Belongs to the enoyl-CoA hydratase isomerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 62 |
| Acc36I | ACCTGC | 1 cut(s) | 62 |
| AccB1I | GGYRCC | 1 cut(s) | 224 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 436 |
| AccI | GTMKAC | 1 cut(s) | 860 |
| AccII | CGCG | 1 cut(s) | 654 |
| AciI | CCGC | 1 cut(s) | 622 |
| AclWI | GGATC | 1 cut(s) | 843 |
| AcoI | YGGCCR | 1 cut(s) | 796 |
| AfaI | GTAC | 1 cut(s) | 5 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 161, 436, 496 |
| AgsI | TTSAA | 3 cut(s) | 81, 494, 589 |
| AjnI | CCWGG | 2 cut(s) | 30, 190 |
| AluBI | AGCT | 6 cut(s) | 140, 348, 407, 722, 775, 838 |
| AluI | AGCT | 6 cut(s) | 140, 348, 407, 722, 775, 838 |
| AlwI | GGATC | 1 cut(s) | 843 |
| AoxI | GGCC | 1 cut(s) | 796 |
| ApeKI | GCWGC | 2 cut(s) | 137, 625 |
| AspS9I | GGNCC | 1 cut(s) | 239 |
| AsuC2I | CCSGG | 1 cut(s) | 67 |
| AsuHPI | GGTGA | 1 cut(s) | 650 |
| AvaII | GGWCC | 1 cut(s) | 239 |
| BalI | TGGCCA | 1 cut(s) | 798 |
| BanI | GGYRCC | 1 cut(s) | 224 |
| BbvI | GCAGC | 2 cut(s) | 149, 612 |
| BccI | CCATC | 3 cut(s) | 109, 586, 784 |
| BciT130I | CCWGG | 2 cut(s) | 32, 192 |
| BclI | TGATCA | 1 cut(s) | 526 |
| BcnI | CCSGG | 1 cut(s) | 67 |
| BfaI | CTAG | 5 cut(s) | 263, 314, 383, 765, 810 |
| BfuAI | ACCTGC | 1 cut(s) | 62 |
| BisI | GCNGC | 3 cut(s) | 138, 623, 626 |
| BlsI | GCNGC | 3 cut(s) | 139, 624, 627 |
| Bme1390I | CCNGG | 3 cut(s) | 32, 67, 192 |
| Bme18I | GGWCC | 1 cut(s) | 239 |
| BmgT120I | GGNCC | 1 cut(s) | 239 |
| BmiI | GGNNCC | 3 cut(s) | 64, 166, 226 |
| BmrFI | CCNGG | 3 cut(s) | 32, 67, 192 |
| BmsI | GCATC | 2 cut(s) | 376, 581 |
| BpuEI | CTTGAG | 1 cut(s) | 334 |
| BpuMI | CCSGG | 1 cut(s) | 67 |
| Bsa29I | ATCGAT | 1 cut(s) | 605 |
| BsaJI | CCNNGG | 3 cut(s) | 30, 57, 66 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 161, 436, 496 |
| BseBI | CCWGG | 2 cut(s) | 32, 192 |
| BseCI | ATCGAT | 1 cut(s) | 605 |
| BseDI | CCNNGG | 3 cut(s) | 30, 57, 66 |
| BseGI | GGATG | 1 cut(s) | 445 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 161, 436, 496 |
| BseMII | CTCAG | 1 cut(s) | 387 |
| BseXI | GCAGC | 2 cut(s) | 149, 612 |
| Bsh1236I | CGCG | 1 cut(s) | 654 |
| BshFI | GGCC | 1 cut(s) | 798 |
| BshNI | GGYRCC | 1 cut(s) | 224 |
| BshVI | ATCGAT | 1 cut(s) | 605 |
| BsiSI | CCGG | 2 cut(s) | 66, 131 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 161, 436, 496 |
| BsnI | GGCC | 1 cut(s) | 798 |
| Bsp143I | GATC | 3 cut(s) | 526, 602, 848 |
| Bsp19I | CCATGG | 1 cut(s) | 57 |
| Bsp68I | TCGCGA | 1 cut(s) | 654 |
| BspACI | CCGC | 1 cut(s) | 622 |
| BspANI | GGCC | 1 cut(s) | 798 |
| BspCNI | CTCAG | 1 cut(s) | 388 |
| BspDI | ATCGAT | 1 cut(s) | 605 |
| BspFNI | CGCG | 1 cut(s) | 654 |
| BspHI | TCATGA | 1 cut(s) | 529 |
| BspLI | GGNNCC | 3 cut(s) | 64, 166, 226 |
| BspMI | ACCTGC | 1 cut(s) | 62 |
| BspPI | GGATC | 1 cut(s) | 843 |
| BspT107I | GGYRCC | 1 cut(s) | 224 |
| BssECI | CCNNGG | 3 cut(s) | 30, 57, 66 |
| BssMI | GATC | 3 cut(s) | 526, 602, 848 |
| BssT1I | CCWWGG | 1 cut(s) | 57 |
| Bst2UI | CCWGG | 2 cut(s) | 32, 192 |
| Bst4CI | ACNGT | 6 cut(s) | 89, 239, 422, 434, 472, 729 |
| Bst6I | CTCTTC | 1 cut(s) | 471 |
| BstC8I | GCNNGC | 1 cut(s) | 252 |
| BstDEI | CTNAG | 1 cut(s) | 396 |
| BstDSI | CCRYGG | 1 cut(s) | 57 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 494 |
| BstF5I | GGATG | 1 cut(s) | 445 |
| BstFNI | CGCG | 1 cut(s) | 654 |
| BstKTI | GATC | 3 cut(s) | 529, 605, 851 |
| BstMBI | GATC | 3 cut(s) | 526, 602, 848 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 651 |
| BstNI | CCWGG | 2 cut(s) | 32, 192 |
| BstSCI | CCNGG | 3 cut(s) | 30, 65, 190 |
| BstUI | CGCG | 1 cut(s) | 654 |
| BstV1I | GCAGC | 2 cut(s) | 149, 612 |
| BstX2I | RGATCY | 1 cut(s) | 848 |
| BstYI | RGATCY | 1 cut(s) | 848 |
| Bsu15I | ATCGAT | 1 cut(s) | 605 |
| BsuRI | GGCC | 1 cut(s) | 798 |
| BsuTUI | ATCGAT | 1 cut(s) | 605 |
| BtgI | CCRYGG | 1 cut(s) | 57 |
| BtgZI | GCGATG | 1 cut(s) | 558 |
| BtsCI | GGATG | 1 cut(s) | 445 |
| BtsIMutI | CAGTG | 3 cut(s) | 427, 477, 780 |
| BtuMI | TCGCGA | 1 cut(s) | 654 |
| BveI | ACCTGC | 1 cut(s) | 62 |
| Cac8I | GCNNGC | 1 cut(s) | 252 |
| CciI | TCATGA | 1 cut(s) | 529 |
| Cfr13I | GGNCC | 1 cut(s) | 239 |
| ClaI | ATCGAT | 1 cut(s) | 605 |
| Csp6I | GTAC | 1 cut(s) | 4 |
| CviAII | CATG | 8 cut(s) | 58, 98, 127, 362, 530, 674, 706, 818 |
| CviQI | GTAC | 1 cut(s) | 4 |
| DdeI | CTNAG | 1 cut(s) | 396 |
| DpnI | GATC | 3 cut(s) | 528, 604, 850 |
| DpnII | GATC | 3 cut(s) | 526, 602, 848 |
| EaeI | YGGCCR | 1 cut(s) | 796 |
| Eam1104I | CTCTTC | 1 cut(s) | 471 |
| EarI | CTCTTC | 1 cut(s) | 471 |
| Eco130I | CCWWGG | 1 cut(s) | 57 |
| Eco47I | GGWCC | 1 cut(s) | 239 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 494 |
| EcoRII | CCWGG | 2 cut(s) | 30, 190 |
| EcoT14I | CCWWGG | 1 cut(s) | 57 |
| EcoT22I | ATGCAT | 2 cut(s) | 391, 574 |
| ErhI | CCWWGG | 1 cut(s) | 57 |
| FaeI | CATG | 8 cut(s) | 61, 101, 130, 365, 533, 677, 709, 821 |
| FatI | CATG | 8 cut(s) | 57, 97, 126, 361, 529, 673, 705, 817 |
| FbaI | TGATCA | 1 cut(s) | 526 |
| FblI | GTMKAC | 1 cut(s) | 860 |
| Fnu4HI | GCNGC | 3 cut(s) | 138, 623, 626 |
| FokI | GGATG | 1 cut(s) | 452 |
| Fsp4HI | GCNGC | 3 cut(s) | 138, 623, 626 |
| FspBI | CTAG | 5 cut(s) | 263, 314, 383, 765, 810 |
| GluI | GCNGC | 3 cut(s) | 138, 623, 626 |
| HaeIII | GGCC | 1 cut(s) | 798 |
| HapII | CCGG | 2 cut(s) | 66, 131 |
| Hin1II | CATG | 8 cut(s) | 61, 101, 130, 365, 533, 677, 709, 821 |
| HinfI | GANTC | 2 cut(s) | 195, 607 |
| HpaII | CCGG | 2 cut(s) | 66, 131 |
| HphI | GGTGA | 1 cut(s) | 650 |
| Hpy166II | GTNNAC | 2 cut(s) | 418, 861 |
| Hpy188I | TCNGA | 4 cut(s) | 146, 376, 397, 411 |
| Hpy188III | TCNNGA | 8 cut(s) | 155, 183, 242, 383, 530, 611, 653, 765 |
| Hpy8I | GTNNAC | 2 cut(s) | 418, 861 |
| HpyAV | CCTTC | 3 cut(s) | 608, 612, 771 |
| HpyCH4III | ACNGT | 6 cut(s) | 89, 239, 422, 434, 472, 729 |
| HpyCH4V | TGCA | 5 cut(s) | 389, 572, 628, 645, 817 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 651 |
| HpyF3I | CTNAG | 1 cut(s) | 396 |
| Hsp92II | CATG | 8 cut(s) | 61, 101, 130, 365, 533, 677, 709, 821 |
| Ksp22I | TGATCA | 1 cut(s) | 526 |
| Kzo9I | GATC | 3 cut(s) | 526, 602, 848 |
| LmnI | GCTCC | 1 cut(s) | 843 |
| Lsp1109I | GCAGC | 2 cut(s) | 149, 612 |
| LweI | GCATC | 2 cut(s) | 376, 581 |
| MaeI | CTAG | 5 cut(s) | 263, 314, 383, 765, 810 |
| MaeIII | GTNAC | 1 cut(s) | 778 |
| MalI | GATC | 3 cut(s) | 528, 604, 850 |
| MboI | GATC | 3 cut(s) | 526, 602, 848 |
| MboII | GAAGA | 6 cut(s) | 124, 317, 488, 537, 601, 839 |
| MflI | RGATCY | 1 cut(s) | 848 |
| MlsI | TGGCCA | 1 cut(s) | 798 |
| MluCI | AATT | 4 cut(s) | 290, 552, 830, 854 |
| MluNI | TGGCCA | 1 cut(s) | 798 |
| MmeI | TCCRAC | 1 cut(s) | 711 |
| MnlI | CCTC | 5 cut(s) | 140, 178, 292, 703, 852 |
| Mox20I | TGGCCA | 1 cut(s) | 798 |
| Mph1103I | ATGCAT | 2 cut(s) | 391, 574 |
| MscI | TGGCCA | 1 cut(s) | 798 |
| MseI | TTAA | 3 cut(s) | 485, 512, 833 |
| Msp20I | TGGCCA | 1 cut(s) | 798 |
| MspI | CCGG | 2 cut(s) | 66, 131 |
| MspR9I | CCNGG | 3 cut(s) | 32, 67, 192 |
| MvaI | CCWGG | 2 cut(s) | 32, 192 |
| MvnI | CGCG | 1 cut(s) | 654 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 651 |
| NciI | CCSGG | 1 cut(s) | 67 |
| NcoI | CCATGG | 1 cut(s) | 57 |
| NdeII | GATC | 3 cut(s) | 526, 602, 848 |
| NlaIII | CATG | 8 cut(s) | 61, 101, 130, 365, 533, 677, 709, 821 |
| NlaIV | GGNNCC | 3 cut(s) | 64, 166, 226 |
| NmuCI | GTSAC | 1 cut(s) | 778 |
| NruI | TCGCGA | 1 cut(s) | 654 |
| NsiI | ATGCAT | 2 cut(s) | 391, 574 |
| PagI | TCATGA | 1 cut(s) | 529 |
| PaqCI | CACCTGC | 1 cut(s) | 62 |
| PfeI | GAWTC | 2 cut(s) | 195, 607 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 436 |
| PfoI | TCCNGGA | 1 cut(s) | 190 |
| PkrI | GCNGC | 3 cut(s) | 139, 624, 627 |
| Psp6I | CCWGG | 2 cut(s) | 30, 190 |
| PspGI | CCWGG | 2 cut(s) | 30, 190 |
| PspN4I | GGNNCC | 3 cut(s) | 64, 166, 226 |
| PspPI | GGNCC | 1 cut(s) | 239 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 410, 442 |
| PsuI | RGATCY | 1 cut(s) | 848 |
| RruI | TCGCGA | 1 cut(s) | 654 |
| RsaI | GTAC | 1 cut(s) | 5 |
| RsaNI | GTAC | 1 cut(s) | 4 |
| SaqAI | TTAA | 3 cut(s) | 485, 512, 833 |
| SatI | GCNGC | 3 cut(s) | 138, 623, 626 |
| Sau3AI | GATC | 3 cut(s) | 526, 602, 848 |
| Sau96I | GGNCC | 1 cut(s) | 239 |
| ScrFI | CCNGG | 3 cut(s) | 32, 67, 192 |
| SfaNI | GCATC | 2 cut(s) | 376, 581 |
| SinI | GGWCC | 1 cut(s) | 239 |
| SmlI | CTYRAG | 1 cut(s) | 349 |
| SmoI | CTYRAG | 1 cut(s) | 349 |
| Sse9I | AATT | 4 cut(s) | 290, 552, 830, 854 |
| SsiI | CCGC | 1 cut(s) | 622 |
| SspMI | CTAG | 5 cut(s) | 263, 314, 383, 765, 810 |
| StyD4I | CCNGG | 3 cut(s) | 30, 65, 190 |
| StyI | CCWWGG | 1 cut(s) | 57 |
| TaaI | ACNGT | 6 cut(s) | 89, 239, 422, 434, 472, 729 |
| TaqI | TCGA | 2 cut(s) | 605, 610 |
| TasI | AATT | 4 cut(s) | 290, 552, 830, 854 |
| TatI | WGTACW | 1 cut(s) | 3 |
| TauI | GCSGC | 1 cut(s) | 625 |
| TfiI | GAWTC | 2 cut(s) | 195, 607 |
| Tru1I | TTAA | 3 cut(s) | 485, 512, 833 |
| Tru9I | TTAA | 3 cut(s) | 485, 512, 833 |
| TscAI | CASTG | 3 cut(s) | 427, 477, 787 |
| TseFI | GTSAC | 1 cut(s) | 778 |
| TseI | GCWGC | 2 cut(s) | 137, 625 |
| Tsp45I | GTSAC | 1 cut(s) | 778 |
| TspDTI | ATGAA | 6 cut(s) | 27, 86, 537, 549, 578, 694 |
| TspGWI | ACGGA | 1 cut(s) | 122 |
| TspRI | CASTG | 3 cut(s) | 427, 477, 787 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 436 |
| VpaK11BI | GGWCC | 1 cut(s) | 239 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 494 |
| XbaI | TCTAGA | 2 cut(s) | 382, 764 |
| XmiI | GTMKAC | 1 cut(s) | 860 |
| XspI | CTAG | 5 cut(s) | 263, 314, 383, 765, 810 |
| Zsp2I | ATGCAT | 2 cut(s) | 391, 574 |