Belongs to the enoyl-CoA hydratase isomerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 431, 530, 626 |
| AccI | GTMKAC | 1 cut(s) | 455 |
| AccII | CGCG | 1 cut(s) | 16 |
| AciI | CCGC | 5 cut(s) | 14, 50, 378, 389, 810 |
| AclWI | GGATC | 1 cut(s) | 1305 |
| AcsI | RAATTY | 1 cut(s) | 201 |
| AcuI | CTGAAG | 3 cut(s) | 87, 1040, 1141 |
| AcyI | GRCGYC | 2 cut(s) | 531, 1028 |
| AfaI | GTAC | 1 cut(s) | 1112 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 623, 1024, 1207 |
| AgsI | TTSAA | 4 cut(s) | 412, 795, 1013, 1045 |
| AjnI | CCWGG | 2 cut(s) | 492, 652 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 254, 286 |
| AluBI | AGCT | 9 cut(s) | 292, 602, 773, 836, 866, 964, 1175, 1300, 1342 |
| AluI | AGCT | 9 cut(s) | 292, 602, 773, 836, 866, 964, 1175, 1300, 1342 |
| Alw21I | GWGCWC | 2 cut(s) | 507, 952 |
| AlwI | GGATC | 1 cut(s) | 1305 |
| Ama87I | CYCGRG | 1 cut(s) | 605 |
| AoxI | GGCC | 2 cut(s) | 248, 756 |
| ApoI | RAATTY | 1 cut(s) | 201 |
| AseI | ATTAAT | 1 cut(s) | 843 |
| AspLEI | GCGC | 2 cut(s) | 152, 533 |
| AspS9I | GGNCC | 1 cut(s) | 701 |
| AsuHPI | GGTGA | 3 cut(s) | 392, 824, 1286 |
| AsuII | TTCGAA | 1 cut(s) | 276 |
| AvaI | CYCGRG | 1 cut(s) | 605 |
| AvaII | GGWCC | 1 cut(s) | 701 |
| AxyI | CCTNAGG | 1 cut(s) | 460 |
| BaeGI | GKGCMC | 1 cut(s) | 436 |
| BanI | GGYRCC | 3 cut(s) | 431, 530, 626 |
| BbsI | GAAGAC | 1 cut(s) | 993 |
| Bbv12I | GWGCWC | 2 cut(s) | 507, 952 |
| BbvCI | CCTCAGC | 1 cut(s) | 745 |
| BccI | CCATC | 2 cut(s) | 1237, 1265 |
| BciT130I | CCWGG | 2 cut(s) | 494, 654 |
| BclI | TGATCA | 2 cut(s) | 196, 1249 |
| BfaI | CTAG | 2 cut(s) | 690, 776 |
| BfmI | CTRYAG | 1 cut(s) | 115 |
| BfoI | RGCGCY | 1 cut(s) | 534 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 755 |
| Bme1390I | CCNGG | 2 cut(s) | 494, 654 |
| Bme18I | GGWCC | 1 cut(s) | 701 |
| BmeT110I | CYCGRG | 1 cut(s) | 605 |
| BmgT120I | GGNCC | 1 cut(s) | 701 |
| BmiI | GGNNCC | 4 cut(s) | 433, 532, 628, 1212 |
| BmrFI | CCNGG | 2 cut(s) | 494, 654 |
| BmsI | GCATC | 3 cut(s) | 313, 846, 1068 |
| BoxI | GACNNNNGTC | 1 cut(s) | 15 |
| BpiI | GAAGAC | 1 cut(s) | 993 |
| BpmI | CTGGAG | 1 cut(s) | 442 |
| Bpu10I | CCTNAGC | 1 cut(s) | 745 |
| Bpu14I | TTCGAA | 1 cut(s) | 276 |
| BpuEI | CTTGAG | 1 cut(s) | 974 |
| Bsa29I | ATCGAT | 2 cut(s) | 1007, 1061 |
| BsaHI | GRCGYC | 2 cut(s) | 531, 1028 |
| BsaJI | CCNNGG | 2 cut(s) | 251, 492 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 254, 284, 415, 445 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 623, 1024, 1207 |
| Bse118I | RCCGGY | 1 cut(s) | 374 |
| Bse1I | ACTGG | 2 cut(s) | 425, 688 |
| Bse21I | CCTNAGG | 1 cut(s) | 460 |
| Bse3DI | GCAATG | 1 cut(s) | 1332 |
| BseBI | CCWGG | 2 cut(s) | 494, 654 |
| BseCI | ATCGAT | 2 cut(s) | 1007, 1061 |
| BseDI | CCNNGG | 2 cut(s) | 251, 492 |
| BseGI | GGATG | 5 cut(s) | 193, 237, 904, 1083, 1276 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 623, 1024, 1207 |
| BseMI | GCAATG | 1 cut(s) | 1332 |
| BseMII | CTCAG | 3 cut(s) | 302, 561, 759 |
| BseNI | ACTGG | 2 cut(s) | 425, 688 |
| BseRI | GAGGAG | 1 cut(s) | 1152 |
| BseSI | GKGCMC | 1 cut(s) | 436 |
| Bsh1236I | CGCG | 1 cut(s) | 16 |
| BshFI | GGCC | 2 cut(s) | 250, 758 |
| BshNI | GGYRCC | 3 cut(s) | 431, 530, 626 |
| BshVI | ATCGAT | 2 cut(s) | 1007, 1061 |
| BsiHKAI | GWGCWC | 2 cut(s) | 507, 952 |
| BsiHKCI | CYCGRG | 1 cut(s) | 605 |
| BsiSI | CCGG | 3 cut(s) | 375, 534, 593 |
| BslFI | GGGAC | 2 cut(s) | 68, 280 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 623, 1024, 1207 |
| BsmFI | GGGAC | 2 cut(s) | 68, 280 |
| BsnI | GGCC | 2 cut(s) | 250, 758 |
| BsoBI | CYCGRG | 1 cut(s) | 605 |
| Bsp119I | TTCGAA | 1 cut(s) | 276 |
| Bsp1286I | GDGCHC | 3 cut(s) | 436, 507, 952 |
| Bsp143I | GATC | 5 cut(s) | 196, 926, 1058, 1249, 1310 |
| BspACI | CCGC | 5 cut(s) | 14, 50, 378, 389, 810 |
| BspANI | GGCC | 2 cut(s) | 250, 758 |
| BspCNI | CTCAG | 3 cut(s) | 301, 562, 758 |
| BspDI | ATCGAT | 2 cut(s) | 1007, 1061 |
| BspFNI | CGCG | 1 cut(s) | 16 |
| BspLI | GGNNCC | 4 cut(s) | 433, 532, 628, 1212 |
| BspPI | GGATC | 1 cut(s) | 1305 |
| BspQI | GCTCTTC | 1 cut(s) | 355 |
| BspT104I | TTCGAA | 1 cut(s) | 276 |
| BspT107I | GGYRCC | 3 cut(s) | 431, 530, 626 |
| BsrDI | GCAATG | 1 cut(s) | 1332 |
| BsrFI | RCCGGY | 1 cut(s) | 374 |
| BsrI | ACTGG | 2 cut(s) | 425, 688 |
| BssAI | RCCGGY | 1 cut(s) | 374 |
| BssECI | CCNNGG | 2 cut(s) | 251, 492 |
| BssMI | GATC | 5 cut(s) | 196, 926, 1058, 1249, 1310 |
| BssNAI | GTATAC | 1 cut(s) | 456 |
| BssNI | GRCGYC | 2 cut(s) | 531, 1028 |
| Bst1107I | GTATAC | 1 cut(s) | 456 |
| Bst2UI | CCWGG | 2 cut(s) | 494, 654 |
| Bst4CI | ACNGT | 2 cut(s) | 551, 701 |
| Bst6I | CTCTTC | 4 cut(s) | 51, 355, 637, 1284 |
| BstACI | GRCGYC | 2 cut(s) | 531, 1028 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 371 |
| BstBI | TTCGAA | 1 cut(s) | 276 |
| BstC8I | GCNNGC | 5 cut(s) | 62, 376, 714, 886, 966 |
| BstDEI | CTNAG | 5 cut(s) | 288, 460, 570, 745, 921 |
| BstEII | GGTNACC | 1 cut(s) | 812 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1022 |
| BstF5I | GGATG | 5 cut(s) | 193, 237, 904, 1083, 1276 |
| BstFNI | CGCG | 1 cut(s) | 16 |
| BstH2I | RGCGCY | 1 cut(s) | 534 |
| BstHHI | GCGC | 2 cut(s) | 152, 533 |
| BstKTI | GATC | 5 cut(s) | 199, 929, 1061, 1252, 1313 |
| BstMBI | GATC | 5 cut(s) | 196, 926, 1058, 1249, 1310 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 289, 371, 395, 755 |
| BstNI | CCWGG | 2 cut(s) | 494, 654 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 15 |
| BstPI | GGTNACC | 1 cut(s) | 812 |
| BstSCI | CCNGG | 2 cut(s) | 492, 652 |
| BstSFI | CTRYAG | 1 cut(s) | 115 |
| BstSLI | GKGCMC | 1 cut(s) | 436 |
| BstUI | CGCG | 1 cut(s) | 16 |
| BstV2I | GAAGAC | 1 cut(s) | 993 |
| BstX2I | RGATCY | 1 cut(s) | 1310 |
| BstYI | RGATCY | 1 cut(s) | 1310 |
| BstZ17I | GTATAC | 1 cut(s) | 456 |
| Bsu15I | ATCGAT | 2 cut(s) | 1007, 1061 |
| Bsu36I | CCTNAGG | 1 cut(s) | 460 |
| BsuRI | GGCC | 2 cut(s) | 250, 758 |
| BsuTUI | ATCGAT | 2 cut(s) | 1007, 1061 |
| BtsCI | GGATG | 5 cut(s) | 193, 237, 904, 1083, 1276 |
| BtsIMutI | CAGTG | 3 cut(s) | 507, 936, 1233 |
| Cac8I | GCNNGC | 5 cut(s) | 62, 376, 714, 886, 966 |
| CfoI | GCGC | 2 cut(s) | 152, 533 |
| Cfr10I | RCCGGY | 1 cut(s) | 374 |
| Cfr13I | GGNCC | 1 cut(s) | 701 |
| ClaI | ATCGAT | 2 cut(s) | 1007, 1061 |
| CseI | GACGC | 2 cut(s) | 5, 1017 |
| Csp6I | GTAC | 1 cut(s) | 1111 |
| CviAII | CATG | 2 cut(s) | 520, 1127 |
| CviQI | GTAC | 1 cut(s) | 1111 |
| DdeI | CTNAG | 5 cut(s) | 288, 460, 570, 745, 921 |
| DinI | GGCGCC | 1 cut(s) | 532 |
| DpnI | GATC | 5 cut(s) | 198, 928, 1060, 1251, 1312 |
| DpnII | GATC | 5 cut(s) | 196, 926, 1058, 1249, 1310 |
| Eam1104I | CTCTTC | 4 cut(s) | 51, 355, 637, 1284 |
| EarI | CTCTTC | 4 cut(s) | 51, 355, 637, 1284 |
| Eco147I | AGGCCT | 1 cut(s) | 250 |
| Eco47I | GGWCC | 1 cut(s) | 701 |
| Eco57I | CTGAAG | 3 cut(s) | 87, 1040, 1141 |
| Eco81I | CCTNAGG | 1 cut(s) | 460 |
| Eco88I | CYCGRG | 1 cut(s) | 605 |
| Eco91I | GGTNACC | 1 cut(s) | 812 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1022 |
| EcoO65I | GGTNACC | 1 cut(s) | 812 |
| EcoRII | CCWGG | 2 cut(s) | 492, 652 |
| EgeI | GGCGCC | 1 cut(s) | 532 |
| EheI | GGCGCC | 1 cut(s) | 532 |
| FaeI | CATG | 2 cut(s) | 523, 1130 |
| FaqI | GGGAC | 2 cut(s) | 68, 280 |
| FatI | CATG | 2 cut(s) | 519, 1126 |
| FauI | CCCGC | 1 cut(s) | 21 |
| FbaI | TGATCA | 2 cut(s) | 196, 1249 |
| FblI | GTMKAC | 1 cut(s) | 455 |
| FokI | GGATG | 5 cut(s) | 200, 244, 911, 1090, 1283 |
| FspBI | CTAG | 2 cut(s) | 690, 776 |
| GlaI | GCGC | 2 cut(s) | 151, 532 |
| GsuI | CTGGAG | 1 cut(s) | 442 |
| HaeII | RGCGCY | 1 cut(s) | 534 |
| HaeIII | GGCC | 2 cut(s) | 250, 758 |
| HapII | CCGG | 3 cut(s) | 375, 534, 593 |
| HgaI | GACGC | 2 cut(s) | 5, 1017 |
| HhaI | GCGC | 2 cut(s) | 152, 533 |
| Hin1I | GRCGYC | 2 cut(s) | 531, 1028 |
| Hin1II | CATG | 2 cut(s) | 523, 1130 |
| Hin6I | GCGC | 2 cut(s) | 150, 531 |
| HinP1I | GCGC | 2 cut(s) | 150, 531 |
| HincII | GTYRAC | 1 cut(s) | 237 |
| HindII | GTYRAC | 1 cut(s) | 237 |
| HindIII | AAGCTT | 1 cut(s) | 1340 |
| HinfI | GANTC | 5 cut(s) | 5, 103, 657, 1013, 1063 |
| HpaII | CCGG | 3 cut(s) | 375, 534, 593 |
| HphI | GGTGA | 3 cut(s) | 392, 824, 1286 |
| Hpy166II | GTNNAC | 2 cut(s) | 237, 456 |
| Hpy188I | TCNGA | 2 cut(s) | 10, 187 |
| Hpy188III | TCNNGA | 6 cut(s) | 617, 704, 953, 1067, 1106, 1261 |
| Hpy8I | GTNNAC | 2 cut(s) | 237, 456 |
| Hpy99I | CGWCG | 1 cut(s) | 1033 |
| HpyAV | CCTTC | 5 cut(s) | 283, 952, 1018, 1064, 1068 |
| HpyCH4III | ACNGT | 2 cut(s) | 551, 701 |
| HpyCH4V | TGCA | 2 cut(s) | 859, 1081 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 289, 371, 395, 755 |
| HpyF3I | CTNAG | 5 cut(s) | 288, 460, 570, 745, 921 |
| Hsp92I | GRCGYC | 2 cut(s) | 531, 1028 |
| Hsp92II | CATG | 2 cut(s) | 523, 1130 |
| HspAI | GCGC | 2 cut(s) | 150, 531 |
| KasI | GGCGCC | 1 cut(s) | 530 |
| KroI | GCCGGC | 1 cut(s) | 374 |
| KroNI | GCCGGC | 1 cut(s) | 376 |
| Ksp22I | TGATCA | 2 cut(s) | 196, 1249 |
| Kzo9I | GATC | 5 cut(s) | 196, 926, 1058, 1249, 1310 |
| LguI | GCTCTTC | 1 cut(s) | 355 |
| LmnI | GCTCC | 3 cut(s) | 961, 1210, 1305 |
| LweI | GCATC | 3 cut(s) | 313, 846, 1068 |
| MaeI | CTAG | 2 cut(s) | 690, 776 |
| MaeIII | GTNAC | 1 cut(s) | 812 |
| MalI | GATC | 5 cut(s) | 198, 928, 1060, 1251, 1312 |
| MboI | GATC | 5 cut(s) | 196, 926, 1058, 1249, 1310 |
| MflI | RGATCY | 1 cut(s) | 1310 |
| MhlI | GDGCHC | 3 cut(s) | 436, 507, 952 |
| MluCI | AATT | 6 cut(s) | 201, 513, 752, 786, 1283, 1315 |
| Mly113I | GGCGCC | 1 cut(s) | 531 |
| MmeI | TCCRAC | 3 cut(s) | 1164, 1203, 1248 |
| MroNI | GCCGGC | 1 cut(s) | 374 |
| MseI | TTAA | 4 cut(s) | 843, 944, 1295, 1345 |
| MspA1I | CMGCKG | 1 cut(s) | 292 |
| MspI | CCGG | 3 cut(s) | 375, 534, 593 |
| MspR9I | CCNGG | 2 cut(s) | 494, 654 |
| MvaI | CCWGG | 2 cut(s) | 494, 654 |
| MvnI | CGCG | 1 cut(s) | 16 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 289, 371, 395, 755 |
| NaeI | GCCGGC | 1 cut(s) | 376 |
| NarI | GGCGCC | 1 cut(s) | 531 |
| NdeII | GATC | 5 cut(s) | 196, 926, 1058, 1249, 1310 |
| NgoMIV | GCCGGC | 1 cut(s) | 374 |
| NlaIII | CATG | 2 cut(s) | 523, 1130 |
| NlaIV | GGNNCC | 4 cut(s) | 433, 532, 628, 1212 |
| NmeAIII | GCCGAG | 1 cut(s) | 232 |
| NmuCI | GTSAC | 1 cut(s) | 812 |
| NspV | TTCGAA | 1 cut(s) | 276 |
| PceI | AGGCCT | 1 cut(s) | 250 |
| PciSI | GCTCTTC | 1 cut(s) | 355 |
| PdiI | GCCGGC | 1 cut(s) | 376 |
| PfeI | GAWTC | 5 cut(s) | 5, 103, 657, 1013, 1063 |
| PfoI | TCCNGGA | 1 cut(s) | 652 |
| PluTI | GGCGCC | 1 cut(s) | 534 |
| PshAI | GACNNNNGTC | 1 cut(s) | 15 |
| PshBI | ATTAAT | 1 cut(s) | 843 |
| Psp6I | CCWGG | 2 cut(s) | 492, 652 |
| PspEI | GGTNACC | 1 cut(s) | 812 |
| PspGI | CCWGG | 2 cut(s) | 492, 652 |
| PspN4I | GGNNCC | 4 cut(s) | 433, 532, 628, 1212 |
| PspPI | GGNCC | 1 cut(s) | 701 |
| PsuI | RGATCY | 1 cut(s) | 1310 |
| PvuII | CAGCTG | 1 cut(s) | 292 |
| RsaI | GTAC | 1 cut(s) | 1112 |
| RsaNI | GTAC | 1 cut(s) | 1111 |
| SapI | GCTCTTC | 1 cut(s) | 355 |
| SaqAI | TTAA | 4 cut(s) | 843, 944, 1295, 1345 |
| Sau3AI | GATC | 5 cut(s) | 196, 926, 1058, 1249, 1310 |
| Sau96I | GGNCC | 1 cut(s) | 701 |
| ScrFI | CCNGG | 2 cut(s) | 494, 654 |
| SduI | GDGCHC | 3 cut(s) | 436, 507, 952 |
| SfaNI | GCATC | 3 cut(s) | 313, 846, 1068 |
| SfcI | CTRYAG | 1 cut(s) | 115 |
| SfoI | GGCGCC | 1 cut(s) | 532 |
| SfuI | TTCGAA | 1 cut(s) | 276 |
| SinI | GGWCC | 1 cut(s) | 701 |
| SmlI | CTYRAG | 1 cut(s) | 953 |
| SmoI | CTYRAG | 1 cut(s) | 953 |
| Sse9I | AATT | 6 cut(s) | 201, 513, 752, 786, 1283, 1315 |
| SseBI | AGGCCT | 1 cut(s) | 250 |
| SsiI | CCGC | 5 cut(s) | 14, 50, 378, 389, 810 |
| SspDI | GGCGCC | 1 cut(s) | 530 |
| SspI | AATATT | 2 cut(s) | 829, 942 |
| SspMI | CTAG | 2 cut(s) | 690, 776 |
| StuI | AGGCCT | 1 cut(s) | 250 |
| StyD4I | CCNGG | 2 cut(s) | 492, 652 |
| TaaI | ACNGT | 2 cut(s) | 551, 701 |
| TaqI | TCGA | 6 cut(s) | 276, 854, 1007, 1061, 1066, 1262 |
| TasI | AATT | 6 cut(s) | 201, 513, 752, 786, 1283, 1315 |
| TatI | WGTACW | 1 cut(s) | 1110 |
| TfiI | GAWTC | 5 cut(s) | 5, 103, 657, 1013, 1063 |
| Tru1I | TTAA | 4 cut(s) | 843, 944, 1295, 1345 |
| Tru9I | TTAA | 4 cut(s) | 843, 944, 1295, 1345 |
| TscAI | CASTG | 3 cut(s) | 507, 936, 1240 |
| TseFI | GTSAC | 1 cut(s) | 812 |
| Tsp45I | GTSAC | 1 cut(s) | 812 |
| TspDTI | ATGAA | 4 cut(s) | 166, 204, 993, 1293 |
| TspRI | CASTG | 3 cut(s) | 507, 936, 1240 |
| VpaK11BI | GGWCC | 1 cut(s) | 701 |
| VspI | ATTAAT | 1 cut(s) | 843 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1022 |
| XapI | RAATTY | 1 cut(s) | 201 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 334 |
| XmiI | GTMKAC | 1 cut(s) | 455 |
| XspI | CTAG | 2 cut(s) | 690, 776 |