RLG00000031116

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000613
Physical Location & Seq
Reverse (-)
2261195 .. 2263571
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031116

Sequence Viewer

Length: 1212 bp
ATGGATGGGAGCACTGGTTTTCGGTTCATCGTTAGGCACGCGTGGGCGGGGCTATTTCCAAGCGGCGGTGGCAGTGCAGCAGCGTCTGCTCGTTTGGCTGCGTCAAGATCCAAGATGGGTTGTGGGGCTTCAAAATATTCTTCGGAAGGATCAGAAAGCAGGAAATTTGAGAATACGTCACTAGCTTTGGACCATAAACTCAAAGGGACGACACTTATTGGTTTTGTTTACCGTGAGGGCATCATAGTTGGAGCTGAATCTAGGGCTTCCAATGAATATGATGAGCTTCTATATCCGCTTACAATTCGAGACAACACACCCAAAATGGCTGAAATATCTTCCCACATAATTTACGTAGTTCAAGGGGTAGAGGCTGATTGTACACAAATGAGCTTGCATATAAGTGAGAAGTTTCCGGACATGGATGAACATCTTGGCAATGCACCTACAGTTTCATGTGTAGTAGAAACGGTGTATAAGTATCTGAACAGTCTCGATGACAAGAATAAAGTATGTGGAGCCATCATCGCTGGATGGGATGCTTCGGGTCCTCAAGTTCATCGTGTTTTTCTAAAAAACAAAAAGGTTGTGGAAGAAAGAGCTTGTATGCCTGCAGCGGTAGGAAGTGGTCAAGTTCATGCTCTTCGTATCCTTAATAAAAGTTACAACTACAATATGTCCACTGCTGATGCTATTGATTTGGCGCGTAAAGCTTTATTTAATGCAACCTACTATGACAAGCATAGTGGTGGAGAACTGAAAGTTTATCACATTAATAATTCTGAATGGGAGCCGCTACCTATCATGAATGCTTTGGAAGCTTACACAAGATATTACGACCTTCACTGTCGCTATGAACGGAAAACGCTTTTCTTGGTTGTGGATGCCGGGATCCAACCTATTTCTGCAAATGACCTTATAGAACACTTCAAGCCACATGCAAAGTTATTCGCATCCCATCGTGTAGCCCTCTGTAAATTGGGAGGTGATCGCCTCTACTTCCACCGCCTTGTGTTCGAGTCTGACGATGATGCAGAAAGGGCATATGAAACTACAACTCCTCATGGTCGAACTACTCCCTCGTACCTAGAACGGTTTCCAGATCAAGTGGTCTTGGATAATAAGAATCCAAGTGTTACAGTTTATGTGAATTGGAGCTCAAGGGATTTGCTGGAATTTGTACACGATGAATGTCAGTTATTATATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

404

Amino Acids

45.06

Weight (kDa)

6.25

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 68 - 258 4.4e-22 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 41, 706
AccIII TCCGGA 1 cut(s) 415
AciI CCGC 7 cut(s) 47, 63, 66, 296, 617, 794, 1006
AclWI GGATC 4 cut(s) 102, 157, 886, 899
AcsI RAATTY 2 cut(s) 164, 1175
AfaI GTAC 3 cut(s) 382, 1085, 1182
AfiI CCNNNNNNNGG 1 cut(s) 65
AflIII ACRYGT 1 cut(s) 39
AgsI TTSAA 3 cut(s) 132, 362, 931
AluBI AGCT 8 cut(s) 185, 254, 286, 393, 602, 713, 821, 1158
AluI AGCT 8 cut(s) 185, 254, 286, 393, 602, 713, 821, 1158
Alw21I GWGCWC 2 cut(s) 14, 1160
Alw26I GTCTC 2 cut(s) 303, 497
AlwI GGATC 4 cut(s) 102, 157, 886, 899
AlwNI CAGNNNCTG 1 cut(s) 86
Aor13HI TCCGGA 1 cut(s) 415
ApeKI GCWGC 4 cut(s) 77, 80, 98, 614
ApoI RAATTY 2 cut(s) 164, 1175
AseI ATTAAT 1 cut(s) 774
Asp700I GAANNNNTTC 1 cut(s) 1095
AspLEI GCGC 1 cut(s) 706
AspS9I GGNCC 2 cut(s) 190, 548
AsuC2I CCSGG 1 cut(s) 889
AsuHPI GGTGA 1 cut(s) 998
AvaII GGWCC 2 cut(s) 190, 548
BamHI GGATCC 1 cut(s) 891
BanII GRGCYC 1 cut(s) 1160
Bbv12I GWGCWC 2 cut(s) 14, 1160
BbvI GCAGC 4 cut(s) 85, 89, 92, 626
BccI CCATC 4 cut(s) 109, 528, 530, 966
BciVI GTATCC 1 cut(s) 659
BcnI CCSGG 1 cut(s) 889
BcoDI GTCTC 2 cut(s) 303, 497
BfaI CTAG 3 cut(s) 182, 261, 1088
BfmI CTRYAG 2 cut(s) 447, 612
BfuI GTATCC 1 cut(s) 659
BisI GCNGC 6 cut(s) 64, 78, 81, 99, 615, 794
BlsI GCNGC 6 cut(s) 65, 79, 82, 100, 616, 795
Bme1390I CCNGG 1 cut(s) 889
Bme18I GGWCC 2 cut(s) 190, 548
BmgT120I GGNCC 2 cut(s) 190, 548
BmiI GGNNCC 4 cut(s) 520, 549, 792, 893
BmrFI CCNGG 1 cut(s) 889
BmsI GCATC 6 cut(s) 249, 529, 679, 874, 962, 1021
BpuEI CTTGAG 2 cut(s) 537, 1144
BpuMI CCSGG 1 cut(s) 889
BsaAI YACGTR 1 cut(s) 355
BsaBI GATNNNNATC 1 cut(s) 429
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 3 cut(s) 31, 1042, 1072
Bsc4I CCNNNNNNNGG 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 19
Bse3DI GCAATG 1 cut(s) 445
Bse8I GATNNNNATC 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 415
BseGI GGATG 6 cut(s) 10, 430, 539, 544, 889, 953
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 1 cut(s) 65
BseMI GCAATG 1 cut(s) 445
BseNI ACTGG 1 cut(s) 19
BseRI GAGGAG 1 cut(s) 1050
BseXI GCAGC 4 cut(s) 85, 89, 92, 626
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 2 cut(s) 41, 706
BsiHKAI GWGCWC 2 cut(s) 14, 1160
BsiSI CCGG 2 cut(s) 416, 888
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 65
BsmAI GTCTC 2 cut(s) 303, 497
BsmFI GGGAC 1 cut(s) 220
BsmI GAATGC 1 cut(s) 814
Bsp1286I GDGCHC 2 cut(s) 14, 1160
Bsp13I TCCGGA 1 cut(s) 415
Bsp1407I TGTACA 2 cut(s) 380, 1180
Bsp143I GATC 5 cut(s) 107, 149, 891, 988, 1102
BspACI CCGC 7 cut(s) 47, 63, 66, 296, 617, 794, 1006
BspEI TCCGGA 1 cut(s) 415
BspFNI CGCG 2 cut(s) 41, 706
BspHI TCATGA 1 cut(s) 804
BspLI GGNNCC 4 cut(s) 520, 549, 792, 893
BspMAI CTGCAG 1 cut(s) 616
BspPI GGATC 4 cut(s) 102, 157, 886, 899
BspQI GCTCTTC 1 cut(s) 648
BsrDI GCAATG 1 cut(s) 445
BsrGI TGTACA 2 cut(s) 380, 1180
BsrI ACTGG 1 cut(s) 19
BssMI GATC 5 cut(s) 107, 149, 891, 988, 1102
Bst4CI ACNGT 7 cut(s) 233, 451, 472, 491, 848, 1095, 1141
Bst6I CTCTTC 1 cut(s) 648
BstAPI GCANNNNNTGC 1 cut(s) 86
BstAUI TGTACA 2 cut(s) 380, 1180
BstBAI YACGTR 1 cut(s) 355
BstC8I GCNNGC 3 cut(s) 39, 395, 612
BstF5I GGATG 6 cut(s) 10, 430, 539, 544, 889, 953
BstFNI CGCG 2 cut(s) 41, 706
BstHHI GCGC 1 cut(s) 706
BstKTI GATC 5 cut(s) 110, 152, 894, 991, 1105
BstMAI GTCTC 2 cut(s) 303, 497
BstMBI GATC 5 cut(s) 107, 149, 891, 988, 1102
BstMWI GCNNNNNNNGC 7 cut(s) 69, 86, 95, 527, 710, 818, 1040
BstNSI RCATGY 1 cut(s) 941
BstSCI CCNGG 1 cut(s) 887
BstSFI CTRYAG 2 cut(s) 447, 612
BstSNI TACGTA 1 cut(s) 355
BstUI CGCG 2 cut(s) 41, 706
BstV1I GCAGC 4 cut(s) 85, 89, 92, 626
BstX2I RGATCY 2 cut(s) 107, 891
BstYI RGATCY 2 cut(s) 107, 891
BsuI GTATCC 1 cut(s) 659
BtgZI GCGATG 1 cut(s) 511
BtsCI GGATG 6 cut(s) 10, 430, 539, 544, 889, 953
BtsI GCAGTG 2 cut(s) 79, 681
BtsIMutI CAGTG 4 cut(s) 12, 79, 681, 844
Cac8I GCNNGC 3 cut(s) 39, 395, 612
CaiI CAGNNNCTG 1 cut(s) 86
CciI TCATGA 1 cut(s) 804
CfoI GCGC 1 cut(s) 706
Cfr13I GGNCC 2 cut(s) 190, 548
CseI GACGC 2 cut(s) 72, 90
Csp6I GTAC 3 cut(s) 381, 1084, 1181
CspCI CAANNNNNGTGG 2 cut(s) 727, 762
CviAII CATG 6 cut(s) 421, 456, 638, 805, 938, 1064
CviQI GTAC 3 cut(s) 381, 1084, 1181
DpnI GATC 5 cut(s) 109, 151, 893, 990, 1104
DpnII GATC 5 cut(s) 107, 149, 891, 988, 1102
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
Ecl136II GAGCTC 1 cut(s) 1158
Eco105I TACGTA 1 cut(s) 355
Eco24I GRGCYC 1 cut(s) 1160
Eco47I GGWCC 2 cut(s) 190, 548
Eco53kI GAGCTC 1 cut(s) 1158
EcoICRI GAGCTC 1 cut(s) 1158
EcoO109I RGGNCCY 1 cut(s) 548
EcoT38I GRGCYC 1 cut(s) 1160
FaeI CATG 6 cut(s) 424, 459, 641, 808, 941, 1067
FaqI GGGAC 1 cut(s) 220
FatI CATG 6 cut(s) 420, 455, 637, 804, 937, 1063
FauI CCCGC 1 cut(s) 40
FauNDI CATATG 1 cut(s) 1045
Fnu4HI GCNGC 6 cut(s) 64, 78, 81, 99, 615, 794
FokI GGATG 6 cut(s) 17, 437, 546, 551, 896, 940
FriOI GRGCYC 1 cut(s) 1160
Fsp4HI GCNGC 6 cut(s) 64, 78, 81, 99, 615, 794
FspBI CTAG 3 cut(s) 182, 261, 1088
GlaI GCGC 1 cut(s) 705
GluI GCNGC 6 cut(s) 64, 78, 81, 99, 615, 794
HapII CCGG 2 cut(s) 416, 888
HgaI GACGC 2 cut(s) 72, 90
HhaI GCGC 1 cut(s) 706
Hin1II CATG 6 cut(s) 424, 459, 641, 808, 941, 1067
Hin6I GCGC 1 cut(s) 704
HinP1I GCGC 1 cut(s) 704
HindIII AAGCTT 2 cut(s) 711, 819
HinfI GANTC 3 cut(s) 257, 1019, 1126
HpaII CCGG 2 cut(s) 416, 888
HphI GGTGA 1 cut(s) 998
Hpy166II GTNNAC 4 cut(s) 229, 383, 681, 1183
Hpy188I TCNGA 5 cut(s) 145, 154, 486, 784, 1024
Hpy188III TCNNGA 6 cut(s) 105, 308, 416, 494, 805, 1100
Hpy8I GTNNAC 4 cut(s) 229, 383, 681, 1183
HpyAV CCTTC 2 cut(s) 140, 851
HpyCH4III ACNGT 7 cut(s) 233, 451, 472, 491, 848, 1095, 1141
HpyCH4IV ACGT 2 cut(s) 176, 354
HpyCH4V TGCA 8 cut(s) 77, 397, 443, 614, 725, 908, 941, 1034
HpyF10VI GCNNNNNNNGC 7 cut(s) 69, 86, 95, 527, 710, 818, 1040
HpySE526I ACGT 2 cut(s) 176, 354
Hsp92II CATG 6 cut(s) 424, 459, 641, 808, 941, 1067
HspAI GCGC 1 cut(s) 704
Kpn2I TCCGGA 1 cut(s) 415
Kzo9I GATC 5 cut(s) 107, 149, 891, 988, 1102
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 5 cut(s) 9, 251, 518, 790, 1155
LpnPI CCDG 7 cut(s) 145, 429, 516, 624, 901, 1113, 1157
Lsp1109I GCAGC 4 cut(s) 85, 89, 92, 626
LweI GCATC 6 cut(s) 249, 529, 679, 874, 962, 1021
MaeI CTAG 3 cut(s) 182, 261, 1088
MaeII ACGT 2 cut(s) 176, 354
MaeIII GTNAC 3 cut(s) 177, 662, 1135
MalI GATC 5 cut(s) 109, 151, 893, 990, 1104
MboI GATC 5 cut(s) 107, 149, 891, 988, 1102
MboII GAAGA 4 cut(s) 132, 330, 605, 635
MflI RGATCY 2 cut(s) 107, 891
MhlI GDGCHC 2 cut(s) 14, 1160
MluCI AATT 7 cut(s) 164, 303, 348, 778, 977, 1150, 1175
MluI ACGCGT 1 cut(s) 39
MlyI GAGTC 1 cut(s) 1028
MmeI TCCRAC 2 cut(s) 229, 919
MnlI CCTC 8 cut(s) 229, 364, 561, 977, 980, 1004, 1071, 1090
MroI TCCGGA 1 cut(s) 415
MroXI GAANNNNTTC 1 cut(s) 1095
MseI TTAA 3 cut(s) 654, 720, 774
MslI CAYNNNNRTG 2 cut(s) 402, 747
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 2 cut(s) 416, 888
MspR9I CCNGG 1 cut(s) 889
Mva1269I GAATGC 1 cut(s) 814
MvnI CGCG 2 cut(s) 41, 706
MwoI GCNNNNNNNGC 7 cut(s) 69, 86, 95, 527, 710, 818, 1040
NciI CCSGG 1 cut(s) 889
NdeI CATATG 1 cut(s) 1045
NdeII GATC 5 cut(s) 107, 149, 891, 988, 1102
NlaIII CATG 6 cut(s) 424, 459, 641, 808, 941, 1067
NlaIV GGNNCC 4 cut(s) 520, 549, 792, 893
NmuCI GTSAC 1 cut(s) 177
NspI RCATGY 1 cut(s) 941
PagI TCATGA 1 cut(s) 804
PciSI GCTCTTC 1 cut(s) 648
PcsI WCGNNNNNNNCGW 1 cut(s) 1023
PctI GAATGC 1 cut(s) 814
PdmI GAANNNNTTC 1 cut(s) 1095
PfeI GAWTC 2 cut(s) 257, 1126
PkrI GCNGC 6 cut(s) 65, 79, 82, 100, 616, 795
PleI GAGTC 1 cut(s) 1027
PpsI GAGTC 1 cut(s) 1027
Ppu21I YACGTR 1 cut(s) 355
PpuMI RGGWCCY 1 cut(s) 548
PshBI ATTAAT 1 cut(s) 774
Psp124BI GAGCTC 1 cut(s) 1160
Psp5II RGGWCCY 1 cut(s) 548
PspN4I GGNNCC 4 cut(s) 520, 549, 792, 893
PspPI GGNCC 2 cut(s) 190, 548
PspPPI RGGWCCY 1 cut(s) 548
PstI CTGCAG 1 cut(s) 616
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 2 cut(s) 107, 891
RsaI GTAC 3 cut(s) 382, 1085, 1182
RsaNI GTAC 3 cut(s) 381, 1084, 1181
RseI CAYNNNNRTG 2 cut(s) 402, 747
SacI GAGCTC 1 cut(s) 1160
SapI GCTCTTC 1 cut(s) 648
SaqAI TTAA 3 cut(s) 654, 720, 774
SatI GCNGC 6 cut(s) 64, 78, 81, 99, 615, 794
Sau3AI GATC 5 cut(s) 107, 149, 891, 988, 1102
Sau96I GGNCC 2 cut(s) 190, 548
SchI GAGTC 1 cut(s) 1028
ScrFI CCNGG 1 cut(s) 889
SduI GDGCHC 2 cut(s) 14, 1160
SfaNI GCATC 6 cut(s) 249, 529, 679, 874, 962, 1021
SfcI CTRYAG 2 cut(s) 447, 612
SinI GGWCC 2 cut(s) 190, 548
SmiMI CAYNNNNRTG 2 cut(s) 402, 747
SmlI CTYRAG 2 cut(s) 552, 1159
SmoI CTYRAG 2 cut(s) 552, 1159
SnaBI TACGTA 1 cut(s) 355
Sse9I AATT 7 cut(s) 164, 303, 348, 778, 977, 1150, 1175
SsiI CCGC 7 cut(s) 47, 63, 66, 296, 617, 794, 1006
SspI AATATT 1 cut(s) 137
SspMI CTAG 3 cut(s) 182, 261, 1088
SstI GAGCTC 1 cut(s) 1160
StyD4I CCNGG 1 cut(s) 887
TaaI ACNGT 7 cut(s) 233, 451, 472, 491, 848, 1095, 1141
TaiI ACGT 2 cut(s) 179, 357
TaqI TCGA 4 cut(s) 307, 495, 1017, 1069
TasI AATT 7 cut(s) 164, 303, 348, 778, 977, 1150, 1175
TatI WGTACW 2 cut(s) 380, 1180
TauI GCSGC 2 cut(s) 66, 796
TfiI GAWTC 2 cut(s) 257, 1126
Tru1I TTAA 3 cut(s) 654, 720, 774
Tru9I TTAA 3 cut(s) 654, 720, 774
TscAI CASTG 4 cut(s) 19, 79, 688, 851
TseFI GTSAC 1 cut(s) 177
TseI GCWGC 4 cut(s) 77, 80, 98, 614
Tsp45I GTSAC 1 cut(s) 177
TspGWI ACGGA 1 cut(s) 874
TspRI CASTG 4 cut(s) 19, 79, 688, 851
VpaK11BI GGWCC 2 cut(s) 190, 548
VspI ATTAAT 1 cut(s) 774
XapI RAATTY 2 cut(s) 164, 1175
XceI RCATGY 1 cut(s) 941
XmnI GAANNNNTTC 1 cut(s) 1095
XspI CTAG 3 cut(s) 182, 261, 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.