The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 347 |
| AccII | CGCG | 1 cut(s) | 339 |
| AciI | CCGC | 2 cut(s) | 678, 778 |
| AclWI | GGATC | 1 cut(s) | 671 |
| AcoI | YGGCCR | 1 cut(s) | 168 |
| AcsI | RAATTY | 4 cut(s) | 329, 715, 749, 947 |
| AfaI | GTAC | 1 cut(s) | 857 |
| AfeI | AGCGCT | 1 cut(s) | 393 |
| AgsI | TTSAA | 5 cut(s) | 18, 431, 629, 703, 797 |
| AluBI | AGCT | 6 cut(s) | 14, 95, 127, 568, 593, 930 |
| AluI | AGCT | 6 cut(s) | 14, 95, 127, 568, 593, 930 |
| Alw21I | GWGCWC | 1 cut(s) | 932 |
| AlwI | GGATC | 1 cut(s) | 671 |
| Aor51HI | AGCGCT | 1 cut(s) | 393 |
| AoxI | GGCC | 1 cut(s) | 168 |
| ApeKI | GCWGC | 2 cut(s) | 464, 565 |
| ApoI | RAATTY | 4 cut(s) | 329, 715, 749, 947 |
| AseI | ATTAAT | 1 cut(s) | 546 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 126, 867 |
| AspLEI | GCGC | 1 cut(s) | 394 |
| AspS9I | GGNCC | 2 cut(s) | 31, 323 |
| AsuC2I | CCSGG | 1 cut(s) | 661 |
| AsuHPI | GGTGA | 1 cut(s) | 770 |
| AvaII | GGWCC | 2 cut(s) | 31, 323 |
| BanII | GRGCYC | 1 cut(s) | 932 |
| Bbv12I | GWGCWC | 1 cut(s) | 932 |
| BbvI | GCAGC | 2 cut(s) | 451, 577 |
| BccI | CCATC | 4 cut(s) | 300, 302, 552, 738 |
| BceAI | ACGGC | 1 cut(s) | 94 |
| BcnI | CCSGG | 1 cut(s) | 661 |
| BfaI | CTAG | 4 cut(s) | 102, 219, 395, 860 |
| BfoI | RGCGCY | 1 cut(s) | 395 |
| BisI | GCNGC | 2 cut(s) | 465, 566 |
| BlsI | GCNGC | 2 cut(s) | 466, 567 |
| Bme1390I | CCNGG | 1 cut(s) | 661 |
| Bme18I | GGWCC | 2 cut(s) | 31, 323 |
| BmgT120I | GGNCC | 2 cut(s) | 31, 323 |
| BmiI | GGNNCC | 2 cut(s) | 292, 324 |
| BmrFI | CCNGG | 1 cut(s) | 661 |
| BmsI | GCATC | 6 cut(s) | 90, 301, 451, 646, 734, 793 |
| BpuEI | CTTGAG | 2 cut(s) | 26, 916 |
| BpuMI | CCSGG | 1 cut(s) | 661 |
| BsaBI | GATNNNNATC | 1 cut(s) | 201 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 814, 844 |
| Bse1I | ACTGG | 1 cut(s) | 726 |
| Bse8I | GATNNNNATC | 1 cut(s) | 201 |
| BseGI | GGATG | 5 cut(s) | 311, 316, 563, 661, 725 |
| BseJI | GATNNNNATC | 1 cut(s) | 201 |
| BseNI | ACTGG | 1 cut(s) | 726 |
| BseRI | GAGGAG | 1 cut(s) | 822 |
| BseXI | GCAGC | 2 cut(s) | 451, 577 |
| Bsh1236I | CGCG | 1 cut(s) | 339 |
| Bsh1285I | CGRYCG | 1 cut(s) | 841 |
| BshFI | GGCC | 1 cut(s) | 170 |
| BsiEI | CGRYCG | 1 cut(s) | 841 |
| BsiHKAI | GWGCWC | 1 cut(s) | 932 |
| BsiSI | CCGG | 1 cut(s) | 660 |
| BslFI | GGGAC | 1 cut(s) | 61 |
| BsmFI | GGGAC | 1 cut(s) | 61 |
| BsmI | GAATGC | 1 cut(s) | 586 |
| BsnI | GGCC | 1 cut(s) | 170 |
| Bsp1286I | GDGCHC | 1 cut(s) | 932 |
| Bsp143I | GATC | 2 cut(s) | 663, 874 |
| BspACI | CCGC | 2 cut(s) | 678, 778 |
| BspANI | GGCC | 1 cut(s) | 170 |
| BspFNI | CGCG | 1 cut(s) | 339 |
| BspHI | TCATGA | 1 cut(s) | 576 |
| BspLI | GGNNCC | 2 cut(s) | 292, 324 |
| BspPI | GGATC | 1 cut(s) | 671 |
| BspQI | GCTCTTC | 1 cut(s) | 423 |
| BsrI | ACTGG | 1 cut(s) | 726 |
| BssMI | GATC | 2 cut(s) | 663, 874 |
| Bst4CI | ACNGT | 5 cut(s) | 620, 839, 867, 913, 985 |
| Bst6I | CTCTTC | 2 cut(s) | 31, 423 |
| BstDEI | CTNAG | 1 cut(s) | 140 |
| BstF5I | GGATG | 5 cut(s) | 311, 316, 563, 661, 725 |
| BstFNI | CGCG | 1 cut(s) | 339 |
| BstH2I | RGCGCY | 1 cut(s) | 395 |
| BstHHI | GCGC | 1 cut(s) | 394 |
| BstKTI | GATC | 2 cut(s) | 666, 877 |
| BstMBI | GATC | 2 cut(s) | 663, 874 |
| BstMCI | CGRYCG | 1 cut(s) | 841 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 391, 473, 590, 812 |
| BstNSI | RCATGY | 1 cut(s) | 713 |
| BstSCI | CCNGG | 1 cut(s) | 659 |
| BstUI | CGCG | 1 cut(s) | 339 |
| BstV1I | GCAGC | 2 cut(s) | 451, 577 |
| BsuRI | GGCC | 1 cut(s) | 170 |
| BtgZI | GCGATG | 1 cut(s) | 320 |
| BtsCI | GGATG | 5 cut(s) | 311, 316, 563, 661, 725 |
| BtsIMutI | CAGTG | 1 cut(s) | 453 |
| CciI | TCATGA | 1 cut(s) | 576 |
| CfoI | GCGC | 1 cut(s) | 394 |
| Cfr13I | GGNCC | 2 cut(s) | 31, 323 |
| Csp6I | GTAC | 1 cut(s) | 856 |
| CviAII | CATG | 5 cut(s) | 85, 228, 413, 577, 710 |
| CviQI | GTAC | 1 cut(s) | 856 |
| DdeI | CTNAG | 1 cut(s) | 140 |
| DpnI | GATC | 2 cut(s) | 665, 876 |
| DpnII | GATC | 2 cut(s) | 663, 874 |
| DraI | TTTAAA | 1 cut(s) | 481 |
| EaeI | YGGCCR | 1 cut(s) | 168 |
| Eam1104I | CTCTTC | 2 cut(s) | 31, 423 |
| EarI | CTCTTC | 2 cut(s) | 31, 423 |
| Ecl136II | GAGCTC | 1 cut(s) | 930 |
| Eco24I | GRGCYC | 1 cut(s) | 932 |
| Eco47I | GGWCC | 2 cut(s) | 31, 323 |
| Eco47III | AGCGCT | 1 cut(s) | 393 |
| Eco53kI | GAGCTC | 1 cut(s) | 930 |
| EcoICRI | GAGCTC | 1 cut(s) | 930 |
| EcoO109I | RGGNCCY | 1 cut(s) | 323 |
| EcoT38I | GRGCYC | 1 cut(s) | 932 |
| FaeI | CATG | 5 cut(s) | 88, 231, 416, 580, 713 |
| FaqI | GGGAC | 1 cut(s) | 61 |
| FatI | CATG | 5 cut(s) | 84, 227, 412, 576, 709 |
| FauNDI | CATATG | 1 cut(s) | 817 |
| Fnu4HI | GCNGC | 2 cut(s) | 465, 566 |
| FokI | GGATG | 5 cut(s) | 318, 323, 570, 668, 712 |
| FriOI | GRGCYC | 1 cut(s) | 932 |
| Fsp4HI | GCNGC | 2 cut(s) | 465, 566 |
| FspBI | CTAG | 4 cut(s) | 102, 219, 395, 860 |
| GlaI | GCGC | 1 cut(s) | 393 |
| GluI | GCNGC | 2 cut(s) | 465, 566 |
| HaeII | RGCGCY | 1 cut(s) | 395 |
| HaeIII | GGCC | 1 cut(s) | 170 |
| HapII | CCGG | 1 cut(s) | 660 |
| HhaI | GCGC | 1 cut(s) | 394 |
| Hin1II | CATG | 5 cut(s) | 88, 231, 416, 580, 713 |
| Hin6I | GCGC | 1 cut(s) | 392 |
| HinP1I | GCGC | 1 cut(s) | 392 |
| HindIII | AAGCTT | 2 cut(s) | 125, 591 |
| HinfI | GANTC | 3 cut(s) | 98, 551, 898 |
| HpaII | CCGG | 1 cut(s) | 660 |
| HphI | GGTGA | 1 cut(s) | 770 |
| Hpy166II | GTNNAC | 1 cut(s) | 453 |
| Hpy188I | TCNGA | 2 cut(s) | 31, 258 |
| Hpy188III | TCNNGA | 5 cut(s) | 266, 326, 555, 577, 872 |
| Hpy8I | GTNNAC | 1 cut(s) | 453 |
| HpyAV | CCTTC | 1 cut(s) | 623 |
| HpyCH4III | ACNGT | 5 cut(s) | 620, 839, 867, 913, 985 |
| HpyCH4V | TGCA | 3 cut(s) | 497, 713, 806 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 391, 473, 590, 812 |
| HpyF3I | CTNAG | 1 cut(s) | 140 |
| Hsp92II | CATG | 5 cut(s) | 88, 231, 416, 580, 713 |
| HspAI | GCGC | 1 cut(s) | 392 |
| Kzo9I | GATC | 2 cut(s) | 663, 874 |
| LguI | GCTCTTC | 1 cut(s) | 423 |
| LmnI | GCTCC | 5 cut(s) | 11, 92, 290, 573, 927 |
| LpnPI | CCDG | 9 cut(s) | 47, 251, 339, 540, 585, 673, 707, 885, 929 |
| Lsp1109I | GCAGC | 2 cut(s) | 451, 577 |
| LweI | GCATC | 6 cut(s) | 90, 301, 451, 646, 734, 793 |
| MaeI | CTAG | 4 cut(s) | 102, 219, 395, 860 |
| MaeIII | GTNAC | 2 cut(s) | 74, 907 |
| MalI | GATC | 2 cut(s) | 665, 876 |
| MboI | GATC | 2 cut(s) | 663, 874 |
| MboII | GAAGA | 4 cut(s) | 18, 171, 410, 809 |
| MhlI | GDGCHC | 1 cut(s) | 932 |
| MluCI | AATT | 8 cut(s) | 54, 144, 189, 329, 715, 749, 922, 947 |
| MmeI | TCCRAC | 3 cut(s) | 70, 207, 283 |
| MnlI | CCTC | 6 cut(s) | 368, 392, 749, 752, 843, 862 |
| MroXI | GAANNNNTTC | 2 cut(s) | 126, 867 |
| MseI | TTAA | 4 cut(s) | 480, 492, 546, 994 |
| MslI | CAYNNNNRTG | 2 cut(s) | 519, 546 |
| MspI | CCGG | 1 cut(s) | 660 |
| MspR9I | CCNGG | 1 cut(s) | 661 |
| Mva1269I | GAATGC | 1 cut(s) | 586 |
| MvnI | CGCG | 1 cut(s) | 339 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 391, 473, 590, 812 |
| NciI | CCSGG | 1 cut(s) | 661 |
| NdeI | CATATG | 1 cut(s) | 817 |
| NdeII | GATC | 2 cut(s) | 663, 874 |
| NlaIII | CATG | 5 cut(s) | 88, 231, 416, 580, 713 |
| NlaIV | GGNNCC | 2 cut(s) | 292, 324 |
| NmuCI | GTSAC | 1 cut(s) | 74 |
| NspI | RCATGY | 1 cut(s) | 713 |
| PagI | TCATGA | 1 cut(s) | 576 |
| PciSI | GCTCTTC | 1 cut(s) | 423 |
| PctI | GAATGC | 1 cut(s) | 586 |
| PdmI | GAANNNNTTC | 2 cut(s) | 126, 867 |
| PfeI | GAWTC | 3 cut(s) | 98, 551, 898 |
| PkrI | GCNGC | 2 cut(s) | 466, 567 |
| PpuMI | RGGWCCY | 1 cut(s) | 323 |
| PshBI | ATTAAT | 1 cut(s) | 546 |
| PsiI | TTATAA | 1 cut(s) | 347 |
| Psp124BI | GAGCTC | 1 cut(s) | 932 |
| Psp5II | RGGWCCY | 1 cut(s) | 323 |
| PspN4I | GGNNCC | 2 cut(s) | 292, 324 |
| PspPI | GGNCC | 2 cut(s) | 31, 323 |
| PspPPI | RGGWCCY | 1 cut(s) | 323 |
| RsaI | GTAC | 1 cut(s) | 857 |
| RsaNI | GTAC | 1 cut(s) | 856 |
| RseI | CAYNNNNRTG | 2 cut(s) | 519, 546 |
| SacI | GAGCTC | 1 cut(s) | 932 |
| SapI | GCTCTTC | 1 cut(s) | 423 |
| SaqAI | TTAA | 4 cut(s) | 480, 492, 546, 994 |
| SatI | GCNGC | 2 cut(s) | 465, 566 |
| Sau3AI | GATC | 2 cut(s) | 663, 874 |
| Sau96I | GGNCC | 2 cut(s) | 31, 323 |
| ScrFI | CCNGG | 1 cut(s) | 661 |
| SduI | GDGCHC | 1 cut(s) | 932 |
| SfaNI | GCATC | 6 cut(s) | 90, 301, 451, 646, 734, 793 |
| SinI | GGWCC | 2 cut(s) | 31, 323 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 519, 546 |
| SmlI | CTYRAG | 2 cut(s) | 41, 931 |
| SmoI | CTYRAG | 2 cut(s) | 41, 931 |
| Sse9I | AATT | 8 cut(s) | 54, 144, 189, 329, 715, 749, 922, 947 |
| SsiI | CCGC | 2 cut(s) | 678, 778 |
| SspMI | CTAG | 4 cut(s) | 102, 219, 395, 860 |
| SstI | GAGCTC | 1 cut(s) | 932 |
| StyD4I | CCNGG | 1 cut(s) | 659 |
| TaaI | ACNGT | 5 cut(s) | 620, 839, 867, 913, 985 |
| TaqI | TCGA | 4 cut(s) | 438, 666, 789, 841 |
| TasI | AATT | 8 cut(s) | 54, 144, 189, 329, 715, 749, 922, 947 |
| TfiI | GAWTC | 3 cut(s) | 98, 551, 898 |
| Tru1I | TTAA | 4 cut(s) | 480, 492, 546, 994 |
| Tru9I | TTAA | 4 cut(s) | 480, 492, 546, 994 |
| TscAI | CASTG | 1 cut(s) | 460 |
| TseFI | GTSAC | 1 cut(s) | 74 |
| TseI | GCWGC | 2 cut(s) | 464, 565 |
| Tsp45I | GTSAC | 1 cut(s) | 74 |
| TspDTI | ATGAA | 9 cut(s) | 129, 135, 213, 216, 323, 564, 593, 834, 975 |
| TspGWI | ACGGA | 2 cut(s) | 62, 646 |
| TspRI | CASTG | 1 cut(s) | 460 |
| VpaK11BI | GGWCC | 2 cut(s) | 31, 323 |
| VspI | ATTAAT | 1 cut(s) | 546 |
| XapI | RAATTY | 4 cut(s) | 329, 715, 749, 947 |
| XceI | RCATGY | 1 cut(s) | 713 |
| XmnI | GAANNNNTTC | 2 cut(s) | 126, 867 |
| XspI | CTAG | 4 cut(s) | 102, 219, 395, 860 |