448
SacII CCGCGG 1 cut(s) 295
SapI GCTCTTC 1 cut(s) 487
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 3 cut(s) 66, 168, 477
Sau96I GGNCC 4 cut(s) 315, 344, 525, 560
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 2 cut(s) 348, 633
SduI GDGCHC 1 cut(s) 416
SetI ASST 9 cut(s) 82, 169, 267, 346, 398, 478, 499, 558, 582
SfaNI GCATC 1 cut(s) 266
SfcI CTRYAG 1 cut(s) 624
Sfr274I CTCGAG 1 cut(s) 75
Sfr303I CCGCGG 1 cut(s) 295
SgrBI CCGCGG 1 cut(s) 295
SinI GGWCC 2 cut(s) 315, 344
SlaI CTCGAG 1 cut(s) 75
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 2 cut(s) 193, 240
SsiI CCGC 4 cut(s) 158, 292, 294, 517
SspI AATATT 1 cut(s) 277
SspMI CTAG 1 cut(s) 489
StyD4I CCNGG 2 cut(s) 346, 631
StyI CCWWGG 1 cut(s) 15
TaaI ACNGT 2 cut(s) 354, 368
TaiI ACGT 1 cut(s) 558
TaqI TCGA 2 cut(s) 76, 468
TasI AATT 2 cut(s) 193, 240
TatI WGTACW 1 cut(s) 282
TfiI GAWTC 1 cut(s) 71
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 2 cut(s) 373, 535
TseFI GTSAC 1 cut(s) 102
TseI GCWGC 3 cut(s) 65, 167, 476
Tsp45I GTSAC 1 cut(s) 102
TspDTI ATGAA 2 cut(s) 28, 206
TspGWI ACGGA 2 cut(s) 126, 582
TspRI CASTG 2 cut(s) 373, 535
VpaK11BI GGWCC 2 cut(s) 315, 344
XapI RAATTY 2 cut(s) 193, 240
XceI RCATGY 2 cut(s) 145, 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XhoI CTCGAG 1 cut(s) 75
XspI CTAG 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.