Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0000464

Physical Location & Seq

Genomic Coordinates Forward (+)

20512957 .. 20520082

Gene Structure 7126 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1098500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1056 bp

ATGGCTTCTTCTATTACCTCTATCCCGCACGAAGGAGATTCGGTTCTGGTACAGAAGAATTTGATTGCGAGGACATTGACATTGAACCGGCCTCGGCAGTTGAATGCCCTTAACTTTGATATGATTTCTCGTCTGTTGGAGCATTTTCTTGCCTTTGAGGATGATGCTAATGTCAAGTTGGTAATTGTCAAGGGGAAAGGAAGAGCCTTTTGTGCTGGTGGAGATGTAGCGGCTGTGGTTTCAATTCTGGACGGGATTGTCATGGGAGGTGGGGCTGGTGCTTCAATACATGGAAGATTCCGTGTTGCAACTGAGAATTCGGTTTTTGCAATGCCAGAAACTGCTTTGGGACTTTTTCCGGATGTAGGTGCCTCTTATTACTTGTCCAGGCTTCCTGGATTCTTCGGAGAATATGTTGGTCTTACAGGTACCAGATTGGATGGTGCTGAAATGCTTGCTTGCAGTCTTGCAACACACTTTGTTCCATCAACGAGATTGTCTTTGTTAGAAGAAGCTTTATACAAAGTTGATTCAAGTGATTTTGCCATTATTCAAGCAGTCATAGATAAATACTCTCACAGTCCAGCTCTGAAGAAGAAAAGTGCGTACTACCGGATGGATGTTATAGACAAGTGCTTTTCTAGAAGAACTGTAGAAGAAATTGTATCTGCCCTTGATGAGGAGGCTCCAAACGGAGTGGATGCTTGGCTAACTTCAACAATTCAAGCTTTAAAGAAGGCATCACCAATGAGTTTGAAGATTTCTTTGAGATCGATTAGAGAAGGAAGACTTCAGGGAGTTGGTCAGTGCCTTGTTCGTGAATATAGAATGGTTTCACATGTTGTGAAAGGAGAAGTCAGCAAGGACTTCAGAGAGGGTTGCAGAGCTTTATTGCTGGACAAGGATAAGAACCCAAAGTGGGAGCCTTCCAAATTGGAGCTCGTCACAAACCATATGGTTGAGCAATACTTCTCTAAGTTGGATGATGAAGGATGGGAAGAATTAAAGCTCCTTGTAAGATCAAATTTGCCTGCAACTGCCATTGCCAAGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003860 GO:0006082 GO:0006520 GO:0006573 GO:0006574 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016054 GO:0016289 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0046395 GO:0050896 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

352

Amino Acids

38.62

Weight (kDa)

7.05

Isoelectric Point (pI)

28.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_2	PF16113	24 - 79	3.1e-18	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	26 - 78	2.2e-10	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	79 - 324	4.6e-90	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	79 - 167	1.8e-09	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30650 AT2G30650 AT2G30660 AT2G30660 AT5G65940 AT5G65940 AT5G65940 AT5G65940
fragaria_vesca	FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g09160 FvH4_7g15030 FvH4_7g18470
malus_domestica	MD00G1098500.v1.1 MD02G1229900.v1.1 MD07G1169300.v1.1 MD07G1169400.v1.1
prunus_persica	Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G207200_v2.0.a1 Prupe.2G207400_v2.0.a1 Prupe.2G207600_v2.0.a1 Prupe.2G207700_v2.0.a1 Prupe.2G207800_v2.0.a1
pyrus_communis	pycom02g19850 pycom07g06680 pycom07g16310
rosa_chinensis	RchiOBHm_Chr1g0341301 RchiOBHm_Chr1g0345221 RchiOBHm_Chr1g0357091 RchiOBHm_Chr1g0357121 RchiOBHm_Chr1g0357151
rosa_laevigata	RLG00000027659 RLG00000028049 RLG00000028052 RLG00000029082 RLG00000029626
rosa_multiflora	Rmu_sc0000117.1_g000042 Rmu_sc0000166.1_g000050 Rmu_sc0004092.1_g000048 Rmu_sc0005967.1_g000013 Rmu_sc0006896.1_g000007 Rmu_sc0011699.1_g000007 Rmu_sc0013980.1_g000002 Rmu_sc0021527.1_g000001 Rmu_sc0024834.1_g000001 Rmu_ssc0000009.1_g000009
rosa_roxburghii	Rroxscaffold_4G00294310 Rroxscaffold_4G00299050 Rroxscaffold_4G00311940
rosa_rugosa	Rorug01G0157800.1 Rorug01G0254200 Rorug01G0254300.1 Rorug01G0296600.1 Rorug01G0296800 Rorug01G0296900 Rorug06G0065700
rosa_samantha	Rh1AG129700 Rh1AG162000 Rh1AG172400 Rh1AG268500 Rh1AG268600 Rh1AG305700 Rh1BG140400 Rh1BG236100 Rh1BG236200 Rh1BG269000 Rh1BG269100 Rh1CG123500 Rh1CG151300 Rh1CG160500 Rh1CG252100 Rh1CG252300 Rh1CG286900 Rh1DG172700 Rh1DG263300 Rh1DG263400 Rh1DG299000 Rh1DG299100
rosa_wichuraiana	Rw0G018900 Rw0G022950 Rw1G013330 Rw1G014380 Rw1G023680 Rw1G023700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	257
Acc65I	GGTACC	1 cut(s)	428
AccB1I	GGYRCC	2 cut(s)	368, 428
AccIII	TCCGGA	1 cut(s)	358
AciI	CCGC	2 cut(s)	26, 230
AcsI	RAATTY	3 cut(s)	58, 316, 1024
AcuI	CTGAAG	3 cut(s)	611, 776, 853
AfaI	GTAC	3 cut(s)	51, 430, 608
AfiI	CCNNNNNNNGG	4 cut(s)	32, 365, 679, 919
AflIII	ACRYGT	1 cut(s)	838
AgsI	TTSAA	9 cut(s)	85, 103, 243, 285, 534, 554, 717, 725, 757
AjnI	CCWGG	2 cut(s)	386, 394
AloI	GAACNNNNNNTCC	2 cut(s)	27, 59
AluBI	AGCT	7 cut(s)	515, 587, 728, 887, 940, 1009, 1051
AluI	AGCT	7 cut(s)	515, 587, 728, 887, 940, 1009, 1051
Alw21I	GWGCWC	1 cut(s)	942
AlwNI	CAGNNNCTG	1 cut(s)	341
Aor13HI	TCCGGA	1 cut(s)	358
AoxI	GGCC	1 cut(s)	89
ApoI	RAATTY	3 cut(s)	58, 316, 1024
Asp700I	GAANNNNTTC	1 cut(s)	832
Asp718I	GGTACC	1 cut(s)	428
AsuHPI	GGTGA	1 cut(s)	735
BanI	GGYRCC	2 cut(s)	368, 428
BanII	GRGCYC	1 cut(s)	942
BbsI	GAAGAC	1 cut(s)	793
Bbv12I	GWGCWC	1 cut(s)	942
BccI	CCATC	4 cut(s)	434, 493, 610, 987
BciT130I	CCWGG	2 cut(s)	388, 396
BfaI	CTAG	1 cut(s)	642
BfmI	CTRYAG	1 cut(s)	651
BisI	GCNGC	1 cut(s)	231
BlsI	GCNGC	1 cut(s)	232
Bme1390I	CCNGG	2 cut(s)	388, 396
BmiI	GGNNCC	4 cut(s)	370, 430, 687, 924
BmrFI	CCNGG	2 cut(s)	388, 396
BmsI	GCATC	3 cut(s)	154, 691, 749
BpiI	GAAGAC	1 cut(s)	793
Bsa29I	ATCGAT	1 cut(s)	773
BsaJI	CCNNGG	1 cut(s)	92
BsaWI	WCCGGW	2 cut(s)	358, 612
BsaXI	ACNNNNNCTCC	4 cut(s)	27, 57, 399, 429
Bsc4I	CCNNNNNNNGG	4 cut(s)	32, 365, 679, 919
Bse118I	RCCGGY	1 cut(s)	87
Bse3DI	GCAATG	2 cut(s)	336, 1041
BseAI	TCCGGA	1 cut(s)	358
BseBI	CCWGG	2 cut(s)	388, 396
BseCI	ATCGAT	1 cut(s)	773
BseDI	CCNNGG	1 cut(s)	92
BseGI	GGATG	8 cut(s)	166, 367, 445, 621, 625, 706, 988, 998
BseLI	CCNNNNNNNGG	4 cut(s)	32, 365, 679, 919
BseMI	GCAATG	2 cut(s)	336, 1041
BseMII	CTCAG	1 cut(s)	303
BseRI	GAGGAG	1 cut(s)	695
BshFI	GGCC	1 cut(s)	91
BshNI	GGYRCC	2 cut(s)	368, 428
BshVI	ATCGAT	1 cut(s)	773
BsiHKAI	GWGCWC	1 cut(s)	942
BsiSI	CCGG	3 cut(s)	88, 359, 613
BslFI	GGGAC	1 cut(s)	363
BslI	CCNNNNNNNGG	4 cut(s)	32, 365, 679, 919
BsmFI	GGGAC	1 cut(s)	363
BsmI	GAATGC	1 cut(s)	109
BsnI	GGCC	1 cut(s)	91
Bsp1286I	GDGCHC	1 cut(s)	942
Bsp13I	TCCGGA	1 cut(s)	358
Bsp143I	GATC	2 cut(s)	770, 1019
BspACI	CCGC	2 cut(s)	26, 230
BspANI	GGCC	1 cut(s)	91
BspCNI	CTCAG	1 cut(s)	304
BspDI	ATCGAT	1 cut(s)	773
BspEI	TCCGGA	1 cut(s)	358
BspLI	GGNNCC	4 cut(s)	370, 430, 687, 924
BspQI	GCTCTTC	1 cut(s)	196
BspT107I	GGYRCC	2 cut(s)	368, 428
BsrDI	GCAATG	2 cut(s)	336, 1041
BsrFI	RCCGGY	1 cut(s)	87
BssAI	RCCGGY	1 cut(s)	87
BssECI	CCNNGG	1 cut(s)	92
BssMI	GATC	2 cut(s)	770, 1019
Bst2UI	CCWGG	2 cut(s)	388, 396
Bst4CI	ACNGT	2 cut(s)	581, 652
Bst6I	CTCTTC	1 cut(s)	196
BstC8I	GCNNGC	3 cut(s)	456, 460, 1032
BstDEI	CTNAG	2 cut(s)	312, 975
BstENI	CCTNNNNNAGG	1 cut(s)	677
BstF5I	GGATG	8 cut(s)	166, 367, 445, 621, 625, 706, 988, 998
BstKTI	GATC	2 cut(s)	773, 1022
BstMBI	GATC	2 cut(s)	770, 1019
BstMWI	GCNNNNNNNGC	1 cut(s)	212
BstNI	CCWGG	2 cut(s)	388, 396
BstNSI	RCATGY	1 cut(s)	842
BstSCI	CCNGG	2 cut(s)	386, 394
BstSFI	CTRYAG	1 cut(s)	651
BstV2I	GAAGAC	1 cut(s)	793
Bsu15I	ATCGAT	1 cut(s)	773
BsuRI	GGCC	1 cut(s)	91
BsuTUI	ATCGAT	1 cut(s)	773
BtsCI	GGATG	8 cut(s)	166, 367, 445, 621, 625, 706, 988, 998
BtsIMutI	CAGTG	1 cut(s)	812
Cac8I	GCNNGC	3 cut(s)	456, 460, 1032
CaiI	CAGNNNCTG	1 cut(s)	341
Cfr10I	RCCGGY	1 cut(s)	87
ClaI	ATCGAT	1 cut(s)	773
Csp6I	GTAC	3 cut(s)	50, 429, 607
CspCI	CAANNNNNGTGG	2 cut(s)	678, 713
CviAII	CATG	3 cut(s)	262, 290, 839
CviQI	GTAC	3 cut(s)	50, 429, 607
DdeI	CTNAG	2 cut(s)	312, 975
DpnI	GATC	2 cut(s)	772, 1021
DpnII	GATC	2 cut(s)	770, 1019
DraI	TTTAAA	1 cut(s)	732
DrdI	GACNNNNNNGTC	1 cut(s)	257
DseDI	GACNNNNNNGTC	1 cut(s)	257
Eam1104I	CTCTTC	1 cut(s)	196
EarI	CTCTTC	1 cut(s)	196
Ecl136II	GAGCTC	1 cut(s)	940
Eco24I	GRGCYC	1 cut(s)	942
Eco53kI	GAGCTC	1 cut(s)	940
Eco57I	CTGAAG	3 cut(s)	611, 776, 853
EcoICRI	GAGCTC	1 cut(s)	940
EcoNI	CCTNNNNNAGG	1 cut(s)	677
EcoRI	GAATTC	1 cut(s)	316
EcoRII	CCWGG	2 cut(s)	386, 394
EcoT38I	GRGCYC	1 cut(s)	942
FaeI	CATG	3 cut(s)	265, 293, 842
FalI	AAGNNNNNCTT	2 cut(s)	774, 806
FaqI	GGGAC	1 cut(s)	363
FatI	CATG	3 cut(s)	261, 289, 838
FauI	CCCGC	1 cut(s)	33
FauNDI	CATATG	1 cut(s)	954
Fnu4HI	GCNGC	1 cut(s)	231
FokI	GGATG	8 cut(s)	173, 374, 452, 628, 632, 713, 995, 1005
FriOI	GRGCYC	1 cut(s)	942
Fsp4HI	GCNGC	1 cut(s)	231
FspBI	CTAG	1 cut(s)	642
GluI	GCNGC	1 cut(s)	231
HaeIII	GGCC	1 cut(s)	91
HapII	CCGG	3 cut(s)	88, 359, 613
Hin1II	CATG	3 cut(s)	265, 293, 842
HindIII	AAGCTT	3 cut(s)	513, 726, 1049
HinfI	GANTC	4 cut(s)	38, 297, 399, 530
HpaII	CCGG	3 cut(s)	88, 359, 613
HphI	GGTGA	1 cut(s)	735
Hpy188I	TCNGA	3 cut(s)	407, 591, 872
Hpy188III	TCNNGA	4 cut(s)	248, 359, 642, 818
HpyAV	CCTTC	5 cut(s)	26, 730, 776, 936, 983
HpyCH4III	ACNGT	2 cut(s)	581, 652
HpyCH4V	TGCA	6 cut(s)	308, 329, 462, 470, 882, 1034
HpyF10VI	GCNNNNNNNGC	1 cut(s)	212
HpyF3I	CTNAG	2 cut(s)	312, 975
Hsp92II	CATG	3 cut(s)	265, 293, 842
Kpn2I	TCCGGA	1 cut(s)	358
KpnI	GGTACC	1 cut(s)	432
Kzo9I	GATC	2 cut(s)	770, 1019
LguI	GCTCTTC	1 cut(s)	196
LmnI	GCTCC	5 cut(s)	139, 691, 922, 937, 1014
LweI	GCATC	3 cut(s)	154, 691, 749
MaeI	CTAG	1 cut(s)	642
MaeIII	GTNAC	1 cut(s)	943
MalI	GATC	2 cut(s)	772, 1021
MboI	GATC	2 cut(s)	770, 1019
MhlI	GDGCHC	1 cut(s)	942
MluCI	AATT	9 cut(s)	58, 183, 243, 316, 660, 720, 932, 1001, 1024
MmeI	TCCRAC	2 cut(s)	117, 960
MnlI	CCTC	9 cut(s)	28, 63, 102, 151, 260, 382, 673, 676, 868
MroI	TCCGGA	1 cut(s)	358
MroXI	GAANNNNTTC	1 cut(s)	832
MseI	TTAA	3 cut(s)	111, 731, 1004
MspI	CCGG	3 cut(s)	88, 359, 613
MspR9I	CCNGG	2 cut(s)	388, 396
Mva1269I	GAATGC	1 cut(s)	109
MvaI	CCWGG	2 cut(s)	388, 396
MwoI	GCNNNNNNNGC	1 cut(s)	212
NdeI	CATATG	1 cut(s)	954
NdeII	GATC	2 cut(s)	770, 1019
NlaIII	CATG	3 cut(s)	265, 293, 842
NlaIV	GGNNCC	4 cut(s)	370, 430, 687, 924
NmeAIII	GCCGAG	1 cut(s)	73
NmuCI	GTSAC	1 cut(s)	943
NspI	RCATGY	1 cut(s)	842
PciI	ACATGT	1 cut(s)	838
PciSI	GCTCTTC	1 cut(s)	196
PctI	GAATGC	1 cut(s)	109
PdmI	GAANNNNTTC	1 cut(s)	832
PfeI	GAWTC	4 cut(s)	38, 297, 399, 530
PfoI	TCCNGGA	1 cut(s)	394
PkrI	GCNGC	1 cut(s)	232
PscI	ACATGT	1 cut(s)	838
Psp124BI	GAGCTC	1 cut(s)	942
Psp6I	CCWGG	2 cut(s)	386, 394
PspGI	CCWGG	2 cut(s)	386, 394
PspN4I	GGNNCC	4 cut(s)	370, 430, 687, 924
PstNI	CAGNNNCTG	1 cut(s)	341
RsaI	GTAC	3 cut(s)	51, 430, 608
RsaNI	GTAC	3 cut(s)	50, 429, 607
SacI	GAGCTC	1 cut(s)	942
SapI	GCTCTTC	1 cut(s)	196
SaqAI	TTAA	3 cut(s)	111, 731, 1004
SatI	GCNGC	1 cut(s)	231
Sau3AI	GATC	2 cut(s)	770, 1019
ScrFI	CCNGG	2 cut(s)	388, 396
SduI	GDGCHC	1 cut(s)	942
SfaNI	GCATC	3 cut(s)	154, 691, 749
SfcI	CTRYAG	1 cut(s)	651
Sse9I	AATT	9 cut(s)	58, 183, 243, 316, 660, 720, 932, 1001, 1024
SsiI	CCGC	2 cut(s)	26, 230
SspMI	CTAG	1 cut(s)	642
SstI	GAGCTC	1 cut(s)	942
StyD4I	CCNGG	2 cut(s)	386, 394
TaaI	ACNGT	2 cut(s)	581, 652
TaqI	TCGA	1 cut(s)	773
TasI	AATT	9 cut(s)	58, 183, 243, 316, 660, 720, 932, 1001, 1024
TauI	GCSGC	1 cut(s)	233
TfiI	GAWTC	4 cut(s)	38, 297, 399, 530
Tru1I	TTAA	3 cut(s)	111, 731, 1004
Tru9I	TTAA	3 cut(s)	111, 731, 1004
TscAI	CASTG	1 cut(s)	812
TseFI	GTSAC	1 cut(s)	943
Tsp45I	GTSAC	1 cut(s)	943
TspDTI	ATGAA	1 cut(s)	1002
TspGWI	ACGGA	2 cut(s)	290, 708
TspRI	CASTG	1 cut(s)	812
XagI	CCTNNNNNAGG	1 cut(s)	677
XapI	RAATTY	3 cut(s)	58, 316, 1024
XbaI	TCTAGA	1 cut(s)	641
XceI	RCATGY	1 cut(s)	842
XmnI	GAANNNNTTC	1 cut(s)	832
XspI	CTAG	1 cut(s)	642

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.