Belongs to the enoyl-CoA hydratase isomerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 107 |
| AcsI | RAATTY | 2 cut(s) | 18, 307 |
| AcuI | CTGAAG | 1 cut(s) | 407 |
| AfaI | GTAC | 2 cut(s) | 479, 694 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 391, 574 |
| AgsI | TTSAA | 2 cut(s) | 276, 412 |
| AjnI | CCWGG | 1 cut(s) | 133 |
| AluBI | AGCT | 7 cut(s) | 14, 254, 331, 542, 595, 667, 709 |
| AluI | AGCT | 7 cut(s) | 14, 254, 331, 542, 595, 667, 709 |
| Alw21I | GWGCWC | 1 cut(s) | 319 |
| AoxI | GGCC | 1 cut(s) | 237 |
| ApeKI | GCWGC | 1 cut(s) | 667 |
| ApoI | RAATTY | 2 cut(s) | 18, 307 |
| BanI | GGYRCC | 1 cut(s) | 107 |
| BbsI | GAAGAC | 1 cut(s) | 360 |
| Bbv12I | GWGCWC | 1 cut(s) | 319 |
| BbvI | GCAGC | 1 cut(s) | 654 |
| BccI | CCATC | 1 cut(s) | 604 |
| BciT130I | CCWGG | 1 cut(s) | 135 |
| BclI | TGATCA | 1 cut(s) | 616 |
| BfaI | CTAG | 3 cut(s) | 98, 171, 257 |
| BglII | AGATCT | 1 cut(s) | 677 |
| BisI | GCNGC | 1 cut(s) | 668 |
| BlpI | GCTNAGC | 1 cut(s) | 705 |
| BlsI | GCNGC | 1 cut(s) | 669 |
| Bme1390I | CCNGG | 1 cut(s) | 135 |
| BmiI | GGNNCC | 2 cut(s) | 109, 579 |
| BmrFI | CCNGG | 1 cut(s) | 135 |
| BmsI | GCATC | 2 cut(s) | 404, 435 |
| BpiI | GAAGAC | 1 cut(s) | 360 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 705 |
| BpuEI | CTTGAG | 1 cut(s) | 341 |
| Bsa29I | ATCGAT | 2 cut(s) | 374, 428 |
| BsaJI | CCNNGG | 1 cut(s) | 298 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 391, 574 |
| Bse1I | ACTGG | 1 cut(s) | 169 |
| Bse3DI | GCAATG | 1 cut(s) | 699 |
| BseBI | CCWGG | 1 cut(s) | 135 |
| BseCI | ATCGAT | 2 cut(s) | 374, 428 |
| BseDI | CCNNGG | 1 cut(s) | 298 |
| BseGI | GGATG | 1 cut(s) | 450 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 391, 574 |
| BseMI | GCAATG | 1 cut(s) | 699 |
| BseMII | CTCAG | 1 cut(s) | 42 |
| BseNI | ACTGG | 1 cut(s) | 169 |
| BseXI | GCAGC | 1 cut(s) | 654 |
| BshFI | GGCC | 1 cut(s) | 239 |
| BshNI | GGYRCC | 1 cut(s) | 107 |
| BshVI | ATCGAT | 2 cut(s) | 374, 428 |
| BsiHKAI | GWGCWC | 1 cut(s) | 319 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 391, 574 |
| BsnI | GGCC | 1 cut(s) | 239 |
| Bsp1286I | GDGCHC | 1 cut(s) | 319 |
| Bsp143I | GATC | 3 cut(s) | 425, 616, 677 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 705 |
| BspANI | GGCC | 1 cut(s) | 239 |
| BspCNI | CTCAG | 1 cut(s) | 43 |
| BspDI | ATCGAT | 2 cut(s) | 374, 428 |
| BspHI | TCATGA | 1 cut(s) | 472 |
| BspLI | GGNNCC | 2 cut(s) | 109, 579 |
| BspT107I | GGYRCC | 1 cut(s) | 107 |
| BsrDI | GCAATG | 1 cut(s) | 699 |
| BsrI | ACTGG | 1 cut(s) | 169 |
| BssECI | CCNNGG | 1 cut(s) | 298 |
| BssMI | GATC | 3 cut(s) | 425, 616, 677 |
| BssT1I | CCWWGG | 1 cut(s) | 298 |
| Bst2UI | CCWGG | 1 cut(s) | 135 |
| Bst4CI | ACNGT | 2 cut(s) | 32, 182 |
| Bst6I | CTCTTC | 1 cut(s) | 651 |
| BstC8I | GCNNGC | 2 cut(s) | 195, 333 |
| BstDEI | CTNAG | 3 cut(s) | 51, 585, 705 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 389 |
| BstF5I | GGATG | 1 cut(s) | 450 |
| BstKTI | GATC | 3 cut(s) | 428, 619, 680 |
| BstMBI | GATC | 3 cut(s) | 425, 616, 677 |
| BstNI | CCWGG | 1 cut(s) | 135 |
| BstSCI | CCNGG | 1 cut(s) | 133 |
| BstV1I | GCAGC | 1 cut(s) | 654 |
| BstV2I | GAAGAC | 1 cut(s) | 360 |
| BstX2I | RGATCY | 1 cut(s) | 677 |
| BstYI | RGATCY | 1 cut(s) | 677 |
| Bsu15I | ATCGAT | 2 cut(s) | 374, 428 |
| BsuRI | GGCC | 1 cut(s) | 239 |
| BsuTUI | ATCGAT | 2 cut(s) | 374, 428 |
| BtgZI | GCGATG | 1 cut(s) | 381 |
| BtsCI | GGATG | 1 cut(s) | 450 |
| BtsI | GCAGTG | 1 cut(s) | 298 |
| BtsIMutI | CAGTG | 2 cut(s) | 298, 600 |
| Cac8I | GCNNGC | 2 cut(s) | 195, 333 |
| CciI | TCATGA | 1 cut(s) | 472 |
| ClaI | ATCGAT | 2 cut(s) | 374, 428 |
| Csp6I | GTAC | 2 cut(s) | 478, 693 |
| CviAII | CATG | 2 cut(s) | 473, 494 |
| CviQI | GTAC | 2 cut(s) | 478, 693 |
| DdeI | CTNAG | 3 cut(s) | 51, 585, 705 |
| DpnI | GATC | 3 cut(s) | 427, 618, 679 |
| DpnII | GATC | 3 cut(s) | 425, 616, 677 |
| DraI | TTTAAA | 1 cut(s) | 348 |
| Eam1104I | CTCTTC | 1 cut(s) | 651 |
| EarI | CTCTTC | 1 cut(s) | 651 |
| Eco130I | CCWWGG | 1 cut(s) | 298 |
| Eco57I | CTGAAG | 1 cut(s) | 407 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 389 |
| EcoRII | CCWGG | 1 cut(s) | 133 |
| EcoT14I | CCWWGG | 1 cut(s) | 298 |
| ErhI | CCWWGG | 1 cut(s) | 298 |
| FaeI | CATG | 2 cut(s) | 476, 497 |
| FaiI | YATR | 8 cut(s) | 26, 71, 284, 474, 495, 500, 545, 690 |
| FatI | CATG | 2 cut(s) | 472, 493 |
| FbaI | TGATCA | 1 cut(s) | 616 |
| Fnu4HI | GCNGC | 1 cut(s) | 668 |
| FokI | GGATG | 1 cut(s) | 457 |
| Fsp4HI | GCNGC | 1 cut(s) | 668 |
| FspBI | CTAG | 3 cut(s) | 98, 171, 257 |
| GluI | GCNGC | 1 cut(s) | 668 |
| HaeIII | GGCC | 1 cut(s) | 239 |
| Hin1II | CATG | 2 cut(s) | 476, 497 |
| HindIII | AAGCTT | 1 cut(s) | 707 |
| HinfI | GANTC | 2 cut(s) | 138, 430 |
| Hpy188I | TCNGA | 1 cut(s) | 187 |
| Hpy188III | TCNNGA | 6 cut(s) | 98, 320, 356, 434, 473, 628 |
| HpyAV | CCTTC | 5 cut(s) | 319, 385, 431, 435, 591 |
| HpyCH4III | ACNGT | 2 cut(s) | 32, 182 |
| HpyCH4V | TGCA | 1 cut(s) | 448 |
| HpyF3I | CTNAG | 3 cut(s) | 51, 585, 705 |
| Hsp92II | CATG | 2 cut(s) | 476, 497 |
| Ksp22I | TGATCA | 1 cut(s) | 616 |
| Kzo9I | GATC | 3 cut(s) | 425, 616, 677 |
| LmnI | GCTCC | 4 cut(s) | 11, 328, 577, 592 |
| LpnPI | CCDG | 5 cut(s) | 120, 147, 150, 398, 684 |
| Lsp1109I | GCAGC | 1 cut(s) | 654 |
| LweI | GCATC | 2 cut(s) | 404, 435 |
| MaeI | CTAG | 3 cut(s) | 98, 171, 257 |
| MaeIII | GTNAC | 1 cut(s) | 293 |
| MalI | GATC | 3 cut(s) | 427, 618, 679 |
| MboI | GATC | 3 cut(s) | 425, 616, 677 |
| MboII | GAAGA | 6 cut(s) | 260, 360, 400, 424, 659, 668 |
| MflI | RGATCY | 1 cut(s) | 677 |
| MhlI | GDGCHC | 1 cut(s) | 319 |
| MluCI | AATT | 5 cut(s) | 18, 233, 267, 307, 682 |
| MmeI | TCCRAC | 2 cut(s) | 531, 570 |
| MnlI | CCTC | 6 cut(s) | 121, 235, 250, 296, 497, 523 |
| MseI | TTAA | 5 cut(s) | 311, 347, 378, 662, 712 |
| MspR9I | CCNGG | 1 cut(s) | 135 |
| MvaI | CCWGG | 1 cut(s) | 135 |
| NdeII | GATC | 3 cut(s) | 425, 616, 677 |
| NlaIII | CATG | 2 cut(s) | 476, 497 |
| NlaIV | GGNNCC | 2 cut(s) | 109, 579 |
| NmuCI | GTSAC | 1 cut(s) | 293 |
| PagI | TCATGA | 1 cut(s) | 472 |
| PfeI | GAWTC | 2 cut(s) | 138, 430 |
| PfoI | TCCNGGA | 1 cut(s) | 133 |
| PkrI | GCNGC | 1 cut(s) | 669 |
| Psp6I | CCWGG | 1 cut(s) | 133 |
| PspGI | CCWGG | 1 cut(s) | 133 |
| PspN4I | GGNNCC | 2 cut(s) | 109, 579 |
| PsuI | RGATCY | 1 cut(s) | 677 |
| RsaI | GTAC | 2 cut(s) | 479, 694 |
| RsaNI | GTAC | 2 cut(s) | 478, 693 |
| SaqAI | TTAA | 5 cut(s) | 311, 347, 378, 662, 712 |
| SatI | GCNGC | 1 cut(s) | 668 |
| Sau3AI | GATC | 3 cut(s) | 425, 616, 677 |
| ScrFI | CCNGG | 1 cut(s) | 135 |
| SduI | GDGCHC | 1 cut(s) | 319 |
| SfaNI | GCATC | 2 cut(s) | 404, 435 |
| SmlI | CTYRAG | 1 cut(s) | 320 |
| SmoI | CTYRAG | 1 cut(s) | 320 |
| Sse9I | AATT | 5 cut(s) | 18, 233, 267, 307, 682 |
| SspMI | CTAG | 3 cut(s) | 98, 171, 257 |
| StyD4I | CCNGG | 1 cut(s) | 133 |
| StyI | CCWWGG | 1 cut(s) | 298 |
| TaaI | ACNGT | 2 cut(s) | 32, 182 |
| TaqI | TCGA | 4 cut(s) | 374, 428, 433, 629 |
| TasI | AATT | 5 cut(s) | 18, 233, 267, 307, 682 |
| TatI | WGTACW | 1 cut(s) | 477 |
| TfiI | GAWTC | 2 cut(s) | 138, 430 |
| Tru1I | TTAA | 5 cut(s) | 311, 347, 378, 662, 712 |
| Tru9I | TTAA | 5 cut(s) | 311, 347, 378, 662, 712 |
| TscAI | CASTG | 2 cut(s) | 298, 607 |
| TseFI | GTSAC | 1 cut(s) | 293 |
| TseI | GCWGC | 1 cut(s) | 667 |
| Tsp45I | GTSAC | 1 cut(s) | 293 |
| TspDTI | ATGAA | 3 cut(s) | 360, 461, 660 |
| TspRI | CASTG | 2 cut(s) | 298, 607 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 389 |
| XapI | RAATTY | 2 cut(s) | 18, 307 |
| XbaI | TCTAGA | 1 cut(s) | 97 |
| XspI | CTAG | 3 cut(s) | 98, 171, 257 |