Rw3G003590

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000613
Physical Location & Seq
Forward (+)
3052583 .. 3054418
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003590.1

Sequence Viewer

Length: 1146 bp
ATGGCCAGAAGAAAAATAATGAAGTTGGTCAAGGGGACAACTCTTGCATTTGTATATGAAGGAGGCATCATGATGGGCACAGATTCGAGGACCACATCTTGCTATATACATAATGGCAATATAAAAGGAAATATAATGACAGACAAGGAAGAAAAAATTAAGAAGCTATCTTCCCACATCAACTTATTTTGGACTATGATGGGGCATAAAGATGATTGGGAGAAAATGTACTGTTATGTGAGTCGGAATTCACCTAGATCAATTGACGAAGCGGTAAAAATAATAAAAGAGTATTTACATCGTTACATTAAAAATCAGAACGCGGTGTTGGATGAATGCAAAAAGATTTACAACGAGGACCATACGTTTGGCACCATTATTGGTGGATACGACCCCGATAAGGGCTTTCAAGTTCGTAGTGTAACTCTTCTAGGCTATGATATTTTCGAAAGTGACCCTGATGCAACAATTTTAACACTAGGGTCCGGTGAGGATGATGCGTACGAAATTTTGAACCCAGCAAACACATATTATGGTGAACACATACGCATGACTAGAGAACGTGCATATTACTTGGCTTTACATGCCTTATTTTATGCATCTTTCTGGGATAAGTATTCTGGTGGAACTCTAAATGTGATACATGTGTCTAAAACCACGTCTTCTCTCGAGACCCACAATGCTTTGCAAGTCTACCCCAAATTTTATAACCTTATGCCTAGAGATGCAAGTGATGTTCATAGCAGAACATTTTTCGTGCTTTATCCTACGAATTCCTATTCCAAACTTGTGTGGGAAGGTGATGGTCTTGAAGACCTCATCCTCAGAGTCTGGCCAAAGGGAGGTGTAGACTTACAGCAGCACCTAGAAGCAAGCCATTTTGTAGCAAAGAAGTACGATTTCTACTTTCACCGTTTCGTGTTCAAGCAAGTAAACAAGGCTATACAAGCATACAATACTATCACTACCATAGCTCCTCCCCTACGTCATAACGCTATAGAAGATTATTTCCAAGGCAATTTTAAACGTTCTGGCAGCCAAAAGAAGCCCGTTTATATTGGATGGAGCTCAGAAGACTTGTTGAAACGAATCTGTAACATGAAGGATGCTACTAATTCATGGTACTGCAAGTATTTGACCTTCTAA

Protein Analysis

381

Amino Acids

44.2

Weight (kDa)

8.65

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 12 - 215 4.5e-08 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
AccB1I GGYRCC 1 cut(s) 371
AccI GTMKAC 2 cut(s) 693, 849
AccII CGCG 1 cut(s) 323
AciI CCGC 2 cut(s) 272, 323
AclI AACGTT 1 cut(s) 1027
AcoI YGGCCR 2 cut(s) 3, 833
AcsI RAATTY 4 cut(s) 247, 507, 701, 772
AfaI GTAC 4 cut(s) 230, 503, 896, 1124
AfiI CCNNNNNNNGG 3 cut(s) 400, 401, 842
AflIII ACRYGT 1 cut(s) 643
AgsI TTSAA 5 cut(s) 410, 514, 812, 925, 1084
AjiI CACGTC 1 cut(s) 660
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 3 cut(s) 166, 974, 1068
AluI AGCT 3 cut(s) 166, 974, 1068
Alw21I GWGCWC 1 cut(s) 1070
Alw26I GTCTC 1 cut(s) 665
AlwNI CAGNNNCTG 1 cut(s) 831
Ama87I CYCGRG 1 cut(s) 668
AoxI GGCC 2 cut(s) 3, 833
ApeKI GCWGC 2 cut(s) 859, 1035
ApoI RAATTY 4 cut(s) 247, 507, 701, 772
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspS9I GGNCC 3 cut(s) 90, 358, 483
AsuHPI GGTGA 5 cut(s) 243, 500, 548, 812, 902
AsuII TTCGAA 1 cut(s) 447
AvaI CYCGRG 1 cut(s) 668
AvaII GGWCC 3 cut(s) 90, 358, 483
BaeGI GKGCMC 1 cut(s) 80
BaeI ACNNNNGTAYC 2 cut(s) 632, 665
BalI TGGCCA 2 cut(s) 5, 835
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 1070
BarI GAAGNNNNNNTAC 1 cut(s) 1124
BbsI GAAGAC 3 cut(s) 654, 819, 1080
Bbv12I GWGCWC 1 cut(s) 1070
BbvI GCAGC 2 cut(s) 871, 1047
BccI CCATC 4 cut(s) 67, 193, 797, 1056
BciVI GTATCC 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 665
BfaI CTAG 6 cut(s) 255, 431, 479, 555, 720, 866
BfmI CTRYAG 1 cut(s) 996
BfuI GTATCC 1 cut(s) 380
BisI GCNGC 2 cut(s) 860, 1036
BlsI GCNGC 2 cut(s) 861, 1037
Bme18I GGWCC 3 cut(s) 90, 358, 483
BmeT110I CYCGRG 1 cut(s) 668
BmgBI CACGTC 1 cut(s) 660
BmgT120I GGNCC 3 cut(s) 90, 358, 483
BmiI GGNNCC 2 cut(s) 373, 484
BmsI GCATC 6 cut(s) 75, 451, 487, 608, 715, 1096
BpiI GAAGAC 3 cut(s) 654, 819, 1080
Bpu14I TTCGAA 1 cut(s) 447
BsaI GGTCTC 1 cut(s) 665
BsaJI CCNNGG 1 cut(s) 1012
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 958, 988
Bsc4I CCNNNNNNNGG 3 cut(s) 400, 401, 842
BseDI CCNNGG 1 cut(s) 1012
BseGI GGATG 5 cut(s) 337, 499, 819, 1067, 1111
BseLI CCNNNNNNNGG 3 cut(s) 400, 401, 842
BseMII CTCAG 2 cut(s) 838, 1083
BseRI GAGGAG 1 cut(s) 966
BseSI GKGCMC 1 cut(s) 80
BseXI GCAGC 2 cut(s) 871, 1047
BseYI CCCAGC 1 cut(s) 517
Bsh1236I CGCG 1 cut(s) 323
BshFI GGCC 2 cut(s) 5, 835
BshNI GGYRCC 1 cut(s) 371
BsiHKAI GWGCWC 1 cut(s) 1070
BsiHKCI CYCGRG 1 cut(s) 668
BsiSI CCGG 1 cut(s) 486
BsiWI CGTACG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 49
BslI CCNNNNNNNGG 3 cut(s) 400, 401, 842
BsmAI GTCTC 1 cut(s) 665
BsmFI GGGAC 1 cut(s) 49
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 2 cut(s) 5, 835
Bso31I GGTCTC 1 cut(s) 665
BsoBI CYCGRG 1 cut(s) 668
Bsp119I TTCGAA 1 cut(s) 447
Bsp1286I GDGCHC 2 cut(s) 80, 1070
Bsp143I GATC 1 cut(s) 257
BspACI CCGC 2 cut(s) 272, 323
BspANI GGCC 2 cut(s) 5, 835
BspCNI CTCAG 2 cut(s) 837, 1082
BspFNI CGCG 1 cut(s) 323
BspHI TCATGA 1 cut(s) 69
BspLI GGNNCC 2 cut(s) 373, 484
BspT104I TTCGAA 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 371
BspTNI GGTCTC 1 cut(s) 665
BssECI CCNNGG 1 cut(s) 1012
BssMI GATC 1 cut(s) 257
BssT1I CCWWGG 1 cut(s) 1012
Bst4CI ACNGT 2 cut(s) 233, 914
Bst6I CTCTTC 1 cut(s) 432
BstBI TTCGAA 1 cut(s) 447
BstC8I GCNNGC 1 cut(s) 874
BstDEI CTNAG 2 cut(s) 824, 1069
BstF5I GGATG 5 cut(s) 337, 499, 819, 1067, 1111
BstFNI CGCG 1 cut(s) 323
BstKTI GATC 1 cut(s) 260
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 1 cut(s) 257
BstMWI GCNNNNNNNGC 2 cut(s) 584, 947
BstNSI RCATGY 2 cut(s) 587, 647
BstSFI CTRYAG 1 cut(s) 996
BstSLI GKGCMC 1 cut(s) 80
BstUI CGCG 1 cut(s) 323
BstV1I GCAGC 2 cut(s) 871, 1047
BstV2I GAAGAC 3 cut(s) 654, 819, 1080
BstXI CCANNNNNNTGG 1 cut(s) 368
BsuI GTATCC 1 cut(s) 380
BsuRI GGCC 2 cut(s) 5, 835
BtrI CACGTC 1 cut(s) 660
BtsCI GGATG 5 cut(s) 337, 499, 819, 1067, 1111
Cac8I GCNNGC 1 cut(s) 874
CaiI CAGNNNCTG 1 cut(s) 831
CciI TCATGA 1 cut(s) 69
Cfr13I GGNCC 3 cut(s) 90, 358, 483
Csp6I GTAC 4 cut(s) 229, 502, 895, 1123
CviAII CATG 6 cut(s) 70, 550, 584, 644, 1099, 1119
CviQI GTAC 4 cut(s) 229, 502, 895, 1123
DdeI CTNAG 2 cut(s) 824, 1069
DpnI GATC 1 cut(s) 259
DpnII GATC 1 cut(s) 257
DraI TTTAAA 1 cut(s) 1024
EaeI YGGCCR 2 cut(s) 3, 833
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
Ecl136II GAGCTC 1 cut(s) 1068
Eco130I CCWWGG 1 cut(s) 1012
Eco24I GRGCYC 1 cut(s) 1070
Eco31I GGTCTC 1 cut(s) 665
Eco47I GGWCC 3 cut(s) 90, 358, 483
Eco53kI GAGCTC 1 cut(s) 1068
Eco88I CYCGRG 1 cut(s) 668
EcoICRI GAGCTC 1 cut(s) 1068
EcoRI GAATTC 2 cut(s) 247, 772
EcoT14I CCWWGG 1 cut(s) 1012
EcoT22I ATGCAT 1 cut(s) 601
EcoT38I GRGCYC 1 cut(s) 1070
ErhI CCWWGG 1 cut(s) 1012
FaeI CATG 6 cut(s) 73, 553, 587, 647, 1102, 1122
FaqI GGGAC 1 cut(s) 49
FatI CATG 6 cut(s) 69, 549, 583, 643, 1098, 1118
FblI GTMKAC 2 cut(s) 693, 849
Fnu4HI GCNGC 2 cut(s) 860, 1036
FokI GGATG 5 cut(s) 344, 506, 806, 1074, 1118
FriOI GRGCYC 1 cut(s) 1070
Fsp4HI GCNGC 2 cut(s) 860, 1036
FspBI CTAG 6 cut(s) 255, 431, 479, 555, 720, 866
GluI GCNGC 2 cut(s) 860, 1036
GsaI CCCAGC 1 cut(s) 521
HaeIII GGCC 2 cut(s) 5, 835
HapII CCGG 1 cut(s) 486
Hin1II CATG 6 cut(s) 73, 553, 587, 647, 1102, 1122
HinfI GANTC 4 cut(s) 83, 241, 828, 1089
HpaII CCGG 1 cut(s) 486
HphI GGTGA 5 cut(s) 243, 500, 548, 812, 902
Hpy166II GTNNAC 4 cut(s) 539, 694, 850, 934
Hpy188I TCNGA 4 cut(s) 246, 318, 827, 1072
Hpy188III TCNNGA 4 cut(s) 70, 668, 670, 809
Hpy8I GTNNAC 4 cut(s) 539, 694, 850, 934
HpyAV CCTTC 3 cut(s) 53, 791, 1096
HpyCH4III ACNGT 2 cut(s) 233, 914
HpyCH4IV ACGT 5 cut(s) 365, 562, 659, 985, 1027
HpyCH4V TGCA 8 cut(s) 47, 339, 464, 566, 599, 688, 728, 1128
HpyF10VI GCNNNNNNNGC 2 cut(s) 584, 947
HpyF3I CTNAG 2 cut(s) 824, 1069
HpySE526I ACGT 5 cut(s) 365, 562, 659, 985, 1027
Hsp92II CATG 6 cut(s) 73, 553, 587, 647, 1102, 1122
Kzo9I GATC 1 cut(s) 257
LmnI GCTCC 2 cut(s) 979, 1065
LpnPI CCDG 8 cut(s) 19, 471, 499, 531, 592, 606, 817, 1017
Lsp1109I GCAGC 2 cut(s) 871, 1047
LweI GCATC 6 cut(s) 75, 451, 487, 608, 715, 1096
MaeI CTAG 6 cut(s) 255, 431, 479, 555, 720, 866
MaeII ACGT 5 cut(s) 365, 562, 659, 985, 1027
MaeIII GTNAC 4 cut(s) 302, 421, 452, 1094
MalI GATC 1 cut(s) 259
MboI GATC 1 cut(s) 257
MboII GAAGA 8 cut(s) 21, 161, 162, 419, 654, 824, 1013, 1085
MfeI CAATTG 1 cut(s) 261
MhlI GDGCHC 2 cut(s) 80, 1070
MlsI TGGCCA 2 cut(s) 5, 835
MluCI AATT 9 cut(s) 156, 247, 261, 468, 507, 701, 772, 1018, 1114
MluNI TGGCCA 2 cut(s) 5, 835
MlyI GAGTC 2 cut(s) 250, 837
MmeI TCCRAC 2 cut(s) 224, 309
MnlI CCTC 8 cut(s) 56, 81, 349, 484, 827, 833, 836, 987
Mox20I TGGCCA 2 cut(s) 5, 835
Mph1103I ATGCAT 1 cut(s) 601
MscI TGGCCA 2 cut(s) 5, 835
MseI TTAA 4 cut(s) 159, 309, 473, 1023
MslI CAYNNNNRTG 3 cut(s) 71, 210, 548
Msp20I TGGCCA 2 cut(s) 5, 835
MspI CCGG 1 cut(s) 486
MunI CAATTG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 341
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 2 cut(s) 584, 947
NdeII GATC 1 cut(s) 257
NlaIII CATG 6 cut(s) 73, 553, 587, 647, 1102, 1122
NlaIV GGNNCC 2 cut(s) 373, 484
NmuCI GTSAC 1 cut(s) 452
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 2 cut(s) 587, 647
NspV TTCGAA 1 cut(s) 447
PaeR7I CTCGAG 1 cut(s) 668
PagI TCATGA 1 cut(s) 69
PciI ACATGT 1 cut(s) 643
PctI GAATGC 1 cut(s) 341
PfeI GAWTC 2 cut(s) 83, 1089
Pfl23II CGTACG 1 cut(s) 501
PkrI GCNGC 2 cut(s) 861, 1037
PleI GAGTC 2 cut(s) 249, 836
PpsI GAGTC 2 cut(s) 249, 836
PscI ACATGT 1 cut(s) 643
PsiI TTATAA 1 cut(s) 708
Psp124BI GAGCTC 1 cut(s) 1070
Psp1406I AACGTT 1 cut(s) 1027
PspFI CCCAGC 1 cut(s) 517
PspLI CGTACG 1 cut(s) 501
PspN4I GGNNCC 2 cut(s) 373, 484
PspPI GGNCC 3 cut(s) 90, 358, 483
PstNI CAGNNNCTG 1 cut(s) 831
RsaI GTAC 4 cut(s) 230, 503, 896, 1124
RsaNI GTAC 4 cut(s) 229, 502, 895, 1123
RseI CAYNNNNRTG 3 cut(s) 71, 210, 548
SacI GAGCTC 1 cut(s) 1070
SaqAI TTAA 4 cut(s) 159, 309, 473, 1023
SatI GCNGC 2 cut(s) 860, 1036
Sau3AI GATC 1 cut(s) 257
Sau96I GGNCC 3 cut(s) 90, 358, 483
SchI GAGTC 2 cut(s) 250, 837
SduI GDGCHC 2 cut(s) 80, 1070
SfaNI GCATC 6 cut(s) 75, 451, 487, 608, 715, 1096
SfcI CTRYAG 1 cut(s) 996
Sfr274I CTCGAG 1 cut(s) 668
SfuI TTCGAA 1 cut(s) 447
SinI GGWCC 3 cut(s) 90, 358, 483
SlaI CTCGAG 1 cut(s) 668
SmiMI CAYNNNNRTG 3 cut(s) 71, 210, 548
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
Sse9I AATT 9 cut(s) 156, 247, 261, 468, 507, 701, 772, 1018, 1114
SsiI CCGC 2 cut(s) 272, 323
SspMI CTAG 6 cut(s) 255, 431, 479, 555, 720, 866
SstI GAGCTC 1 cut(s) 1070
StyI CCWWGG 1 cut(s) 1012
TaaI ACNGT 2 cut(s) 233, 914
TaiI ACGT 5 cut(s) 368, 565, 662, 988, 1030
TaqI TCGA 3 cut(s) 86, 447, 669
TasI AATT 9 cut(s) 156, 247, 261, 468, 507, 701, 772, 1018, 1114
TatI WGTACW 1 cut(s) 228
TfiI GAWTC 2 cut(s) 83, 1089
Tru1I TTAA 4 cut(s) 159, 309, 473, 1023
Tru9I TTAA 4 cut(s) 159, 309, 473, 1023
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 2 cut(s) 859, 1035
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 6 cut(s) 35, 72, 348, 728, 1107, 1115
VpaK11BI GGWCC 3 cut(s) 90, 358, 483
XapI RAATTY 4 cut(s) 247, 507, 701, 772
XceI RCATGY 2 cut(s) 587, 647
XhoI CTCGAG 1 cut(s) 668
XmiI GTMKAC 2 cut(s) 693, 849
XspI CTAG 6 cut(s) 255, 431, 479, 555, 720, 866
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.