Rroxscaffold_1G00072510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000613
Physical Location & Seq
Reverse (-)
93555638 .. 93558265
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00072510.1

Sequence Viewer

Length: 732 bp
ATGGGTTGCATGGTTTCAAAACACTCTTCGAACTTGTCCCTCAAGGGGACAACCGCTCTTGCTTTTTTCCACAAAGAGGGCATTGTAGTTGCAGTTGACTCCAGGGCTTCCAACGAAGAGGATGAGCTTGAAAAAGAGCTTGATAGTAATCTTTCTCGAGATGAATATGCTGGCAATGAACCTAGAGTTTCAGATGTAGCAGAAACAATTTATAAGTATTTAAATGGTCTTCGTGGCGAGAATAAAAGGTTTGGAATCCTCGTCGCGGGATGGGATGCAGAGGGTCACGATCAAATTTATCGTCTGTTTATAAGAGATGATGTTGTTGTGAAACAAAAAGTAACTTCCTCACGATTTGGAGTACTAGGATCTGGGCATGTCTATGCTGCTACTAGTCTTAAAAATGAGAAACGAATCTCGGGACCGGCCCGATTGGAGGAGCAGCGGTTATCCTTCCACCGTCTTATATTCAACTCTGGAGTTGAGGCTTCAAATGCATATGCACTCACAGCTATTCATGGTGAAACTGATTCTCAACTTGTAGAAAAGTTTCCAAATCAATTATTGTTGAAAACTGCGTTTCAAAAAAAATTGTTGTTGAAAACTGTGGAGCCAAGTGTTGAACTCTATTTGAATCGGAGCTCAAAAGATTTGCTCAAGTTCATACGCACCCAATTTGATGGATATTTGTCAACAGAGATGACACAAATGACCGGAGCACTCGGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

27.14

Weight (kDa)

6.61

Isoelectric Point (pI)

30.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 213, 311
AccBSI CCGCTC 1 cut(s) 56
AccII CGCG 1 cut(s) 266
AciI CCGC 3 cut(s) 54, 266, 445
AclWI GGATC 1 cut(s) 376
AcsI RAATTY 1 cut(s) 294
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 4 cut(s) 45, 76, 265, 436
AgsI TTSAA 9 cut(s) 18, 131, 472, 492, 571, 584, 601, 623, 634
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 4 cut(s) 127, 139, 512, 642
AluI AGCT 4 cut(s) 127, 139, 512, 642
Alw21I GWGCWC 2 cut(s) 644, 721
AlwI GGATC 1 cut(s) 376
Ama87I CYCGRG 2 cut(s) 156, 418
AoxI GGCC 2 cut(s) 426, 724
ApeKI GCWGC 2 cut(s) 386, 442
ApoI RAATTY 1 cut(s) 294
ArsI GACNNNNNNTTYG 2 cut(s) 286, 318
Asp700I GAANNNNTTC 1 cut(s) 549
AspS9I GGNCC 2 cut(s) 422, 427
AsuHPI GGTGA 1 cut(s) 533
AsuII TTCGAA 1 cut(s) 29
AvaI CYCGRG 2 cut(s) 156, 418
AvaII GGWCC 1 cut(s) 422
BanII GRGCYC 1 cut(s) 644
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 2 cut(s) 644, 721
BbvI GCAGC 2 cut(s) 373, 454
BccI CCATC 2 cut(s) 264, 674
BciT130I CCWGG 1 cut(s) 103
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 3 cut(s) 183, 365, 393
BisI GCNGC 2 cut(s) 387, 443
BlsI GCNGC 2 cut(s) 388, 444
BmcAI AGTACT 1 cut(s) 363
Bme1390I CCNGG 1 cut(s) 103
Bme18I GGWCC 1 cut(s) 422
BmeT110I CYCGRG 2 cut(s) 156, 418
BmgT120I GGNCC 2 cut(s) 422, 427
BmiI GGNNCC 2 cut(s) 423, 612
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 1 cut(s) 265
BpiI GAAGAC 1 cut(s) 221
BpmI CTGGAG 2 cut(s) 85, 498
Bpu14I TTCGAA 1 cut(s) 29
BpuEI CTTGAG 2 cut(s) 26, 641
BsaBI GATNNNNATC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 102
BsaWI WCCGGW 1 cut(s) 713
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 76, 265, 436
Bse118I RCCGGY 1 cut(s) 424
Bse3DI GCAATG 1 cut(s) 181
Bse8I GATNNNNATC 1 cut(s) 147
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 3 cut(s) 127, 275, 280
BseJI GATNNNNATC 1 cut(s) 147
BseLI CCNNNNNNNGG 4 cut(s) 45, 76, 265, 436
BseMI GCAATG 1 cut(s) 181
BseRI GAGGAG 1 cut(s) 452
BseXI GCAGC 2 cut(s) 373, 454
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 2 cut(s) 428, 726
BsiHKAI GWGCWC 2 cut(s) 644, 721
BsiHKCI CYCGRG 2 cut(s) 156, 418
BsiSI CCGG 2 cut(s) 425, 714
BslFI GGGAC 3 cut(s) 22, 61, 435
BslI CCNNNNNNNGG 4 cut(s) 45, 76, 265, 436
BsmFI GGGAC 3 cut(s) 22, 61, 435
BsnI GGCC 2 cut(s) 428, 726
BsoBI CYCGRG 2 cut(s) 156, 418
Bsp119I TTCGAA 1 cut(s) 29
Bsp1286I GDGCHC 2 cut(s) 644, 721
Bsp143I GATC 2 cut(s) 289, 368
BspACI CCGC 3 cut(s) 54, 266, 445
BspANI GGCC 2 cut(s) 428, 726
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 2 cut(s) 423, 612
BspPI GGATC 1 cut(s) 376
BspT104I TTCGAA 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 181
BsrFI RCCGGY 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 2 cut(s) 289, 368
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 2 cut(s) 461, 607
Bst6I CTCTTC 2 cut(s) 31, 111
BstBI TTCGAA 1 cut(s) 29
BstC8I GCNNGC 1 cut(s) 172
BstF5I GGATG 3 cut(s) 127, 275, 280
BstFNI CGCG 1 cut(s) 266
BstKTI GATC 2 cut(s) 292, 371
BstMBI GATC 2 cut(s) 289, 368
BstMWI GCNNNNNNNGC 2 cut(s) 494, 509
BstNI CCWGG 1 cut(s) 103
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 101
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 2 cut(s) 373, 454
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 368
BstXI CCANNNNNNTGG 1 cut(s) 680
BstYI RGATCY 1 cut(s) 368
BsuRI GGCC 2 cut(s) 428, 726
BtsCI GGATG 3 cut(s) 127, 275, 280
Cac8I GCNNGC 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 422, 427
Csp6I GTAC 1 cut(s) 362
CviAII CATG 3 cut(s) 10, 377, 518
CviJI RGCY 9 cut(s) 107, 127, 139, 428, 488, 512, 613, 642, 726
CviKI_1 RGCY 9 cut(s) 107, 127, 139, 428, 488, 512, 613, 642, 726
CviQI GTAC 1 cut(s) 362
DpnI GATC 2 cut(s) 291, 370
DpnII GATC 2 cut(s) 289, 368
DraI TTTAAA 1 cut(s) 222
Eam1104I CTCTTC 2 cut(s) 31, 111
EarI CTCTTC 2 cut(s) 31, 111
Ecl136II GAGCTC 1 cut(s) 642
Eco24I GRGCYC 1 cut(s) 644
Eco47I GGWCC 1 cut(s) 422
Eco53kI GAGCTC 1 cut(s) 642
Eco88I CYCGRG 2 cut(s) 156, 418
EcoICRI GAGCTC 1 cut(s) 642
EcoRII CCWGG 1 cut(s) 101
EcoT22I ATGCAT 1 cut(s) 499
EcoT38I GRGCYC 1 cut(s) 644
FaeI CATG 3 cut(s) 13, 380, 521
FaqI GGGAC 3 cut(s) 22, 61, 435
FatI CATG 3 cut(s) 9, 376, 517
FauI CCCGC 1 cut(s) 259
FauNDI CATATG 1 cut(s) 499
Fnu4HI GCNGC 2 cut(s) 387, 443
FokI GGATG 3 cut(s) 134, 282, 287
FriOI GRGCYC 1 cut(s) 644
Fsp4HI GCNGC 2 cut(s) 387, 443
FspBI CTAG 3 cut(s) 183, 365, 393
GluI GCNGC 2 cut(s) 387, 443
GsuI CTGGAG 2 cut(s) 85, 498
HaeIII GGCC 2 cut(s) 428, 726
HapII CCGG 2 cut(s) 425, 714
Hin1II CATG 3 cut(s) 13, 380, 521
HincII GTYRAC 2 cut(s) 97, 693
HindII GTYRAC 2 cut(s) 97, 693
HinfI GANTC 5 cut(s) 98, 255, 414, 530, 634
HpaII CCGG 2 cut(s) 425, 714
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 2 cut(s) 97, 693
Hpy188I TCNGA 2 cut(s) 193, 639
Hpy188III TCNNGA 6 cut(s) 156, 158, 287, 351, 420, 477
Hpy8I GTNNAC 2 cut(s) 97, 693
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 2 cut(s) 461, 607
HpyCH4V TGCA 5 cut(s) 9, 92, 278, 497, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 509
Hsp92II CATG 3 cut(s) 13, 380, 521
Kzo9I GATC 2 cut(s) 289, 368
LmnI GCTCC 4 cut(s) 439, 610, 639, 716
LpnPI CCDG 7 cut(s) 88, 115, 156, 357, 438, 462, 727
Lsp1109I GCAGC 2 cut(s) 373, 454
LweI GCATC 1 cut(s) 265
MaeI CTAG 3 cut(s) 183, 365, 393
MaeIII GTNAC 2 cut(s) 284, 340
MalI GATC 2 cut(s) 291, 370
MbiI CCGCTC 1 cut(s) 56
MboI GATC 2 cut(s) 289, 368
MboII GAAGA 3 cut(s) 18, 128, 221
MflI RGATCY 1 cut(s) 368
MhlI GDGCHC 2 cut(s) 644, 721
MluCI AATT 5 cut(s) 207, 294, 560, 590, 674
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 8 cut(s) 50, 70, 112, 269, 274, 358, 430, 478
Mph1103I ATGCAT 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 549
MseI TTAA 2 cut(s) 221, 399
MslI CAYNNNNRTG 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 445
MspI CCGG 2 cut(s) 425, 714
MspR9I CCNGG 1 cut(s) 103
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 494, 509
NdeI CATATG 1 cut(s) 499
NdeII GATC 2 cut(s) 289, 368
NlaIII CATG 3 cut(s) 13, 380, 521
NlaIV GGNNCC 2 cut(s) 423, 612
NmeAIII GCCGAG 1 cut(s) 702
NmuCI GTSAC 1 cut(s) 284
NsiI ATGCAT 1 cut(s) 499
NspI RCATGY 1 cut(s) 380
NspV TTCGAA 1 cut(s) 29
PaeR7I CTCGAG 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 4 cut(s) 255, 414, 530, 634
PkrI GCNGC 2 cut(s) 388, 444
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PsiI TTATAA 2 cut(s) 213, 311
Psp124BI GAGCTC 1 cut(s) 644
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 423, 612
PspPI GGNCC 2 cut(s) 422, 427
PsuI RGATCY 1 cut(s) 368
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
RseI CAYNNNNRTG 1 cut(s) 381
SacI GAGCTC 1 cut(s) 644
SaqAI TTAA 2 cut(s) 221, 399
SatI GCNGC 2 cut(s) 387, 443
Sau3AI GATC 2 cut(s) 289, 368
Sau96I GGNCC 2 cut(s) 422, 427
ScaI AGTACT 1 cut(s) 363
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 103
SduI GDGCHC 2 cut(s) 644, 721
SetI ASST 6 cut(s) 129, 141, 184, 251, 514, 644
SfaNI GCATC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 156
SfuI TTCGAA 1 cut(s) 29
SinI GGWCC 1 cut(s) 422
SlaI CTCGAG 1 cut(s) 156
SmiI ATTTAAAT 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 3 cut(s) 41, 156, 656
SmoI CTYRAG 3 cut(s) 41, 156, 656
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 5 cut(s) 207, 294, 560, 590, 674
SsiI CCGC 3 cut(s) 54, 266, 445
SspMI CTAG 3 cut(s) 183, 365, 393
SstI GAGCTC 1 cut(s) 644
StyD4I CCNGG 1 cut(s) 101
SwaI ATTTAAAT 1 cut(s) 222
TaaI ACNGT 2 cut(s) 461, 607
TaqI TCGA 2 cut(s) 29, 157
TasI AATT 5 cut(s) 207, 294, 560, 590, 674
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 4 cut(s) 255, 414, 530, 634
Tru1I TTAA 2 cut(s) 221, 399
Tru9I TTAA 2 cut(s) 221, 399
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 2 cut(s) 386, 442
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 4 cut(s) 177, 192, 506, 652
VpaK11BI GGWCC 1 cut(s) 422
XapI RAATTY 1 cut(s) 294
XceI RCATGY 1 cut(s) 380
XhoI CTCGAG 1 cut(s) 156
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 3 cut(s) 183, 365, 393
ZrmI AGTACT 1 cut(s) 363
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.