Rw4G026180

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000613
Physical Location & Seq
Reverse (-)
52348544 .. 52351740
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026180.1

Sequence Viewer

Length: 810 bp
ATGAAGTTCGACTTCAGCAGTCTTCAAACCACTGCGCCGTTGCTCGGCGACAGCAGCGGTGGGTTTTCAGCTGCGCCGTCGTTCGAGGTCCCTGTTACTGATGACTTTGATGGGTTTCAGAAAGAGGCTATCCAGATGGTGAAGCCGGCCAAGGGGACTACAACCCTTGCATTTATTTTCAAGGATGGTGTTATGGTGGCGGCTGATTCTCGAGCTAGCATGGGAGGATATATATCGTCTCAGTCTGTGAAGAAGATTATCGAAATCAATCCTTACATGCTTGGAACTATGGCTGGTGGAGCTGCTGACTGTCAGTTTTGGCATAGGAATTTGGGGATTAAGTGCAGACTTCACGAATTGGCAAACAAGCGCAGAATTTCAGTTACAGGTGCATCCAAGCTCTTGGCCAACATTCTGTACTCTTACCGTGGAATGGGTCTGTCTGTTGGGACCATGATAGCTGGGTGGGATGAAACGGGTCCTGGACTTTATTATGTGGACAGTGAGGGAGGAAGGTTGAAAGGGATGCGGTTTTCAGTTGGATCTGGTTCACCATATGCTTATGGTGTCCTGGATAATGGGTACCGCTATGATATGTCAGTTGAAGAAGCTGCTGAGTTAGCTAGGAGAGCCATATATCATGCAACATTCCGTGATGGAGCTAGTGGTGGAGTTGCCAGTGTTTACCATGTCGGTCCAAATGGGTGGAAAAAGCTATCTGGAGATGATGTCGGGGAACTTCACTACACATACTATCCTGTAACCCCGGAGACCGTGGAACAAGAAATGGTGGAGGTAGGTGCAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

269

Amino Acids

28.95

Weight (kDa)

5.76

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 51 - 231 6.3e-53 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 582
AccB1I GGYRCC 1 cut(s) 582
AciI CCGC 4 cut(s) 57, 200, 529, 586
AclWI GGATC 1 cut(s) 550
AcoI YGGCCR 2 cut(s) 147, 405
AcsI RAATTY 2 cut(s) 328, 375
AfaI GTAC 2 cut(s) 419, 584
AfiI CCNNNNNNNGG 3 cut(s) 44, 152, 433
AgsI TTSAA 4 cut(s) 26, 181, 520, 605
AjnI CCWGG 2 cut(s) 481, 570
AluBI AGCT 9 cut(s) 71, 215, 302, 400, 461, 611, 623, 662, 715
AluI AGCT 9 cut(s) 71, 215, 302, 400, 461, 611, 623, 662, 715
Alw26I GTCTC 2 cut(s) 243, 764
AlwI GGATC 1 cut(s) 550
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 2 cut(s) 147, 405
ApeKI GCWGC 5 cut(s) 54, 71, 302, 611, 803
ApoI RAATTY 2 cut(s) 328, 375
Asp718I GGTACC 1 cut(s) 582
AspLEI GCGC 3 cut(s) 37, 76, 372
AspS9I GGNCC 4 cut(s) 88, 450, 479, 695
AsuC2I CCSGG 1 cut(s) 767
AsuHPI GGTGA 2 cut(s) 151, 543
AsuNHI GCTAGC 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 210
AvaII GGWCC 4 cut(s) 88, 450, 479, 695
BalI TGGCCA 1 cut(s) 407
BanI GGYRCC 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 4 cut(s) 58, 66, 289, 598
BccI CCATC 4 cut(s) 104, 130, 179, 650
BceAI ACGGC 2 cut(s) 22, 61
BciT130I CCWGG 2 cut(s) 483, 572
BcnI CCSGG 1 cut(s) 767
BcoDI GTCTC 2 cut(s) 243, 764
BfaI CTAG 3 cut(s) 216, 624, 663
BisI GCNGC 6 cut(s) 55, 72, 201, 303, 612, 804
BlsI GCNGC 6 cut(s) 56, 73, 202, 304, 613, 805
Bme1390I CCNGG 3 cut(s) 483, 572, 767
Bme18I GGWCC 4 cut(s) 88, 450, 479, 695
BmeT110I CYCGRG 1 cut(s) 210
BmgT120I GGNCC 4 cut(s) 88, 450, 479, 695
BmiI GGNNCC 4 cut(s) 90, 451, 480, 584
BmrFI CCNGG 3 cut(s) 483, 572, 767
BmsI GCATC 2 cut(s) 401, 516
BmtI GCTAGC 1 cut(s) 219
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 741
BpuMI CCSGG 1 cut(s) 767
BsaBI GATNNNNATC 1 cut(s) 232
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 4 cut(s) 150, 427, 765, 774
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 152, 433
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 678
Bse8I GATNNNNATC 1 cut(s) 232
BseBI CCWGG 2 cut(s) 483, 572
BseDI CCNNGG 4 cut(s) 150, 427, 765, 774
BseGI GGATG 4 cut(s) 190, 392, 475, 531
BseJI GATNNNNATC 1 cut(s) 232
BseLI CCNNNNNNNGG 3 cut(s) 44, 152, 433
BseMII CTCAG 2 cut(s) 254, 606
BseNI ACTGG 1 cut(s) 678
BseXI GCAGC 4 cut(s) 58, 66, 289, 598
BseYI CCCAGC 1 cut(s) 461
BsgI GTGCAG 1 cut(s) 364
BshFI GGCC 2 cut(s) 149, 407
BshNI GGYRCC 1 cut(s) 582
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 2 cut(s) 146, 767
BslFI GGGAC 3 cut(s) 74, 169, 463
BslI CCNNNNNNNGG 3 cut(s) 44, 152, 433
BsmAI GTCTC 2 cut(s) 243, 764
BsmBI CGTCTC 1 cut(s) 243
BsmFI GGGAC 3 cut(s) 74, 169, 463
BsnI GGCC 2 cut(s) 149, 407
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 1 cut(s) 210
Bsp143I GATC 1 cut(s) 542
BspACI CCGC 4 cut(s) 57, 200, 529, 586
BspANI GGCC 2 cut(s) 149, 407
BspCNI CTCAG 2 cut(s) 253, 607
BspLI GGNNCC 4 cut(s) 90, 451, 480, 584
BspOI GCTAGC 1 cut(s) 219
BspPI GGATC 1 cut(s) 550
BspT107I GGYRCC 1 cut(s) 582
BspTNI GGTCTC 1 cut(s) 764
BsrFI RCCGGY 1 cut(s) 145
BsrI ACTGG 1 cut(s) 678
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 4 cut(s) 150, 427, 765, 774
BssMI GATC 1 cut(s) 542
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 2 cut(s) 483, 572
Bst4CI ACNGT 4 cut(s) 311, 428, 503, 775
BstC8I GCNNGC 2 cut(s) 147, 217
BstDEI CTNAG 2 cut(s) 240, 615
BstDSI CCRYGG 2 cut(s) 427, 774
BstF5I GGATG 4 cut(s) 190, 392, 475, 531
BstHHI GCGC 3 cut(s) 37, 76, 372
BstKTI GATC 1 cut(s) 545
BstMAI GTCTC 2 cut(s) 243, 764
BstMBI GATC 1 cut(s) 542
BstMWI GCNNNNNNNGC 4 cut(s) 54, 299, 620, 629
BstNI CCWGG 2 cut(s) 483, 572
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 3 cut(s) 481, 570, 765
BstV1I GCAGC 4 cut(s) 58, 66, 289, 598
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 542
BstXI CCANNNNNNTGG 2 cut(s) 403, 705
BstYI RGATCY 1 cut(s) 542
BsuRI GGCC 2 cut(s) 149, 407
BtgI CCRYGG 2 cut(s) 427, 774
BtsCI GGATG 4 cut(s) 190, 392, 475, 531
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 3 cut(s) 30, 508, 685
Cac8I GCNNGC 2 cut(s) 147, 217
CfoI GCGC 3 cut(s) 37, 76, 372
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 4 cut(s) 88, 450, 479, 695
Csp6I GTAC 2 cut(s) 418, 583
CviAII CATG 5 cut(s) 220, 277, 454, 641, 689
CviQI GTAC 2 cut(s) 418, 583
DdeI CTNAG 2 cut(s) 240, 615
DpnI GATC 1 cut(s) 544
DpnII GATC 1 cut(s) 542
EaeI YGGCCR 2 cut(s) 147, 405
Eco130I CCWWGG 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 764
Eco47I GGWCC 4 cut(s) 88, 450, 479, 695
Eco88I CYCGRG 1 cut(s) 210
EcoO109I RGGNCCY 2 cut(s) 88, 479
EcoRII CCWGG 2 cut(s) 481, 570
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 243
FaeI CATG 5 cut(s) 223, 280, 457, 644, 692
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 3 cut(s) 74, 169, 463
FatI CATG 5 cut(s) 219, 276, 453, 640, 688
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 6 cut(s) 55, 72, 201, 303, 612, 804
FokI GGATG 4 cut(s) 197, 379, 482, 538
Fsp4HI GCNGC 6 cut(s) 55, 72, 201, 303, 612, 804
FspBI CTAG 3 cut(s) 216, 624, 663
GlaI GCGC 3 cut(s) 36, 75, 371
GluI GCNGC 6 cut(s) 55, 72, 201, 303, 612, 804
GsaI CCCAGC 1 cut(s) 465
GsuI CTGGAG 1 cut(s) 741
HaeIII GGCC 2 cut(s) 149, 407
HapII CCGG 2 cut(s) 146, 767
HhaI GCGC 3 cut(s) 37, 76, 372
Hin1II CATG 5 cut(s) 223, 280, 457, 644, 692
Hin6I GCGC 3 cut(s) 35, 74, 370
HinP1I GCGC 3 cut(s) 35, 74, 370
HinfI GANTC 1 cut(s) 206
HpaII CCGG 2 cut(s) 146, 767
HphI GGTGA 2 cut(s) 151, 543
Hpy166II GTNNAC 3 cut(s) 499, 551, 685
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 4 cut(s) 133, 210, 353, 720
Hpy8I GTNNAC 3 cut(s) 499, 551, 685
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 1 cut(s) 507
HpyCH4III ACNGT 4 cut(s) 311, 428, 503, 775
HpyCH4V TGCA 5 cut(s) 170, 345, 392, 644, 803
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 299, 620, 629
HpyF3I CTNAG 2 cut(s) 240, 615
Hsp92II CATG 5 cut(s) 223, 280, 457, 644, 692
HspAI GCGC 3 cut(s) 35, 74, 370
KpnI GGTACC 1 cut(s) 586
KroI GCCGGC 1 cut(s) 145
KroNI GCCGGC 1 cut(s) 147
Kzo9I GATC 1 cut(s) 542
LmnI GCTCC 2 cut(s) 299, 659
Lsp1109I GCAGC 4 cut(s) 58, 66, 289, 598
LweI GCATC 2 cut(s) 401, 516
MaeI CTAG 3 cut(s) 216, 624, 663
MaeIII GTNAC 3 cut(s) 94, 382, 760
MalI GATC 1 cut(s) 544
MboI GATC 1 cut(s) 542
MboII GAAGA 4 cut(s) 14, 262, 265, 617
MflI RGATCY 1 cut(s) 542
MlsI TGGCCA 1 cut(s) 407
MluCI AATT 3 cut(s) 328, 356, 375
MluNI TGGCCA 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 6 cut(s) 79, 118, 218, 499, 503, 787
Mox20I TGGCCA 1 cut(s) 407
MroNI GCCGGC 1 cut(s) 145
MscI TGGCCA 1 cut(s) 407
MseI TTAA 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 407
MspA1I CMGCKG 2 cut(s) 57, 71
MspI CCGG 2 cut(s) 146, 767
MspR9I CCNGG 3 cut(s) 483, 572, 767
MvaI CCWGG 2 cut(s) 483, 572
MwoI GCNNNNNNNGC 4 cut(s) 54, 299, 620, 629
NaeI GCCGGC 1 cut(s) 147
NciI CCSGG 1 cut(s) 767
NdeI CATATG 1 cut(s) 556
NdeII GATC 1 cut(s) 542
NgoMIV GCCGGC 1 cut(s) 145
NheI GCTAGC 1 cut(s) 215
NlaIII CATG 5 cut(s) 223, 280, 457, 644, 692
NlaIV GGNNCC 4 cut(s) 90, 451, 480, 584
NmeAIII GCCGAG 1 cut(s) 24
NspI RCATGY 1 cut(s) 280
PaeR7I CTCGAG 1 cut(s) 210
PdiI GCCGGC 1 cut(s) 147
PfeI GAWTC 1 cut(s) 206
PfoI TCCNGGA 2 cut(s) 481, 570
PkrI GCNGC 6 cut(s) 56, 73, 202, 304, 613, 805
PpuMI RGGWCCY 2 cut(s) 88, 479
Psp5II RGGWCCY 2 cut(s) 88, 479
Psp6I CCWGG 2 cut(s) 481, 570
PspFI CCCAGC 1 cut(s) 461
PspGI CCWGG 2 cut(s) 481, 570
PspN4I GGNNCC 4 cut(s) 90, 451, 480, 584
PspPI GGNCC 4 cut(s) 88, 450, 479, 695
PspPPI RGGWCCY 2 cut(s) 88, 479
PsuI RGATCY 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 2 cut(s) 419, 584
RsaNI GTAC 2 cut(s) 418, 583
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 6 cut(s) 55, 72, 201, 303, 612, 804
Sau3AI GATC 1 cut(s) 542
Sau96I GGNCC 4 cut(s) 88, 450, 479, 695
ScrFI CCNGG 3 cut(s) 483, 572, 767
SfaNI GCATC 2 cut(s) 401, 516
Sfr274I CTCGAG 1 cut(s) 210
SinI GGWCC 4 cut(s) 88, 450, 479, 695
SlaI CTCGAG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
Sse9I AATT 3 cut(s) 328, 356, 375
SsiI CCGC 4 cut(s) 57, 200, 529, 586
SspMI CTAG 3 cut(s) 216, 624, 663
StyD4I CCNGG 3 cut(s) 481, 570, 765
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 4 cut(s) 311, 428, 503, 775
TaqI TCGA 4 cut(s) 9, 84, 211, 261
TaqII GACCGA 1 cut(s) 683
TasI AATT 3 cut(s) 328, 356, 375
TatI WGTACW 1 cut(s) 417
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 3 cut(s) 37, 508, 685
TseI GCWGC 5 cut(s) 54, 71, 302, 611, 803
TspDTI ATGAA 2 cut(s) 17, 486
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 3 cut(s) 37, 508, 685
VpaK11BI GGWCC 4 cut(s) 88, 450, 479, 695
XapI RAATTY 2 cut(s) 328, 375
XceI RCATGY 1 cut(s) 280
XhoI CTCGAG 1 cut(s) 210
XspI CTAG 3 cut(s) 216, 624, 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.