Rh4AG227500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000613
Physical Location & Seq
Reverse (-)
52854703 .. 52860230
5528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG227500.1

Sequence Viewer

Length: 354 bp
ATGGATATTCACATGTCAATGTGCCGGGTCGAAGATGAGACTGCTCCACCTGATGAAGGTAGCAAAGGCATTGGTTTTGTCATGGAAGATGAAAAAATAATTGTAGCTGGGTTAGGCAGTAAATGGGCATTGATCAAATATATGAGCATACAATGTCGTACTCTACGTACTTCCCATTCTCTATCTGGCTCCAGGAGAGTCCCCGCTGCAGTGCCCTGGAAGATCGCTGCTCTCACGGTCGCTGCGGACGGGAAGATGAACGCAACTCGATCAGTCCCAGCTTCTGGTTTCTTGGTGCCACCGCCTGCCTCTATGCTACTGACAATCGTATATGTGGGCTGTGCAATTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

12.45

Weight (kDa)

8.54

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 295
AccB7I CCANNNNNTGG 1 cut(s) 284
AciI CCGC 3 cut(s) 204, 245, 302
AfaI GTAC 2 cut(s) 160, 169
AfiI CCNNNNNNNGG 2 cut(s) 56, 284
AflIII ACRYGT 1 cut(s) 12
AjnI CCWGG 2 cut(s) 191, 215
AluBI AGCT 2 cut(s) 107, 281
AluI AGCT 2 cut(s) 107, 281
Alw26I GTCTC 1 cut(s) 32
AlwNI CAGNNNCTG 1 cut(s) 284
ApeKI GCWGC 3 cut(s) 206, 227, 242
AsuC2I CCSGG 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 216
BanI GGYRCC 1 cut(s) 295
BbvI GCAGC 3 cut(s) 193, 214, 229
BciT130I CCWGG 2 cut(s) 193, 217
BclI TGATCA 1 cut(s) 132
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 207
BisI GCNGC 3 cut(s) 207, 228, 243
BlsI GCNGC 3 cut(s) 208, 229, 244
Bme1390I CCNGG 3 cut(s) 26, 193, 217
BmiI GGNNCC 2 cut(s) 190, 297
BmrFI CCNGG 3 cut(s) 26, 193, 217
BpmI CTGGAG 1 cut(s) 175
BpuMI CCSGG 1 cut(s) 26
BsaAI YACGTR 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 215
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 284
BseBI CCWGG 2 cut(s) 193, 217
BseDI CCNNGG 1 cut(s) 215
BseLI CCNNNNNNNGG 2 cut(s) 56, 284
BseSI GKGCMC 1 cut(s) 216
BseXI GCAGC 3 cut(s) 193, 214, 229
BseYI CCCAGC 2 cut(s) 107, 277
Bsh1285I CGRYCG 1 cut(s) 240
BshNI GGYRCC 1 cut(s) 295
BsiEI CGRYCG 1 cut(s) 240
BsiSI CCGG 1 cut(s) 25
BslFI GGGAC 2 cut(s) 185, 260
BslI CCNNNNNNNGG 2 cut(s) 56, 284
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 185, 260
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 3 cut(s) 132, 222, 269
BspACI CCGC 3 cut(s) 204, 245, 302
BspLI GGNNCC 2 cut(s) 190, 297
BspMAI CTGCAG 1 cut(s) 211
BspT107I GGYRCC 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 215
BssMI GATC 3 cut(s) 132, 222, 269
Bst2UI CCWGG 2 cut(s) 193, 217
Bst4CI ACNGT 1 cut(s) 238
BstBAI YACGTR 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 306
BstENI CCTNNNNNAGG 1 cut(s) 54
BstKTI GATC 3 cut(s) 135, 225, 272
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 3 cut(s) 132, 222, 269
BstMCI CGRYCG 1 cut(s) 240
BstNI CCWGG 2 cut(s) 193, 217
BstNSI RCATGY 1 cut(s) 16
BstSCI CCNGG 3 cut(s) 24, 191, 215
BstSFI CTRYAG 1 cut(s) 207
BstSLI GKGCMC 1 cut(s) 216
BstSNI TACGTA 1 cut(s) 167
BstV1I GCAGC 3 cut(s) 193, 214, 229
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 306
CaiI CAGNNNCTG 1 cut(s) 284
Csp6I GTAC 2 cut(s) 159, 168
CviAII CATG 2 cut(s) 13, 82
CviJI RGCY 4 cut(s) 107, 189, 281, 339
CviKI_1 RGCY 4 cut(s) 107, 189, 281, 339
CviQI GTAC 2 cut(s) 159, 168
DpnI GATC 3 cut(s) 134, 224, 271
DpnII GATC 3 cut(s) 132, 222, 269
Eco105I TACGTA 1 cut(s) 167
EcoNI CCTNNNNNAGG 1 cut(s) 54
EcoRII CCWGG 2 cut(s) 191, 215
FaeI CATG 2 cut(s) 16, 85
FaiI YATR 8 cut(s) 14, 83, 141, 143, 149, 314, 331, 333
FaqI GGGAC 2 cut(s) 185, 260
FatI CATG 2 cut(s) 12, 81
FauI CCCGC 1 cut(s) 211
FbaI TGATCA 1 cut(s) 132
Fnu4HI GCNGC 3 cut(s) 207, 228, 243
Fsp4HI GCNGC 3 cut(s) 207, 228, 243
GluI GCNGC 3 cut(s) 207, 228, 243
GsaI CCCAGC 2 cut(s) 111, 281
GsuI CTGGAG 1 cut(s) 175
HapII CCGG 1 cut(s) 25
Hin1II CATG 2 cut(s) 16, 85
HinfI GANTC 1 cut(s) 198
HpaII CCGG 1 cut(s) 25
HpyAV CCTTC 1 cut(s) 50
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 2 cut(s) 209, 344
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 2 cut(s) 16, 85
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 3 cut(s) 132, 222, 269
LmnI GCTCC 2 cut(s) 49, 194
Lsp1109I GCAGC 3 cut(s) 193, 214, 229
MaeII ACGT 1 cut(s) 166
MalI GATC 3 cut(s) 134, 224, 271
MboI GATC 3 cut(s) 132, 222, 269
MboII GAAGA 4 cut(s) 44, 98, 232, 265
MfeI CAATTG 1 cut(s) 345
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 2 cut(s) 99, 345
MlyI GAGTC 1 cut(s) 207
MnlI CCTC 1 cut(s) 319
MseI TTAA 1 cut(s) 352
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 1 cut(s) 25
MspR9I CCNGG 3 cut(s) 26, 193, 217
MunI CAATTG 1 cut(s) 345
MvaI CCWGG 2 cut(s) 193, 217
NciI CCSGG 1 cut(s) 26
NdeII GATC 3 cut(s) 132, 222, 269
NlaIII CATG 2 cut(s) 16, 85
NlaIV GGNNCC 2 cut(s) 190, 297
NspI RCATGY 1 cut(s) 16
PciI ACATGT 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 163
PflMI CCANNNNNTGG 1 cut(s) 284
PfoI TCCNGGA 1 cut(s) 191
PkrI GCNGC 3 cut(s) 208, 229, 244
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
Ppu21I YACGTR 1 cut(s) 167
PscI ACATGT 1 cut(s) 12
Psp6I CCWGG 2 cut(s) 191, 215
PspFI CCCAGC 2 cut(s) 107, 277
PspGI CCWGG 2 cut(s) 191, 215
PspN4I GGNNCC 2 cut(s) 190, 297
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 284
RsaI GTAC 2 cut(s) 160, 169
RsaNI GTAC 2 cut(s) 159, 168
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 1 cut(s) 352
SatI GCNGC 3 cut(s) 207, 228, 243
Sau3AI GATC 3 cut(s) 132, 222, 269
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 3 cut(s) 26, 193, 217
SduI GDGCHC 1 cut(s) 216
SetI ASST 5 cut(s) 52, 61, 109, 169, 283
SfcI CTRYAG 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 17
SnaBI TACGTA 1 cut(s) 167
Sse9I AATT 2 cut(s) 99, 345
SsiI CCGC 3 cut(s) 204, 245, 302
StyD4I CCNGG 3 cut(s) 24, 191, 215
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 1 cut(s) 169
TaqI TCGA 2 cut(s) 30, 268
TasI AATT 2 cut(s) 99, 345
Tru1I TTAA 1 cut(s) 352
Tru9I TTAA 1 cut(s) 352
TscAI CASTG 1 cut(s) 216
TseI GCWGC 3 cut(s) 206, 227, 242
TspDTI ATGAA 3 cut(s) 69, 105, 272
TspRI CASTG 1 cut(s) 216
Van91I CCANNNNNTGG 1 cut(s) 284
XagI CCTNNNNNAGG 1 cut(s) 54
XceI RCATGY 1 cut(s) 16
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.