AT2G30660

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000464
Physical Location & Seq
Reverse (-)
13058250 .. 13061675
3426 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G30660.2

Sequence Viewer

Length: 1137 bp
ATGGCATCTCAATCTCAGGTTTTGGTGGAAGAGAAATCAAGTGTTAGAATTTTGACATTAAACCGACCCAAGCAGCTTAATGCTCTGTGCTTCAACATGATCTCTCGGTTGCTGCAACTATTCCGTGCATATGAGGAAGACCCTAGTGTTAAACTTGTCATCCTAAAGGGTCAAGGAAGAGCCTTTTGTGCCGGTGGCGACGTTCCACCTGTTGTTCAAAACATGGTACAAGGTAAATGGAGACTTGGTGCCGATTTCTTCAGAGATCAATACACGCTCAATTATGTTATGGCCACGTATAGCAAACCTCAGGTTTCAATTTTGAATGGTATTGTCATGGGAGCCGGTGCTGGTGTCTCCATCCATGGTCGATTTCGCATTGCAACTGAGAACACGGTTTTTGCCATGCCGGAGACATCTCTGGGGCTCTTTCCAGATGTAGGCGCCTCCTATTTCCTGTCACGGCTCCCTGGATTTTTTGGAGAGTATGTTGGCCTTACAGGAGCTAGGTTAGATGGCGCTGAATTGCTTGCGTGTGGTCTTGCAACTCATTTTGTTCCTTCAACGAGGTTGACAGCTCTAGAAACAGATCTTTGCAAAGTTGGGTCAAGTGATCCAAGCTTTGTCTCAACAATTCTTGATGCATACACTCAGCATCCGCACCTTAAACAGAAGAGTGCTTACCACAGGTTAGATGTTATCGATAGGTGCTTCTCGAAGAGAACAATGGAAGAAATTATATCTGCACTTGAGAGAGAGACCACTCAAGAACTAGATGATTGGAGCTTAACTACGATTCGAGCTTTGAAGAAGTCTTCACCCTCAAGCCTTAAAATATCTCTTAGATCGATAAGAGAAGGAAGATTGCAAGGGGTAGGACACTGTCTTACCCGCGAGTATAGAATGGTGTGTCATGTGATGAAGGGAGATTTAAGCAAAGACTTGGTGGAGGGGTGCAGAGCCATATTGATAGACAAAGATAGGAATCCAAAGTGGGAGCCAAGGCGATTGGAGGACATGAAGGATAGCATGGTAGATCAGTTCTTTGAGAGAGTGGAAGAAGAGGAGCGATGGGAGGATCTTAAGCTTTCACCAAGGAACAACTTGCATGCTTTAAGAATTGCAGCAAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200
Pfam Domains
Protein Families
ECH_2

Protein Analysis

378

Amino Acids

42.57

Weight (kDa)

8.54

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 1 - 317 2.8e-119 Enoyl-CoA hydratase/isomerase
ECH_1 PF00378 2 - 208 5.4e-24 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000464)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30650 AT2G30650 AT2G30660 AT2G30660 AT5G65940 AT5G65940 AT5G65940 AT5G65940
fragaria_vesca FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g09160 FvH4_7g15030 FvH4_7g18470
malus_domestica MD00G1098500.v1.1 MD02G1229900.v1.1 MD07G1169300.v1.1 MD07G1169400.v1.1
prunus_persica Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G207200_v2.0.a1 Prupe.2G207400_v2.0.a1 Prupe.2G207600_v2.0.a1 Prupe.2G207700_v2.0.a1 Prupe.2G207800_v2.0.a1
pyrus_communis pycom02g19850 pycom07g06680 pycom07g16310
rosa_chinensis RchiOBHm_Chr1g0341301 RchiOBHm_Chr1g0345221 RchiOBHm_Chr1g0357091 RchiOBHm_Chr1g0357121 RchiOBHm_Chr1g0357151
rosa_laevigata RLG00000027659 RLG00000028049 RLG00000028052 RLG00000029082 RLG00000029626
rosa_multiflora Rmu_sc0000117.1_g000042 Rmu_sc0000166.1_g000050 Rmu_sc0004092.1_g000048 Rmu_sc0005967.1_g000013 Rmu_sc0006896.1_g000007 Rmu_sc0011699.1_g000007 Rmu_sc0013980.1_g000002 Rmu_sc0021527.1_g000001 Rmu_sc0024834.1_g000001 Rmu_ssc0000009.1_g000009
rosa_roxburghii Rroxscaffold_4G00294310 Rroxscaffold_4G00299050 Rroxscaffold_4G00311940
rosa_rugosa Rorug01G0157800.1 Rorug01G0254200 Rorug01G0254300.1 Rorug01G0296600.1 Rorug01G0296800 Rorug01G0296900 Rorug06G0065700
rosa_samantha Rh1AG129700 Rh1AG162000 Rh1AG172400 Rh1AG268500 Rh1AG268600 Rh1AG305700 Rh1BG140400 Rh1BG236100 Rh1BG236200 Rh1BG269000 Rh1BG269100 Rh1CG123500 Rh1CG151300 Rh1CG160500 Rh1CG252100 Rh1CG252300 Rh1CG286900 Rh1DG172700 Rh1DG263300 Rh1DG263400 Rh1DG299000 Rh1DG299100
rosa_wichuraiana Rw0G018900 Rw0G022950 Rw1G013330 Rw1G014380 Rw1G023680 Rw1G023700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 248, 443
AccII CGCG 1 cut(s) 894
AciI CCGC 2 cut(s) 659, 892
AclWI GGATC 2 cut(s) 608, 1086
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 1 cut(s) 48
AcuI CTGAAG 1 cut(s) 244
AcyI GRCGYC 1 cut(s) 444
AfaI GTAC 1 cut(s) 228
AfiI CCNNNNNNNGG 1 cut(s) 440
AflII CTTAAG 1 cut(s) 1082
AgsI TTSAA 6 cut(s) 94, 218, 318, 325, 564, 808
AjnI CCWGG 1 cut(s) 469
AluBI AGCT 7 cut(s) 76, 506, 578, 621, 786, 803, 1087
AluI AGCT 7 cut(s) 76, 506, 578, 621, 786, 803, 1087
Alw26I GTCTC 5 cut(s) 235, 361, 407, 631, 752
AlwI GGATC 2 cut(s) 608, 1086
AoxI GGCC 2 cut(s) 291, 493
ApeKI GCWGC 3 cut(s) 73, 112, 1124
ApoI RAATTY 1 cut(s) 48
AspLEI GCGC 2 cut(s) 446, 521
AsuHPI GGTGA 2 cut(s) 810, 1083
AxyI CCTNAGG 1 cut(s) 309
BalI TGGCCA 1 cut(s) 293
BanI GGYRCC 2 cut(s) 248, 443
BanII GRGCYC 1 cut(s) 429
BarI GAAGNNNNNNTAC 2 cut(s) 665, 697
BbsI GAAGAC 2 cut(s) 144, 807
BbvI GCAGC 2 cut(s) 85, 99
BccI CCATC 3 cut(s) 368, 509, 1065
BceAI ACGGC 1 cut(s) 479
BciT130I CCWGG 1 cut(s) 471
BcoDI GTCTC 5 cut(s) 235, 361, 407, 631, 752
BfaI CTAG 4 cut(s) 144, 507, 581, 773
BfoI RGCGCY 2 cut(s) 447, 522
BfrI CTTAAG 1 cut(s) 1082
BglII AGATCT 1 cut(s) 589
BisI GCNGC 3 cut(s) 74, 113, 1125
BlsI GCNGC 3 cut(s) 75, 114, 1126
Bme1390I CCNGG 1 cut(s) 471
BmiI GGNNCC 5 cut(s) 250, 343, 445, 467, 999
BmrFI CCNGG 1 cut(s) 471
BmsI GCATC 3 cut(s) 14, 631, 664
BpiI GAAGAC 2 cut(s) 144, 807
BpuEI CTTGAG 3 cut(s) 750, 770, 808
Bsa29I ATCGAT 2 cut(s) 702, 848
BsaAI YACGTR 1 cut(s) 297
BsaBI GATNNNNATC 1 cut(s) 984
BsaHI GRCGYC 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 752
BsaJI CCNNGG 4 cut(s) 364, 469, 1001, 1094
BsaXI ACNNNNNCTCC 4 cut(s) 232, 262, 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse118I RCCGGY 2 cut(s) 191, 344
Bse21I CCTNAGG 1 cut(s) 309
Bse3DI GCAATG 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 984
BseBI CCWGG 1 cut(s) 471
BseCI ATCGAT 2 cut(s) 702, 848
BseDI CCNNGG 4 cut(s) 364, 469, 1001, 1094
BseGI GGATG 3 cut(s) 159, 360, 655
BseJI GATNNNNATC 1 cut(s) 984
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 4 cut(s) 29, 323, 378, 665
BseRI GAGGAG 1 cut(s) 1079
BseXI GCAGC 2 cut(s) 85, 99
BsgI GTGCAG 2 cut(s) 729, 976
Bsh1236I CGCG 1 cut(s) 894
BshFI GGCC 2 cut(s) 293, 495
BshNI GGYRCC 2 cut(s) 248, 443
BshVI ATCGAT 2 cut(s) 702, 848
BsiSI CCGG 3 cut(s) 192, 345, 410
BslI CCNNNNNNNGG 1 cut(s) 440
BsmAI GTCTC 5 cut(s) 235, 361, 407, 631, 752
BsnI GGCC 2 cut(s) 293, 495
Bso31I GGTCTC 1 cut(s) 752
Bsp1286I GDGCHC 1 cut(s) 429
Bsp143I GATC 7 cut(s) 99, 265, 589, 613, 845, 1036, 1078
Bsp19I CCATGG 1 cut(s) 364
BspACI CCGC 2 cut(s) 659, 892
BspANI GGCC 2 cut(s) 293, 495
BspCNI CTCAG 4 cut(s) 28, 322, 379, 664
BspDI ATCGAT 2 cut(s) 702, 848
BspFNI CGCG 1 cut(s) 894
BspLI GGNNCC 5 cut(s) 250, 343, 445, 467, 999
BspPI GGATC 2 cut(s) 608, 1086
BspQI GCTCTTC 1 cut(s) 172
BspT107I GGYRCC 2 cut(s) 248, 443
BspTI CTTAAG 1 cut(s) 1082
BspTNI GGTCTC 1 cut(s) 752
BsrDI GCAATG 1 cut(s) 378
BsrFI RCCGGY 2 cut(s) 191, 344
BssAI RCCGGY 2 cut(s) 191, 344
BssECI CCNNGG 4 cut(s) 364, 469, 1001, 1094
BssMI GATC 7 cut(s) 99, 265, 589, 613, 845, 1036, 1078
BssNI GRCGYC 1 cut(s) 444
BssT1I CCWWGG 3 cut(s) 364, 1001, 1094
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 2 cut(s) 397, 884
Bst6I CTCTTC 5 cut(s) 24, 172, 668, 713, 1056
BstACI GRCGYC 1 cut(s) 444
BstAFI CTTAAG 1 cut(s) 1082
BstBAI YACGTR 1 cut(s) 297
BstC8I GCNNGC 2 cut(s) 531, 1110
BstDEI CTNAG 5 cut(s) 15, 309, 387, 651, 842
BstDSI CCRYGG 1 cut(s) 364
BstF5I GGATG 3 cut(s) 159, 360, 655
BstFNI CGCG 1 cut(s) 894
BstH2I RGCGCY 2 cut(s) 447, 522
BstHHI GCGC 2 cut(s) 446, 521
BstKTI GATC 7 cut(s) 102, 268, 592, 616, 848, 1039, 1081
BstMAI GTCTC 5 cut(s) 235, 361, 407, 631, 752
BstMBI GATC 7 cut(s) 99, 265, 589, 613, 845, 1036, 1078
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNI CCWGG 1 cut(s) 471
BstNSI RCATGY 1 cut(s) 1112
BstSCI CCNGG 1 cut(s) 469
BstUI CGCG 1 cut(s) 894
BstV1I GCAGC 2 cut(s) 85, 99
BstV2I GAAGAC 2 cut(s) 144, 807
BstX2I RGATCY 2 cut(s) 589, 1078
BstYI RGATCY 2 cut(s) 589, 1078
Bsu15I ATCGAT 2 cut(s) 702, 848
Bsu36I CCTNAGG 1 cut(s) 309
BsuRI GGCC 2 cut(s) 293, 495
BsuTUI ATCGAT 2 cut(s) 702, 848
BtgI CCRYGG 1 cut(s) 364
BtgZI GCGATG 1 cut(s) 1084
BtsCI GGATG 3 cut(s) 159, 360, 655
BtsIMutI CAGTG 1 cut(s) 880
Cac8I GCNNGC 2 cut(s) 531, 1110
CfoI GCGC 2 cut(s) 446, 521
Cfr10I RCCGGY 2 cut(s) 191, 344
ClaI ATCGAT 2 cut(s) 702, 848
Csp6I GTAC 1 cut(s) 227
CviAII CATG 9 cut(s) 97, 223, 337, 365, 406, 914, 1018, 1030, 1109
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 5 cut(s) 15, 309, 387, 651, 842
DinI GGCGCC 1 cut(s) 445
DpnI GATC 7 cut(s) 101, 267, 591, 615, 847, 1038, 1080
DpnII GATC 7 cut(s) 99, 265, 589, 613, 845, 1036, 1078
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 5 cut(s) 24, 172, 668, 713, 1056
EarI CTCTTC 5 cut(s) 24, 172, 668, 713, 1056
Eco130I CCWWGG 3 cut(s) 364, 1001, 1094
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 1 cut(s) 752
Eco57I CTGAAG 1 cut(s) 244
Eco81I CCTNAGG 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 469
EcoT14I CCWWGG 3 cut(s) 364, 1001, 1094
EcoT22I ATGCAT 1 cut(s) 646
EcoT38I GRGCYC 1 cut(s) 429
EgeI GGCGCC 1 cut(s) 445
EheI GGCGCC 1 cut(s) 445
ErhI CCWWGG 3 cut(s) 364, 1001, 1094
FaeI CATG 9 cut(s) 100, 226, 340, 368, 409, 917, 1021, 1033, 1112
FatI CATG 9 cut(s) 96, 222, 336, 364, 405, 913, 1017, 1029, 1108
FauI CCCGC 1 cut(s) 899
FauNDI CATATG 1 cut(s) 130
Fnu4HI GCNGC 3 cut(s) 74, 113, 1125
FokI GGATG 3 cut(s) 146, 347, 642
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 3 cut(s) 74, 113, 1125
FspBI CTAG 4 cut(s) 144, 507, 581, 773
GlaI GCGC 2 cut(s) 445, 520
GluI GCNGC 3 cut(s) 74, 113, 1125
HaeII RGCGCY 2 cut(s) 447, 522
HaeIII GGCC 2 cut(s) 293, 495
HapII CCGG 3 cut(s) 192, 345, 410
HhaI GCGC 2 cut(s) 446, 521
Hin1I GRCGYC 1 cut(s) 444
Hin1II CATG 9 cut(s) 100, 226, 340, 368, 409, 917, 1021, 1033, 1112
Hin6I GCGC 2 cut(s) 444, 519
HinP1I GCGC 2 cut(s) 444, 519
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HindIII AAGCTT 2 cut(s) 619, 1085
HinfI GANTC 2 cut(s) 796, 985
HpaII CCGG 3 cut(s) 192, 345, 410
HphI GGTGA 2 cut(s) 810, 1083
Hpy166II GTNNAC 1 cut(s) 573
Hpy188I TCNGA 1 cut(s) 263
Hpy188III TCNNGA 5 cut(s) 434, 581, 638, 715, 767
Hpy8I GTNNAC 1 cut(s) 573
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 4 cut(s) 570, 851, 916, 1015
HpyCH4III ACNGT 2 cut(s) 397, 884
HpyCH4IV ACGT 2 cut(s) 201, 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 5 cut(s) 15, 309, 387, 651, 842
HpySE526I ACGT 2 cut(s) 201, 296
Hsp92I GRCGYC 1 cut(s) 444
Hsp92II CATG 9 cut(s) 100, 226, 340, 368, 409, 917, 1021, 1033, 1112
HspAI GCGC 2 cut(s) 444, 519
KasI GGCGCC 1 cut(s) 443
Kzo9I GATC 7 cut(s) 99, 265, 589, 613, 845, 1036, 1078
LguI GCTCTTC 1 cut(s) 172
LmnI GCTCC 6 cut(s) 341, 471, 503, 783, 997, 1066
Lsp1109I GCAGC 2 cut(s) 85, 99
LweI GCATC 3 cut(s) 14, 631, 664
MaeI CTAG 4 cut(s) 144, 507, 581, 773
MaeII ACGT 2 cut(s) 201, 296
MaeIII GTNAC 1 cut(s) 459
MalI GATC 7 cut(s) 101, 267, 591, 615, 847, 1038, 1080
MboI GATC 7 cut(s) 99, 265, 589, 613, 845, 1036, 1078
MflI RGATCY 2 cut(s) 589, 1078
MhlI GDGCHC 1 cut(s) 429
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 7 cut(s) 48, 280, 318, 524, 633, 735, 1119
MluNI TGGCCA 1 cut(s) 293
Mly113I GGCGCC 1 cut(s) 444
MnlI CCTC 9 cut(s) 127, 318, 457, 561, 832, 943, 1006, 1057, 1069
Mox20I TGGCCA 1 cut(s) 293
Mph1103I ATGCAT 1 cut(s) 646
MscI TGGCCA 1 cut(s) 293
MseI TTAA 9 cut(s) 59, 78, 150, 666, 788, 831, 932, 1083, 1115
Msp20I TGGCCA 1 cut(s) 293
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 3 cut(s) 192, 345, 410
MspR9I CCNGG 1 cut(s) 471
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 1 cut(s) 894
MwoI GCNNNNNNNGC 1 cut(s) 188
NarI GGCGCC 1 cut(s) 444
NcoI CCATGG 1 cut(s) 364
NdeI CATATG 1 cut(s) 130
NdeII GATC 7 cut(s) 99, 265, 589, 613, 845, 1036, 1078
NlaIII CATG 9 cut(s) 100, 226, 340, 368, 409, 917, 1021, 1033, 1112
NlaIV GGNNCC 5 cut(s) 250, 343, 445, 467, 999
NmuCI GTSAC 1 cut(s) 459
NsiI ATGCAT 1 cut(s) 646
NspI RCATGY 1 cut(s) 1112
PaeI GCATGC 1 cut(s) 1112
PciSI GCTCTTC 1 cut(s) 172
PfeI GAWTC 2 cut(s) 796, 985
PflFI GACNNNGTC 1 cut(s) 882
PkrI GCNGC 3 cut(s) 75, 114, 1126
PluTI GGCGCC 1 cut(s) 447
Ppu21I YACGTR 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 469
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 5 cut(s) 250, 343, 445, 467, 999
PsuI RGATCY 2 cut(s) 589, 1078
PsyI GACNNNGTC 1 cut(s) 882
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 172
SaqAI TTAA 9 cut(s) 59, 78, 150, 666, 788, 831, 932, 1083, 1115
SatI GCNGC 3 cut(s) 74, 113, 1125
Sau3AI GATC 7 cut(s) 99, 265, 589, 613, 845, 1036, 1078
ScrFI CCNGG 1 cut(s) 471
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 3 cut(s) 14, 631, 664
SfoI GGCGCC 1 cut(s) 445
SmlI CTYRAG 4 cut(s) 749, 765, 823, 1082
SmoI CTYRAG 4 cut(s) 749, 765, 823, 1082
SphI GCATGC 1 cut(s) 1112
Sse9I AATT 7 cut(s) 48, 280, 318, 524, 633, 735, 1119
SsiI CCGC 2 cut(s) 659, 892
SspDI GGCGCC 1 cut(s) 443
SspMI CTAG 4 cut(s) 144, 507, 581, 773
StyD4I CCNGG 1 cut(s) 469
StyI CCWWGG 3 cut(s) 364, 1001, 1094
TaaI ACNGT 2 cut(s) 397, 884
TaiI ACGT 2 cut(s) 204, 299
TaqI TCGA 5 cut(s) 370, 702, 716, 799, 848
TasI AATT 7 cut(s) 48, 280, 318, 524, 633, 735, 1119
TfiI GAWTC 2 cut(s) 796, 985
Tru1I TTAA 9 cut(s) 59, 78, 150, 666, 788, 831, 932, 1083, 1115
Tru9I TTAA 9 cut(s) 59, 78, 150, 666, 788, 831, 932, 1083, 1115
TscAI CASTG 1 cut(s) 887
TseFI GTSAC 1 cut(s) 459
TseI GCWGC 3 cut(s) 73, 112, 1124
Tsp45I GTSAC 1 cut(s) 459
TspDTI ATGAA 2 cut(s) 935, 1034
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 1 cut(s) 887
Tth111I GACNNNGTC 1 cut(s) 882
Vha464I CTTAAG 1 cut(s) 1082
XapI RAATTY 1 cut(s) 48
XbaI TCTAGA 1 cut(s) 580
XceI RCATGY 1 cut(s) 1112
XspI CTAG 4 cut(s) 144, 507, 581, 773
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.